Search Results (87)

Pathogens that have developed resistance to antibiotics pose a threat to public health. The primary goal in preventing foodborne infections is to inhibit the growth of and, subsequently, eliminate antibiotic-resistant pathogens at every stage from production to consumption. Escherichia coli, which has acquired resistance to most known antibiotics, is frequently found in chicken meat. In many countries, due to unregulated antibiotic use in poultry farming, poor hygiene in slaughterhouses, or cross-contamination, extended-spectrum beta-lactamase (ESBL)-producing E. coli has been identified as the causative agent in poultry-associated food poisoning. The need for more effective antimicrobial agents against this pathogen, which is resistant to existing antibiotics, has led to increased attention being paid to postbiotics produced by lactic acid bacteria, particularly bacteriocins. This study aimed to determine the antimicrobial effects of postbiotics obtained from kefir-derived Lactiplantibacillus plantarum and Lactococcus lactis against ESBL-positive E. coli. To achieve this, E. coli strains were isolated from raw chicken meat samples collected from the market using culture-based methods, and their antimicrobial resistance profiles were determined using the disk diffusion method. The ESBL positivity of the isolates was assessed using the double-disk synergy test. The antimicrobial activities of the postbiotics against the identified ESBL-positive E. coli strains were tested using the macro-dilution method to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. ESBL-positive E. coli was detected in 48% of raw chicken meat samples. The antimicrobial effects of postbiotics were examined by disk diffusion, and postbiotics produced by 18 Lb. plantarum strains and 20 Lc. lactis strains showed strong antimicrobial activity. Significant differences in the antimicrobial effects of postbiotics were observed between the two species. Lb. plantarum postbiotics exhibited both bacteriostatic (concentration 60%) and bactericidal (concentration 80%) effects on ESBL-positive E. coli strains, whereas Lc. lactis postbiotics showed only bacteriostatic effects (80% concentration). Postbiotics derived from probiotic bacteria offer promising effects against multidrug-resistant E. coli due to their heat resistance, activity across different pH values, strong antimicrobial effects, affordability, and ease of production. Full article

Background: Fermentation is among the oldest and most versatile food processing techniques, enhancing not only shelf life but also nutritional and functional value. While Asian and Western fermented foods are extensively studied, traditional Eastern European fermentations—such as sauerkraut, kefir, bryndza, kvass, and sourdough—remain largely unexplored despite their enduring cultural and dietary importance. These foods combine spontaneous or mixed-culture fermentations, diverse substrates, and unique microbial consortia that may yield distinct bioactive profiles with potential health benefits. Methods: This narrative review synthesizes data from scientific articles, regional reports, and ethnographic sources retrieved from PubMed, Scopus, and Google Scholar up to 2025. Studies were selected for relevance to composition, microbiology, bioactive compounds, and human or experimental health outcomes related to Eastern European fermented foods. Results: Available evidence indicates that traditional fermented dairy, cereal, and vegetable products from Eastern Europe contain fermentation-derived bioactive compounds, including specific bioactive peptides, transformed polyphenols, microbial-synthesized vitamins, organic acids, and live or non-viable microorganisms. Experimental studies describe the generation of ACE-inhibitory peptides, polyphenol biotransformation, and prebiotic or postbiotic metabolites with reported antioxidant, antihypertensive, immunomodulatory, and metabolic effects. However, substantial variability in artisanal production practices and the limited number of standardized human studies currently constrain definitive conclusions. Conclusions: Eastern European fermented foods represent a culturally unique yet scientifically undercharacterized component of functional nutrition. Their complex microbial ecosystems and diverse substrates offer valuable models for studying diet–microbe interactions. Further omics-based and clinical research is warranted to clarify bioavailability, mechanisms of action, and their potential integration into evidence-based dietary strategies. Full article

