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23 pages, 18349 KiB  
Article
Estimating Radicle Length of Germinating Elm Seeds via Deep Learning
by Dantong Li, Yang Luo, Hua Xue and Guodong Sun
Sensors 2025, 25(16), 5024; https://doi.org/10.3390/s25165024 - 13 Aug 2025
Viewed by 219
Abstract
Accurate measurement of seedling traits is essential for plant phenotyping, particularly in understanding growth dynamics and stress responses. Elm trees (Ulmus spp.), ecologically and economically significant, pose unique challenges due to their curved seedling morphology. Traditional manual measurement methods are time-consuming, prone [...] Read more.
Accurate measurement of seedling traits is essential for plant phenotyping, particularly in understanding growth dynamics and stress responses. Elm trees (Ulmus spp.), ecologically and economically significant, pose unique challenges due to their curved seedling morphology. Traditional manual measurement methods are time-consuming, prone to human error, and often lack consistency. Moreover, automated approaches remain limited and often fail to accurately process seedlings with nonlinear or curved morphologies. In this study, we introduce GLEN, a deep learning-based model for detecting germinating elm seeds and accurately estimating their lengths of germinating structures. It leverages a dual-path architecture that combines pixel-level spatial features with instance-level semantic information, enabling robust measurement of curved radicles. To support training, we construct GermElmData, a curated dataset of annotated elm seedling images, and introduce a novel synthetic data generation pipeline that produces high-fidelity, morphologically diverse germination images. This reduces the dependence on extensive manual annotations and improves model generalization. Experimental results demonstrate that GLEN achieves an estimation error on the order of millimeters, outperforming existing models. Beyond quantifying germinating elm seeds, the architectural design and data augmentation strategies in GLEN offer a scalable framework for morphological quantification in both plant phenotyping and broader biomedical imaging domains. Full article
(This article belongs to the Section Intelligent Sensors)
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22 pages, 9411 KiB  
Article
A Spatiotemporal Multi-Model Ensemble Framework for Urban Multimodal Traffic Flow Prediction
by Zhenkai Wang and Lujin Hu
ISPRS Int. J. Geo-Inf. 2025, 14(8), 308; https://doi.org/10.3390/ijgi14080308 - 10 Aug 2025
Viewed by 630
Abstract
Urban multimodal travel trajectory prediction is a core challenge in Intelligent Transportation Systems (ITSs). It requires modeling both spatiotemporal dependencies and dynamic interactions among different travel modes such as taxi, bike-sharing, and buses. To address the limitations of existing methods in capturing these [...] Read more.
Urban multimodal travel trajectory prediction is a core challenge in Intelligent Transportation Systems (ITSs). It requires modeling both spatiotemporal dependencies and dynamic interactions among different travel modes such as taxi, bike-sharing, and buses. To address the limitations of existing methods in capturing these diverse trajectory characteristics, we propose a spatiotemporal multi-model ensemble framework, which is an ensemble model called GLEN (GCN and LSTM Ensemble Network). Firstly, the trajectory feature adaptive driven model selection mechanism classifies trajectories into dynamic travel and fixed-route scenarios. Secondly, we use a Graph Convolutional Network (GCN) to capture dynamic travel patterns and Long Short-Term Memory (LSTM) network to model fixed-route patterns. Subsequently the outputs of these models are dynamically weighted, integrated, and fused over a spatiotemporal grid to produce accurate forecasts of urban total traffic flow at multiple future time steps. Finally, experimental validation using Beijing’s Chaoyang district datasets demonstrates that our framework effectively captures spatiotemporal and interactive characteristics between multimodal travel trajectories and outperforms mainstream baselines, thereby offering robust support for urban traffic management and planning. Full article
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17 pages, 12483 KiB  
Article
Southeast Asia’s Extreme Precipitation Response to Solar Radiation Management with GLENS Simulations
by Heri Kuswanto, Fatkhurokhman Fauzi, Brina Miftahurrohmah, Mou Leong Tan and Hong Xuan Do
Atmosphere 2025, 16(6), 725; https://doi.org/10.3390/atmos16060725 - 15 Jun 2025
Viewed by 783
Abstract
This study evaluates the impacts of Solar Radiation Management (SRM) on precipitation-related climate extremes in Southeast Asia. Using simulations from the Geoengineering Large Ensemble (GLENS), we assess spatial anomalies and differences in extreme precipitation indices—number of wet days (RR1), very heavy precipitation days [...] Read more.
