Search Results (365)

Aronia melanocarpa (black chokeberry) is a medicinally valuable small fruit species, yet its commercial propagation remains limited by low rooting and genotype-specific responses. This study developed an efficient, callus-free micropropagation and rooting protocol using a Shrub Plant Medium (SPM) supplemented with 5 mg/L BAP in large 660 mL jars, which yielded up to 27 shoots per explant. Optimal rooting (100%) was achieved with 0.5 mg/L NAA + 0.25 mg/L IBA in half-strength SPM. In the second phase, supervised machine learning models, including Random Forest (RF), XGBoost, Gaussian Process (GP), and Multilayer Perceptron (MLP), were employed to predict morphogenic traits based on culture conditions. XGBoost and RF outperformed other models, achieving R² values exceeding 0.95 for key variables such as shoot number and root length. These results demonstrate that data-driven modeling can enhance protocol precision and reduce experimental workload in plant tissue culture. The study also highlights the potential for combining physiological understanding with artificial intelligence to streamline future in vitro applications in woody species. Full article

The accurate dosing of polyaluminum chloride (PAC) is essential for achieving effective coagulation in drinking water treatment, yet conventional methods such as jar tests are limited in their responsiveness and operational efficiency. This study proposes a hybrid modeling framework that integrates artificial neural networks (ANN) with genetic algorithms (GA) to optimize PAC dosage under variable raw water conditions. Operational data from 400 jar test experiments, collected between 2022 and 2024 at the Yanahurco water treatment plant (Ecuador), were used to train an ANN model capable of predicting six post-treatment water quality indicators, including turbidity, color, and pH. The ANN achieved excellent predictive accuracy (R² > 0.95 for turbidity and color), supporting its use as a surrogate model within a GA-based optimization scheme. The genetic algorithm evaluated dosage strategies by minimizing treatment costs while enforcing compliance with national water quality standards. The results revealed a bimodal dosing pattern, favoring low PAC dosages (~4 ppm) during routine conditions and higher dosages (~12 ppm) when influent quality declined. Optimization yielded a 49% reduction in median chemical costs and improved color compliance from 52% to 63%, while maintaining pH compliance above 97%. Turbidity remained a challenge under some conditions, indicating the potential benefit of complementary coagulants. The proposed ANN–GA approach offers a scalable and adaptive solution for enhancing chemical dosing efficiency in water treatment operations. Full article

This study explores the application of Eye-Tracking technology for the ergonomic evaluation of assistive device usability. Sixty-four participants evaluated six jar-opening devices in a two-phase study. First, the participants’ gaze was recorded while they viewed six rendered pictures of assistive devices, each shown in two different versions: with and without rubber in the grip area. Second, the participants physically interacted with the devices in a hands-on usability task. In both phases, participants rated the devices according to six usability affordances: robustness, comfort, easiness to grip, lid slippery, effort level, and easiness to use. Eye-Tracking metrics (fixation duration, number of fixations, and visit duration) correlated with the on-screen ratings, which aligned with ratings after using the physical devices. High ratings in comfort and effort level correlated with more visual attention to the grip area, where the rubber acted as key signifier. Heatmaps revealed the grip area as important for comfort and easiness to use and the lid area for robustness and slipperiness. These findings demonstrate the potential of Eye Tracking in usability studies, providing valuable insights for the ergonomic evaluation of assistive devices. Moreover, they highlight the suitability of Eye Tracking for early-stage design evaluation, offering objective metrics to guide design decisions and improve user experience. Full article

