Search Results (58)

Curbside saturation in dense commercial corridors compromises the sustainability of last mile logistics. This study investigates the impact of “authorized but non functional occupancy” (Class S (Service)), referring to service and tradespeople vehicles, on the operational capacity of loading and unloading zones (LUZ). Based on direct field observations of 474 real vehicle entries in a zone in Zaragoza (Spain), an Erlang B no wait queuing model (M/M/1/1) using weighted occupancy time was applied to contrast current saturation levels with a regulated functional scenario. The results demonstrate that the infrastructure is structurally sufficient: removing inefficient uses reduces traffic intensity from 1.31 to 0.48 Erlangs, increasing service potential by 121.84%. Class S was identified as consuming 36.62% of the lost capacity, exceeding the impact of unauthorized private cars. Total Hidden Carbon Emissions (HCE) amounted to 45.34 kg CO₂, establishing an environmental impact of 0.066 kg CO₂ per misused linear meter. The study concludes that proper utilization of loading zones is sufficient to accommodate logistics demand and effectively reduce CO₂ emissions. Full article

Bayberry (Morella rubra Lour.; syn. Myrica rubra (Lour.) Siebold & Zucc.) leaves are rich in bioactive compounds but remain underutilized. This study investigated the optimal harvest stage and processing methods to develop high-quality functional powder. We first compared three growth stages: red buds (RB), new leaves (NL), and old leaves (OL). RB exhibited the highest antioxidant capacity and unique volatile profile; however, NL was selected for processing optimization due to the balance between quality and biomass availability. Subsequently, NL was subjected to freeze-drying (FD), mechanical drying (MD), steaming followed by MD (S-MD), and shade drying (SD). Results showed that FD preserved the vibrant green color, glandular trichome structure, ascorbic acid, and fresh volatiles (monoterpenes). Conversely, thermal drying (MD and S-MD) disrupted cellular barriers, which facilitated the extraction of minerals and robust polyphenols like myricitrin, yielding the highest extraction of flavonoids and corresponding antioxidant activity, measured by hydrophilic oxygen radical absorbance capacity (H-ORAC), in hot water extracts than FD. SD significantly degraded quality due to prolonged enzymatic oxidation. While FD is ideal for preserving aesthetics and heat-sensitive nutrients, low-cost MD and S-MD are recommended for producing antioxidant-rich powders for functional food applications where extraction efficiency is prioritized. Full article

Background: Rehabilitation of the severely atrophic maxilla remains a major challenge in implant dentistry, particularly when conventional endosseous implants and regenerative procedures are contraindicated due to extensive bone loss, sinus pathology, or patient-related factors. Advances in digital planning and additive manufacturing have enabled the reintroduction of juxta-osseous subperiosteal implants as a graftless, patient-specific treatment option. This case report aimed to describe the complete digital workflow, surgical placement, and immediate prosthetic rehabilitation of a customized juxta-osseous subperiosteal implant in a patient with severe posterior maxillary atrophy and a history of failed sinus augmentation procedures. Case Presentation: A 75-year-old male patient presenting with left severe posterior maxillary atrophy and previous unsuccessful sinus lift surgeries was rehabilitated using a digitally designed, additively manufactured titanium subperiosteal implant. Cone-beam computed tomography–based planning and CAD–CAM technology were used to design a patient-specific framework, which was rigidly fixed to stable maxillofacial support and immediately loaded with a screw-retained provisional prosthesis. Results: Clinical and radiographic follow-up demonstrated stable implant fixation, soft tissue healing, absence of biological or mechanical complications, and satisfactory functional and aesthetic outcomes. The patient reported high levels of comfort and satisfaction throughout the treatment period. Conclusions: Digitally manufactured juxta-osseous subperiosteal implants may represent a predictable and minimally invasive graftless alternative for selected patients with severe maxillary atrophy, particularly when conventional implant placement or extensive bone augmentation is not feasible. Accurate digital planning, rigid fixation, and appropriate patient selection appear to be key factors for clinical success. Full article

