Search Results (44)

Forest mushrooms, due to their taste and smell, have been a component of people’s diets since the beginning of time. Unfortunately, there are many inedible or poisonous species of mushrooms that are similar to those that are eaten. For example, the highly valued Boletus edulis is similar to the inedible bitter bolete and the poisonous bolete. In the case of mushrooms of the genus Lactarius, such similarities are demonstrated by the delicious tasting L. deliciosus, the inedible downy L. pubescens and the poisonous cottony L. torminosus. This study presents an attempt to classify these three species based on studies of the emission of volatile organic compounds from the volatile headspace using proton-transfer reaction mass spectrometry (PTR-MS). The conducted statistical tests, principal component analysis (PCA) and discriminant analysis revealed significant differences in the concentration of 20 selected protonated VOC molecules for the tested mushroom species. The clear advantages of the PTR-MS technique are that there is no need for special sample preparation and it has rapid measurement capability and high analytical sensitivity. This allows for a quick comparative analysis of VOCs, for example, from different species of forest mushrooms. Full article

Lactarius hatsudake is an economically important wild edible fungus in sub-tropical and temperate Asia. At present, little is known about its genetic diversity, mode of reproduction, and population structure in natural environments. In this study, we collected 102 specimens from eight geographic locations across three provinces in southwest China. Five simple sequence repeat markers that demonstrated high polymorphism were developed and used to analyze the patterns of genetic variations within and among the eight geographic populations. Analyses of molecular variance revealed that 60% of the observed genetic variation occurred among populations, with the remaining 40% attributable to within populations, while inter-provincial variation was nonsignificant. Combined analyses based on discriminant analysis of principal components, STRUCTURE, and the unweighted pair group method with arithmetic mean identified two distinct genetic subpopulations with each genetic subpopulation showing a wide geographical distribution, a result consistent with ancient divergence and recent gene flow within this species in southwest China. Interestingly, excess homozygosity was observed at most loci in almost all of the eight geographic populations, consistent with inbreeding being common for this species in nature. Together, our results revealed the genetic diversity, mode of reproduction, and geographic structuring of this important wild mushroom in southwest China. Full article

Lactarius deliciosus is an edible, seasonal, wild-growing forest mushroom with significant functional properties and potential applications in health-promoting foods. The aim of the study was to compare the level of selected functional compounds (minerals, phenols, indoles, L-phenylalanine, lovastatin, ergothioneine, glucans, chitin, chitosan) and Lactic Acid Bacteria (LAB) in freeze-dried snacks made from the fermented caps of L. deliciosus mushrooms. The snacks were made from mushrooms blanched in water or microwave, and fermentation was carried out using one of the strains of probiotic bacteria: L. acidophilus (LA-5) or L. plantarum (SWA016). After 6 months of storage, mushroom products were a good source of functional compounds, especially LAB, minerals, indoles, lovastatin, antioxidants (phenolic compounds), and dietary fibre. Fermentation with added probiotic cultures enhanced indigenous lactobacilli levels, but after storage, only microwave-blanched snacks fermented with L. plantarum retained a high LAB count (7.3 log CFU/g). The selection of pre-treatment significantly influenced bioactive compound composition: water blanching enhanced lovastatin and 6-methyl-D,L-tryptophan contents, whereas microwave blanching maximised K, S, Rb, Fe, Se, Mn, Br, phenolic compounds, antioxidant activity, and soluble dietary fibre. In order to optimise the level of the most important bioactive compounds and LAB, microwave blanching with the addition of L. plantarum SWA016 should applied. Full article

