Search Results (91)

This study aimed to evaluate the forage yield, quality, and weed suppression potential of narbon vetch (Vicia narbonensis L.) and Italian ryegrass (Lolium multiflorum L.) grown as sole crops and in mixtures under organic farming conditions in Bilecik, Turkey, during the 2020–2021 growing season. The experiment included 15 treatments comprising monocultures and mixed sowing at different ratios. Measurements included morphological traits, forage yield components (green herbage, hay, and crude protein), fiber content, botanical composition, and weed biomass. The results reveal significant differences among treatments in terms of growth parameters and forage performance. Monocultures of IFVN 567 and Bartigra showed the highest green and hay yields, while mixtures such as IFVN 567 + Trinova and IFVN 567 + Bartigra outperformed in terms of land equivalent ratio (LER) and protein yield, demonstrating a clear advantage in land use efficiency. Furthermore, these mixtures showed superior weed suppression compared to monocultures. Overall, the findings suggest that carefully selected vetch–ryegrass combinations can enhance forage productivity, nutritional quality, and weed management under organic systems. Full article

Plant roots influence various soil physical properties by altering the soil structure and pore configuration; however, a detailed understanding of these effects remains limited. In this study, we applied a relatively simple approach for segmenting plant roots and soil constituents using X-ray computed tomography (CT) images to evaluate root-induced changes in soil structure. The method combines manual initialization with a layer-wise automated region-growing approach, enabling the extraction of the root systems of soybean, Italian ryegrass, and Guinea grass. The method utilizes freely available software with a simple interface and does not require advanced image analysis skills, making it accessible to a wide range of researchers. The soil particles, pore water, and pore air were segmented using a Kriging-based thresholding technique. The segmented four-phase images allowed for the quantification of the volume fractions of soil constituents, pore size distributions, and coordination numbers. Furthermore, by separating the rhizosphere and bulk soil, we found that the root presence significantly reduced solid fractions and increased water content, particularly in the upper soil layers. Macropores and fine pores were observed near the roots, highlighting the complex structural impacts of root growth. While further validation is needed to assess the method’s applicability across different soil types and imaging conditions, it provides a practical basis for visualizing and quantifying root–soil interactions, and could contribute to advancing our understanding of how plant roots influence key soil hydraulic and thermal properties. Full article

In integrated crop–livestock systems (ICLSs), grazing intensity and crop rotation influence residue dynamics, making it essential to assess shoot and root contributions to soil carbon (C) and nitrogen (N) inputs. This study aimed to assess the shoot and root biomass of Italian ryegrass, soybean, and maize; the distribution of roots within the soil profile; and the contributions of shoot and root biomass to soil C and N under varying winter grazing intensities and summer crop rotations. The experiment was conducted within a long-term (12-year) field protocol, arranged in a randomized complete block design with split plots and four replicates. Grazing intensity was defined as the following: (i) moderate grazing—forage allowance equivalent to 2.5 times the potential dry matter intake of sheep, and (ii) low grazing—forage allowance equivalent to 5.0 times the intake potential. Grazing intensities (moderate and low) were allocated to the main plots, while cropping systems—monoculture (soybean/soybean) and crop rotation (soybean/maize)—were assigned to the subplots. Soil depth layers (0–10, 10–20, 20–30, and 30–40 cm) were treated as sub-subplots. Root samples of Italian ryegrass, soybean, and maize were collected using the soil monolith method. Low grazing intensity (8.6 Mg ha⁻¹) promoted greater aboveground biomass production of Italian ryegrass compared to moderate intensity (6.6 Mg ha⁻¹). Maize exhibited a higher capacity for both root and shoot biomass accumulation, with average increases of 85% and 120%, respectively, compared to soybean. Root biomass was primarily concentrated in the surface soil layer, with over 70% located within the top 10 cm. Italian ryegrass showed a more uniform root distribution throughout the soil profile compared to soybean and maize. Carbon inputs were higher under crop rotation (17.2 Mg ha⁻¹) than under monoculture (15.0 Mg ha⁻¹), whereas nitrogen inputs were greater in soybean monoculture (0.23 Mg ha⁻¹) than in crop rotation (0.16 Mg ha⁻¹). Low grazing intensity in winter and summer crop rotation with high-residue and quality species enhance the balance between productivity and soil C and N inputs, promoting the sustainability of ICLSs. Full article

