Search Results (26)

Functional ingredients from Jerusalem artichoke (JA) were produced using a 2² experimental design with two factors: “pretreatment” (W: water immersion; P: pressing with citric acid dip) and “drying method” (A: air-drying; F: freeze-drying). Four powders (JAPWA, JAPPA, JAPWF, and JAPPF) were analyzed for technological properties, inulin, phenolic, and flavonoid content, in vitro prebiotic activity score (PAS), and antioxidant capacity. Pretreatment influenced inulin content. JAPPF exhibited the highest PAS value (1.12 ± 0.08), whereas JAPWA presented the lowest (0.58 ± 0.04). These differences could be attributed to the influence of polyphenol content, as the freeze-dried powders retained more than twice the concentration due to the effect of low process temperatures, which usually protect phenolic compounds. Using a more cost-effective alternative, JAPPA exhibited better technological properties as well as higher inulin content and PAS than JAPWA. Full article

Mid-season drought is increasingly recognized as a major constraint on tuber production in Jerusalem artichoke. The ability of different genotypes to maintain high yields under such conditions is a critical component of drought tolerance. This study aimed to investigate the effects of mid-season drought on tuber yield, biomass, harvest index, tuber water-use efficiency (WUEt), and biomass water-use efficiency (WUEb) across various Jerusalem artichoke genotypes with differing levels of drought tolerance. The experiment was conducted in pots using a 2 × 5 factorial combination in a randomized complete block design with four replications over two years. Factor A consisted of two water regimes: field capacity (FC) and mid-season drought. Factor B included five genotypes: JA 3, JA 125, JA 15, JA 89, and CN 52867. Mid-season drought significantly reduced tuber dry weight, biomass, WUEt, and WUEb, while increasing the harvest index. Significant differences were observed among genotypes for tuber dry weight, biomass, harvest index, WUEt, and WUEb under both water regimes. CN 52867 and JA 89 were characterized as drought-tolerant genotypes with high water-use efficiency and high yield potential. JA 3 was also noted for its lower yield reduction under stress. These three genotypes serve as valuable genetic resources for breeding programs aimed at developing progeny populations with enhanced yield potential and drought tolerance, particularly for mid-season drought-prone environments. Full article

Jerusalem artichoke (Helianthus tuberosus L.) is a plant with considerable potential for energy generation due to its rapid growth, high biomass yield, and resistance to environmental stresses. The aim of this study was to determine the influence of the nitrogen fertilization strategy on the yield and energy balance in the production technology of Jerusalem artichoke (JA) in a perennial cropping system. The article presents the results of a three-year experiment which was conducted in Poland to determine the effect of different N rates (0, 50, 75, and 100 kg ha⁻¹) supplied with mineral fertilizers and liquid digestate on the energy balance in the production of JA aerial biomass. The experiment had a randomized block design with three replications. The demand for energy in JA cultivation reached 16.2–26.3 (year 1) and 2.9–14.6 GJ ha⁻¹ (years 2 and 3). Energy inputs in the cultivation technology were reduced by 17–19% (year 1) and 35–47% (years 2 and 3) when mineral fertilizers were replaced with digestate. Jerusalem artichoke yields were lowest in the technology without fertilization (12.5 Mg ha⁻¹ DM). Dry matter yield increased significantly (by 43–55%) after the application of 75 kg N ha⁻¹, regardless of fertilizer type. The energy output of biomass peaked (230.1 GJ ha⁻¹) in response to a mineral fertilizer rate of 75 kg N ha⁻¹. In turn, the highest energy gain (218.5 GJ ha⁻¹) was noted after the application of digestate at a rate equivalent to 75 kg N ha^–1. The energy efficiency ratio was highest in the technology without fertilization (20.1) and after the application of digestate at a rate equivalent to 75 kg N ha⁻¹ (19.7). Regardless of the factors that limit agricultural production, the energy balance of JA biomass production was most favorable when JA was fertilized with digestate at a rate equivalent to 75 kg N ha⁻¹. The results of this study may pave the way for future research on novel agronomic strategies for sustainable bioenergy production, including nutrient recycling. Full article

