Search Results (71)

Indonesia’s worsening air pollution and traffic emissions have thrust electric vehicles (EVs) into the spotlight, but what really drives Indonesians to make the switch? This study integrates Protection Motivation Theory with green branding and policy frameworks to explain electric vehicle (EV) adoption in Indonesia. Using a nationwide survey (n = 986) and partial-least-squares structural-equation modeling, we test how environmental awareness, consumer expectancy, threat appraisal, and coping appraisal shape adoption both directly and through green brand image (GBI), while perceived policy incentives moderate the GBI–adoption link. The model accounts for 54% of the variance in adoption intention. These findings highlight that combining public awareness campaigns, compelling green brand messaging, and carefully calibrated policy incentives is essential for accelerating Indonesia’s transition to cleaner transport. Full article

Objectives: To evaluate and compare the plaque-reducing efficacy of sesame-based oil pulling versus distilled water in a randomized controlled, examiner-blinded parallel-group study. Materials and Methods: Forty participants with gingivitis (community periodontal index of treatment needs grade 1 or 2) were randomly assigned to either the test group (sesame-based oil) or the control group (distilled water). Participants were instructed to perform oil pulling daily in the morning for 15 min over an eight-week period. The Rustogi Modified Navy Plaque Index (RMNPI) and gingival bleeding index (GBI) were evaluated at the baseline, as well as after four and eight weeks. Additionally, biofilm samples were collected for microbiological analysis. Results: The RMNPI was statistically significantly reduced after eight weeks of pulling with sesame-based oil (p < 0.001), as well as with distilled water (p < 0.001), without a significant difference between the groups. The GBI was statistically significantly reduced after eight weeks of pulling with sesame-based oil (p < 0.002), as well as with distilled water (p < 0.002), without a significant difference between the groups. No significant microbiological changes were detected in biofilm samples. Conclusions: Both plaque and gingival indices significantly decreased with oil pulling after eight weeks of intervention. Preclinical studies are necessary to clarify the mechanism of plaque reduction by oil pulling. Full article

Under the background of climate change, the risk spillover within the energy system is constantly intensifying. Clarifying the coupling relationship between entities within the energy system can help policymakers propose more reasonable policy measures and strengthen risk prevention. To estimate the risk spillover of energy-related systems, this paper constructs five subsystems: the fossil fuel subsystem, the electricity subsystem, the green bond subsystem, the renewable energy subsystem, and the carbon subsystem. Then, a quantitative risk analysis is conducted on two major energy consumption/carbon emission entities, China and Europe, based on the DCC-GARCH-CoVaR method. The result shows that (1) Markets of the same type often have more significant dynamic correlations. Of these, the average dynamic correlation coefficient of GBI-CABI (the Chinese green bond subsystem) and FR-DE (the European electricity subsystem) are the largest, by 0.8552 and 0.7347. (2) The high correlation between energy markets results in serious risk contagion, and the overall risk spillover effect within the European energy system is about 2.6 times that within the Chinese energy system. Of these, EUA and CABI are the main risk connectors of each energy system. Full article

This study investigates the mechanisms through which employees’ green behavioural intention (GBI) translates into employee in-role green behaviour (EGB-IR) in the hospitality sector. Drawing on the Theory of Planned Behaviour (TPB) and the Value-Belief Norm (VBN) theory, we propose an extended model that incorporates four contextual mediators —customers’ environmental attitudes (CEAs), coworkers’ green work climate (CGWC), supervisors’ commitment to the environment (SCE), and environmental organisational policy (EOP)—to explain how the relationship between intentions and behaviours is mediated by these social and organisational factors. Data were collected through a self-administered survey of 497 employees from hotels located in the Canary Islands, a recognised coastal tourism destination facing relevant environmental challenges. The proposed relationships were tested using Partial Least Squares Structural Equation Modelling (PLS-SEM). Results confirm that GBI significantly predicts in-role green behaviour (EGB-IR), and all four contextual factors partially mediate this relationship. Among them, EOP and CGWC emerged as the most influential mediators. These findings underscore the importance of aligning individual motivation with supportive workplace environments to foster consistent sustainable practices in hospitality organisations. This research contributes to the growing literature on green behaviours in hospitality by empirically validating a multilevel TPB-VBN-theory-based framework and highlighting key points for hotel managers aiming to strengthen their environmental commitment through employee engagement. Full article