Owing to their nutritional, culinary, and nutraceutical, mushrooms are worldwide consumed and appreciated. Moreover, many of these mushrooms are also known as medicinal mushrooms since they possess several pharmacological properties attributable to a huge number of bioactive compounds derived from their sporophores. Several studies are available in the literature about in vitro and in vivo mechanisms of actions of such bioactive compounds. Most of these surveys are focused on macrofungi belonging to the genera Pleurotus, Ganoderma, or specific taxa such as Agaricus bisporus, Agaricus blazei, Boletus eduliInonotus obliquus, Hericium erinaceus, Lentinula edodes, and Grifola frondose. On the other hand, there is a lack of information on the under investigated ecological group of freshwater fungi. These fungi play a very important role in freshwater environments and some of them, belonging to Basidiomycota, are also edible and largely consumed. In this review we collected information about the medicinal properties of freshwater macro- and micromycetes. Among them, macrofungi, such as Amanita vaginata, Armillaria mellea, Armillaria tabescens, Astraeus hygrometricus, Auricularia auricula-judae, Bjerkandera adusta, Bovista nigrescens, Calocybe gambosa, Candolleomyces candolleanus, Collybia dryophila, Coprinus comatus, Cyclocybe cylindracea, Hypsizygus ulmarius, Inonotus hispidus, Lactarius controversus, Lentinus tigrinus, and Schizophyllum commune, observed in riparian habitat, and microfungi, such as Penicillium aculeatum, P. chrysogenum, and Fusarium incarnatum, isolated from aquatic plants, have been reported to have antimicrobial, anticancer, anti-inflammatory, antioxidant, antidiabetic, immunomoludatory, hypoglycaemic, and other pharmaceutical activities. Such fungal species are noteworthy since they represent an important quote of biodiversity to preserve their fundamental ecological role and a possible solution for different health problems for humans and animal farms. Full article

This study focuses on the classification of six different macrofungi species using advanced deep learning techniques. Fungi species, such as Amanita pantherina, Boletus edulis, Cantharellus cibarius, Lactarius deliciosus, Pleurotus ostreatus and Tricholoma terreum were chosen based on their ecological importance and distinct morphological characteristics. The research employed 5 different machine learning techniques and 12 deep learning models, including DenseNet121, MobileNetV2, ConvNeXt, EfficientNet, and swin transformers, to evaluate their performance in identifying fungi from images. The DenseNet121 model demonstrated the highest accuracy (92%) and AUC score (95%), making it the most effective in distinguishing between species. The study also revealed that transformer-based models, particularly the swin transformer, were less effective, suggesting room for improvement in their application to this task. Further advancements in macrofungi classification could be achieved by expanding datasets, incorporating additional data types such as biochemical, electron microscopy, and RNA/DNA sequences, and using ensemble methods to enhance model performance. The findings contribute valuable insights into both the use of deep learning for biodiversity research and the ecological conservation of macrofungi species. Full article

Bisphenol A is a plastic component, which shows endocrine activity that is detrimental to humans and aquatic ecosystems. The elimination of BPA from the environment is one of the solutions for BPA contaminant management. Adsorption is a cost-effective, easy-to-use method generating low harmful byproducts; nevertheless, contaminant sorbent treatment is a challenge that still needs to be addressed. Fungal fruiting bodies biomass is rarely studied sorbent but is promising due to its high polysaccharide content and availability. Our preliminary studies showed BPA sorption (100 mg/L) by 50 cultivated and wild fungi. The cultivated species: Clitocybe maxima (82%), Pholiota nameko (77%), and Pleurotus columbinus (74%), and wild fungi Cantharellus cibarius (75%) and Lactarius deliciosus (72%) were the most efficient. The biomass was able to sorb BPA over a broad range of temperature and pH levels, with an optimum at 20 °C and pH 7. Although saturation of sorbents was rapid, the regeneration process using ethanol was effective and allowed to recover up to 75% of sorbents’ initial efficiency. A single use of 1 g of sorbent would allow the treatment of 8.86 to 10.1 m³ of wastewater effluent, 16.5 to 18.7 m³ of surface water, and 411 to 469 m³ of drinking water, assuming the concentrations of BPA reported in the literature. Full article