Jerusalem artichoke (JA) (Helianthus tuberosus L., family Asteraceae) is an important feedstock for biofuel production due to its high biomass yield per unit area and the low costs associated with plantation establishment and cultivation technology. The chemical composition of the aerial biomass of JA grown in a perennial cycle and harvested once or twice during the growing season was determined, to assess the potential of JA for energy production. The experiment was conducted in 2018–2020 in north-eastern Poland. The study demonstrated that the crude ash (CA) content of the biomass was significantly (by 24.1%) higher when JA was harvested twice rather than once during the growing season, making it less suitable for energy purposes. However, double cutting induced an increase in the content of crude fiber (CFR), cellulose, and hemicellulose (by 87%, 41%, and 52%, respectively) in JA biomass compared with single cutting. In addition, twice-harvested JA biomass was also characterized by higher concentrations of neutral detergent fiber (NDF), acid detergent fiber (ADF), and acid detergent lignin (ADL) (by 40.7%, 38.9%, and 30.3%, respectively), and a lower (by 29.3%) concentration of water-soluble carbohydrates (WSC). These results indicate that the chemical composition of a JA biomass can be modified by selecting the appropriate harvest strategy, which is an important consideration for end users. Full article

This article presents the results of a three-year experiment (2018–2020) conducted at the Agricultural Experiment Station in Bałcyny (north-eastern Poland) with the aim of determining Jerusalem artichoke (JA) yields and the energy balance of biomass production in (i) a perennial cropping system (only aerial biomass was harvested each year) and (ii) an annual cropping system (both aerial biomass and tubers were harvested each year). When JA was grown as a perennial crop, the demand for energy reached 25.2 GJ ha⁻¹ in the year of plantation establishment and 12.3–13.4 GJ ha⁻¹ in the second and third year of production. The energy inputs associated with the annual cropping system were determined in the range of 31.4–37.1 GJ ha⁻¹. Biomass yields were twice as high in the annual than in the perennial cropping system (20.98 vs. 10.30 Mg DM ha⁻¹). Tuber yield accounted for 46% of the total yield. The energy output of JA biomass was 1.8 times higher in the annual than in the perennial cropping system (275.4 vs. 157.3 GJ ha⁻¹). The average energy gain in JA cultivation ranged from 140 (perennial crop) to 241 GJ ha⁻¹ (annual crop). The energy efficiency ratio of JA biomass production reached 7.7–13.3 in the perennial cropping system, and it was 20% lower in the annual cropping system. These results imply that when JA was grown as an annual crop, an increase in energy inputs associated with plantation establishment (tillage and planting) and the harvest and transport of tubers was not fully compensated by the energy output of tubers. Full article

Knowledge of the influence of different drought durations during the terminal growth stages on net photosynthetic rate (P_n), transpiration rate (Tr), transpiration efficiency (TE), and tuber fresh weight (economic yield) is relatively limited in Jerusalem artichoke. Our aim was to examine the effect of different drought durations during the terminal growth stages on P_n, Tr, TE, and economic yield in Jerusalem artichoke genotypes with different ranges of drought tolerance. A two-year field experiment was established in the northeast of Thailand. A split-plot design replicated four times was used. Three water treatments, optimal conditions (SD0), short-drought (SD1), and long-drought (SD2) durations during the terminal growth stages, were arranged as main plots. Six genotypes (HEL256, JA37, HEL253, JA4, JA60, and JA125) are subplots. Different durations of drought in SD1 and SD2 reduced P_n, Tr, and economic yield, while drought stress during the terminal growth stages increased TE in whole genotypes over SD0 treatments. Drought-tolerant genotypes JA125 and JA4 exhibited high P_n, medium TE, and low economic yield reduction under SD2. Utilization of these superior parents in breeding could lead to the generation of new progenies with high economic yield productivity and drought tolerance during the long drought duration during the terminal growth stage. In addition, high SPAD chlorophyll meter readings (SCMR) in SD1 and SD2 and low specific leaf area (SLA) in SD1 could be used as indirect selection traits to survey high P_n genotypes in Jerusalem artichoke. Consequently, these indirect selection traits may allow for speedy breeding programs in Jerusalem artichokes. Full article

JA (Jerusalem artichoke) cultivars react differently to mineral fertilization. Hence, the aim of this study was to assess the effect of mineral fertilization on the yield and yield structure of this species in terms of its aboveground biomass. The research was based on a field experiment carried out in 2016–2018 in Central and Eastern Poland using the randomized subblock method, in a split-split-plot-dependent system. The factors of the first-order experiment were the cultivars of Jerusalem artichoke (JA) (‘Albik’, ‘Rubik’ and ‘Violet de Renes’), while the second-order factors were differentiated mineral fertilizers (N₀P₀K₀ as a control object and P₄₃, K₁₂₄, N₁₀₀, P₄₃K₁₂₄, N₅₀P₄₃K₁₂₄m N₁₀₀P₄₃K₁₂₄, and N₁₅₀P₄₃K₁₂₄) converted to their elemental forms compared with the full dose of manure. The third-order factors were nitrogen forms, including ammonium nitrate and amide. The aboveground biomass yield and its structure were determined. The tested cultivars did not differ significantly in yield, but had different yield structures. The use of mineral fertilization brought measurable effects in the form of an increase in the yield of aboveground biomass. The ‘Albik’ cultivar responded best to mineral fertilization, with the highest yield of aboveground biomass being obtained after applying 150 kg N ha⁻¹ in the form of ammonium nitrate against the background of constant phosphorus–potassium fertilization. The ‘Rubik’ cultivar responded most favorably to the addition of 50 kg N ha⁻¹ in the amide form against the background of phosphorus–potassium fertilization, while the ‘Violet de Renes’ cultivar produced the highest yield of aboveground biomass after the application of 100 kg N ha⁻¹ in the amide form, including PK fertilization. It was found that it was advantageous to use a combination of nitrogen and phosphorus–potassium fertilization. Full article