Urban curbside loading and unloading zones are increasingly affected by competing non-logistics uses, such as outdoor terraces or resident parking, leading to reductions in effective curbside length. These design decisions can significantly alter service capacity and generate environmental externalities in urban freight operations that are rarely quantified. This study introduces the Factor of Occupancy (F_o) as a space–time design indicator for curbside unloading zones, defined as the product of effective curbside length and the maximum authorised dwell time. Using direct observational data from an urban block in Zaragoza (Spain), the analysis focuses on a loading and unloading zone whose effective length was reduced by approximately 6 m due to the installation of a restaurant terrace. Two curbside configurations are compared: a reduced configuration (8 m) and a restored configuration (14 m), keeping demand and temporal constraints constant. F_o is integrated into a loss-based queueing model (M/M/1/1) to estimate blocking probabilities and the number of served and rejected freight operations. To capture the environmental implications of curbside capacity loss, the paper proposes the Hidden Carbon Emissions (HCE) indicator, which quantifies the additional CO₂ emissions generated by rejected vehicles through block recirculation and idling during illegal occupancy, based on observed behaviour and publicly available emission factors. The results show that restoring curbside length substantially increases effective service capacity and reduces rejected vehicles, leading to a marked decrease in hidden CO₂ emissions per operation. The findings highlight that minor curbside design decisions can produce measurable impacts on both urban freight efficiency and environmental performance. Full article

This study analyses the operational efficiency of urban loading and unloading zones (LUZs) by applying queuing theory without waiting (Erlang B model) and incorporating weighted occupancy time as a fundamental metric. Six scenarios were evaluated in an urban block in Zaragoza, Spain: three using field data obtained through real world observation and three simulated. The system’s performance was compared under conditions of free access with a model that strictly enforces the municipal ordinance for Urban Goods Distribution, restricting access to authorized vehicles and maximum dwell times. The objective of this study is to evaluate the operational performance of different LUZ configurations, assessing how real versus regulation-compliant usage affects system capacity, estimated loss rates, and the spatial temporal productivity of the zones. The M/M/1/1 model in Kendall notation is suitable for representing this type of queuing-free urban environment, and weighted occupancy time proves to be a robust indicator for evaluating the performance of heterogeneous zones. The scenario assessment confirms that the sizing of these zones is correct if their proper use is guaranteed. The study concludes with recommendations and best practices for city governance in formulating urban policies aimed at developing more efficient and sustainable logistics to control land use in the LUZ. Full article

Sugar transporters of the SWEET family play a crucial role in sugar partitioning and fruit quality, yet their functions remain uncharacterized in Chinese bayberry (Morella rubra). In this study, we present the first genome-wide identification and characterization of the SWEET gene family in species, revealing 15 MrSWEET genes distributed across eight chromosomes. Phylogenetic analysis classified these genes into four conserved clades with distinct tissue-specific expression patterns. During fruit ripening, transcripts of MrSWEET1, 2b, 4, and 15 accumulated progressively, with MrSWEET15 showing the strongest increase. Weighted gene co-expression network analysis (WGCNA) identified a “lightcyan” module that correlates strongly with fruit sugar content (r > 0.8) and contains MrSWEET15, three additional MrSWEET genes, and 47 transcription factors (Dof, MYB, NAC, ERF, MADS, WRKY). Promoter analysis of MrSWEET15 revealed the presence of light- and hormone-responsive cis-elements (MYB, MYC, HY5, bZIP, and Dof), and MYB1, HY5, and Dof1.5 expression profiles are synchronized with MrSWEET15, suggesting potential regulatory relationships. These findings establish MrSWEET15 as a priority candidate for sugar transport in Chinese bayberry and our understanding of the molecular basis of sugar transport and fruit quality formation. Full article

