Search Results (6)

Prolonged cultivation of cereal-based cropping systems in the Indo-Gangetic Plain has contributed to soil degradation, groundwater depletion, and declining soil organic carbon levels, highlighting the urgent need for climate-resilient, sustainable crop diversification strategies that enhance soil carbon sequestration and improve overall soil health. A 6-year field experiment assessed 10 cropping systems (CSs) using a randomized complete block design with four replications, focusing on their effects on soil carbon stocks and sequestration at two soil depths (0–15 cm and 15–30 cm). It was inferred from the results that there is a significant variation in soil carbon stocks, with maize–peas–spring groundnut (CS6) having the highest surface carbon stock (13.0 Mg ha⁻¹) and baby corn–potato–okra (CS10) having the highest sub-surface carbon stock (11.9 Mg ha⁻¹). Carbon sequestration peaked in CS6 at 5.06 Mg ha⁻¹ at 0–15 cm, and its sequestration rate was the highest (0.84 Mg ha⁻¹ yr⁻¹). Total organic carbon (TOC) ranged from 0.63% in Rice–Wheat (CS1) to 0.73% in CS6, with similarly high values in other diversified systems. Very labile carbon (VLC) was highest in basmati rice, late-sown wheat, and cowpea (CS3) and CS6, demonstrating the benefits of legume-based systems. At depths of 15–30 cm, trends were consistent but lower. Water-soluble carbon (WSC) and hot water-soluble carbon (HWSC) showed significant differences across systems, with CS3 recording the highest values. The findings indicate that cropping systems incorporating legume diversification and green manuring enhance carbon stocks, sequestration rates, and soil carbon stability, demonstrating that crop diversification is an effective means of increasing soil carbon storage, promoting soil health, and supporting sustainable agricultural production in Northwestern India. Full article

Transportation, environmental changes, and overcrowding can induce short-term stress in livestock, leading to physiological imbalances even within a short period. Cortisol is a stress-response hormone and its concentration in the blood can rapidly fluctuate depending on the individual and situation. This study evaluated the short-term effects of cortisol by applying blood cortisol concentrations that mimicked the normal and stress-induced levels observed in Korean native cattle (Hanwoo) to the culture medium of Hanwoo muscle stem cells (HWSC). Treatments were designed with five cortisol concentrations (0, 5, 10, 30, and 70 ng/mL) and four treatment times (0.5, 1, 2, and 3 h), based on the CCK-8 and viable cell count results. The expression levels of cortisol receptor-related genes (NR3C1, HSP70, and HSP90AA1) increased and reached a peak at 30 min post-treatment. After 30 min, the expression of these genes gradually decreased. However, in the case of HSP70, expression tended to increase again after 3 h of treatment. This could be seen as the regulation of cortisol inflow into the HWSC. Upon examining the oxidative effects of cortisol on superoxide dismutase 1 (SOD1), glutathione peroxidase (GPX), catalase (CAT), and oxygen consumption rate (OCR), the expression of antioxidant factors increased and peaked at 30 min of treatment. Following this peak, their levels generally began to decrease. However, in the 70 ng/mL group, the expression of these factors remained at a high level compared to the control group even after 30 min. In addition, the cellular respiration index and ATP production increased as the treatment prolonged, regardless of the concentration, as shown by the OCR analysis. These results can be considered a phenomenon corresponding to the accumulation of oxidative by products, such as Reactive Oxygen Species (ROS), caused by cortisol. The gene expression of apoptosis factors (p53, BAX, Caspase-3) temporarily increased at 30 min but then decreased. Caspase-3 protein activity was elevated at 30 min in the 70 ng/mL group, which later reduced. These results suggested that short-term cortisol administration had no effect on apoptosis in muscle cell culture. Therefore, the study findings elucidating the effects of short-term cortisol treatment on HWSC suggest that short-term stress may not have a significant negative effect on Hanwoo muscle. However, as this study was limited to muscle stem cells derived from Hanwoo, further investigation is required to determine whether the observed responses are consistent across different species and in vivo environments. Full article