Antimicrobial resistance (AMR) remains a critical global challenge, requiring novel complementary strategies beyond antibiotic development. Probiotic-fermented foods (PFFs) offer an emerging, low-cost approach to mitigate AMR risk through ecological, molecular, and immunological mechanisms. This review integrates mechanistic insights, clinical evidence, and translational frameworks linking PFFs to antimicrobial stewardship. Key mechanisms include colonization resistance, nutrient and adhesion-site competition, production of antimicrobial metabolites, such as bacteriocins, hydrogen peroxide, and organic acids and Quorum-quenching-sensing activities that suppress pathogen virulence. Randomized clinical trials indicate that fermented diets and probiotic supplementation can improve microbiome diversity, reduce inflammatory cytokines, and decrease antibiotic-associated diarrhea, though direct AMR outcomes remain underexplored. Evidence from kefir, kombucha, and other microbial consortia suggests potential for in vivo pathogen suppression and reduced infection duration. However, safe translation requires standardized starter-culture genomics, resistome monitoring, and regulatory oversight under QPS/GRAS frameworks. Integrating PFF research with One Health surveillance systems, such as the WHO GLASS platform, will enable tracking of antimicrobial consumption and resistance outcomes. Collectively, these findings position PFFs as promising adjuncts for AMR mitigation, linking sustainable food biotechnology with microbiome-based health and global stewardship policies. Full article

Developing the nutritional profile of food through the fermentation produced by lactic acid bacteria is part of sustainable development, but it also helps to improve public health. Thus, the objective of the study was to obtain synbiotic juices of fruit origin, which can successfully substitute well-known probiotic dairy products and also be introduced into the diet of vegetarians or people with certain intolerances to animal products. In this regard, two fermented blueberry juices were obtained: NC, using kefir cultures, and NP with Lactobacillus plantarum inoculation. Two more simple blueberry juices were used as controls: NCM, thermostatted at 24 °C and NPM, at 37 °C. The four types of juices were subjected to physicochemical and microbiological analyses. The results show that NP juice is the best substitute from a physicochemical point of view, presenting the highest polyphenol content and the highest DPPH radical scavenging activity even after refrigeration for 5 days. Additionally, the microbiological results of the analyzed juices can recommend these products for industrial production. Full article

Dairy products harbor complex and dynamic microbial communities that contribute to their sensory properties, safety, and cultural distinctiveness. Raw milk contains a diverse microbiota shaped by seasonality, storage conditions, lactation stage, animal health, farm management, and genetics, serving as a variable starting point for further processing. Fermentation, whether spontaneous or starter driven, selects for subsets of lactic acid bacteria (LAB), yeasts, and molds, resulting in microbial succession that underpins both artisanal and industrial products such as kefir and cheese. Kefir represents a balanced LAB–yeast symbiosis, with species composition influenced by grain origin, milk type, and processing parameters, whereas the cheese microbiota reflects the interplay of starter and non-starter LAB, coagulants, ripening conditions, and “house microbiota”. Methodological factors—including DNA extraction, sequencing platform, and bioinformatic pipelines—further impact the reported microbial profiles, highlighting the need for standardization across studies. This review synthesizes current knowledge on raw milk, kefir, and cheese microbiomes, emphasizing the biological, technological, environmental, and methodological factors shaping microbial diversity. A holistic understanding of these drivers is essential to preserve product authenticity, ensure safety, and harness microbial resources for innovation in dairy biotechnology. Full article

Kefir is a historic dairy-fermented beverage produced using lactic acid bacteria and yeast as a starter culture and is considered nutritious with a good taste. Many studies have been conducted to incorporate various possible functional materials into kefir to enhance its nutritional value. This study aims to enrich kefir with 0.25% and 0.5% of Stinging nettle (Sn) powder before fermentation to improve its nutritional value. Stinging nettle (Urtica dioica) is a nutritious and multifunctional herb with a variety of healthful components such as fibers and polyphenols; it has significant potential as a useful food functional ingredient. The physicochemical, microbial, and nutritional properties of kefir fortified with Sn were examined weekly during refrigerated storage for 21 days. The results showed that adding Stinging nettle significantly (p < 0.05) increased the probiotic counts from 7.90 ± 0.22 log to 8.46 ± 0.19 log CFU/g, antioxidant activity (4%), and total polyphenol contents (5%) in kefir yogurt after 12 days of refrigerated storage. The addition of Sn also had a positive effect on the acidity of kefir and increased the viscosity and the syneresis to a certain extent. Furthermore, adding Sn increased lactic acid bacteria counts and the production of short-chain fatty acids after in vitro digestion and colonic fermentation. The results of this study indicated the potential use of Sn powder as a functional ingredient in kefir yogurt and other similar products. Full article