Immunosenescence, defined as the progressive decline of immune function with age, is increasingly recognized as a determinant of morbidity in people living with HIV (PLWH) and in individuals with metabolic dysfunction. The coexistence of chronic viral infection and systemic metabolic alterations—including metabolic dysfunction-associated steatotic liver disease (MASLD) and type 2 diabetes mellitus—creates a pro-inflammatory state (“metaflammation”) that accelerates immune aging. This narrative review synthesizes current clinical, translational, and experimental evidence on the cellular, molecular, and metabolic mechanisms underlying immunosenescence in HIV-positive and HIV-negative populations with metabolic dysfunction. Key converging pathways include chronic inflammation, mitochondrial dysfunction, microbial translocation, and altered immunometabolic signaling, leading to features such as CD8⁺CD28⁻ T-cell expansion, reduced CD4/CD8 ratios, and impaired vaccine responses. Biomarkers such as iAge, IMM-AGE, and the triglyceride–glucose (TyG) index have emerged as promising tools to quantify immune aging beyond chronological measures. Understanding these interconnected mechanisms offers opportunities for targeted interventions—such as metabolic reprogramming, microbiota modulation, and geroscience-based strategies—aimed at preserving immune resilience and promoting healthy aging in high-risk populations. Full article

Background: Wedge-shaped implants have been proposed as a minimally invasive solution for narrow alveolar ridges, aiming to avoid bone augmentation. While the short-term results are promising, long-term clinical evidence remains limited. Methods: This multicenter prospective single-arm cohort study reports the 7-year outcomes of tissue-level wedge-shaped implants (1.8 mm thickness) placed without grafting in horizontally atrophic ridges (mean thickness 3.73 ± 0.36 mm). Clinical and radiographic evaluations were performed on 45 implants (34 patients). Results: At the 7-year post-loading follow-up, the implant survival rate was 95.5%, with two failures recorded—one early loss and one due to peri-implantitis. Peri-implant mucositis was observed in 5 implants (11.4%), while peri-implantitis was diagnosed in 3 implants (6.8%). No mechanical complications were reported. The mean marginal bone loss (MBL) was 1.45 ± 1.41 mm, measured relative to the implant shoulder. Multivariate linear regression identified older age (β = +0.040; p = 0.012) and mandibular implant placement (β = +1.39; p = 0.007) as significant predictors of greater bone loss. Conclusions: Wedge-shaped implants demonstrated high long-term survival and stable marginal bone levels in narrow ridges without the need for bone augmentation. Age and mandibular location negatively influenced long-term bone stability, while smoking, gender, and history of periodontitis were not significant predictors. Full article

The use of LUZs in urban environments is a critical factor for ensuring efficient vehicle mobility in cities. Poor utilisation of these zones can generate negative externalities, such as double parking or illegal occupation of pedestrian crossings or garage doors. The purpose of the study is to provide city governance with a methodology based on the OEE model to evaluate the efficiency of individual zones or sets of zones and to inform decisions that improve their use without disrupting the coexistence with other city users. To validate the methodology, all deliveries made in selected areas of the city of Zaragoza over the course of one month were studied. The results of the study reveal a considerable loss of efficiency and some recommendations are proposed achieve a better use: only 51.44% of deliveries used the LUZs correctly, and the total OEE ratio was just 0.37. This low level of efficiency is due to the incorrect use by delivery drivers, who often use LUZs as parking spaces, and the illegal occupation of the zones by unauthorised private vehicles. Full article

Desulfovibrio vulgaris is an anaerobic microorganism belonging to the group of sulphate-reducing bacteria (SRB). SRB form biofilms on metal surfaces in water supply networks, producing a microbiologically influenced corrosion (MIC). This process produces the deterioration of metal surfaces, leading to high economic costs and different environmental safety and health problems related to its chemical treatment. For that reason, rapid and accurate detection methods of SRB are needed. In this work, a new detection system for Desulfovibrio has been developed using gated nanoporous materials. The probe is based on hybrid nanoporous alumina films encapsulating a fluorescent molecule (rhodamine B), whose release is controlled by an oligonucleotide gate. Upon exposure to Desulfovibrio’s genomic material, a movement of the oligonucleotide gatekeeper happens, resulting in the selective delivery of the entrapped rhodamine B. The developed material shows high selectivity and sensitivity for detecting Desulfovibrio DNA in aqueous buffer and biological media. The implementation of this technology for the detection of Desulfovibrio as a tool for monitoring water supply networks is innovative and allows real-time in situ monitoring, making it possible to detect the growth of Desulfovibrio inside of pipes at an early stage and perform timely interventions to reverse it. Full article