Slavonia is the most developed agricultural region in Croatia. With rich and fertile soils that have enabled the cultivation of a wide variety of fruits, vegetables, and cereals, Slavonia has always met the food needs of its population. Today, the biocultural diversity of local varieties and semi-natural vegetation has irretrievably disappeared. Our aim was to document the remaining local knowledge of plant use in this area through in-depth semi-structured interviews, which were conducted in 2022–2023. All possible aspects of the use of plants and fungi were recorded as food, animal feed, medicine, construction, jewelry, rituals and ceremonies, dyes, etc. The names and uses of local plant varieties were also recorded. The results show 1702 entries—a total of 296 plant taxa from 76 families and 28 fungi from 16 families. The most frequently named plants were: Urtica dioica, Robinia pseudoacacia, Rosa canina, and Sambucus nigra. The plants with the greatest variety of uses were Morus alba, Rosmarinus officinalis, Triticum aestivum, and Zea mays. Interesting uses were identified. The leaves of the ornamental plant Hosta sieboldiana are still used today as food for wrapping meat with rice, the aquatic plant Trapa natans is eaten like chestnuts, and Pteridium aquilinum was once consumed as a vegetable. In addition, Ambrosia artemisiifolia and Sambucus ebulus were given to horses to prevent and avoid blood poisoning. Some forest species had a special significance and were revered or favored. The most frequently mentioned edible fungi were Boletus sp., Cantharellus cibarius, and Lactarius piperatus. Auricularia auricula-judae is the only species stated to have been used exclusively as a raw snack. Evidence of edible use of Sarccoscypha coccinea, which was reported as traditionally consumed in the past, was of particular interest. Despite the modernization and agricultural nature of the region, many interesting uses of plants and fungi were identified. Further efforts should be directed towards documenting this knowledge to facilitate its dissemination in the communities that possess it, or at least to preserve it for future generations. Full article

Lactarius hatsudake Tanaka is a mycorrhizal edible mushroom with an appealing taste and rich nutrition. It is also a significant food and has medicinal value. In this study, the plantation of L. hatsudake during the harvest period was taken as the research object, and this article explores which bacteria in the soil contribute to the production and growth of L. hatsudake. The soil of the control (CK) and the soil of the mushroom-producing area [including the soil of the base of the mushroom (JT) and the mycorrhizal root soil (JG)] was collected in the plantation. The three sites’ bacterial community structure and soil diversity were analyzed using high-throughput sequencing technology, and a molecular ecological network was built. Soil bacteria in the L. hatsudake plantation had 28 tribes, 74 classes, 161 orders, 264 families, 498 genera, and 546 species. The dominant phyla were Proteobacteria and Acidobacteria, and the dominant genera were Burkholderia_Caballeronia_Paraburkholderia, Acidothermus, Bradyrhizobium, Candidatus_Xiphinematobacter, and Granulicella. The α-diversity of soil bacteria in JT was significantly lower than that in JG and CK, and the β-diversity in JT samples was significantly different from that in JG and CK samples. The size and complexity of the constructed network were smaller in JT samples than in JG and CK samples, and the stability was higher in JT samples than in JG and CK samples. The positive correlation between species in JT samples was dominant. The potential mycorrhizal helper bacteria (MHB) species of L. hatsudake was determined using correlation and differential group analysis. The results support future research on mycorrhizal synthesis, plantation management, and the function of microorganisms in the soil rhizosphere of L. hatsudake. Full article

The integral role of wild fungi in ecosystems, including provisioning, regulating, cultural, and supporting services, is well recognized. However, quantifying and predicting wild mushroom yields is challenging due to spatial and temporal variability. In Mediterranean forests, climate-change-induced droughts further impact mushroom production. Fungal fruiting is influenced by factors such as climate, soil, topography, and forest structure. This study aims to quantify and predict the mycological potential of Lactarius deliciosus in sustainably managed Mediterranean pine forests using machine learning models. We utilize a long-term dataset of Lactarius deliciosus yields from 17 Pinus pinaster plots in Soria, Spain, integrating forest-derived structural data, NASA Landsat mission vegetation indices, and climatic data. The resulting multisource database facilitates the creation of a two-stage ‘mycological exploitability’ index, crucial for incorporating anticipated mycological production into sustainable forest management, in line with what is usually done for other uses such as timber or game. Various Machine Learning (ML) techniques, such as classification trees, random forest, linear and radial support vector machine, and neural networks, were employed to construct models for classification and prediction. The sample was always divided into training and validation sets (70-30%), while the differences were found in terms of Overall Accuracy (OA). Neural networks, incorporating critical variables like climatic data (precipitation in January and humidity in November), remote sensing indices (Enhanced Vegetation Index, Green Normalization Difference Vegetation Index), and structural forest variables (mean height, site index and basal area), produced the most accurate and unbiased models (OA_training = 0.8398; OA_validation = 0.7190). This research emphasizes the importance of considering a diverse array of ecosystem variables for quantifying wild mushroom yields and underscores the pivotal role of Artificial Intelligence (AI) tools and remotely sensed observations in modeling non-wood forest products. Integrating such models into sustainable forest management plans is crucial for recognizing the ecosystem services provided by them. Full article

