Search Results (21)

The emergence of mobile private networks (MPNs) has enabled tailored communication solutions for industries, enterprises, and specialized applications, fostering improved control, security, and flexibility. With the rapid advancements in software-defined radio (SDR) platforms, implementing MPNs using cost-effective and versatile hardware has become increasingly feasible. Analyzing the radio coverage of such networks is critical for optimizing performance, ensuring reliable connectivity, and addressing site-specific challenges in deployment. This paper investigates the radio coverage of a 4G MPN implemented using as radio front-end an SDR platform from the Universal Software Radio Peripheral (USRP) family and the srsRAN-4G open-source software suite. Using the HTZ Communication software as simulation tool and field-test measurements performed using an off-the-shelf mobile phone as user equipment (UE), an analysis is made to evaluate the accuracy of various propagation models in predicting network coverage, in several different frequency bands. The results provide valuable insights into the design and deployment of MPNs, highlighting the importance of accurate coverage estimation in achieving robust and efficient network operation. Full article

The aim of combining agents with different antibacterial mechanisms of action is to achieve a combined effect, which could be either the sum of their individual effects or a synergistic effect greater than the sum of these individual contributions. Mathematically, it seems reasonable to use the simple addition of agent efficacy coefficients to simplify calculations. However, this article examines the validity of this simplification in mathematical models by calculating individual and synergistic bactericidal effects using the “black box” model. All agents were characterized according to current laboratory practice. The relative antibacterial efficacy coefficients of silver nanoparticles in a colloid with chitosan succinate (nAg SCC HTZ) and graphene oxide nanoparticles (GO) were determined. In particular, the activity of silver colloid was found to be 0.29 times the bactericidal activity of erythromycin, while the activity of GO was 0.107 times the bactericidal activity of the same antibiotic against Pseudomonas aeruginosa. At the same time, all the agents demonstrated stable bacteriostatic activity and were well described by linear regression. Testing the combined effects of agents did not reveal any drug synergy. Thus, the effect of silver at a given dose, followed by the addition of GO at a bacteriostatic dose, yielded an unreliable response, different from that of the “silver–GO” system at the same simultaneous inhibition doses (p > 0.1). The data obtained can be used to develop novel combined composite materials with bactericidal properties and to predict their characteristics. Full article

With rapid advancements in the Internet of Things (IoT), Low-Power Wide-Area Networks (LPWANs) play a crucial role in expanding IoT’s capabilities while using minimal energy. Among the various LPWAN technologies, LoRaWAN (Long-Range Wide-Area Network) is particularly notable for its capacity to enable long-range, low-rate communications with low power needs. This study investigates how to optimize the placement of LoRaWAN gateways by using a combination of Particle Swarm Optimization (PSO) and Differential Evolution (DE). The approach is validated through simulations driven by HTZ to evaluate network performance in urban settings. Centered around the area of the Politehnica University of Bucharest, this research examines how different gateway placements on various floors of a building affect network coverage and packet loss. The experiment employs Adeunis Field Test Devices (FTDs) and Dragino LG308-EC25 gateways, systematically testing two spreading factors, SF7 and SF12, to assess their effectiveness in terms of signal quality and reliability. An innovative optimization algorithm, GateOpt PSODE, is introduced, which combines PSO and DE to optimize gateway placements based on real-time network performance metrics, like the Received Signal Strength Indicator (RSSI), the Signal-to-Noise Ratio (SNR), and packet loss. The findings reveal that strategically positioning gateways, especially on higher floors, significantly improves communication reliability and network efficiency, providing a solid framework for deploying LoRaWAN networks in intricate urban environments. Full article