A field experiment was conducted in two seasons (2019–2020 and 2020–2021) at three locations in Slovenia (Rogoza, Fala, and Brežice) to evaluate the yield and silage quality of winter cover crops (WCCs). The experiment included Italian ryegrass (IR) in pure stands, fertilized with nitrogen in spring, and mixtures of crimson clover (CRC), red clover (RC), and IR+CRC+RC without nitrogen fertilization in spring. The highest dry matter yield (DMY) was observed in IR+CRC+RC (4.98 t ha⁻¹). For fresh feed, the CRC+RC treatment had significantly higher (p < 0.05) crude protein (208 g kg⁻¹ DM), nitrate nitrogen (116.7 mg kg⁻¹ DM), and buffering capacity (1290 mmol kg⁻¹ DM) but significantly lower (p < 0.05) dry matter (128 g kg⁻¹) and water-soluble carbohydrates (121 g kg⁻¹ DM). For silage, the CRC+RC treatment had significantly lower (p < 0.05) dry matter (476 g kg⁻¹ silage), metabolic energy (9.65 MJ kg⁻¹ DM), net energy of lactation (5.77 MJ kg⁻¹ DM), and neutral detergent fiber (375 g kg⁻¹ DM) but higher ammonia nitrogen (66.5 g kg⁻¹ of total nitrogen), crude protein (158 g kg⁻¹ DM), and acid detergent fiber (279 g kg⁻¹ DM). No significant differences (p > 0.05) were found among treatments for acetic, lactic, and butyric acid, crude fat, pH, and soil mineral nitrogen (N_min). The results of the study show that the same or higher DMY and a comparable quality of highly wilted silage can be produced with mixed Italian ryegrass and clovers compared with those of Italian ryegrass in pure stands. The experiment aimed to determine whether clover-based mixtures can achieve comparable silage quality and dry matter yield without spring N fertilizers compared with those of pure stands of Italian ryegrass fertilized in spring. Full article

Italian ryegrass (Lolium multiflorum, IRG) and perennial ryegrass (Lolium perenne L., PRG) are widely cultivated as forage grasses in Korea using heterogeneous and polycross techniques, which promote genetic diversity within varieties. However, their genetic diversity patterns in Korea remain underexplored. This study evaluated the genetic diversity of IRG (eight varieties, including one exotic) and PRG (two exotic varieties) using 66 simple sequence repeat (SSR) markers. Across 87 samples (nine IRG and two PRG varieties), 655 alleles were identified, averaging 9.9 per locus. Key genetic parameters included heterozygosity (0.399), observed heterozygosity (0.675), fixation index (0.4344), and polymorphic informative content (0.6428). The lowest within-variety genetic distance was observed in ‘Hwasan 104ho’ (0.469), while ‘IR901’ had the highest (0.571). Between varieties, the closest genetic distance was between ‘Greencall’ and ‘Greencall 2ho’ (0.542), and the furthest was between ‘Kowinmaster’ and ‘Aspire’ (0.692). Molecular variance analysis showed 90% variation within varieties and 10% among varieties. Five clusters (I–V) were identified, with cluster I primarily including diploid IRG varieties and the tetraploid ‘Hwasan 104ho.’ Structural analysis differentiated diploid from tetraploid varieties (K = 2) and further separated tetraploid IRG and PRG (K = 3). Principal component analysis confirmed these groupings, with ‘Greencall’ and ‘Greencall 2ho’ exhibiting the closest genetic distance (0.227) and ‘Greencall’ and ‘Aspire’ the furthest (0.384). These findings provide a foundational resource for marker-assisted breeding to improve agronomic traits and enhance the efficiency of ryegrass breeding programs. Full article

