Search Results (24)

Influenza A virus (IAV) poses a significant threat to animal and public health due to its wide host range and potential for interspecies transmission. This study aimed to conduct a comprehensive survey of IAV in a wide range of wildlife in the Orinoco flooded savannas of Colombia, a region of high biodiversity and a strategic location for monitoring viral transmission. Sampling was conducted during both dry and rainy seasons in two localities. ELISA and qPCR targeted 2028 individuals from 173 bird, mammal, and reptile species, 124 of them without previously published reports. There were positive results for 54 species, with 34 representing first-time world reports. Of the qPCR, 13.4% were positive from birds, and 2.9% were from mammals. Seropositivity was identified in 5.7% of birds, 2.7% of mammals, and 1.3% of reptiles. These findings underscore the potential role of these diverse species as reservoirs or incidental hosts in the transmission cycle of IAV, emphasizing the need for expanded research on less-studied taxa and their ecological interactions. The results also contribute to our understanding of the epidemiology of IAV in the Neotropics and can inform future surveillance and mitigation strategies. Full article

DNA metabarcoding is a powerful biodiversity monitoring tool, enabling simultaneous assessments of diverse biological communities. However, its accuracy depends on the reliability of reference databases that assign taxonomic identities to obtained sequences. Here we provide a DNA barcode dataset for aquatic fauna of the Panama Canal, a region that connects the Western Atlantic and Eastern Pacific oceans. This unique setting creates opportunities for trans-oceanic dispersal while acting as a modern physical dispersal barrier for some terrestrial organisms. We sequenced 852 specimens from a diverse array of taxa (e.g., fishes, zooplankton, mollusks, arthropods, reptiles, birds, and mammals) using COI, and in some cases, 12S and 16S barcodes. These data were collected for a variety of studies, many of which have sought to understand recent changes in aquatic communities in the Panama Canal. The DNA barcodes presented here are all from captured specimens, which confirms their presence in Panama and, in many cases, inside the Panama Canal. Both native and introduced taxa are included. This dataset represents a valuable resource for environmental DNA (eDNA) work in the Panama Canal region and across the Neotropics aimed at monitoring ecosystem health, tracking non-native and potentially invasive species, and understanding the ecology and distribution of these freshwater and euryhaline taxa. Full article

Tick-borne piroplasmids are apicomplexan protozoa that infect a wide range of vertebrate hosts, with significant implications for animal and human health. This study investigated the occurrence and genetic diversity of piroplasmids in wild mammals, unconventional pets, and associated ticks in Goiás state, midwestern Brazil. Between April 2023 and January 2024, 105 blood samples, 22 tissue samples, and 300 ticks were collected from 21 mammalian species housed in wildlife screening centers, zoos, and veterinary clinics. Molecular screening targeting the 18S rRNA gene of piroplasmids detected a 25.7% (27/105) overall positivity, with gray brockets (Subulo gouazoubira) and South American tapirs (Tapirus terrestris) showing the highest infection rates. Three tick samples tested positive, including two Amblyomma sculptum nymphs and a male of Amblyomma dubitatum collected from a tapir and capybara (Hydrochoerus hydrochaeris). Cytauxzoon brasiliensis was reported, for the first time, in cougars (Puma concolor) from Goiás state, midwestern Brazil, indicating the role of this feline as a host of this parasite. Babesia goianiaensis was confirmed in a capybara, and Theileria terrestris in tapirs. Phylogenetic analyses clustered gray brockets-associated Theileria sequences with Theileria sp. previously detected in Neotropical deer from Brazil and Theileria cervi. While the phylogenetic analysis of amino acid sequences of the cytochrome c oxidase subunit III separated Theileria genotypes detected in S. gouazoubira from T. cervi, hsp70-based phylogenetic inferences clustered the genotypes detected in Tapirus terrestris with Theileria terrestris, suggesting host-specific evolutionary lineages. These findings contribute to the understanding of Piroplasmida diversity and circulation in South American wild mammals, emphasizing the need for enhanced molecular surveillance to elucidate transmission dynamics, assess potential health risks, and contribute to the establishment of wildlife conservation and One Health strategies. Full article