This study evaluates the impacts of Solar Radiation Management (SRM) on precipitation-related climate extremes in Southeast Asia. Using simulations from the Geoengineering Large Ensemble (GLENS), we assess spatial anomalies and differences in extreme precipitation indices—number of wet days (RR1), very heavy precipitation days (R20mm), maximum 5-day precipitation (Rx5day), consecutive dry days (CDD), and consecutive wet days (CWD)—relative to historical (1980–2009) and Representative Concentration Pathway 8.5 (RCP8.5) baselines. The results reveal that SRM induces highly heterogeneous precipitation responses across the region. While SRM increases rainfall frequency in parts of Indonesia, it reduces the number of wet days and lengthens dry spells over Vietnam, Thailand, and the Philippines. Spatial variations are also observed in changes to heavy precipitation days and multi-day rainfall events, with potential implications for flood and drought risks. These findings highlight the complex trade-offs in hydrological responses under SRM deployment, with important considerations for agriculture, water resource management, and climate adaptation strategies in Southeast Asia. Full article
(This article belongs to the Section Climatology)
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25 pages, 3308 KiB  
Article
Nutritionally Important Pro-Health Active Ingredients and Antioxidant Properties of Fruits and Fruit Juice of Selected Biennial Fruiting Rubus idaeus L. Cultivars
by Mirosława Chwil, Renata Matraszek-Gawron, Mikołaj Kostryco and Monika Różańska-Boczula
Pharmaceuticals 2023, 16(12), 1698; https://doi.org/10.3390/ph16121698 - 7 Dec 2023
Cited by 5 | Viewed by 1781
Abstract
Raspberry fruits are an important source of many biologically active chemical compounds exerting nutritional and pro-health effects. The study presents a comparative analysis of nutritionally important bioactive chemical compounds—polyphenols; flavonoids, including anthocyanins; vitamin C; amino acids; fatty acids; and primary metabolites—contained in the [...] Read more.
Raspberry fruits are an important source of many biologically active chemical compounds exerting nutritional and pro-health effects. The study presents a comparative analysis of nutritionally important bioactive chemical compounds—polyphenols; flavonoids, including anthocyanins; vitamin C; amino acids; fatty acids; and primary metabolites—contained in the fruits of three biennial fruiting cultivars, R. idaeus ‘Glen Ample’, ‘Laszka’, and ‘Radziejowa’, i.e., common cultivars in Poland and Europe. The antioxidant activity of fresh fruits and juice was determined with five methods. The analyses revealed the strong free radical scavenging potential of the fruits and juice, confirmed by the high concentration of nutrients, e.g., polyphenols, anthocyanins, vitamin C, amino acids, and fatty acids. The antioxidant activity of the juice determined with the ferric reducing antioxidant power (FRAP) and OH radical methods was from 2.5 to 4.0 times higher than that of the fruits. The following orders of total polyphenol contents were established in the analyzed cultivars: ‘Glen Ample’ < ‘Laszka’ < ‘Radziejowa’ in the fruits and ‘Glen Ample’ < ‘Radziejowa’ < ‘Laszka’ in the juice. The highest antioxidant activity was exhibited by the ‘Radziejowa’ fruits. Given their high content of dietary fiber, the fruits of the analyzed raspberry cultivars can be consumed by dieting subjects. The concentrations of vitamin C (28–34 mg/100 g) and anthocyanins (20–34 mg/100 g) indicate the biological and pharmacological activity of these fruits. The main unsaturated fatty acids in the fruits were gamma-linoleic acid (C18:2n6c) and alpha-linolenic acid (C18:3n3), which neutralize excess free radicals. The amino acids nutritionally essential to humans were dominated by leucine, arginine, and phenylalanine. This is the first comparative analysis of the antioxidant activity of fruits and juice and the contents of selected active compounds in the fruits of biennial fruiting cultivars of R. idaeus, i.e., a highly commercialized crop in Europe. Full article
(This article belongs to the Section Natural Products)
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17 pages, 1286 KiB  
Article
Energy Management and Environmental Protection in Industrial Parks: A Comparative Study of Central Taiwan Science Park and Silicon Glen
by Fu-Hsuan Chen and Hao-Ren Liu
Buildings 2023, 13(9), 2299; https://doi.org/10.3390/buildings13092299 - 9 Sep 2023
Cited by 3 | Viewed by 2720
Abstract
This manuscript focuses on analyzing the growth dynamics of the Central Taiwan Science Park (CTSP) and Silicon Glen in Scotland with a specific emphasis on their approaches to energy, environmental conservation, and economic management. The objective is to provide insights into their sustainable [...] Read more.