The principles of myogenesis play crucial roles in the production of cultured meat, and identifying protein stimulators associated with myogenesis holds great potential to enhance the efficiency of this process. In this study, we used surface plasmon resonance (SPR)-based screening of a natural product library to discover ligands for Pax7 and MyoD, key regulators of satellite cells (SCs), and performed cell-based assays on Hanwoo SCs (HWSCs) to identify substances that promote cell proliferation and/or differentiation. Through an SPR analysis, we found that six chemicals, including one Pax7+/MyoD− chemical, four Pax7+/MyoD+ chemicals, and one Pax7−/MyoD+ chemical, bound to Pax7 and/or MyoD proteins. Among four Pax7+/MyoD+ chemicals, parthenolide (0.5 and 1 µM) and rutin (100 and 200 µM) stimulated cell proliferation in the medium with 10% FBS similar to the medium with 20% FBS, without affecting differentiation. Adenosine, a Pax7−/MyoD+ chemical, accelerated differentiation. These chemicals could be potential additives to reduce the reliance of FBS required for HWSC proliferation and differentiation in cultured meat production. Full article

Harvest weed seed control (HWSC) methods destroy, remove, or concentrate weed seeds collected during harvest. Depending on the method of HWSC, chaff and straw fractions may also be destroyed, removed, or concentrated. Observations at soybean (Glycine max (L.) Merr.) harvest in this study estimated the distribution of aboveground biomass between seed, straw, and chaff fractions and the nutrient composition of straw and chaff. Measurements were combined to predict nutrient consequences of HWSC, which have not been documented. The average harvest index of soybean was 0.57:1. Soybean biomass that enters the combine partitions into 7.25 $\pm$ 0.37% chaff, 36.05 $\pm$ 1.2% straw, and 56.7 $\pm$ 1.2% seed. Chaff and straw residues equal 13.4% and 68.5% of the seed weight, respectively. In a soybean crop yielding 3368 kg ha⁻¹ (50 bu a⁻¹), chaff yields 9.4, 0.8, 5.0, and 0.6 kg ha⁻¹ and straw 31.6, 2.1, 1.1, and 2.0 kg ha⁻¹ of N, P, K, and S, respectively. Using 5-year average fertilizer prices ending in 2021, the cost to replace chaff, straw, and the combination of both residues is USD 1.58, USD 5.88, and USD 7.46, respectively. These results give insight into the nutrient consequences and replacement costs of HWSC. Full article

If seeds retained on weeds at crop harvest could be collected and removed by the combine harvester, weed infestation could be reduced in the following years. We estimated the proportion of weed seeds that could be removed at oat harvest. The seed production and shedding pattern of Fallopia convolvulus, Sinapis arvensis, Spergula arvensis and Stellaria media, were assessed in two spring oat fields in Denmark during 2018 and 2019. Ten randomly chosen plants of each species were surrounded by a porous net before flowering. The start time of seed shedding was recorded, and the seeds were collected from the nets and counted weekly until oat harvest. Just before harvest, the retained seeds on the weed plants were counted. The ratio between harvestable seeds and shed seeds during the growing season was determined. On average 260, 195, 411 and 316 seeds plant⁻¹ were produced by F. convolvulus, Sinapis arvensis, Spergula arvensis and S. media, respectively, of which in average 44%, 67%, 45% and 56% of the seeds were retained on the plants at harvest. There was a strong, positive correlation between the weed biomass and the total seed production. Full article

During harvesting, grain, straw, and chaff with weed seeds are separated. The chaff is returned to the fields, resulting in weed problems in the subsequent crops. We estimated the fraction of weed seeds a combine harvester could potentially harvest and used various methods to collect the chaff and treat it with heat to kill weed seeds or reduce weed seed germination. Chaff with weed seeds was placed on top of the straw and afterwards baled with the straw as a method to remove weed seeds from the field. We exposed chaff with weed seeds to exhaust gas with various temperatures and durations to study whether this heating method could be used to reduce the input of viable weed seeds to the soil during harvesting. By collecting the shed weed seeds during the growing season, we estimated that a combine harvester could potentially harvest 41%, 11%, and 100% of the seeds produced in the growing season by Bromus hordeaceus, Cirsium arvense, and Galium aparine, respectively. When the chaff was placed on top of the straw, 45% of the weed seeds stayed in the chaff fraction on top of the straw swath after one day, 35% got into the straw swath, and 20% past through the swath to the ground. Therefore, baling straw with chaff placed on the top only had a limited effect on reducing weed seed infestation. The study showed that thermal weed seed control during harvesting could potentially be applicable and incorporated in an integrated weed management approach. Full article