Jerusalem artichoke (Helianthus tuberosus L.) is emerging as one of the energy plants considered for biofuel production. Alkali and alkali-involved pretreatment methods have been widely used for the bioconversion of cellulosic materials due to their high sugar yield and low inhibitor release. However, the recovery and treatment of wastewater (black liquor) have been poorly studied. Here, we present a novel two-stage pretreatment process design for recycling black liquor. Jerusalem artichoke stalk (JAS) was first treated with 2% (w/v) NaOH, after which lignin was recovered by H₂SO₄ at pH 2.0 from the black liquor. The recycled solutions were subsequently used to treat the NaOH-pretreated JAS for the second time to dissolve hemicellulose. CO-pretreated JAS, hydrolysates, and acid-insoluble lignin were obtained after the above-mentioned two-stage pretreatment. A reducing sugar yield of 809.98 mg/g Co-pretreated JAS was achieved after 48 h at 5% substrate concentration using a cellulase dosage of 25 FPU/g substrate. In addition, hydrolysates containing xylose and acid-insoluble lignin were obtained as byproducts. The pretreatment strategy described here using alkali and acid combined with wastewater recycling provides an alternative approach for cellulosic biorefinery. Full article

Jerusalem artichoke (Helianthus tuberosus L.), also called wild sunflower, belongs to the Asteraceae family and is cultivated widely across the temperate zone for its nutritious tuber, which is used as a root vegetable. In this study, the Jerusalem artichoke (JA) was used as a supplementary additive for producing a functional yogurt, with enhanced health benefits and improving the microbiological, rheological, and sensorial quality characteristics of the product. The effects of the three different concentrations (1%, 2%, and 3%, w/w) of JA on the physicochemical properties, bacterial counts, sensorial properties, and organic acid profiles of yogurts were determined after 1, 7, 14, and 21 days of storage at ±4 °C. The results obtained revealed that with the addition of different concentrations of JA the overall parameters were statistically significant (p < 0.01 and p < 0.05) except for apparent viscosity, Streptococcus thermophilus, yeast and mold count, pyruvic ratios, and scores of flavor. Similarly, some parameters (fat ratio, yeast and mold count, concentrations of propionic, acetic, pyruvic, orotic, and lactic acids, and scores of appearance, consistency, and odor) changed depending on the storage time, while some did not show any changes regarding storage time. There was a relationship found between the concentration of JA and organic acid ratio (except for pyruvic acid) concentration in the yogurt samples. In conclusion, the research revealed the effect of JA in yogurt production as a thickener, flavor enhancer, prebiotic agent, and source of organic acids and bioactive compounds. The results indicate that JA has a good potential for enhancing the nutritional and physicochemical properties of the studied yogurt. Full article

Research of new input raw materials for biogas plants is a very actual topic. There are only a very few studies dealing with the possibility of using silage prepared from the above-ground parts of the Jerusalem artichoke (Helianthus tuberosus L.) and maize (Zea mayse L.) for methane production. This study deals with the determination of methane production and methane content in biogas during the fermentation of maize silage with dissimilar additions of the biomass of the Jerusalem artichoke (JA). Except for the effect of the JA’s addition on the yield of methane, we also studied its potential influence on the inhibition of the process of anaerobic digestion and the bacterial and methanogenic archaeal composition of anaerobic digestate. There were five model silages prepared; two of them contained only maize or JAs, and the remaining three were mixtures of maize and JA silages (30%wt; 50%wt and 70%wt). The fermentation tests showed that the JA addition (from 30 to 70%wt) resulted in the production of biogas decreasing, on average, by 15%. Based on the performed metagenomic analysis, we cannot confirm an essential influence of JA biomass addition on the composition of the community of microorganisms during fermentation. Full article