Functional foods are evolving beyond basic nutrition to address nutrition-related diseases and enhance well-being. While probiotic-fortified products dominate this sector, most remain dairy-based. This study investigated the incorporation of Lactobacillus rhamnosus GG and Bacillus coagulans GBI-30 into cereal-based pasta and noodles, evaluating bacterial survival during processing and cooking. Extrusion-based pasta production exerted greater stress on Lactobacillus rhamnosus GG, whereas Bacillus coagulans GBI-30 demonstrated higher thermal resistance. In sheeted noodles, both strains maintained ≥8 log CFU/g viability pre-cooking. After 7 min boiling, Lactobacillus rhamnosus GG retained 6.88 log CFU/g and Bacillus coagulans GBI-30 5.75 log CFU/g in noodles, meeting the recommended 10⁶–10⁷ CFU/g threshold for probiotic efficacy. Cooking performance analysis revealed lower cooking loss in noodles (2.4–4.04%) versus extruded pasta (10.6–19.05%), indicating superior structural integrity. These results confirm cereal matrices as viable non-dairy carriers for probiotics, with sheeting processes better preserving bacterial viability than extrusion. The findings highlight a practical strategy for developing functional foods that sustain probiotic viability through processing and consumption, potentially enhancing gut microbiota balance. This approach expands probiotic delivery options beyond traditional dairy formats while maintaining therapeutic bacterial concentrations critical for health benefits. Full article

The benzimidazole core (BI) plays a central role in biologically active molecules. The BI nucleus is widely used as a building block to generate a variety of bioactive heterocyclic compounds to be used as antihelmintics, antiprotozoal, antimalarials, anti-inflammatories, antivirals, antimicrobials, antiparasitics, and antimycobacterials. A versatile BI derivative is the 2-guanidinobenzimidazole (2GBI), which, together with its derivatives, is a very interesting poly-functional planar molecule having a delocalised 10 π electrons system conjugated with the guanidine group. The 2GBI molecule has five nitrogen atoms containing five labile N–H bonds, which interact with the out-ward-facing channel entrance, forming a labile complex with the biological receptor sites. In this work, 2GBI and their derivatives were analyzed as ligands to form host–guest, coordination and organometallic complexes. Synthesis methodology, metal geometries, hydrogen bonding (HB) interactions, and the biological activities of the complexes were discussed. Full article

In recent years, increased environmental awareness has led consumers to adopt more eco-friendly lifestyles, including choosing green products to reduce their impact on the environment. With a particular focus on the green consumption behavior with regard to battery electric vehicles (BEVs) from two leading brands, Tesla (automotive) and Gogoro (scooter), in Taiwan, this study investigated the relationships among green perceived quality (GPQ), green perceived value (GPV), green brand image (GBI), green trust (GTR), and green purchase intention (GPI). After collecting 203 environmentally conscious respondents who are familiar with Tesla and Gogoro BEVs, the PLS-SEM was used to verify the research framework. The findings demonstrate that GPQ significantly impacts GBI and GPV; GBI significantly impacts GPV and GTR; and GPV significantly impacts GTR. As the key determinants of purchasing BEVs, GPQ, GBI, and GTR have significant effects on GPI, whereas the effect of GPV on GPI is not significant. In addition, this study also explored the moderation effect on green purchase behavior, revealing that the brand-specific cognition of Tesla and Gogoro moderates the relationships between green attributes (such as GPQ, GBI, and GTR) and GPI. This study contributes a more comprehensive understanding of the psychological and behavioral drivers behind green consumption behavior within the context of BEVs. This study not only sheds light on consumer behavior within dual-market dynamics in Taiwan but also offers a framework for other markets with similar environmental and infrastructural challenges. These insights can assist BEV companies to develop their sustainable marketing strategies, emphasizing the importance of developing a strong green brand image and green trust to boost BEV sales from the perspective of green perceived quality. Full article

Computational thinking (CT) in young children (K to three) has been of much interest among educational researchers due to the applicability of CT to solving problems in daily life and various academic disciplines. This study uses existing data from children’s gameplay in a block-based programming game called codeSpark Academy to examine the extent to which we can use children’s gameplay behavior to measure their CT and, more generally, their problem-solving skills. The objectives of the study are to operationalize CT and problem-solving constructs using gameplay data, investigate the relationship between CT and problem-solving, and position codeSpark Academy as a valid assessment tool. A total of 72 elementary students (aged 6–9) played codeSpark Academy once a week for six weeks. TechCheck, an externally developed and validated measure of CT, was administered before the first game day and after the last game day. Using fine-grained, moment-to-moment gameplay data, we developed and validated seven game-based indicators (GBIs) of CT using correlational analysis and nonparametric tests and integrated them into a problem-solving framework. Our findings showed that children’s gameplay behavior can be used to measure their CT and problem-solving skills. Full article