This manuscript focuses on analyzing the growth dynamics of the Central Taiwan Science Park (CTSP) and Silicon Glen in Scotland with a specific emphasis on their approaches to energy, environmental conservation, and economic management. The objective is to provide insights into their sustainable development strategies. In terms of energy, CTSP addresses Taiwan’s energy security and green transformation challenges, while Silicon Glen concentrates on Scotland’s wind energy generation technologies. Both regions prioritize the advancement of renewable energy sources and smart grid technologies. In the realm of environmental conservation, both CTSP and Silicon Glen prioritize environmental protection and sustainability by implementing rigorous environmental monitoring measures. Regarding economic management, CTSP and Silicon Glen serve as vital technology industry hubs in Taiwan and Scotland, respectively, attracting a multitude of high-tech and startup enterprises. This growth is facilitated through various means, including policy support, access to research resources, and robust infrastructure. This manuscript presents a comparative analysis of these two industrial parks, focusing on their environmental and economic management strategies. It aims to elucidate the principles underpinning the sustainable development and economic growth of industrial parks, offering valuable insights to decision-makers and stakeholders involved in the planning of sustainable industrial parks. Full article
(This article belongs to the Collection Strategies for Sustainable Urban Development)
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16 pages, 4597 KiB  
Article
Application of Starch Based Coatings as a Sustainable Solution to Preserve and Decipher the Charred Documents
by Sonali Kesarwani, Divya Bajpai Tripathy and Suneet Kumar
Coatings 2023, 13(9), 1521; https://doi.org/10.3390/coatings13091521 - 30 Aug 2023
Cited by 3 | Viewed by 2626
Abstract
Fire can be one of the most destructive elements to cause devastation. Fire can completely or partly destroy any crucial and invaluable documents, such as banknotes, books, affidavits, etc., in a couple of minutes. Moreover, the documents can also be damaged by heat, [...] Read more.
Fire can be one of the most destructive elements to cause devastation. Fire can completely or partly destroy any crucial and invaluable documents, such as banknotes, books, affidavits, etc., in a couple of minutes. Moreover, the documents can also be damaged by heat, smoke, soot, and water during an accident. The burnt documents become fragile, losing their identity, which may have some evidentiary value related to the incident. Therefore, there is a strong need for processing to procure, preserve, and decipher, i.e., to restore the texts written on them. Hence, the present research focuses on developing a new method using natural polysaccharides, i.e., starch, to preserve and decipher the contents of charred documents. The most suitable concentration of starch analog was found to be 6% microwaved at 80 °C for about 10 min. As soon as the charred documents were coated with 6% starch analog, the majority of the invisible texts became visible to the naked eye in a second. Moreover, the application of a synthesized analog of polysaccharide on fragile charred documents provided an appreciable increase in strength by almost 0.1 kg/cm2 for the coated charred documents of each paper type compared to that of non-coated ones and made them stabilized. This research also involves the use of easy and advanced handwriting recognition techniques (HCR) using an easily accessible, free platform, G-lens, that successfully recognized the majority of texts deciphered using 6% starch analog and converted them from captured images to a readable and copyable text format. Furthermore, the document visualization under VSC also gave a promising result by enhancing and deciphering the non-visible and less visible texts under flood light and white spot light at 715 and 695 long passes. Hence, this study offers an environmentally friendly, cost-effective, and sustainable approach of using a natural polysaccharide instead of synthetic polymers for the preservation and decipherment of charred documents. Full article
(This article belongs to the Special Issue Advanced Coating Material for Heritage Preservation)
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38 pages, 12514 KiB  
Article
Manganese-Iron Phosphate Nodules at the Groken Site, Gale Crater, Mars