Mushrooms contain phenolic compounds that possess health-promoting properties, including antioxidant effects. However, the low solubility and form of phenolic compounds affect their bioactivity and bioaccessibility. To overcome this limitation, our study investigates the fermentation of mushrooms to increase their free phenolic content and enhance their bioactivity. Our research focused on the impact of fermentation on both free and bound phenolic fractions (FPs and BPs, respectively) in Lentinula edodes and Lactarius deliciosus, which were successively fermented with Lactiplantibacillus plantarum LMG 17673 for 72 h. We examined the total phenolic content (TPC), phenolic profile, and antioxidant activity of both FPs and BPs. Our results showed that the TPC of BPs was higher than that of FPs in both mushrooms, with strong antioxidant capabilities. Fermentation significantly increased the TPC of FPs in both mushrooms, particularly after 24 h of fermentation. The TPC of BPs in mushrooms decreased during fermentation, indicating their release from the matrix. Additionally, we identified 30 bioactive compounds using UPLC-Q-TOF-MS/MS. Our study demonstrates for the first time that lactic acid bacteria fermentation of mushrooms with high phenolic content leads to the liberation of bound phenolics, enhancing their bioactivity and bioaccessibility. Full article

Lactarius hatsudake is a common ectomycorrhizal edible mushroom in Pinus massoniana forests, and has important ecological and potential economic values. However, there are only a few reports on the establishment of Pinus massoniana–Lactarius hatsudake symbiosis. Here, we isolated a new strain of L. Lactarius hatsudake (GX01) from a local masson pine forest and established its ectomycorrhizal symbiosis with the P. massoniana. Potato dextrose agar (PDA) medium was optimal for the growth of L. hatsudake GX01. The saffron-to-brown ectomycorrhiza formed by L. hatsudake GX01 are usually bifurcated or coralloid shape, with a rod and a smooth surface, without emanating hyphae. The characteristic mantle and Hartig net structures of ectomycorrhizae were confirmed by microscope and scanning electron microscope (SEM). L. hatsudake GX01 can significantly promote the formation and development of lateral roots of P. massoniana seedlings during the early interaction. This study thus lays the foundation for subsequent study of the symbiotic molecular mechanism and application of P. massoniana–L. hatsudake symbiosis. Full article

Lactarius hatsudake (LH), a great wild endemic fungus, contains rich nutritional components with medicinal properties. The effects of LH on body weight, liver weight, liver injury, blood lipids, and gut microbiota in C57BL/6 mice fed a high-fat diet (HFD) for 8 weeks was examined in this research. Though there was no clear impact on weight loss, the findings indicate that LH treatment effectively decreased liver damage caused by HFD, as well as lowered serum total cholesterol, triacylglycerol, and low-density lipoprotein cholesterol levels. Additionally, it positively influenced gut microbiota to resemble that of mice on a normal diet. In HFD-fed mice, LH markedly boosted the levels of Parabacteroides, unclassified Muribaculaceae, Oscillibacter, and unclassified Oscillospiraceae, while reducing the abundance of Lachnospiraceae NK4A136 group and Erysipelatoclostridium, as well as the ratio of Firmicutes to Bacteroidetes. Further analysis of correlation indicate a possible connection between obesity and gut microbiota. Obesity-related indices show a positive association with unclassified Eubacterium coprostanoligenes group, Blautia, and Erysipelatoclostridium, while displaying a negative correlation with unclassified Muribaculaceae, unclassified Clostridia vadinBB60 group, Helicobacter, Oscillibacter, unclassified Ruminococcaceae, Parabacteroides, and unclassified Oscillospiraceae. The results suggest that LH can help combat obesity and may have the potential to be utilized as a functional food. Full article