The aim of the present study was to evaluate the effects of a Jerusalem artichoke-supplemented diet on the blood chemistry, growth performance, intestinal morphology, expression of antioxidant-related genes, and disease resistance against Aeromonas veronii challenge in juvenile red tilapia. A completely randomized design (CRD) was followed to feed red tilapias with three experimental diets: control, 5.0 g/kg JA-supplemented (JA5), or 10.0 g/kg JA-supplemented (JA10) diets in triplicates for 4 weeks. The results revealed that the growth performance, weight gain (WG), specific growth rate (SGR), and average daily gain (ADG) of fish fed diets JA5 and JA10 were significantly higher (p < 0.05) than those of fish fed the control diet. Fish fed the control diet had significantly higher T-bilirubin, D-bilirubin, and ALT in blood serum than fish fed JA5 and JA10, as well as higher BUN than fish fed JA5. The number of goblet cells in the proximal and distal parts of the intestine revealed that the number of acid, neutral, and double-staining mucous cells of fish fed diets JA5 and JA10 was significantly higher (p < 0.05) than in fish fed the control diet. The diets including the prebiotic (JA5 and JA10) were associated with a significant increase in the expression of gpx1 and gst antioxidant-related genes and disease resistance against A. veronii in juvenile red tilapia. Therefore, JA5 and JA10 can be employed as promising prebiotics for sustainable red tilapia farming. Full article

Jerusalem artichoke (Helianthus tuberosus L.) is considered to be one of the most promising multipurpose bioenergetic crops. The goal of this study was to carry out laboratory and field research regarding the tops and tubers of 16 Jerusalem artichoke (JA) cultivars grown on sod-podzolic sandy loam soils, taking into account varietal characteristics in order to point out advances in JA potential as alternative feedstock in herbivorous animal husbandry. The height of JA plants produced was from 147 to 280 cm. Having formed by the beginning of September, the size of the assimilating leaf apparatus surface was 0.41 to 2.31 m²/plant. In early September, the productivity of JA green mass amounted to 23.6 to 86.0 t/ha for late-maturing cultivars on average; correspondingly, this was 13.0 to 25.4 t/ha for early-maturing cultivars. At the end of October, the “late” cultivars produced 28.2 to 86.9 t/ha of green mass; on the contrary, the herbage of the “early” ones mostly withered and even dried up. The highest gross yield of tubers in early September was obtained from cultivars Diyeticheskiy (43.5 t/ha) and Nadezhda (40.8 t/ha). The average yield of early-maturing cultivars was 13.4 … 43.5 t/ha; as for “late” ones, it was reported to be 6.0 … 35.9 t/ha. In the third ten-day period of October, tubers of all cultivars gained weight significantly: the average yield of early-maturing cultivar tubers increased up to 33.1 …51.1 t/ha, whereas the average tuber yields of “late” cultivars were 14.4 … 43.9 t/ha. On average, the distribution of dry matter content in JA was 28.8 to 29.8% in aboveground biomass and in tubers, 23.8 to 24.0%. According to the chemical composition, cellulose (10.9 to 13.1%) and sugars (4.0 to 4.2%) could be noted to predominate in the green mass, but tubers were high in sugars (16.9 to 17.6%). The analyses on the trace elements (Ca, Fe, Mg, Mn, Si and Zn) showed that crop green mass contained more Mg (45 to 72 mg/100 g) and Mn (44 to 65 mg/100 g), but for JA tubers, late-maturing cultivars were rich in Si (27.2 to 79.0 mg/100 g) and early-maturing cultivars were mainly high in Zn (32.8 to 46.5 mg/100 g). The highest total coefficient of energy efficiency was displayed by the following cultivars: Novost VIRa (3.09); Tadzhikskiy (2.78); Spindle (2.68); Korenevskiy (2.43); Interes (2.10); and Skorospelka (1.98). In this respect, Jerusalem artichoke certainly has potential as a forage crop that can reach high yields with low external inputs. The data obtained may be useful for farmers who want to adapt and produce this useful and essential crop for the sustainability of feed production. Full article