We describe a new genus and species of the tribe Ichthyomyini (Rodentia: Sigmodontinae) based on three specimens collected in Machupicchu, Cusco, in the southern Peruvian Andes. Our study includes a comprehensive morphological analysis of 201 specimens representing all recognized species, employing multivariate statistics (principal component analysis) of external and cranial measurements, as well as phylogenetic methods. We used maximum parsimony for morphological data and concatenated molecular datasets (Cytochrome b [17 species], IRBP [15 species], and RAG1 [11 species]) analyzed via maximum likelihood and Bayesian inference. The new genus and species exhibit an allopatric distribution relative to other Ichthyomyini and are distinguished by the following combination of traits: dull slate-gray dorsal fur, lighter ventrally without contrast to the dorsum; incomplete philtrum; vestigial pinna concealed within head fur; long, broad hindfeet with a well-developed fringe of stiff hairs and brown soles; laterally compressed tail exceeding head-body length; type 1 carotid circulation pattern; absence of the orbicular apophysis of the malleus; presence of posteroloph and posterolophid in M1, M2, m1, and m2; unilocular hemiglandular stomach (non-reduced). Full article

The genus Leptospira includes at least 69 Gram-negative, aerobic spirochetes, of which 25 are pathogenic and associated with a diverse range of mammals, including members of the order Chiroptera. On the American continent, there are six confirmed Leptospira species. Among these, the common vampire bat (Desmodus rotundus), which ranges widely from northern Mexico to northern Argentina, has been reported to harbor four pathogenic taxa: Leptospira borgpetersenii, Leptospira interrogans, Leptospira weilii, and Leptospira cf. noguchii. All these species are frequently isolated from beef and dairy cattle, suggesting that contact with urine from infected cattle could serve as a potential source of infection for bats. However, previous studies have been limited by small sample sizes and low geographical representation among the countries where they were conducted. For this reason, the aim of this study was to identify the species of Leptospira associated with D. rotundus populations in five states within the Neotropical region of Mexico. Between 2015 and 2021, 54 bats were collected across five Mexican states. Our analysis identified the exclusive presence of L. interrogans in 13 specimens. The findings are discussed within the framework of a One Health perspective, emphasizing their relevance to understanding interspecies transmission dynamics. Full article

Conservation strategies that use sustainable use of natural resources through green-labelled markets generally do not recognize the legal sale of wild meat as appropriate due to potential overexploitation and zoonotic disease risks. Wildlife hunting is important to the livelihoods of rural communities living in tropical forests for protein and income. Wildlife management plans in the Peruvian Amazon permit hunting of wild meat species for subsistence and sale at sustainable levels, that include peccaries, deer, and large rodents. These species have fast reproduction making them less vulnerable to overhunting than other species. This study assessed the sustainability of a wildlife management plan. Populations of species were estimated using camera traps and distance transect surveys, and sustainability analysis used hunting pressure from community hunting registers. Interviews were conducted to understand hunters, perceptions of the management plan. Long-term time-series showed increases in collared peccary (3.0 individual/km² to 5.41 individual/km²) and white-lipped peccary (3.50 individual/km² to 7.00 individual/km²) populations and short-term time series showed a decline in paca populations from 8.5 individual/km² to 3.01 individual/km². The unified harvest analysis showed permitted species populations were greater than 60% of their carrying capacities and hunted at less than 40% of their production, which shows sustainable hunting. The wildlife management plan achieved its general objective of sustainable hunting and improving livelihoods. The broader question is whether sustainable wildlife use plans that allow Amazonian communities to sell limited amounts of wild meat can be a way to change illegal wild meat trade to a legal, green labelled trade with added value. Full article