The Saccharomyces cerevisiae chromosomal architectural protein Hmo1 is categorized as an HMGB protein, as it contains two HMGB motifs that bind DNA in a structure-specific manner. However, Hmo1 has a basic C-terminal domain (CTD) that promotes DNA bending instead of an acidic one found in a canonical HMGB protein. Hmo1 has diverse functions in genome maintenance and gene regulation. It is implicated in DNA damage tolerance (DDT) that enables DNA replication to bypass lesions on the template. Hmo1 is believed to direct DNA lesions to the error-free template switching (TS) pathway of DDT and to aid in the formation of the key TS intermediate sister chromatid junction (SCJ), but the underlying mechanisms have yet to be resolved. In this work, we used genetic and molecular biology approaches to further investigate the role of Hmo1 in DDT. We found extensive functional interactions of Hmo1 with components of the genome integrity network in cellular response to the genotoxin methyl methanesulfonate (MMS), implicating Hmo1 in the execution or regulation of homology-directed DNA repair, replication-coupled chromatin assembly, and the DNA damage checkpoint. Notably, our data pointed to a role for Hmo1 in directing SCJ to the nuclease-mediated resolution pathway instead of the helicase/topoisomerase mediated dissolution pathway for processing/removal. They also suggested that Hmo1 modulates both the recycling of parental histones and the deposition of newly synthesized histones on nascent DNA at the replication fork to ensure proper chromatin formation. We found evidence that Hmo1 counteracts the function of histone H2A variant H2A.Z (Htz1 in yeast) in DDT possibly due to their opposing effects on DNA resection. We showed that Hmo1 promotes DNA negative supercoiling as a proxy of chromatin structure and MMS-induced DNA damage checkpoint signaling, which is independent of the CTD of Hmo1. Moreover, we obtained evidence indicating that whether the CTD of Hmo1 contributes to its function in DDT is dependent on the host’s genetic background. Taken together, our findings demonstrated that Hmo1 can contribute to, or regulate, multiple processes of DDT via different mechanisms. Full article

Combining ionic liquids (ILs) and metal–organic frameworks (MOFs) can be an intriguing opportunity to develop advanced materials with different adsorption capabilities for environmental applications. This study reports the preparation and characterization of a 3D pillared-layered compound, namely, [Zn₂(tz)₂(bdc)] (CIM91), formed by 1,2,4-triazole (Htz) and 1,4-benzenedicarboxylic acid (H₂bdc) ligands. Then, various loadings of the water-stable and hydrophobic IL, 1-n-butyl-3-methylimidazolium hexafluorophosphate ([BMIM][PF₆]), and the water-soluble 1-n-butyl-3-methylimidazolium chloride ([BMIM][Cl]) were incorporated into CIM91. Detailed characterization by X-ray powder diffraction (XRD), FT-IR spectra, scanning electron microscopy (SEM), Energy dispersive X-ray (EDX) analysis, N₂ adsorption measurements, and thermogravimetric analysis confirmed the formation of [BMIM][X]/CIM91 composites and the structural stability of the MOF after the incorporation of the ionic liquids. CO₂ adsorption–desorption analysis was experimentally carried out for all the materials at 298 K and 318 K, demonstrating a great enhancement in the CO₂ adsorption properties of the sole MOF CIM91, particularly by including [BMIM][PF₆] species in its structure with a double isosteric heat of CO₂ adsorption. The composites were also tested for the adsorption of methylene blue (MB) dye. The results indicate that the incorporation of [BMIM][X] into CIM91 can substantially modify the adsorption properties of the MOF. The influence of the nature of the [BMIM][X] anions on these properties has also been analyzed. Full article