The study of woody dicot xylem structure in relation to habitats has a long but geographically incomplete history, generating generalizations and questions still in need of expanded data. One understudied area is localized cross-habitat studies under identical climate conditions. Also sparse are intraspecific cross-habitat data. Both of these weaknesses are addressed in the present project for unstudied S. Florida. Six surveys of woody dicot branchlet microtome cross-sections allowed the microscopic comparison of vessel element (VE) diameters and VE densities. The project took place in a small area within short timeframes per survey to assure near uniformity in weather and in seasonal growth cycles. The multispecies Initial Survey and single-species Ximenia americana Survey addressed the question of adjustments in VE diameters and/or VE densities in shaded vs. sunny habitats, finding significant downward average adjustment in VE densities in shaded hammock vs. open pineland habitats (112 shade vs. 182 VE/mm² sun) but not in VE diameters. Single-species (Chrysobalanus icaco, Morella cerifera) surveys examined adjustments in VE diameters and VE densities in swamp vs. scrub (diameter mean: 42.5 µm swamp, 49.2 µm scrub; density means: 179.9 swamp, 154.0 scrub). Chrysobalanus icaco, having arguably the greatest environmental breadth and having the largest mean vessel element diameters in the project, was the sole species to adjust VE diameters only across habitats. Coefficients of variation in VE density exceeded those in VE diameters in every survey. This project sets the stage for future work in Florida and beyond aimed at isolation of environmental variables with respect to xylem traits and aimed at causal mechanisms, especially mode of xylem adjustment in relation to conductive risk. Full article

Chinese bayberry (Myrica rubra or Morella rubra) is a valuable fruit, yet the mechanism of its flesh segment development is not well understood. Using paraffin sectioning, we investigated the flower buds of the ‘Biqi’ and ‘Zaojia’ varieties, revealing that the flesh segment development in these Chinese bayberry varieties involved the formation of a primordium outside the ovary wall, the establishment of a simple columnar structure, and the formation of the primary flesh segment. Assessment of endogenous hormone levels indicated the significant reductions in jasmonic acid (JA) and indole-3-acetic acid (IAA) levels at the critical stages of flesh segment development. Correlation analysis highlighted the essential roles of IAA, JA, abscisic acid (ABA), and gibberellins in the flesh segment developmental process, underscoring the complex interactions driven primarily by the IAA, JA, and ABA networks. Gene modules positively correlated with flesh segment development were identified using transcriptome-based weighted gene co-expression network analysis (WGCNA). Differentially expressed genes (DEGs) were enriched in plant hormone signal transduction pathways, particularly for upregulated genes associated with auxin and JA signaling. Key genes predicted to be involved in flesh segment development included LAX2 and LAX3 (auxin transport), JAZ6 (JA signaling repression), and KAN1 and KAN4 (regulating multiple hormonal signaling pathways). Quantitative real-time polymerase chain reaction (qRT-PCR) validation confirmed that the expression trends for these genes were consistent across both varieties, particularly for CRC, SEP1, SEP3, IAA7, and JAZ6. Immunofluorescence localization studies revealed that auxin was primarily distributed in the central vascular bundle and outer cells of the flesh segment. This uneven auxin distribution might contribute to the unique morphology of flesh segments. Overall, this study provides insights into the hormonal regulation and genetic factors involved in the development of Chinese bayberry flesh segments. Full article