Italian ryegrass is a high-quality forage grass, and a full understanding of the changes in its microbiome and metabolome during aerobic exposure can prolong its aerobic stability and improve its utilization value. Italian ryegrass silage was prepared with deionized water (CK), Lactobacillus rhamnosus BDy3-10 (LR), Lactobacillus buchneri TSy1-3 (LB), and a mixture of these two lactic acid bacteria (M). The silage was maintained at ambient temperature for 60 days followed by aerobic exposure. The results show that the Italian ryegrass silage in the LB and M groups exhibited aerobic stability for up to 19 days. A total of 1881 chemicals were identified in Italian ryegrass silage. These metabolites are associated with bacterial communities, especially Lactobacillus. The addition of lactic acid bacteria resulted in a common differential metabolic pathway compared to CK: “phenylpropanoid biosynthesis”. “Flavone and flavonol biosynthesis” was the significant differential metabolic pathway between LB and LR. Inoculation with LB significantly increased the concentrations of lactic acid, acetic acid, vitexin, and luteolin. In conclusion, lactic acid bacteria (LAB) additives affect the microbial community and metabolites of silage. The application of LB inoculants is a feasible way to obtain well-fermented Italian ryegrass silage and improve aerobic stability, even at higher moisture content levels. Full article

Many endophytic fungi are approved as plant growth stimulants, and several commercial biostimulants have already been introduced in agricultural practice. However, there are still many species of fungi whose plant growth-promoting properties have been understudied or not studied at all. We examined the growth-promoting effect in spring barley (Hordeum vulgare) and Italian ryegrass (Lolium multiflorum) induced by three endophytic fungi previously obtained from the roots of Festuca/Lolium grasses. Surface-sterilized seeds were inoculated with a spore suspension of Cadophora fastigiata (isolate BSG003), Paraphoma fimeti (BSG010), Plectosphaerella cucumerina (BSG006), and their spore mixture. Before harvesting, the inoculated plants were grown in a greenhouse, with the barley being in multi-cavity trays for 30 days and ryegrass being placed in an original cylindric element system for 63 days. All three newly tested fungi had a positive effect on the growth of the barley and ryegrass plants, with the most pronounced impact observed in their root size. The fungal inoculations increased the dry shoot biomass between 11% and 26% in Italian ryegrass, but no such impact was observed in barley. The highest root increment was observed in barley. Herein, P. cucumerina and C. fastigiata inoculations were superior to other treatments, showing an increase in root dry weight of 50% compared to 20%, respectively. All fungal inoculations significantly promoted root growth in Italian ryegrass, resulting in a 20–30% increase in dry weight compared to non-inoculated plants. Moreover, a strong stimulatory effect of the fungi-emitted VOCs on the root development was observed in plate-in-plate arrays. In the presence of C. fastigiata and P. cucumerina cultures, the number of roots and root hairs in barley seedlings doubled compared to control plants. Thus, in our study, we demonstrated the potential of the grass root-derived endophytes C. fastigiata, P. fimeti, and P. cucumerina as growth promoters for spring barley and Italian ryegrass. These studies can be extended to other major crops and grasses by evaluating different fungal isolates. Full article

Rice is a staple food in Asia, and its impact on the environment is considerable, such as chemical input concerns. Organic rice farming represents an alternative approach to reducing environmental concerns throughout rice production. However, the precise nutrient management to optimize organic rice production while recovering soil residual nitrogen (N) for the subsequent crops remains unclear. This study aims to: (1) assess nutrient recovery in soil cultivated with cover crops, including Italian ryegrass and hairy vetch, and (2) investigate the optimization of nutrient management in organic rice farming using cover crops. An experiment was conducted in a paddy field adopting cover crop plots and fallow (FA) plots in four replicates each from 2021 to 2023. In addition, incubation studies were conducted in 2021 and 2022. The incubation study included various treatments: (1) soil from cover crop or FA plots, (2) with or without cover crop residues, (3) with or without weed input (2021). In 2022, fertilizer input replaced weed input. The field study indicated cover crop biomass was larger than that of weeds. Furthermore, it can determine cover crops have more recyclable plant N compared to weeds when incorporated into the soil. In contrast, there was no noticeable difference in soil inorganic N and soil total organic carbon (C) contents between cover crop and FA plots at the 0–90 cm depth. In the incubation study, we found the soil of cover crop plots and cover crop input show less inorganic N than the soil of FA plots and cover crop input during the incubation period. However, the soil of the cover crop plots and cover crop input showed a high inorganic N content after setting the flooded condition. It indicates the soil of cover crop plots, and cover crop input provides N to the soil for a longer period. Overall, our results show that winter cover crop application in paddy fields contributes to N recovery and helps maintain soil fertility. Specifically, the occasional cultivation of a combination of Italian ryegrass and hairy vetch as winter cover crops can contribute to reducing the reliance on chemical fertilizers. This practice also promotes sustainable rice farming in paddy fields. Full article