by Allan H. Treiman, Nina L. Lanza, Scott VanBommel, Jeff Berger, Roger Wiens, Thomas Bristow, Jeffrey Johnson, Melissa Rice, Reginald Hart, Amy McAdam, Patrick Gasda, Pierre-Yves Meslin, Albert Yen, Amy J. Williams, Ashwin Vasavada, David Vaniman, Valerie Tu, Michael Thorpe, Elizabeth D. Swanner, Christina Seeger, Susanne P. Schwenzer, Susanne Schröder, Elizabeth Rampe, William Rapin, Silas J. Ralston, Tanya Peretyazhko, Horton Newsom, Richard V. Morris, Douglas Ming, Matteo Loche, Stéphane Le Mouélic, Christopher House, Robert Hazen, John P. Grotzinger, Ralf Gellert, Olivier Gasnault, Woodward W. Fischer, Ari Essunfeld, Robert T. Downs, Gordon W. Downs, Erwin Dehouck, Laura J. Crossey, Agnes Cousin, Jade M. Comellas, Joanna V. Clark, Benton Clark, Steve Chipera, Gwenaël Caravaca, John Bridges, David F. Blake and Ryan Andersonadd Show full author list remove Hide full author list
Minerals 2023, 13(9), 1122; https://doi.org/10.3390/min13091122 - 25 Aug 2023
Cited by 9 | Viewed by 3570
Abstract
The MSL Curiosity rover investigated dark, Mn-P-enriched nodules in shallow lacustrine/fluvial sediments at the Groken site in Glen Torridon, Gale Crater, Mars. Applying all relevant information from the rover, the nodules are interpreted as pseudomorphs after original crystals of vivianite, (Fe2+,Mn [...] Read more.
The MSL Curiosity rover investigated dark, Mn-P-enriched nodules in shallow lacustrine/fluvial sediments at the Groken site in Glen Torridon, Gale Crater, Mars. Applying all relevant information from the rover, the nodules are interpreted as pseudomorphs after original crystals of vivianite, (Fe2+,Mn2+)3(PO4)2·8H2O, that cemented the sediment soon after deposition. The nodules appear to have flat faces and linear boundaries and stand above the surrounding siltstone. ChemCam LIBS (laser-induced breakdown spectrometry) shows that the nodules have MnO abundances approximately twenty times those of the surrounding siltstone matrix, contain little CaO, and have SiO2 and Al2O3 abundances similar to those of the siltstone. A deconvolution of APXS analyses of nodule-bearing targets, interpreted here as representing the nodules’ non-silicate components, shows high concentrations of MnO, P2O5, and FeO and a molar ratio P/Mn = 2. Visible to near-infrared reflectance of the nodules (by ChemCam passive and Mastcam multispectral) is dark and relatively flat, consistent with a mixture of host siltstone, hematite, and a dark spectrally bland material (like pyrolusite, MnO2). A drill sample at the site is shown to contain minimal nodule material, implying that analyses by the CheMin and SAM instruments do not constrain the nodules’ mineralogy or composition. The fact that the nodules contain P and Mn in a small molar integer ratio, P/Mn = 2, suggests that the nodules contained a stoichiometric Mn-phosphate mineral, in which Fe did (i.e., could) not substitute for Mn. The most likely such minerals are laueite and strunzite, Mn2+Fe3+2(PO4)2(OH)2·8H2O and –6H2O, respectively, which occur on Earth as alteration products of other Mn-bearing phosphates including vivianite. Vivianite is a common primary and diagenetic precipitate from low-oxygen, P-enriched waters. Calculated phase equilibria show Mn-bearing vivianite could be replaced by laueite or strunzite and then by hematite plus pyrolusite as the system became more oxidizing and acidic. These data suggest that the nodules originated as vivianite, forming as euhedral crystals in the sediment, enclosing sediment grains as they grew. After formation, the nodules were oxidized—first to laueite/strunzite yielding the diagnostic P/Mn ratio, and then to hematite plus an undefined Mn oxy-hydroxide (like pyrolusite). The limited occurrence of these Mn-Fe-P nodules, both in space and time (i.e., stratigraphic position), suggests a local control on their origin. By terrestrial analogies, it is possible that the nodules precipitated near a spring or seep of Mn-rich water, generated during alteration of olivine in the underlying sediments. Full article
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26 pages, 2031 KiB  
Article
Evaluation of the Quality of Raspberries (Rubus idaeus L.) Grown in Balanced Fertilization Conditions
by Barbara Sawicka, Piotr Barbaś, Dominika Skiba, Barbara Krochmal-Marczak and Piotr Pszczółkowski
Commodities 2023, 2(3), 220-245; https://doi.org/10.3390/commodities2030014 - 11 Jul 2023
Cited by 7 | Viewed by 4325
Abstract
(Background) Raspberry (R. idaeus L.) is very popular with consumers around the world for its intense flavor, attractive appearance, and health benefits. In recent years, interest in healthy eating and natural products has increased, and raspberry fits perfectly into these trends, which [...] Read more.