Wastewater-based epidemiology has emerged as a complementary tool for the monitoring of COVID-19 pandemic waves and for the circulation of viral variants. The selection, standardization, and dynamics of different SARS-CoV-2 RNA targets in wastewater requires further investigation. In the present study, 106 wastewater samples were collected over a 24-month period from the wastewater treatment plant of Sondrio, north Italy, and were analyzed for the presence of SARS-CoV-2 RNA through the quantification of ORF1b, N1, and N3 gene targets via one-step real-time qPCR. In general, the three RNA targets demonstrated different performances and dynamics over the studied time period, underlying the usefulness of multiple viral targets in the surveillance of SARS-CoV-2 in wastewater. During the first 12 months, the quantification of the selected SARS-CoV-2 viral targets also correlated with the reported clinical cases in the same geographical area; however, from the overall data analysis this did not appear to significantly anticipate the epidemic waves. In conclusion, this study further supports the use of wastewater surveillance as a real time indicator of the human circulation of SARS-CoV-2. Moreover, the use of multiple viral gene targets has been shown to improve the reliability of SARS-CoV-2 surveillance in wastewater over time. Full article

Deletion and duplication in the human 16p11.2 chromosomal region are closely linked to neurodevelopmental disorders, specifically autism spectrum disorder. Data from neuroimaging studies suggest white matter microstructure aberrations across these conditions. In 16p11.2 deletion and duplication carriers, potential gene dosage effects may impact white matter organisation, contributing to phenotypes including impaired cognition. However, the biological mechanisms underlying this white matter pathology remain unclear. To bridge this knowledge gap, we utilised mouse models of 16p11.2 deletion and duplication to explore changes in corpus callosum oligodendrocytes, myelination, axon caliber, and astrocytes. Immunofluorescence staining was employed to measure lineage and mature oligodendrocyte numbers, as well as myelin basic protein and glial fibrillary acidic protein fluorescence intensity. Transmission electron microscopy was utilised to evaluate axonal structural alterations related to myelin, such as myelinated axon percentage, diameter, myelin thickness, and g-ratio. Our findings reveal changes in the number of mature oligodendrocytes, myelination levels, axon diameter, and astrocytes in the corpus callosum of mice with 16p11.2 deletion and duplication. Deletion mice displayed a tendency toward reduced counts of mature oligodendrocytes and myelination levels, while duplication mice exhibited a notable increase. Axon diameter variations included a significant increase in axon diameter and myelin thickness in both deletion and duplication mice, but with irregular structure in duplication mice. Variances in astrocytes between genotypes showed significant early increases in development for both deletion and duplication mice compared to wild-type mice, with this rise sustained in duplication mice but significantly diminished in deletion mice at a later stage. Our research reveals changes in the biological mechanisms impacting white matter. Comparison of reciprocal trends in 16p11.2 deletion and duplication mice with wild-type mice suggests the possibility of gene dosage effects. Identification of these mechanisms offers an initial step in unveiling therapeutic targets for associated neurodevelopmental disorder phenotypes. Full article

In this article, we propose the detection of crowd anomalies through the extraction of information in the form of time series in video format using a multimodal approach. Through pattern recognition algorithms and segmentation, informative measures of the number of people and image occupancy are extracted at regular intervals, which are then analyzed to obtain trends and anomalous behaviors. Specifically, through temporal decomposition and residual analysis, intervals or specific situations of unusual behaviors are identified, which can be used in decision-making and the improvement of actions in sectors related to human movement such as tourism or security. This methodology introduces a novel, privacy-focused approach by analyzing anonymized metrics rather than tracking or recognizing individuals, setting a new standard for ethical crowd monitoring. Applied to the webcam of Turisme Comunitat Valenciana in the town of Morella (Comunitat Valenciana, Spain), this approach has shown excellent results, correctly detecting specific anomalous situations and unusual overall increases during the previous weekend and during the October 2023 festivities. These results have been obtained while preserving the confidentiality of individuals at all times by using measures that maximize anonymity, without trajectory recording or person recognition. Full article