Indigenous Mexican fermented beverages, such as pulque, colonche, tepache, and water kefir, are pillars of the country’s cultural and gastronomic heritage. Their sensory attributes and health-promoting properties arise from complex microbial consortia, in which lactic acid bacteria (LAB), mainly Lactobacillus and Leuconostoc, acetic acid bacteria (AAB), primarily Acetobacter, and yeasts such as Saccharomyces and Candida interact and secrete exopolysaccharides (EPSs). Dextran, levan, and heteropolysaccharides rich in glucose, galactose, and rhamnose have been consistently isolated from these beverages. EPSs produced by LAB enhance the viscosity and mouthfeel, extend the shelf life, and exhibit prebiotic, antioxidant, and immunomodulatory activities that support gut and immune health. Beyond food, certain EPSs promote plant growth, function as biocontrol agents against phytopathogens, and facilitate biofilm-based bioremediation, underscoring their biotechnological potential. This review integrates recent advances in the composition, biosynthetic pathways, and functional properties of microbial EPSs from Mexican fermented beverages. We compare reported titers, outline key enzymes, including dextransucrase, levansucrase, and glycosyltransferases, and examine how fermentation variables (the substrate, pH, and temperature) influence the polymer yield and structure. Finally, we highlight emerging applications that position these naturally occurring biopolymers as sustainable ingredients for food and agricultural innovation. Full article

Certain probiotic strains have been proposed to alleviate mental health conditions, such as anxiety and stress, by modulating the gut–microbiota–brain axis through the production of metabolites like gamma-aminobutyric acid (GABA). This study evaluated kefir-derived microbial strains for their GABA-producing capacity in mono- and co-culture systems using whey as the growth substrate. Based on the screening results, two microbial consortia were selected to develop fermented whey beverages with raspberry (FWF-R1 and FWF-R2). These beverages were characterized for their technological and functional properties over 21 days of refrigerated storage and following gastrointestinal digestion. Both formulations maintained stable acidity and showed a slight increase in viscosity during storage. The microbial counts remained above 8.5 log colony-forming units/mL, with high post-digestion viability, confirming their probiotic potential. The GABA levels increased progressively during storage, reaching 2.67 mM in FWF-R1 and 4.65 mM in FWF-R2, with recovery rates of 40–45% after digestion. The total phenolic content decreased moderately during storage but increased ~5-fold after digestion; the total anthocyanins declined by up to 70%. FWF-R2 achieved higher sensory acceptability and was preferred by 58% of consumers, emerging as the most promising formulation. These findings highlight the psychobiotic potential of these beverages and support the sustainable valorization of dairy and fruit by-products. Full article

Background/Objectives: Lactobacillus kefiranofaciens HL1, isolated from kefir, exhibits antioxidant and anti-aging activities, defined here as improved cognitive function and reductions in oxidative stress and inflammatory markers. However, its poor milk viability limits application. This study developed a novel fermented milk by co-culturing HL1 with Lactococcus lactis subsp. cremoris APL015 (APL15) to enhance fermentation and health benefits. Methods: HL1 and APL15 were co-cultured to produce fermented milk (FM), and fermentation performance, microbial viability, texture, and syneresis were evaluated. A D-galactose-induced aging BALB/c mouse model was used to assess cognitive function, oxidative stress, inflammation, antioxidant enzyme activity, and gut microbiota after 8 weeks of oral administration. Results: FM reached pH 4.6 within 16 h, with high viable counts (~10⁹ CFU/mL) for both strains. HL1 viability and texture were maintained, with smooth consistency and low syneresis. In vivo, FM improved cognitive behavior (Y-maze, Morris water maze), reduced oxidative damage (MDA), lowered IL-1β and TNF-α, and enhanced brain SOD levels. FM-fed mice exhibited increased short-chain fatty acid producers, higher cecal butyrate, and reduced Clostridium perfringens. Conclusions: The co-cultured fermented milk effectively delivers HL1 and provides antioxidant, anti-inflammatory, and anti-aging effects in vivo, likely via gut–brain axis modulation. It shows promise as a functional food for healthy aging. Full article