The challenge of today’s agriculture is to maintain stable production and at the same time improve soil conditions. Appropriate crop management can contribute to the increase of yields, nutritional status of plants, and below-ground biomass which consequently increases soil organic carbon (SOC). One promising approach to increase yield and reduce the use of synthetic fertilizers involves using plant growth-promoting microorganisms. The present study explores the possibility of applying microbial bioagents as a sustainable alternative to synthetic fertilizers in Italian ryegrass cultivation. Four microbial formulations, consisting of nitrogen-fixing bacteria, phosphate-solubilizing microorganisms, and beneficial fungi, were evaluated under full and reduced nitrogen fertilization regimes. The experiment consisted of the two levels of nitrogen fertilization, and the experimental treatments where four different microbial bioagents that were a combination of several different microorganisms were applied in four different application treatments (control (without microbial bioagents), in soil, on seed, and in soil and seed). The results indicate that by reducing mineral fertilization and using microbial bioagents we can achieve twice-as-high yields compared to the sole full mineral fertilization, significantly increasing the uptake of nutrients and SOC. The uptake of toxic trace elements also increased, however, all levels remained below permissible thresholds, ensuring feed safety. The findings highlight the potential of microbial bioagents to enhance soil health, improve plant nutrition, and increase ryegrass yields while reducing reliance on synthetic inputs, contributing to climate change mitigation through improved SOC storage, and presenting a pathway for sustainable agriculture. Full article

The spread of invasive pests exacerbates the direct damage to host plants and the potential threat to the environment. Silicon has the potential to enhance host plant resistance to insects while also increasing plant yield. This study evaluated changes in Italian ryegrass biological yield and resistance to fall armyworm (Spodoptera frugiperda) larvae after silicon supplementation (sodium silicate and potassium silicate at 6 mmol·L⁻¹ were denoted as groups T1 and T2, respectively). Silicon supplementation significantly increased the shoot biological yield (T1 by 30.26%, T2 by 23.05%) and silicon content (T1 by 22.61% and T2 by 12.43%) of Italian ryegrass. At the same time, silicon supplementation increased the protein, soluble sugar, and vitamin contents of Italian ryegrass, while also stimulating the improvement of its physical and chemical defenses. Therefore, even though the nutrient intake of fall armyworm increased, the synergistic physical-chemical defense formed by silica deposition, flavonoid content, and increased protease inhibitor activity in the Italian ryegrass still weakened the antioxidant capacity of the larvae and inhibited larval feeding and protein accumulation. The larval body weight of the T1 and T2 groups decreased by 20.32% and 15.16%, respectively. The comprehensive scores showed that sodium silicate and potassium silicate of the same concentration had similar effects on the growth and insect resistance of Italian ryegrass. These findings suggest that both sodium and potassium silicate are effective silicon supplements for host plants. Therefore, reasonable supplementation of silicon fertilizer may become an important alternative plan for optimizing the comprehensive pest control strategy in agricultural production areas in the future, but this still needs further field research verification. Full article