(Background) Raspberry (R. idaeus L.) is very popular with consumers around the world for its intense flavor, attractive appearance, and health benefits. In recent years, interest in healthy eating and natural products has increased, and raspberry fits perfectly into these trends, which translates into its greater importance on the consumer market. (Aim) The aim of this study was the commodity evaluation of raspberry fruits bearing fruit on 2-year-old shoots, cultivated under conditions of varied nitrogen fertilization against the background of constant phosphorus-potassium fertilization. (Methodology) The first-order factors were cultivars (‘Laszka’ and ‘Glen Ample’), and the second-order factor was nitrogen fertilization (0, 50, 100, and 150 kg N ha−1), against the background of constant phosphorus-potassium fertilization (100 kg P2O5 and 120 kg K2O ha−1). The experiment was set up in a dependent split-plot design with three repetitions. (Results) The importance of raspberry on the consumer market was shaped by taste and quality of fruit, health benefits, naturalness and freshness, universality of use, availability, and nutritional trends. (Conclusions) The tested cultivars were characterized by similar production and quality capabilities. Fertilization of the tested cultivars with a dose of 135 kg N·ha−1 turned out to be justified in terms of yield. Increasing nitrogen doses resulted in a significant increase in fresh fruit yield and fruit weight. Different doses of nitrogen increased fruit resistance to mechanical damage, firmness, and quality indices. Full article
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20 pages, 2613 KiB  
Article
Impact of Stratospheric Geoengineering on Sea Surface Temperature in the Northern Gulf of Guinea
by Francis F. B. K. Ayissi, Casimir Y. Da Allada, Ezinvi Baloïtcha, Simone Tilmes and Peter J. Irvine
Climate 2023, 11(4), 87; https://doi.org/10.3390/cli11040087 - 12 Apr 2023
Cited by 5 | Viewed by 3467
Abstract
Among techniques proposed to limit global warming, there is Stratospheric Aerosol Geoengineering (SAG) which is aiming to increase Earth-atmosphere albedo by injecting sulfur dioxide into the stratosphere in order to reduce the solar radiation that reaches the earth. This study aims to assess [...] Read more.
Among techniques proposed to limit global warming, there is Stratospheric Aerosol Geoengineering (SAG) which is aiming to increase Earth-atmosphere albedo by injecting sulfur dioxide into the stratosphere in order to reduce the solar radiation that reaches the earth. This study aims to assess the potential impact of SAG on Sea Surface Temperature (SST) in the Northern Gulf of Guinea and its causes using GLENS (Geoengineering Large Ensemble) simulations performed under a high anthropogenic emission scenario (RCP8.5). Here, we focus on two dynamically different regions: Sassandra Upwelling in Côte d’Ivoire (SUC, located east of Cape Palmas) and Takoradi Upwelling in Ghana (TUG, located east of Cape Three Points). Results show that in the SUC region, under climate change, there is an increase in SST (referred to as the current climate) all year long (by 1.52 °C on average) mainly due to an increase in net heat flux (lead by the decrease in longwave radiation) and also in weak vertical mixing (caused by strong stratification which dominates the vertical shear). Under SAG, SST decreases all the seasonal cycle with its maximum in December (−0.4 °C) due to a reduction in the net heat flux (caused by a diminution of solar radiation) and an increase in vertical advection (due to an increase in vertical temperature gradient and vertical velocity). In the TUG region, under climate change, SST warming is a little more intense than in the SUC region and SST changes are driven by an increase in the net heat flux and strong stratification. The cooling of the SST in TUG is similar to the SUC region, but contrary to this region, the cooling under SAG is not only explained by a decrease in the net heat flux but also by the remote forcing of wind changes at the western equatorial Atlantic. Full article
(This article belongs to the Section Climate Adaptation and Mitigation)
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20 pages, 6043 KiB  
Article
Impacts of Solar Radiation Management on Hydro-Climatic Extremes in Southeast Asia
by Mou Leong Tan, Liew Juneng, Heri Kuswanto, Hong Xuan Do and Fei Zhang
Water 2023, 15(6), 1089; https://doi.org/10.3390/w15061089 - 13 Mar 2023
Cited by 18 | Viewed by 6924
Abstract
Solar radiation management (SRM), or solar geoengineering, reduces the earth’s temperature by reflecting more sunlight back to space. However, the impacts of SRM remain unclear, making it difficult to project the benefits as well as consequences should this approach be adopted to combat [...] Read more.