This study analyzes the aromatic profiles of kiwi-based fermented beverages, inoculated with varying proportions of milk kefir grains and incubated under different shaking rates. The experiments were designed using response surface methodology and three consecutive batch cultures were performed under each experimental condition. At the end of each fermentation, the grains were separated from the beverage and reused as the inoculum for fermenting fresh kiwi juice in the subsequent batch. Based on the results, together with the previously determined microbiological and chemical characteristics, two beverages were identified as having broader aromatic profiles, lower contents of sugars, ethanol, and acids, and high counts of lactic acid bacteria (LAB) and yeasts (>10⁶ CFU/mL). These beverages were produced under relatively low agitation rates (38 and 86 rpm) and high inoculum proportions (4.33% and 4.68% w/v) during the second and third batch cultures, respectively. Over 28 days of refrigerated storage, the pH values of both beverages remained relatively stable, and the LAB counts consistently exceeded 10⁶ CFU/mL. Yeast counts, along with the production of ethanol, glycerol, lactic acid, and acetic acid, increased slightly over time. In contrast, the concentrations of citric acid, quinic acid, total sugars, and acetic acid bacteria declined by day 28. Full article

Dairy products—encompassing yogurt, kefir, cheese, and cultured milk beverages—are emerging as versatile, food-based modulators of gut microbiota and host physiology. This review synthesizes mechanistic insights demonstrating how live starter cultures and their fermentation-derived metabolites (short-chain fatty acids, bioactive peptides, and exopolysaccharides) act synergistically to enhance microbial diversity, reinforce epithelial barrier integrity via upregulation of tight-junction proteins, and modulate immune signaling. Clinical evidence supports significant improvements in metabolic parameters (fasting glucose, lipid profiles, blood pressure) and reductions in systemic inflammation across metabolic syndrome, hypertension, and IBS cohorts. We highlight critical modulatory factors—including strain specificity, host enterotypes and FUT2 genotype, fermentation parameters, and matrix composition—that govern probiotic engraftment, postbiotic yield, and therapeutic efficacy. Despite promising short-term outcomes, current studies are limited by heterogeneous designs and brief intervention periods, underscoring the need for long-term, adaptive trials and integrative multi-omics to establish durability and causality. Looking forward, precision nutrition frameworks that harness baseline microbiota profiling, host genetics, and data-driven fermentation design will enable bespoke fermented dairy formulations, transforming these traditional foods into next-generation functional matrices for targeted prevention and management of metabolic, inflammatory, and neuroimmune disorders. Full article

This study aimed to identify kiwi kefir-like beverages with high levels of viable probiotic cells and low levels of calories, acids, and alcohol. To achieve this, microbiological and chemical characterizations were conducted on beverages inoculated with varying amounts of kefir grains (GW) and incubated at different agitation speeds (A), following a second-order orthogonal factorial design. For each experimental condition, three 24-h batch cultures were performed using three successive passages of kefir grains. Higher GW levels promoted greater nutrient consumption and metabolite production. However, an intermediate GW (1.80 g) resulted in the highest growth of lactic acid bacteria (LAB), acetic acid bacteria (AAB), yeasts, and free biomass in the fermented medium. Optimal agitation levels also enhanced nutrient consumption, free biomass, and metabolite pro-duction. AAB and yeast counts increased with higher agitation speeds, while LAB counts de-creased. Three beverages, produced during the second (A = 86 rpm, GW = 2.81 g) and third (A = 38 rpm, GW = 2.60 g; A = 86 rpm, GW = 1.80 g) kefir grain passages, exhibited LAB and yeast counts above 10⁶; CFU/mL, along with low total sugar and ethanol concentrations. These beverages may be considered suitable as potentially probiotic, low-alcohol, and low-calorie functional drinks. Full article