Since its introduction into the USA, the spotted lanternfly (SLF), Lycorma delicatula, (White) (Hemiptera: Fulgoridae) has spread across the landscape relatively unchecked. With a wide host range, it is considered a serious pest of native forest species, as well as agricultural crops. Circle traps placed on Ailanthus altissima (Miller) Swingle (Sapindales: Simaroubaceae) are passive traps collecting SLF as they walk up and down the tree trunk. These traps are successful at detecting new populations of SLF, but this can be challenging to implement at a large scale due to costs and host availability. To improve and facilitate SLF trapping practices, we investigated three key trapping components: improved collection containers, placement on alternative hosts, and lure (methyl salicylate) impact. In initial trials comparing collection jars to removable plastic bags, the adult SLF catch was four times higher using the bag design. In a multi-state survey at varying population densities, the bag traps were comparable to the jar traps but were significantly more effective than BugBarrier^® tree bands, especially during the adult stage. Catch and detection in circle traps placed on alternative hosts, Acer spp. L. (Sapindales: Sapindalaceae) and Juglans nigra L. (Fagales: Juglandaceae), were comparable to those placed on the preferred host A. altissima, especially in the earlier life stages. Additionally, detection rates of methyl salicylate-baited traps on all three hosts were comparable to those on non-baited traps. These results suggest that circle traps fitted with bags provide higher trap catch and an improvement in sample quality. In addition, circle traps were equally effective when placed on maple and black walnut, while methyl salicylate lures do not enhance trap catch or detection. Full article

Natural organic matter (NOM) in surface waters is a major challenge for drinking water treatment due to its role in the formation of disinfection byproducts (DBPs) during chlorination. This study evaluated the performance of chitosan, a biodegradable coagulant, dissolved in acetic, lactic, and L-ascorbic acids for NOM removal under three turbidity levels (403, 1220, and 5038 NTU). Jar tests were conducted using raw water from the Río Grande de Añasco (Puerto Rico), and TOC, DOC, and UV₂₅₄ were measured at multiple time points. TOC removal ranged from 39.8% to 74.3%, with the highest performance observed in high-turbidity water treated with chitosan–L-ascorbic acid. DOC and UV₂₅₄ reductions followed similar trends, with maximum removals of 76.4% and 76.2%, respectively. Estimated THM formation potential (THMFP) was reduced by up to 81.6%. Across all acids, flocculation efficiencies exceeded 95%. Compared to conventional aluminum-based coagulants, chitosan demonstrated comparable performance, while offering environmental benefits. These results confirm the potential of chitosan–acid systems for effective organic matter removal and DBP control, supporting their application as sustainable alternatives in drinking water treatment. Full article

Polyembryonic maize, capable of producing multiple seedlings from a single kernel, holds great potential value in agricultural and industrial applications, but the seedling quality needs to be improved. In this study, seedlings of two waxy maize (Zea mays L. sinensis Kulesh) inbred lines, D35 (a polyembryonic line with twin shoots) and N6110 (single-shoot), exhibited similar relative growth rates during 1 to 5 days post-germination. UPLC-MS/MS profiling of 3- to 5-day-old seedling roots and shoots revealed that H2JA, MeSAG, and IAA-Val-Me were the common differentially accumulated metabolites (DAMs) of the 3-day-old vs. 5-day-old seedlings of D35 and N6110 in the same tissues, and MeSAG, tZ9G, cZROG, and DHZROG were identified in D35 vs. N6110 across the same tissues and the same periods. RNA-seq analyses showed various processes involved in seedling development, including DNA replication initiation, rhythmic processes, the cell cycle, secondary metabolic processes, and hormone biosynthetic regulation. The differentially expressed genes (DEGs) between D35 and N6110 were significantly enriched in organic hydroxy compound biosynthetic, alcohol biosynthetic, organic hydroxy compound metabolic, abscisic acid biosynthetic, and apocarotenoid biosynthetic processes. The KEGG-enriched pathways of DAMs and DEGs identified that AUX1, AHP, A-ARR, JAR1, SIMKK, ERF1, and GID2 might be conserved genes regulating seedling growth. The integrated analyses revealed that 98 TFs were potentially associated with multiple hormones, and 24 of them were identified to be core genes, including 11 AP2/ERFs, 4 Dofs, 2 bZIPs, 2 MADS-box genes, 2 MYBs, 1 GATA, 1 LOB, and 1 RWP-RK member. This study promotes a valuable understanding of the complex hormone interactions governing twin-shoot seedling growth and offers potential targets for improving crop establishment via seedling quality. Full article