Fixed-dose combination therapy is considered a practical approach in the treatment of various diseases, as it can simultaneously target different mechanisms of action that achieve the required therapeutic efficacy through a synergistic effect. A combination of hydrochlorothiazide (HTZ), amlodipine (AMD), and valsartan (VLS) has been created for the treatment of hypertension. Therefore, the aim of this study was to develop an optimized UPLC method for the simultaneous quantification of this combination. A DoE at a level of 32 was used to investigate the effects of column temperature (20, 30, and 40 °C) and formic acid concentration (0.05, 0.15, and 0.25%) on the retention time of each active pharmaceutical ingredient (API), the peak area, and the peak symmetry, as well as the resolution between HTZ-AMD and AMD-VLS peaks. The optimized analytical method was validated and used to extract the three APIs from the marketed product. The optimized analytical condition with a column temperature of 27.86 °C and a formic acid concentration of 0.172% showed good separation of the three APIs in 1.62 ± 0.006, 3.59 ± 0.002, and 3.94 ± 0.002 min for HTZ, AMD, and VST, respectively. The developed method was linear with the LOQ for a HTC, AMD, and VST of 0.028, 0.038, and 0.101 ppm, respectively. Moreover, the developed assay was sustainable and robust, with an RSD % of less than 2%. The application of this method in the extraction of HTZ, AMD, and VST from the Exforge^® marketed product showed good separation with a measurable drug content of 23.5 ± 0.7, 9.68 ± 0.1, and 165.2 ± 5.2 mg compared to the label claims of 25/10/160 for HTZ, AMD, and VST, respectively. Full article

The main issue in the development of precise positioning is the way in which to send GNSS corrections. To handle this case, a terrestrial broadcast mechanism based on DAB+ (digital audio broadcasting) signals was chosen to overcome the scalability challenges, as it would allow a service provider to transfer assistance data based on a “one-to-many” scheme. DAB is easily widely deployed, with large coverage predictions carried using ATDI’s HTZ simulator. The DAB+ reception demonstrator uses SSRZ, a compressed and compact state space representation corrections format developed by Geo++. SSRZ corrections were generated using data from Teria’s CORS network and have been broadcasted over the air with DAB+ signals. To validate the SSRZ corrections, the “Record&Replay” technique was used. It consists of digitizing GNSS signals during a normal field test. Once data were collected and validated, replays were executed in the laboratory. Different scenarios were set up to validate the accuracy of the position with SSRZ corrections sent via DAB+ and the convergence to the corrected position after ambiguity loss. The results show that the accuracy was acceptable when the user remained close to the transmitting DAB+ antenna (<10 km). The lower performance might result from broadcast limitations in urban environments. Full article

The reaction of CF₃COOAg, 3-bdppmapy (N,N-bis(diphenylphosphanylmethyl)-3-aminopyridine) and HTZ (1,2,4-triazole-3-thiol) in CH₂Cl₂/MeOH resulted in a dinuclear Ag/P/S complex [Ag₂(TZ)₂(3-bdppmapy)₂]·xSol (1·xSol). Crystals of 1·xSol converted to 1·2MeOH in air at room temperature and further to 1 under vacuum upon heating. The solid-state, room-temperature photoluminescent emission of 1·xSol (510 nm) shifted to 494 nm (1·2MeOH) and 486 nm (1). Grinding solids of 1·2MeOH in air resulted in amorphous 1G characterized by solid-state emission at 468 nm, which converted to 1GR with 513 nm emission upon MeOH treatment. Grinding 1GR in air returned 1G, and this interconversion was reproducible over five cycles. The solid-state photoluminescence of 1G changed in response to vapors containing low–molecular weight alcohols but remained unchanged after exposure to other volatile organic compounds (VOCs) or to water vapor. Test papers impregnated with 1G could detect methanol in vapors from aqueous solutions at concentrations above 50%. Complex 1G is, therefore, an example of a stimuli-responsive molecular sensor for the detection of alcohols. Full article

Hydrochlorothiazide (HTZ) and Valsartan (VAL) are poorly soluble drugs in BCS classes IV and II. This study aimed to develop a method to assess the dissolution profile of tablets containing HTZ (12.5 mg) and VAL (160 mg) as a fixed-dose combination, using in silico tools to evaluate products marketed in Brazil and Peru. Firstly, in vitro dissolution tests were performed using a fractional factorial design 3³⁻¹. Then, DDDPlus™ was used to carry out experimental design assays of a complete factorial design 3³. Data from the first stage were used to obtain calibration constants for in silico simulations. The factors used in both designs were formulation, sinker use, and rotation speed. Finally, effects and factor interaction assessment was evaluated based on a statistical analysis of the dissolution efficiency (DE) obtained from simulations. Thus, the established final conditions of the dissolution method were 900 mL of phosphate buffer pH 6.8, 75 rpm of rotation speed, and sinker use to prevent formulation floating. The reference product stood out because of its higher DE than other formulations. It was concluded that the proposed method, in addition to ensuring total HTZ and VAL release from formulations, has adequate discriminative power. Full article