The rising demand for climate change mitigation has brought attention to agricultural systems focused on carbon farming and reducing emissions. Composting food wastes and livestock manure not only mitigates environmental concerns but also boosts soil fertility and crop yields as an alternative fertilizer. In this experiment, we investigated the effects of different fertilizer types (chemical and organic waste compost) and crop rotations (rice–fallow, rice–Italian ryegrass, and rice–potato) on rice production, nitrogen use efficiency, and soil carbon stocks. In this experiment, soil carbon and nitrogen retention were more influenced by compost nutrient levels than by crop rotation types. Overall, as the nitrogen levels increased, the rice yields improved with both chemical and organic waste fertilizers. Among the crop rotations, the rice–Italian ryegrass rotation showed a higher nitrogen use efficiency. Optimal fertility levels, balancing nitrogen use efficiency, yield, and soil carbon were observed between 523 and 582 kg N ha⁻¹ when combined with specific crop rotations. Moreover, soil total carbon and soil total nitrogen varied among crop rotation systems. Our results indicate that organic waste compost can be a potential alternative to chemical fertilizers, while crop rotations offer a viable approach for maximizing the environmental benefits. Full article

This study aimed to assess the fermentation characteristics, bacterial community structure, co-occurrence networks, and their predicted functionality and pathogenic risk in high-moisture Italian ryegrass (IR; Lolium multiflorum Lam.) silage. The IR harvested at heading stage (208 g dry matter (DM)/kg fresh weight) was spontaneously ensiled in plastic silos (10 L scale). Triplicated silos were opened after 1, 3, 7, 15, 30, and 60 days of fermentation, respectively. The bacterial community structure on days 3 and 60 were investigated using high-throughput sequencing technology, and 16S rRNA-gene predicted functionality and phenotypes were determined by PICRUSt2 and BugBase tools, respectively. After 60 days, the IR silage exhibited good ensiling characteristics indicated by large amounts of acetic acid (~58.7 g/kg DM) and lactic acid (~91.5 g/kg DM), relatively low pH (~4.20), acceptable levels of ammonia nitrogen (~87.0 g/kg total nitrogen), and trace amounts of butyric acid (~1.59 g/kg DM). Psychrobacter was prevalent in fresh IR, and Lactobacillus became the most predominant genus after 3 and 60 days. The ensilage process reduced the complexity of the bacterial community networks in IR silage. The bacterial functional pathways in fresh and ensilaged IR are primarily characterized by the metabolism of carbohydrate and amino acid. The pyruvate kinase and 1-phosphofructokinase were critical in promoting lactic acid fermentation. A greater (p < 0.01) abundance of the “potentially pathogenic” label was noticed in the bacterial communities of ensiled IR than fresh IR. Altogether, the findings indicated that the high-moisture IR silage exhibited good ensiling characteristics, but the potential for microbial contamination and pathogens still remained after ensiling. Full article

This study examined the effects of different forage sources on the ruminal bacteriome, growth performance, and carcass characteristics of Hanwoo steers during the fattening stage. In Korea, where high-concentrate feeding is common, selecting suitable forage is crucial for sustainable beef production. Fifteen 23-month-old Hanwoo steers, weighing an average of 679.27 ± 43.60 kg, were fed the following five different forage sources: oat hay (OAT), rye silage (RYE), Italian ryegrass (IRS), barley forage (BAR), and rice straw silage (RSS), alongside 1.5 kg of dry matter concentrate daily for five months. Carcass traits were evaluated post-slaughter, and rumen fluid samples were analyzed using full-length 16S rRNA gene sequencing to determine the bacteriome composition. The forage source significantly affected the alpha-diversity indices and bacteriome biomarkers linked to the feed efficiency and ruminal fermentation. Differences in the backfat thickness and meat yield index were noted, with alpha-diversity indices correlating with carcass traits. The phylum Planctomycetota, especially the family Thermoguttaceae, was linked to nitrogen fixation in high-protein diets like IRS, while the genus Limimorpha emerged as a biomarker for the meat yield. These findings highlight the importance of forage selection during late fattening to optimize beef production, considering diet and bacteriome shifts. Full article