Viscous properties play a major role in the time-dependent deformation behavior of polymers and have long been characterized using spring-dashpot models. However, such models face a bottleneck of having multiple sets of model parameter values that can all be used to simulate the same deformation behavior. As a result, these model parameters have not been widely used to quantify the viscous properties. In this study, a newly developed multi-relaxation-recovery test was used to obtain the variation in stress response to deformation of polyethylene (PE) and its pipes during relaxation, revealing the complexity of PE’s nonlinear viscous stress response to deformation. Using a three-branch spring-dashpot model with two Eyring’s dashpots, this study shows the possibility of determining the model parameter values using four different analysis methods, namely, the mode method, peak-point method, highest-frequency method, and best-five-fits method. Model parameter values from these methods are compared and discussed in this paper, to reach the conclusion that the best-five-fits method provides the most reliable and relatively unique set of model parameter values for characterizing the mechanical performance of PE and its pipes. The best-five-fits method is expected to enable the use of the model parameters to quantify PE’s viscous properties so that PE’s load-carrying performance can be properly characterized, even for long-term applications. Full article

Background/Objectives: Proximal interphalangeal joint (PIJ) arthrodesis is a common surgical intervention for patients with PIJ osteoarthritis or trauma-related joint destruction. The objective of this study was to evaluate the biomechanical stability of various arthrodesis techniques under forces comparable to activities of daily living (ADL) to assess their suitability for early active movement protocols. Methods: In this in vitro study, composite cylinders simulating PIJ arthrodesis were subjected to standardized fusion angles of 40° using different fixation techniques, including crossed K-wires, compression screws, cerclage wires, tension band wiring, anatomical fixation plates, and locking grid plates. Forces representing ADLs such as typing, holding a pencil, carrying weight, and opening a jar were applied using a universal testing machine in a four-point bending setup. Micromotion and gap clearance were calculated and analyzed. Results: Techniques involving compression, such as compression screws, tension bands, and cerclage wires, exhibited lower micromotion and gap clearance under forces up to 17 N, suggesting potential suitability for early active movement protocols. In contrast, fixation plates demonstrated structural failure or excessive clearance during early active motion ADLs. K-wires showed intermediate results with moderate gap clearance and micromotion. Conclusions: Compression-based fixation techniques for PIJ arthrodesis may permit early active movement without external stabilization, while fixation plates are prone to failure under ADL forces. Further dynamic biomechanical testing and clinical studies are recommended to confirm these findings. Full article

Dikili Tash is a Neolithic settlement that lies next to the ruins of the ancient city of Philippi on the north-eastern part of Greece. A recent archaeological excavation has unearthed charred grapevine pips and pressings together with two-handed clay cups, jugs, and jars that date to 4300 BC. The majority of the pips were found to be Vitis vinifera ssp. sylvestris. Natural populations of this species have been localized in the valley surrounding Dikili Tash and also on Mt Pangaion and Mt Lekani, which flank the valley. Fifty-one samples from these modern populations have been analyzed using microsatellites on twenty microsatellite loci, and a dendrogram has been constructed showing the genetic closeness of the samples analyzed. Cuttings from all the vines analyzed are currently rooted and grown in the Hellenic Agricultural Organization—DIMITRA (ELGO-DIMITRA) greenhouse facilities in Lykovryssi (Athens) with the aim to, eventually, be transplanted in the grapevine, thus establishing the first V. sylvestris ex situ conservation site in Greece. Full article