Solar radiation management (SRM), or solar geoengineering, reduces the earth’s temperature by reflecting more sunlight back to space. However, the impacts of SRM remain unclear, making it difficult to project the benefits as well as consequences should this approach be adopted to combat climate change. To provide novel insight into the SRM impact on hydro-climatic extremes in Southeast Asia, this study conducts a simulation experiment for the Kelantan River Basin (KRB) in Malaysia by incorporating three bias-corrected Stratospheric Aerosol Geoengineering Large Ensemble (GLENS) members into the Soil and Water Assessment Tool Plus (SWAT+) model. The study found that SRM practices could generate substantial cooling effects on regional temperatures, leading to a reduction in projected annual precipitation and monthly precipitation during the flooding season (from November to mid-January) under SRM relative to the Representative Concentration Pathway 8.5 (RCP8.5) scenario. In addition, SRM could reduce the number of days with heavy precipitation as well as the intensity of maximum daily precipitation as compared to RCP8.5, during the 2045–2064 and 2065–2084 periods, leading to a reduction in high flows. Nevertheless, under SRM impacts, the driest months from February to May would experience comparable decreases in monthly precipitation and streamflow. Full article
(This article belongs to the Section Water and Climate Change)
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27 pages, 8023 KiB  
Article
The Main Morphological Characteristics and Chemical Components of Fruits and the Possibilities of Their Improvement in Raspberry Breeding
by Irina Titirică, Ioana A. Roman, Claudia Nicola, Monica Sturzeanu, Elena Iurea, Mihai Botu, Radu E. Sestras, Rodica Pop, Mădălina Militaru, Sezai Ercisli and Adriana F. Sestras
Horticulturae 2023, 9(1), 50; https://doi.org/10.3390/horticulturae9010050 - 3 Jan 2023
Cited by 16 | Viewed by 3897
Abstract
Raspberry is a delicious fruit with important nutritional and health properties for consumers. The efficiency of achieving raspberry breeding aims such as productivity, fruit quality, and adequate response to stressors, etc., depends directly on knowledge of the inheritance of traits and genetic factors [...] Read more.
Raspberry is a delicious fruit with important nutritional and health properties for consumers. The efficiency of achieving raspberry breeding aims such as productivity, fruit quality, and adequate response to stressors, etc., depends directly on knowledge of the inheritance of traits and genetic factors of influence and their pragmatic use. In this study, the main morphological characteristics and chemical components of fruits of 24 raspberry genotypes were studied in a comparative field trial; 14 were promising selections and the other 10 included their parental forms (Autumn Bliss, Glen Moy, Glen Prosen, Opal, Pathfinder, Titan, Tulameen, Veten, Willamette) and Glen Ample cultivar, which was used as control. The results highlighted significant differences and a large variation among the genotypes (between 2.40 and 4.90 g for fruit weight, 0.19–0.47 kg/cane for yield, 50–106 for drupelets/fruit, 10.0–12.7% for dry matter, 0.23–2.00% pectin, 1.61–2.72% glucose, etc.). The inheritance of the analyzed traits, considered quantitative, was different, but a low heritability was recorded for non-marketable fruits and the number of drupelets in fruit. In several hybrid selections, negative heterosis prevailed, highlighting the breeding difficulties of the important desired traits of the species. The values of heterosis and the broad-sense and narrow-sense heritability of some traits demonstrate that, through judiciously choosing parents, raspberry breeding in the desired direction can be successful. Full article
(This article belongs to the Special Issue New Results in Fruit Tree Breeding and Efficient Use of Cultivars)
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14 pages, 852 KiB  
Communication
Global Methylation Analysis Using MSAP Reveals Differences in Chilling-Associated DNA Methylation Changes during Dormancy Release in Contrasting Sweet Cherry Varieties
by Gabriela Narváez, Claudia Muñoz-Espinoza, Esteban Soto, Karin Rothkegel, Macarena Bastías, José Gutiérrez, Soraya Bravo, Rodrigo Hasbún, Claudio Meneses and Andrea Miyasaka Almeida
Horticulturae 2022, 8(10), 962; https://doi.org/10.3390/horticulturae8100962 - 18 Oct 2022
Cited by 2 | Viewed by 3265
Abstract
Dormancy is an adaptive strategy developed by temperate perennial crops to protect overwinter tissues from unfavorable environmental conditions. Sweet cherry (Prunus avium L.), a member of the Rosaceae family, requires chilling to overcome dormancy. The time of harvest is directly correlated with [...] Read more.