Herein, we present an integrative investigation of the nutritional and nutraceutical potential of Lactarius indigo, Clitocybe nuda, Clitocybe subclavipes, Russula delica, Russula brevipes, Clitocybe squamulosa, and Amanita jacksonii, which are edible mushrooms consumed in the northeastern highlands of Puebla, Mexico. The content of protein oscillated from 4.8 to 10.9 g 100 g⁻¹ fresh weight (FW) whereas that of fiber ranged from 8.8 to 19.7 g 100 g⁻¹ FW. The edible species presented low amounts of fat (1.5–3.4 g 100 g⁻¹ FW) and reducing sugars (0.8–2.9 g 100 g⁻¹ FW), whereas the content of vitamin C oscillated from 6.5 to 84.8 mg 100 g⁻¹ dry weight (DW). In addition, four vitamins of B complex (thiamine, riboflavin, vitamin B₆, and folate) were determined in different concentrations. A high abundance of potassium (92.3–294.3 mg 100 g⁻¹ DW), calcium (139.1–446.9 mg 100 g⁻¹ DW), and magnesium (81.3–339.1 mg 100 g⁻¹ DW) was determined in most of the edible mushrooms, as well as detectable levels of p-hydroxybenzoic acid (2.2–48.7 mg 100 g⁻¹ DW), protocatechuic acid (0.5–50.8 mg 100 g⁻¹ DW), oleic acid (14.2–98.3 mg 100 g⁻¹ DW), linoleic acid (748–1549.6 mg 100 g⁻¹ DW), and linolenic acid (from 9.1 to 83.6 mg 100 g⁻¹ DW). The total phenol content and antioxidant capacity significantly (p < 0.05) varied among the studied species, and their capacity to inhibit enzymes involved in glucose, lipid, and polyamine metabolism. Nevertheless, the hydroalcoholic extracts from A. jacksonii and L. indigo efficiently inhibited alpha-glucosidase and ornithine decarboxylase (IC₅₀ < 50 µg mL⁻¹), respectively. The evaluation of the same extracts on microorganisms associated with the gastrointestinal tract showed negligible toxicity on probiotics (MIC > 500 µg mL⁻¹) and moderate toxicity against pathogenic bacteria (MIC < 400 µg mL⁻¹). Based on the studied parameters, principal component analysis and orthogonal partial least squares discriminant analysis clustered these edible mushrooms into two main groups with similar biological or chemical properties. Full article

Tree species composition in forest ecosystems is an important biological factor affecting the diversity of ectomycorrhizal fungi (EMF). However, little is known about the composition and diversity of EMF communities associated with Pinus massoniana in different P. massoniana association habitats (MpAHs) in subtropical mountains. This study investigated the EMF community characteristics of P. massoniana in different MpAHs using plant community surveys, soil property analyses, and mycorrhizal identification. A total of 56 operational taxonomic units (OTUs), belonging to 20 families and 22 genera, were identified. OTU richness of Basidiomycota (58.93%) was higher than that of Ascomycota (41.07%). Unclassified Helotiales, Russula, Lactarius, and Tomentella were the dominant groups. Different stand types significantly altered the EMF communities of P. massoniana (p < 0.05, for Shannon index) and the associations of P. massoniana + Populus adenopoda (Mp_Pa) had the highest diversity of EMF, while P. massoniana + Cunninghamia lanceolata (Mp_Cl) had the lowest diversity. The number of specific OTUs was higher than shared OTUs. Similarity index and principal coordinate analysis indicated that the EMF communities of P. massoniana varied significantly in different MpAHs (R² = 0.21, p = 0.001). The linear regression model showed that the EMF diversity of P. massoniana was positively related to tree species diversity, indicating that the EMF diversity of P. massoniana is influenced by tree species diversity. The findings provide a reasonable reference for tree species configuration in the process of mixed transformation or near-natural management of plantations. Full article

The characterization of natural fungal diversity impacts our understanding of ecological and evolutionary processes and can lead to novel bioproduct discovery. Russula and Lactarius, both in the order Russulales, represent two large genera of ectomycorrhizal fungi that include edible as well as toxic varieties. Based on morphological and phylogenetic analyses, including nucleotide sequences of the internal transcribed spacer (ITS), the 28S large subunit of ribosomal RNA (LSU), the second largest subunit of RNA polymerase II (RPB2), the ribosomal mitochondrial small subunit (mtSSU), and the translation elongation factor 1-α (TEF1-α) gene sequences, we here describe and illustrate two new species of Russula and one new species of Lactarius from southern China. These three new species are: R. junzifengensis (R. subsect. Virescentinae), R. zonatus (R. subsect. Crassotunicatae), and L. jianyangensis (L. subsect. Zonarii). Full article