The production of healthy propagating material of the potato (Solanum tuberosum L.) is based on in vitro micropropagation. In vitro conditions, however, can cause stress leading to reduced quality, growth and development of in vitro plantlets. The effects of aeration and chemical additives on the in vitro growth and development and quality of potato plantlets were investigated. Four different jar closure types were tested, i.e., an intact metal cap (control), two layers of semi-permeable plastic foil, a metal cap with a single hole, or a metal cap with three holes. Under tightly sealed conditions (intact metal cap) the effects of silver nitrate (2.0 mg L⁻¹) and 1-naphtylacetic acid (0.1 mg L⁻¹) alone or in combination with each other, meta-topoline (0.1 mg L⁻¹), ascorbic acid (10.0 mg L⁻¹), salicylic acid (0.1 mg L⁻¹), jasmonic acid (0.1 mg L⁻¹) and glutamic acid (0.3 mg L⁻¹) were studied. Morpho-physiological parameters were measured at the end of the subculture. Leaf development was a good indicator of the presumed ethylene effect. The development and quality of the plantlets were best in cultures sealed with three-holed caps. Of the chemicals applied, only the presence of silver nitrate resulted in high-quality plantlets. The combined application of silver nitrate and 1-naphthaleneacetic acid promoted root growth and development. Full article

High turbidity in raw water poses a major challenge to drinking water quality and requires effective, sustainable treatment solutions. This work investigates the reduction in turbidity in raw water and the enhancement of overall drinking water quality through the coagulation–flocculation process. The performance of Pine cone extract as a bio-coagulant was evaluated using four different solvent-based extractions (PC-H₂O, PC-HCl, PC-NaCl, and PC-NaOH). The effects of key operational parameters were analyzed, and jar tests were carried out to enhance the coagulation–flocculation process by identifying the optimal conditions. Experimental design was further refined using RSM based on a BBD, incorporating three factors: initial pH, coagulant dosage, and settling time, with turbidity removal efficiency as the response variable. Statistical analysis confirmed that initial pH, coagulant dosage, and settling time significantly influenced turbidity reduction at a confidence level of p-value < 0.05 for all four solvents. Among the extracts tested, PC-HCl demonstrated the highest turbidity removal efficiency. The optimal conditions achieving 78.57% turbidity reduction were a pH of 8.5, a coagulant dosage of 100 mL/L, and a settling time of 120 min. These findings highlight the significant potential of Pine cone extract as an effective, sustainable, and eco-friendly organic coagulant for raw water treatment. Full article

The work presented here describes an approach that separates the viscous stress from the quasi-static counterpart for polycarbonate (PC) and its short glass fiber composite (GF-PC), with the aim to characterize the influence of short glass fiber on the viscous behavior of PC as the matrix of GF-PC. A multi-relaxation (MR) test was used for the mechanical testing and a three-branch spring–dashpot model for the data analysis, using a genetic algorithm to establish 100 sets of fitting parameter values that enabled the three-branch model to regenerate the measured stress decay during relaxation. Using the spring modulus $\left(K_{v,s}\right)$ of the short-term branch in the three-branch model, two groups for these fitting parameter values were established as a function of specimen displacement (named stroke) of GF-PC, one of which shows a trend that is similar to the trend of the corresponding fitting parameters for the pure PC, and thus is believed to reflect the influence of glass fiber on the PC matrix of GF-PC. The study concludes that the short glass fiber increases the short-term viscous stress, but its role on the long-term viscous stress is marginal. Full article

A copolymer of 3-hydroxybutyrate (3HB) and 3-hydoxyhexanoate (3HHx), PHBHHx, is a practical biodegradable plastic, and at present, the copolymer is produced at commercial scale via heterotrophic cultivation of an engineered strain of a facultative hydrogen-oxidizing bacterium, Cupriavidus necator, using vegetable oil as the carbon source. In our previous report, we investigated PHBHHx production from CO₂ via pH-stat jar cultivation of the newly created recombinants of C. necator under autotropic conditions, feeding the inorganic substrate gas mixture (H₂/O₂/CO₂ = 80:10:10 v/v%) into a recycled-gas closed-circuit (RGCC) culture system. The dry cell weight (DCW) and PHBHHx concentration with the best strain MF01/pBPP-ccr_MeJ_Ac-emd increased to 59.62 ± 3.18 g·L⁻¹ and 49.31 ± 3.14 g·L⁻¹, respectively, after 216 h. In this study, we investigated the high-concentration production of PHBHHx with a shorter cultivation time by using a jar fermenter equipped with a basket-shaped agitator to enhance oxygen transfer in the culture medium and by continuously supplying the gases with higher O₂ concentrations to maintain the gas composition within the reservoir at a constant ratio. The concentrations of ammonium and phosphate in the culture medium were maintained at low levels. As a result, the DCW and PHBHHx concentrations increased to 109.5 ± 0.30 g·L⁻¹ and 85.2 ± 0.62 g·L⁻¹ after 148 h, respectively. The 3HHx composition was 10.1 ± 0.693 mol%, which is suitable for practical applications. Full article