Dormancy is an adaptive strategy developed by temperate perennial crops to protect overwinter tissues from unfavorable environmental conditions. Sweet cherry (Prunus avium L.), a member of the Rosaceae family, requires chilling to overcome dormancy. The time of harvest is directly correlated with chilling requirements in sweet cherries. Consequently, early and late season varieties have low and high chilling requirements, respectively. There is evidence that the expression of dormancy-related genes is regulated by DNA methylation. In this work, methylation-sensitive amplified polymorphism (MSAP) was applied to study genome-wide DNA methylation changes associated with dormancy in two low-chill varieties, ‘Royal Dawn’ and ‘Glen Red’, and one high-chill variety, ‘Kordia’. Our primary results suggest that the occurrence of progressive DNA demethylation is associated with chilling accumulation during dormancy in the three varieties, independent of their chilling requirements. Genes were identified with different methylation status changes, detected by MSAP, related to cell wall remodeling and energy metabolism. Several MSAP profiles among the varieties were observed, suggesting that fine epigenetic control is required to coordinate hormonal and environmental signals that induce dormancy and its release. Full article
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10 pages, 1248 KiB  
Article
Sulfur Nutrition Affects Garlic Bulb Yield and Allicin Concentration
by Binh Thi Nguyen, Stephen M. Harper, Tim J. O’Hare, Neal W. Menzies and Bernhard Wehr
Plants 2022, 11(19), 2571; https://doi.org/10.3390/plants11192571 - 29 Sep 2022
Cited by 5 | Viewed by 3590
Abstract
Improving bulb yield and allicin content of garlic is important in meeting fresh and pharmaceutical market demands. Garlic plants have a high demand for sulfur (S) since allicin contains S atoms. Two experiments were conducted to identify the effect of S application rate [...] Read more.
Improving bulb yield and allicin content of garlic is important in meeting fresh and pharmaceutical market demands. Garlic plants have a high demand for sulfur (S) since allicin contains S atoms. Two experiments were conducted to identify the effect of S application rate on garlic yield and quality. In a field trial assessing six S application rates (0–150 kg S ha−1), cultivar ‘Glenlarge’ produced the greatest bulb weight (~90 g) and allicin content (521 mg bulb−1) with the application of 75 kg S ha−1. In contrast, cultivar ‘Southern Glen’ showed no response in bulb weight or allicin. This was likely due to high soil background S concentrations masking treatment effects. Subsequently, a solution culture experiment with cv. ‘Glenlarge’ evaluated six S application rates (188 to 1504 mg S plant−1, nominally equivalent to 25–200 kg S ha−1). In solution culture, bulb weight and allicin concentration increased with S rate. Highest bulb weight (~53 g bulb−1) and allicin concentration (~11 mg g−1 DW) were recorded at an S application of 1504 mg S plant−1. This is the first report to conclusively demonstrate the effect of S on yield and allicin in garlic grown in solution culture. Full article
(This article belongs to the Special Issue Plant Nutrition Volume II)
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27 pages, 3927 KiB  
Article
Nectar Abundance and Nectar Composition in Selected Rubus idaeus L. Varieties
by Mikołaj Kostryco and Mirosława Chwil
Agriculture 2022, 12(8), 1132; https://doi.org/10.3390/agriculture12081132 - 30 Jul 2022
Cited by 8 | Viewed by 3796
Abstract
The increasing commercial production of R. idaeus offers insects nectar and pollen rewards, thus increasing the chance of cross-pollination, which enhances fruit yields. The knowledge of nectar characteristics may help farmers/beekeepers to improve the quality of their products. Therefore, we determined and compared [...] Read more.