In the Neotropical biogeographic region, there are about 290 known species and subspecies of fleas, which have as their main hosts to the rodent subfamily Sigmodontinae. In Peru, more than 22 years have passed since the last list of known flea species was published. This study presents a review and update of the fleas recorded in Peru from 1908 to 2024. This list contains information on the flea species, including their known hosts, the localities in Peru where they were reported, the main references and some comments. In addition, the specificity of flea–host interactions was assessed using an interaction network approach. In the literature review, 86 flea species were found distributed among 9 families, 34 genera and 19 subspecies. These fleas were recorded parasitizing at least71 species of mammals and birds (14 orders, 28 families, 54 genera), 15 reported only at the genera level and seven undetermined ones. The flea–host interaction network exhibited a modular structure, indicating that these interactions tend to be specialized. A sigmodontine rodent (Akodon mollis) and a Rhopalopsyllidae flea (Polygenis litargus) were highly connected across modules and the overall network, and thus were important to maintain the structure. This review contributes to the knowledge of fleas in Peru, synthesizing the scattered information and providing information on the interaction they have with their hosts. Full article

Large wild mammals are extremely important in their respective ecological communities and are frequently considered to be emblematic. This is the case of the different tapir species, the largest terrestrial mammals from the Neotropics. Despite their large size and being objects of interest for many naturalists, the field still lacks critical genetics and systematics information about tapir species. In the current work, we analyzed four molecular datasets (mitogenomes, and three nuclear genes, RAG 1-2, IRBP, and BRCA1) of two South American tapirs: the Andean tapir (Tapirus pinchaque) and the alleged new species of tapir, Tapirus kabomani. We derived four main findings. (1) Our molecular phylogenetic analyses showed T. pinchaque as the youngest tapir branch in Neotropics and a sister species of Tapirus terrestris. This contradicts the traditional morphological observations of renowned zoologists and paleontologists, who considered T. pinchaque as the oldest Neotropical tapir. (2) Our data does not support that the alleged T. kabomani is a full species. Rather, it is a specific group within T. terrestris. (3) T. pinchaque is the Neotropical tapir species which yielded the lowest levels of genetic diversity (both for mitochondrial and nuclear data). (4) The spatial genetic structure for T. pinchaque shows differences depending on the type of molecular marker used. With mitogenomes, the spatial structure is relatively weak, whereas with two nuclear genes (RAG 1-2 and IRBP), the spatial structure is highly significant. Curiously, for the other nuclear gene (BRCA1), the spatial structure is practically nonexistent. In any case, the northernmost population of T. pinchaque we studied (Los Nevados National Park in Colombia) was in a peripatric situation and was the most genetically differentiated. This is important for the adequate conservation of this population. (5) T. pinchaque showed clear evidence of population expansion during the last part of the Pleistocene, a period during which the dryness and glacial cold extinguished many large mammals in the Americas. However, T. pinchaque survived and spread throughout the Northern Andes. Full article

Habitat loss and fragmentation are pervasive processes driving the disappearance of populations and species in the Neotropical region. Since species loss may translate into functional loss, assessing changes in the composition of assemblages’ functional traits might improve our understanding of the ecological roles played by species and ecosystem functioning. Here, we investigate how landscape structure and composition impact the functional diversity of terrestrial mammals in 18 forest patches composing eight protected areas in Southern Brazil. We used functional diversity (FD) based on dietary, physical, and behavioral traits and species vulnerability to extinction. We determined which landscape variables (patch size, proportions of forest and sugarcane, and patch isolation) most influenced mammal FD values by using a both-direction stepwise model selection from a linear global model. Finally, we evaluated the role of trophic guilds in explaining the variation in the FD values using a Principal Component Analysis. Between 2012 and 2017, using camera traps, we recorded 26 native medium- and large-sized mammals throughout the protected areas, of which 6 are regionally threatened, and 5 domestic/exotic species. Richness among the forest patches varied from 4 to 24 species (9.05 ± 5.83), while the FD values varied from 1.29 to 6.59 (2.62 ± 1.51). FD variation was best explained by patch size, which exhibited a strong positive correlation (adjusted R² = 0.55, slope = 0.67, p < 0.001). Insectivores and frugivores presented the highest correlation with patch size, explaining most of the variation in the FD values. Our findings strengthen the paramount role of large protected areas in maintaining mammal diversity and their ecological functions in human-modified landscapes. Full article