With the increasingly serious environmental problems, coordinating the relationship between the environment and economic development has become a crucial task for developing countries, especially China. This paper studies the role of eco-industrial parks (EIPs) in the emissions of carbon dioxide and sulfur dioxide in China with the difference-in-difference (DID) approach by focusing on the entire process of EIPs’ establishment—upgrading provincial development zones (DZs) to national DZs and then transforming national DZs into EIPs. Besides, we examined the heterogeneous effect of the different transformations from national economic and technological development zones (ETZs) or national high-tech zones (HTZs) to EIPs. In addition, we studied the spatial spillover effects of EIPs and their paths with the spatial difference-in-difference (SDID) method. The results show that neither provincial DZs nor national DZs can significantly reduce sulfur dioxide and carbon dioxide emissions. Only when national DZs are transformed into EIPs can they be reduced significantly. However, the different transformations from the HTZs and ETZs to EIPs have different effects on emissions. Moreover, EIPs have technology spillovers and demonstration effects on surrounding areas. Therefore, EIPs can reduce emissions in the surrounding areas. The results indicate that, in order to achieve high-quality development and coordinate the relationship between environment and economic development, we should take positive steps to promote the transformation of DZs into EIPs. Full article

Environmental pollution has recently become a serious economic issue, and finding ways to reduce pollution in economic development is an urgent task for developing countries, especially China. In this paper, we aim to document the policy role of development zones (DZs) in promoting China’s economic development on environmental pollution and consider the establishment of DZs as a quasi-natural experiment. Specifically, we identify the establishment of DZs on pollution emissions based on the staggered difference-in-difference (DID) approach by setting a dummy variable for DZ policies. Furthermore, we examine the heterogeneity of provincial and national (high-tech and economic development zones) DZs on pollution to detect the government domination effect. Finally, in order to deal with the potential spatial spillover effects of DZs, this paper applies the spatial difference-in-difference (SDID) method to explore the spatial reallocation effects of DZs. The results indicate that the provincial DZs can aggravate China’s pollution intensity, but they will no longer play the same role for national-level policies. Moreover, we find that national high-tech industrial DZs (HTZs) can reduce pollution intensity. In particular, the national DZs can bring about the reallocation of pollution among cities working as a selective place-based policy. That means that the national HTZs will not only reduce the local pollution intensity, but also reduce that of surrounding areas. Our empirical results highlight that cities should be encouraged to set up national HTZs in order to achieve an environmentally friendly high-quality development goal. Full article

China’s High-tech Industrial Development Zones (HTZ) are industrial agglomeration areas established by the local government to foster economic innovation. As springboards for cities to implement innovation-driven development strategies, HTZs have significant spillover and driving effects on urban ecological innovation. Based on panel data taken from 215 cities between 2003 and 2016, this paper empirically analyzes the impact of HTZ construction and its mechanisms as they pertain to urban ecological innovation. This analysis is framed by the double difference model and the intermediary effect model. It found that HTZ construction can effectively enhance urban ecological innovation, and formidably promote ecological innovation in central and eastern cities, as well as cities with superior scientific and educational resources. The intermediary mechanism analysis revealed that HTZs result in a policy depression effect, which may promote the agglomeration of urban innovation factors (including high-quality talents and investment), thereby bolstering urban ecological innovation. Moreover, HTZs’ investment agglomeration effect is primarily responsible for driving urban ecological innovation. Indeed, the HTZ construction may not only promote the local ecological innovation, but also have a significant spillover effect on the ecological innovation activities of other cities in the province. Full article