The increasing commercial production of R. idaeus offers insects nectar and pollen rewards, thus increasing the chance of cross-pollination, which enhances fruit yields. The knowledge of nectar characteristics may help farmers/beekeepers to improve the quality of their products. Therefore, we determined and compared nectar weight, sugar concentration and weight, and the qualitative and quantitative composition of sugars and amino acids in the nectar of six raspberry cultivars: three biennial and three repeated fruiting cultivars. The nectary abundance in these cultivars ranged between 20.2 ± 3.84 mg (‘Polka’) and 26.4 ± 7.3 mg (‘Glen Ample’) of nectar per flower with a sugar concentration of 34.6 ± 5.61–47.3 ± 9.33%. The contents of glucose and fructose (g/100 g) were in the range from 42.96 ± 0.71 (‘Glen Ample’) to 46.94 ± 0.55 (‘Laszka’) and from 50.7 ± 1.43 (‘Polka’) to 54.2 ± 0.72 (‘Radziejowa’). Sucrose was detected only in ‘Glen Ample’ (5.6 ± 1.12 g/100 g) and ‘Polka’ (6.2 ± 0.95 g/100 g). Taking into account the increasing sugar weight in the nectar, the cultivars were ranked as follows: ‘Polka’ < ‘Polana’ < ‘Radziejowa’ < ‘Pokusa’ < ‘Laszka’ < ‘Glen Ample’. The nectar of the analyzed raspberry cultivars was classified as a hexose-dominant type. Aspartic acid, glutamic acid, and proline were the most dominant endogenous amino acids, whereas exogenous acids were dominated by lysine and leucine. The present results provide valuable information about the nutritious value of R. idaeus nectar for pollinators. Full article
(This article belongs to the Section Farm Animal Production)
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37 pages, 13737 KiB  
Article
Nectar Secretion, Morphology, Anatomy and Ultrastructure of Floral Nectary in Selected Rubus idaeus L. Varieties
by Mikołaj Kostryco and Mirosława Chwil
Agriculture 2022, 12(7), 1017; https://doi.org/10.3390/agriculture12071017 - 13 Jul 2022
Cited by 6 | Viewed by 3876
Abstract
The distinctive features of floral nectaries facilitate identification of ecological and phylogenetic links between related taxa. The structure and functioning of nectaries determine the relationships between plants, pollinators, and the environment. The aim of the study was to determine and compare the micromorphology [...] Read more.
The distinctive features of floral nectaries facilitate identification of ecological and phylogenetic links between related taxa. The structure and functioning of nectaries determine the relationships between plants, pollinators, and the environment. The aim of the study was to determine and compare the micromorphology of the epidermis in the floral nectaries of six Rubus idaeus cultivars belonging to biennial (‘Glen Ample’, ‘Laszka’, ‘Radziejowa’) and repeated fruiting (‘Pokusa’, ‘Polana’, ‘Polka’) groups. Another objective was to characterize the cuticle ornamentation and stomatal morphology, the anatomy of the nectary epidermis, parenchyma, and sub-nectary parenchyma in the initial nectar secretion phase, as well as the ultrastructure of the nectary epidermis and parenchyma cells in the initial and full nectar secretion phases. The study was carried out using light, fluorescence, scanning and transmission-electron microscopy techniques. Semi-thin and ultrathin sections were used for the microscopic analyses. The cuticular ornamentation and stomatal morphology may be helpful elements in the identification of relatedness between Rubus species. The interaction of the extensive system of endoplasmic reticulum membranes, mitochondria, and Golgi apparatus indicates high metabolic activity, and the fusion of transport vesicles with the membrane suggests granulocrine nectar secretion. The results bring new data to the biology of plants. Full article
(This article belongs to the Section Farm Animal Production)
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