The present research investigates the tuber proteome of the ‘medicinal’ plant Jerusalem artichoke (abbreviated as JA) (Helianthus tuberosus L.) using a high-throughput proteomics technique. Although JA has been historically known to the Native Americans, it was introduced to Europe in the late 19th century and later spread to Japan (referred to as ‘kiku-imo’) as a folk remedy for diabetes. Genboku Takahashi research group has been working on the cultivation and utilization of kiku-imo tuber as a traditional/alternative medicine in daily life and researched on the lowering of blood sugar level, HbA1c, etc., in human subjects (unpublished data). Understanding the protein components of the tuber may shed light on its healing properties, especially related to diabetes. Using three commercially processed JA tuber products (dried powder and dried chips) we performed total protein extraction on the powdered samples using a label-free quantitate proteomic approach (mass spectrometry) and catalogued for the first time a comprehensive protein list for the JA tuber. A total of 2967 protein groups were identified, statistically analyzed, and further categorized into different protein classes using bioinformatics techniques. We discussed the association of these proteins to health and disease regulatory metabolism. Data are available via ProteomeXchange with identifier PXD030744. Full article

One of the major challenges in PHA biotechnology is optimization of biotechnological processes of the entire synthesis, mainly by using new inexpensive carbon substrates. A promising substrate for PHA synthesis may be the sugars extracted from the Jerusalem artichoke. In the present study, hydrolysates of Jerusalem artichoke (JA) tubers and vegetative biomass were produced and used as carbon substrate for PHA synthesis. The hydrolysis procedure (the combination of aqueous extraction and acid hydrolysis, process temperature and duration) influenced the content of reducing substances (RS), monosaccharide contents, and the fructose/glucose ratio. All types of hydrolysates tested as substrates for cultivation of three strains—C. necator B-10646 and R. eutropha B 5786 and B 8562—were suitable for PHA synthesis, producing different biomass concentrations and polymer contents. The most productive process, conducted in 12-L fermenters, was achieved on hydrolysates of JA tubers (X = 66.9 g/L, 82% PHA) and vegetative biomass (55.1 g/L and 62% PHA) produced by aqueous extraction of sugars at 80 °C followed by acid hydrolysis at 60 °C, using the most productive strain, C. necator B-10646. The effects of JA hydrolysates on physicochemical properties of PHAs were studied for the first time. P(3HB) specimens synthesized from the JA hydrolysates, regardless of the source (tubers or vegetative biomass), hydrolysis conditions, and PHA producing strain employed, exhibited the 100–120 °C difference between the T_melt and T_degr, prevailing of the crystalline phase over the amorphous one (C_x between 69 and 75%), and variations in weight average molecular weight (409–480) kDa. Supplementation of the culture medium of C. necator B-10646 grown on JA hydrolysates with potassium valerate and ε-caprolactone resulted in the synthesis of P(3HB-co-3HV) and P(3HB-co-4HB) copolymers that had decreased degrees of crystallinity and molecular weights, which influenced the porosity and surface roughness of polymer films prepared from them. The study shows that JA hydrolysates used as carbon source enabled productive synthesis of PHAs, comparable to synthesis from pure sugars. The next step is to scale up PHA synthesis from JA hydrolysates and conduct the feasibility study. The present study contributes to the solution of the critical problem of PHA biotechnology—finding widely available and inexpensive substrates. Full article

In many countries, Jerusalem artichoke (JA) is a source of biomass for renewable energy production and alternative biofuel feedstock, and it is used for feed and food production. The species also has medicinal properties, and it is used in soil reclamation. The aim of this study was to evaluate the effect of N fertilization on the yield and macronutrient concentrations in JA tubers. The effect of N fertilization (control plot, unfertilized, 80 and 120 kg ha⁻¹) on aerial biomass yield, tuber yield, and the mineral composition of tubers in three JA cultivars (“cv.”) (Rubik, Albik, and Gute Gelbe) was investigated in a field experiment. Tuber yield (40.99 Mg ha⁻¹) and aerial biomass yield (62.76 Mg ha⁻¹) were highest in cv. Gute Gelbe fertilized with 120 kg N ha⁻¹ in the warm and moderately wet growing season of 2018. Agronomic N-use efficiency (AE) was highest in cv. Gute Gelbe. In the treatment supplied with 80 kg N ha⁻¹, the fresh matter yield (FMY) of tubers was determined at 66.4 kg kg⁻¹ N, whereas in the treatment fertilized with 120 kg N ha⁻¹, the FMY of tubers reached 101.8 kg kg⁻¹ N. The evaluated JA cultivars differed in their responses to an increase in the N fertilizer (marginal efficiency—ME) rate from 80 to 120 kg ha⁻¹. The strongest response was observed in cv. Gute Gelbe, where the tuber yield increased by 172.6 kg kg⁻¹ N. The tubers of cv. Gute Gelbe were characterized by significantly higher concentrations of N, K, Mg, and S compared with the other cultivars. The concentrations of macronutrients in the tubers (without Mg) were higher in spring. Nitrogen fertilization did not cause differences in the concentrations of P, K, Ca, Mg, and S, but it increased the N concentration in tubers. Full article