It is known that nutrient deprivation following detachment can cause non-chilling peel pitting (NCPP) in citrus fruits when stored under a non-stressful environment and that this damage is reduced by pretreating the fruit with ethylene (ETH) (4 d, 10 µL L⁻¹). The present work investigates the effect of this pretreatment on jasmonate (JA) accumulation and transcriptional regulation in mature Navelate oranges (Citrus sinensis L. Osbeck) stored under non-stressful conditions. ETH increased the expression of abundant genes participating in the synthesis of cis-(+)-12-oxo-phytodienoic acid (OPDA), jasmonic acid (JA), and methyl jasmonate (MeJA). ETH also upregulated genes involved in jasmonoyl–isoleucine (JAIle) synthesis (CsJAR1) and decrease (CsCYP94B3 and CYP94C1), and CsSTA2, related to JA sulfation. The levels of these JA metabolites increased during fruit holding in ETH and after shifting them to air, with MeJA accumulation being especially remarkable. Overall, the beneficial effect of ETH on reducing NCPP appears to be related not only to this redirection of OPDA and JA metabolism towards the formation of JA derivatives but also to the regulation of JA signalling. Indeed, the repression of the receptor CsCOI1 and upregulation of various CsJAZs repressors caused by nutrient deprivation, together with the ETH-mediated induction of CsCOI1, CsTOPLESS, and abundant CsJAZs during long-term storage, suggests the occurrence of an ETH-enhanced negative transcriptional regulatory feedback loop in JA metabolism and signalling, by which the susceptibility of detached Navelate oranges to NCPP might be reduced. Full article

Fruits, such as apricots, cherries, raspberries, and strawberries, are very often processed into jams to extend their shelf life. Jams are highly appreciated by consumers, and their storage conditions are important for preserving their quality. This study investigates the impact of storage on the microbiological quality and physicochemical parameters of five commercial fruit (apricot, sour cherry, white cherry, raspberry, and strawberry) jams. The pH, titratable acidity, moisture, sugar content, viscosity, and color were evaluated immediately after opening the jam containers and during storage at 2–4 °C and 20 °C. The total number of mesophilic aerobic germs (TMAG) and the total number of yeasts and molds (TYM) were also determined. The samples were analyzed during storage at 14, 42, and 56 days after opening the jam jars. The pH of the fruit jam samples varied from 2.91 (strawberry jam) to 3.47 (sour cherry jam) and decreased during storage, while the titratable acidity (TA) ranged from 0.46 (cherry and raspberry jams) to 0.52% malic acid (apricot jam) and increased during storage, regardless of the storage temperature. The moisture content was between 15.78% (apricot jam) and 21.82% (raspberry jam), and decreased in all the jam samples during storage. The sugar content (30.40–44.59 g per 100 g of jam) was typical for low-sweetened jams and increased during storage. Also, the viscosity of the fruit jam samples increased during storage (more in the samples stored at room temperature). Under the storage conditions, all the jam samples lost their specific color. Immediately after opening the jam jars, no yeasts or molds were found in the apricot and sour cherry jam samples. The highest number of yeasts and molds was detected in the white cherry jam samples (4.25 log₁₀ CFU/g). The TYM increased during storage, as did the TMAG. The time–temperature interaction factor influenced the physicochemical and microbiological properties of the jam samples. Full article