Orchid seeds are predominantly wind-dispersed, often developed within dry, dehiscent fruits that typically release millions of dust-like seeds into the air. Animal-mediated seed dispersal is a lesser-known phenomenon in the family and predominantly occurs in groups belonging to early-diverging lineages bearing indehiscent, fleshy fruits with hard, rounded, dark seeds. In this review, we explore the evolutionary trends of seed dispersal mechanisms in Orchidaceae, focusing on the pantropical genus Vanilla. Notably, certain Neotropical species of Vanilla produce vanillin-aromatic compounds synthesized naturally in their fruits, which plays a pivotal role in seed dispersal. Ectozoochory occurs in dry, dehiscent fruits, whose seeds are dispersed by (i) male euglossine bees collecting the fruit’s vanillin aromatic compounds and (ii) female stingless bees collecting the fruit’s mesocarp. Endozoochory occurs in (iii) highly nutritious, indehiscent fruits consumed by terrestrial mammals or (iv) fleshy, dehiscent fruits whose mesocarp is consumed by arboreal mammals. Wind dispersal appears to be a derived state in Orchidaceae and, given its predominance, a trait likely associated with enhanced speciation rates. Zoochory primarily occurs in groups derived from early-diverging lineages; occasional reversions suggest a link between dispersal mode and fruit and seed traits. Interestingly, fruit dehiscence and fleshiness in Vanilla lack phylogenetic signal despite their role in determining dispersal modes, suggesting potential environmental adaptability. Full article

The latest reorganization of the Vertebrate collections preserved at the “Pietro Doderlein” Museum of Zoology of the University of Palermo (Italy) has made it possible to draw up a check-list of the Mammal taxa present in the stuffed (M), fluid-preserved (ML) and anatomical (AN) collections. The intervention was planned under the National Biodiversity Future Center (NBFC) agenda, focused on the enhancement of Italian natural history museums. The growing interest in museum collections strongly demands databases available to the academic and policy world. In this paper, we record 679 specimens belonging to 157 specific taxa arranged in 58 families and 16 orders. Most of the species (75.1%) come from the Palaearctic Region (southern Mediterranean and North Africa), with a minority of taxa coming from the Afrotropical (7.8%), Neotropical (4.6%), Indo-Malayan (3.4%) and Australasian (1%) regions. Among the 24% of the taxa listed in the IUCN categories as threatened (VU, EN, CR, RE) the specimens of the Sicilian wolf, a regional endemic subspecies that became extinct in the last century, stand out. Even if small (<1000 specimens), the collection of mammals of the Museum of Zoology is an important asset for research on biodiversity in the Mediterranean area, representing an international reference for those wishing to conduct morphological and genetic studies in this area. Full article

Fire occurrence affects the distribution of key resources for fauna in natural ecosystems worldwide. For fire management strategies adequate for biodiversity conservation, the understanding of how species respond to fire-induced changes is essential. In this study, we investigated the role of fire regimes on spaces used by medium and large mammals at multiple spatial scales (0.8 ha to 78.5 ha) in a fire-prone savanna ecosystem (Brazilian Cerrado). We sampled mammals using 60 camera traps distributed in 30 sampling units located in grassland and typical savanna formations. We applied single-species occupancy models and AIC-based model selection to assess how mammals use the space in response to pyrodiversity (both diversity of fire frequencies and diversity of fire ages), the proportion of recently burned area, and the proportion of long-unburned area while accounting for detectability. Our results showed that fire regime variables affected the study species differently. Deer species used the space regardless of mosaic pyrodiversity and the proportion of specific fire ages. Fire-related variables, however, affected space use by tapirs and maned wolves. Tapirs preferred to use fire mosaics with lower diversity of fire frequencies, whereas maned wolves more intensively used mosaics with high fire age diversity and a high proportion of recently burned areas. Based on our findings, we recommend that fire management targeting specific mammal species should not necessarily focus on maximum pyrodiversity. Instead, we suggest a management strategy combining “patch mosaic burning” with the maintenance of specific fire-age patches suitable for different species’ requirements. Full article