Italian ryegrass (Lolium multiflorum Lam.) is a persistent weed species that poses significant management challenges in key agricultural crops such as wheat, corn, cotton, and soybean. This study investigated the prevalence of resistance to ACCase inhibitor herbicides, specifically diclofop and pinoxaden, among field-collected Italian ryegrass populations. The survey revealed widespread resistance to diclofop and emerging cross-resistance to pinoxaden. To elucidate the physiological mechanism of ACCase herbicide resistance, we investigated mutations in the carboxyl-transferase (CT) domain of the ACCase enzyme, a critical region for herbicide sensitivity. Using dCAPS assays and CT domain sequencing, several known resistance-conferring mutations were detected in diclofop survivors, including I1781L, W2027C, I2041N, D2078G, and C2088R. Additionally, other mutations such as L1701M, E1874A, N1878H, G1946E/Q, V1992D, and E2039D were identified. To understand the functional role of these mutations in herbicide resistance, homology modeling was performed using AutoDock Vina for selected mutation combinations. The computational analysis revealed that all mutations and their combinations resulted in reduced binding affinity with diclofop and pinoxaden compared to the wild-type ACCase CT domain. Computational binding energy predictions indicated that the G1946E mutation and the L1701M + I1781L + E1874A + N1878H combination exhibited the lowest affinities for diclofop and pinoxaden, respectively. This study provides valuable insights into the molecular basis of ACCase inhibitor resistance in Italian ryegrass. However, further research is needed to validate the functional significance of each new substitution and its combinations in conferring herbicide resistance. Full article

In the traditional farming systems, the excessive application of chemical fertilizers to boost crop yields has resulted in a range of issues, such as soil quality degradation, soil structure deterioration, and pollution of the farmland ecological environment. Green manure, as a high-quality biological fertilizer source with rich nutrient content, is of great significance for enhancing the soil quality and establishing a healthy farmland ecosystem. However, there are few studies on the effects of different green manures on the soil nutrient levels, enzyme activities, and soil bacterial community composition in the rice–wheat rotation areas in southern China. Thus, we planted Chinese milk vetch (MV; Astragalus sinicus L.), light leaf vetch (LV; Vicia villosa var.), common vetch (CV; Vicia sativa L.), crimson clover (CC; Trifolium incarnatum L.), Italian ryegrass (RG; Lolium multiflorum L.), and winter fields without any crops as a control in the Taihu Lake area of Jiangsu. The soil samples collected after tilling and returning the green manure to the field during the bloom period were used to analyze the effects of the different green manures on the soil nutrient content, enzyme activity, and the structural composition of the bacterial community. This analysis was conducted using chemical methods and high-throughput sequencing technology. The results showed that the green manure returned to the field increased the soil pH, soil organic matter (SOM), alkali-hydrolyzed nitrogen (AN), available phosphorus (AP), available potassium (AK), sucrose (SC), urease (UE), and neutral phosphatase (NEP) contents compared to the control. They increased by 1.55% to 10.06%, 0.26% to 9.31%, 20.95% to 28.42%, 20.66% to 57.79%, 12.38% to 37.94%, 3.11% to 58.19%, 6.49% to 32.99%, and 50.0% to 80.36%, respectively. In addition, the green manure field increased the relative abundance of the genera Proteobacteria and Haliangium while decreasing the relative abundance of Gemmatimonadetes, Chloroflexi, SBR1031, and Anaeromyxobacter in the soil bacteria. Both the number of ASVs (amplicon sequence variants) and α-diversity of the soil bacterial communities were higher compared to the control, and the β-diversity varied significantly among the treatments. Alkali-hydrolyzed nitrogen and neutral phosphatase had the greatest influence on the soil bacterial community diversity, with alkali-hydrolyzed nitrogen being the primary soil factor affecting the soil bacterial community composition. Meanwhile, the results of the principal component analysis showed that the MV treatment had the most significant impact on soil improvement. Our study provides significant insights into the sustainable management of the soil quality in rice–wheat rotations. It identifies MV as the best choice among the green manure crops for improving the soil quality, offering innovative solutions for reducing chemical fertilizer dependence and promoting ecological sustainability. Full article