In a global context marked by food insecurity and the increasing prevalence of non-communicable diseases, this study proposes a healthy food basket (HFB) model tailored to the demographic, cultural, and economic specificities of the Republic of Moldova which is aligned with international standards. The research employed a comprehensive methodology, including estimations of daily energy requirements using revised Harris–Benedict equations, food selection based on nutritional value, economic availability, and cultural relevance, and nutritional validation through the mean adequacy ratio (MAR), which was derived from nutrient adequacy ratios (NARs) and dietary reference values (DRVs) established by the EFSA. Nutrient intake calculations were based on food composition data and not population-level dietary surveys. Fat-soluble vitamins were excluded due to insufficient available data. The results indicate adequate intake levels of vitamins (B₁, B₂, B₃, and C) and minerals (iron, magnesium, phosphorus, and potassium) while highlighting deficiencies in calcium and sodium that require dietary adjustments. The inclusion of traditional foods, such as kefir and salted or raw pork fat, underscores the model’s cultural acceptability and economic relevance, strengthening the integration of global nutritional principles with regional dietary habits. This study’s limitations, including the use of secondary data and the lack of empirical validation, highlight the need for longitudinal studies. The HFB model offers a replicable solution for other regions facing similar challenges, contributing to global efforts to reduce malnutrition and promote sustainable diets. Full article

The aim of this work was to evaluate the impact of fortifying goat milk kefir with high-value ingredients (3% and 6% date paste, and 25% and 50% goat milk substitution with date–cheese whey), derived from the valorization of date coproducts, on its nutritional (proximate composition and mineral profile), technological (pH, acidity, viscosity, color, sugar and organic acid content), microbiological and sensory properties. Both ingredients enhanced the growth and stability of the kefir starter culture, thereby improving the probiotic potential of date-added kefir and also its nutritious quality (lower fat content and higher protein content). The mineral profile of kefir was improved only when the date paste was added. Date paste could be used as an ingredient in fortified kefir (up to 6%) without altering its flow properties because it was perfectly integrated within the milk matrix. The use of date–cheese whey as a goat milk substitution (>25%) decreased the typical kefir viscosity, inducing an excessive phase separation negatively valued by consumers. Consumers preferred the kefir with 6% date paste mainly due to its higher scores for aroma, flavor, sweetness and acidity. Full article

The rising interest in functional fermented beverages, such as kombucha and water kefir, has stimulated research into their health benefits. This study aimed to investigate the combined bioactive potential of kombucha and water kefir by fermenting a novel medium prepared by mixing them in a 1:1 v/v ratio. The fermentation process involved using both SCOBY and water kefir grains (WKGs) separately, as well as co-cultivation, to explore the bioactive properties of the three fermented beverages. Samples were analyzed at 24, 48, and 72 h for changes in pH, microorganism growth, and concentrations of flavonoids and phenolics. Antioxidant activity was assessed using DPPH, ABTS, and FRAP tests, alongside colorimetric analysis and enzyme inhibition assays against α-amylase, α-glucosidase, and lipase. The results demonstrated that longer fermentation times increased both bioactive compound content and antioxidant capacity. The highest phenolic concentration was found in the WKG-fermented mixture (47.58 ± 2.13 mg GAE/100 mL), while the highest iron-reducing capacity was observed in the product fermented with both WKGs and the co-culture of SCOBY-WKGs. Additionally, SCOBY fermentation showed significant inhibitory activity (over 70%) against digestive enzymes. These findings suggest that co-fermenting kombucha and water kefir represents a promising alternative to traditional water kefir, with improved bioactive compound profiles. Full article