Arthropod-borne viruses (arboviruses) are a diverse group of ribonucleic acid (RNA) viruses, with the exception of African swine fever virus, that are transmitted by hematophagous arthropods to a vertebrate host. They are the important cause of many diseases due to their ability to spread in different environments and their diversity of vectors. Currently, there is no information on the geographical distribution of the diseases because the routes of transmission and the mammals (wild or domestic) that act as potential hosts are poorly documented or unknown. We conducted a systematic review from 1967 to 2021 to identify the diversity of arboviruses, the areas, and taxonomic groups that have been monitored, the prevalence of positive records, and the associated risk factors. We identified forty-three arboviruses in nine mammalian orders distributed in eleven countries. In Brazil, the order primates harbor the highest number of arbovirus records. The three most recorded arboviruses were Venezuelan equine encephalitis, Saint Louis encephalitis and West Nile virus. Serum is the most used sample to obtain arbovirus records. Deforestation is identified as the main risk factor for arbovirus transmission between different species and environments (an odds ratio of 1.46 with a 95% confidence interval: 1.34–1.59). The results show an increase in the sampling effort over the years in the neotropical region. Despite the importance of arboviruses for public health, little is known about the interaction of arboviruses, their hosts, and vectors, as some countries and mammalian orders have not yet been monitored. Long-term and constant monitoring allows focusing research on the analysis of the interrelationships and characteristics of each component animal, human, and their environment to understand the dynamics of the diseases and guide epidemiological surveillance and vector control programs. The biodiversity of the Neotropics should be considered to support epidemiological monitoring strategies. Full article

The Neotropical underground rodents of the genus Ctenomys (Rodentia: Ctenomyidae) comprise about 65 species, which harbor the most significant chromosomal variation among mammals (2n = 10 to 2n = 70). Among them, C. minutus stands out with 45 different cytotypes already identified, among which, seven parental ones, named A to G, are parapatrically distributed in the coastal plains of Southern Brazil. Looking for possible causes that led to such extensive karyotype diversification, we performed chromosomal mapping of different repetitive DNAs, including microsatellites and long interspersed element-1 (LINE-1) retrotransposons in the seven parental cytotypes. Although microsatellites were found mainly in the centromeric and telomeric regions of the chromosomes, different patterns occur for each cytotype, thus revealing specific features. Likewise, the LINE-1-like retrotransposons also showed a differential distribution for each cytotype, which may be linked to stochastic loss of LINE-1 in some populations. Here, microsatellite motifs (A)₃₀, (C)₃₀, (CA)₁₅, (CAC)₁₀, (CAG)₁₀, (CGG)₁₀, (GA)₁₅, and (GAG)₁₀ could be mapped to fusion of chromosomes 20/17, fission and inversion in the short arm of chromosome 2, fusion of chromosomes 23/19, and different combinations of centric and tandem fusions of chromosomes 22/24/16. These data provide evidence for a correlation between repetitive genomic content and localization of evolutionary breakpoints and highlight their direct impact in promoting chromosomal rearrangements. Full article

(1) Background: Southern Amazonia encompasses some of the most human-impacted and deforested regions of South America, resulting in a hyper-fragmented landscape. In this context, by using a geometric morphometrics approach, we aimed to examine the effect of forest fragmentation on developmental instability (DI) of the mandible, assessed by variation of fluctuating asymmetry (FA), in four neotropical small mammal species inhabiting the municipality of Alta Floresta (Brazil). (2) Methods: The impact of fragment area, fragment shape, isolation, and edge length on DI were assessed by measuring variation in mandibular FA in the long-tailed spiny rat (Proechimys longicaudatus), the hairy-tailed bolo mouse (Necromys lasiurus), the woolly mouse opossum (Marmosa demerarae), and the Amazonian red-sided opossum (Monodelphis glirina). Mandibles from a total of 304 specimens originating from different-sized fragments (ranging from 5 to 900 ha) were used. Twelve homologous landmarks were digitized in photographs of the mesial view of each hemi-mandible. (3) Results: The two largest species, P. longicaudatus and M. demerarae, exhibited significantly higher levels of FA in mandibular shape in small fragments (5–26 ha) in comparison to large ones (189–900 ha). Edge length negatively impacted M. demerarae, the only arboreal species, reinforcing its strongest dependence on core forest habitats. (4) Conclusions: For small mammal communities, we propose that fragments >~200 ha should be the focus of conservation efforts, as both resilient and more sensitive species would benefit from their more preserved biotic and abiotic conditions. Conversely, fragments <~25 ha seem to lead to a significant increase in stress during developmental stages. Full article