The mechanochemical synthesis of drug–drug solid forms containing metformin hydrochloride (MET·HCl) and thiazide diuretics hydrochlorothiazide (HTZ) or chlorothiazide (CTZ) is reported. Characterization of these new systems indicates formation of binary eutectic conglomerates, i.e., drug–drug eutectic solids (DDESs). Further analysis by construction of binary diagrams (DSC screening) exhibited the characteristic V-shaped form indicating formation of DDESs in both cases. These new DDESs were further characterized by different techniques, including thermal analysis (DSC), solid state NMR spectroscopy (SSNMR), powder X-ray diffraction (PXRD) and scanning electron microscopy–energy dispersive X-ray spectroscopy analysis (SEM–EDS). In addition, intrinsic dissolution rate experiments and solubility assays were performed. In the case of MET·HCl-HTZ (χ_MET·HCl = 0.66), we observed a slight enhancement in the dissolution properties compared with pure HTZ (1.21-fold). The same analysis for the solid forms of MET·HCl-CTZ (χ_MET·HCl = 0.33 and 0.5) showed an enhancement in the dissolved amount of CTZ accompanied by a slight improvement in solubility. From these dissolution profiles and saturation solubility studies and by comparing the thermodynamic parameters (ΔH_fus and ΔS_fus) of the pure drugs with these new solid forms, it can be observed that there was a limited modification in these properties, not modifying the free energy of the solution (ΔG) and thus not allowing an improvement in the dissolution and solubility properties of these solid forms. Full article

Background: currently applied surface treatments for zirconia bonding may create undesired microcracks and surface flaws. The aim of the present study was to evaluate the efficacy of alternative surface treatments on the shear bond strength of high translucency zirconia to 10-Methacryloyloxydecyl dihydrogen phosphate (MDP)-containing resin-based cement. Methods: fifty disk-shaped specimens (10 mm × 5 mm) were fabricated from a commercial yttria-stabilized zirconia with 5 mole% yttrium oxide tetragonal zirconia polycrystal (5Y-TZP), and underwent air-abrasion with alumina particles (50 μm-AL50 and 90 μm-AL90), glass beads (GB 10–60 μm), and ablation with femtosecond laser (FEMTO). Shear bond strength was evaluated with a universal testing machine under a crosshead speed of 0.5 mm/min until fracture. Fracture type was evaluated with an optical stereomicroscope. Differences among groups were evaluated by one-way ANOVA and Bonferroni pairwise comparison tests (p < 0.05). Results: the highest shear bond strength values were presented by the laser treated group (23.97 ± 3.7 MPa). No statistically significant differences were found among the Cl, Al50, Al90 and FEMTO groups. The lowest mean value was presented by the glass-beads treated group (11.93 ± 2.88 MPa) which was significantly lower compared to all other groups (p < 0.001). Conclusions: under the limitations of this in vitro study, femtosecond laser treatment of High-translucent monolithic zirconia (HTZ) ceramics is a promising alternative method for the mechanical retention of resin cements. Full article

Pretargeted nuclear imaging for the diagnosis of various cancers is an emerging and fast developing field. The tetrazine ligation is currently considered the most promising reaction in this respect. Monoclonal antibodies are often the preferred choice as pretargeting vector due to their outstanding targeting properties. In this work, we evaluated the performance of [⁶⁴Cu]Cu-NOTA-PEG₇-H-Tz using a setup we previously used for [¹¹¹In]In-DOTA-PEG₁₁-BisPy-Tz, thereby allowing for comparison of the performance of these two promising pretargeting imaging agents. The evaluation included a comparison of the physicochemical properties of the compounds and their performance in an ex vivo blocking assay. Finally, [⁶⁴Cu]Cu-NOTA-PEG₇-H-Tz was evaluated in a pretargeted imaging study and compared to [¹¹¹In]In-DOTA-PEG₁₁-BisPy-Tz. Despite minor differences, this study indicated that both evaluated tetrazines are equally suited for pretargeted imaging. Full article