Objective: Liver fibrosis, a hallmark of chronic liver diseases, is characterized by excessive extracellular matrix (ECM) deposition and scar tissue formation. Current antifibrotic nanomedicines face significant limitations, including poor penetration into fibrotic tissue, rapid clearance, and suboptimal therapeutic efficacy. The dense fibrotic ECM acts as a major physiological barrier, necessitating the development of a targeted delivery strategy to achieve effective therapeutic outcomes. Methods: We designed a liposomal delivery system functionalized with the GBI-10 aptamer and encapsulating obeticholic acid (OCA lips@Apt) to enhance selective delivery to fibrotic liver tissue while minimizing systemic toxicity. Results: Both in vitro and in vivo studies demonstrated that the aptamer-modified OCA liposomes effectively treated hepatic fibrosis through dual mechanisms: modulation of abnormal bile acid metabolism and attenuation of inflammation. The targeted delivery system leveraged the overexpression of Tenascin-C (TnC), a key ECM component in fibrotic tissues, for precise localization and enhanced endocytosis via the exposed cationic liposome surface. Conclusions: The OCA lips@Apt nanodrug demonstrated superior therapeutic efficacy with minimal off-target effects, offering a promising strategy to overcome critical barriers in liver fibrosis treatment. By precisely targeting the fibrotic ECM and modulating key pathological pathways, this TnC-guided liposomal delivery system provides a significant advancement in antifibrotic nanomedicine. Full article

Greenbug, Schizaphis graminum, is one of the important cereal aphid pests of sorghum in the United States and other parts of the world. Sorghum bicolor variety PI 607900 carries the Schizaphis graminum resistance (SgR1) gene that underlies plant resistance to greenbug biotype I (GBI). Now, the SgR1 has been determined as the major gene conferring greenbug resistance based on the strong association of its presence with the resistance phenotype in sorghum. In this study, we have successfully isolated the SgR1 gene using a map-based cloning approach, and subsequent molecular characterization revealed it encodes a leucine-rich repeat containing receptor-like protein (LRR-RLP). According to DNA sequence analysis, the SgR1 gene are conserved among GBI-resistance sorghum accessions but are variable within susceptible lines. Furthermore, an InDel (−965 nt) at its promoter region and a single-nucleotide polymorphism (SNP, 592 nt) in the CDS of the SgR1 were detected and they are well conserved within resistant genotypes. When the SgR1 gene was cloned and transferred into Arabidopsis plants, the SgR1 was activated in the transgenic Arabidopsis plants in response to attack by green peach aphids according to the results of the histochemical assay, and GUS activity was detected in situ in spots around the vasculature of the leaf where the phloem is located, suggesting its biological function in those transgenic Arabidopsis plants. Overall, this study confirms that the SgR1 gene coding for an LRR-RLP is the major resistance gene to greenbug, a destructive pest in sorghum and wheat. This represents the first greenbug resistance gene cloned so far and indicates that the simple-inherited GBI resistance gene can be used for sorghum improvement with genetic resistance to GBI via molecular breeding or cross-based conventional breeding technologies. Full article

Background/Objectives: Disruptions in lacrimal function can lead to various conditions, including chronic nasolacrimal duct obstruction and dacryocystitis, which may cause symptoms like excessive tearing, pain, and recurrent infections. In cases where pharmacological treatments are insufficient, surgical intervention becomes essential, which is widely used to restore effective tear drainage. Methods: This study evaluated postoperative outcomes of laser-assisted dacryocystorhinostomy (LDCR) in 48 patients, totaling 56 treated eyes, over a follow-up period of 6 months to 3.5 years. During the follow-up examination, patients completed the Glasgow Benefit Inventory questionnaire. Results: The outcomes demonstrated significant improvements, particularly in the GBI total score (+35.07) and general subscale (+44.36), with minimal effects on social support and physical health subscales. Conclusions: LDCR proves to be an effective, minimally invasive alternative to traditional dacryocystorhinostomy, offering significant patient satisfaction, faster recovery, and reduced complications in treating nasolacrimal duct obstruction. Full article

The transition towards more sustainable packaging calls for improving our ability to predict, control, and inhibit microbial growth. Despite the importance of modified atmosphere packaging (MAP) in food preservation, the exact relations between MAP gases (CO₂, O₂, N₂) and microbial behavior are still poorly understood. Addressing this major knowledge gap requires a specific infrastructure to gain precise control over the gas composition during storage time. Thus, this study aimed at developing and validating an innovative gas-washing bottle incubation system (GBIS) with an adapted pH methodology for monitoring microbial growth in liquid media under different well-controlled conditions. Listeria monocytogenes—a psychrotrophic pathogen raising severe safety concerns under refrigerated conditions—was used as a representative microorganism. The results showed that daily gas flushing effectively dominated overnight headspace variations, allowing incubating L. monocytogenes and daily sampling for 13 days under static conditions. Subsequently, storage experiments were performed at 4 °C under stable pH and anaerobic conditions with different CO₂ levels (20–40–60%). Significant growth reduction was observed from 6.0 to 4.8 log CFU/mL as CO₂ increased from 20% (pH = 6.7) to 60% (pH = 6.2) (p ≤ 0.05). Overall, GBIS shows great potential in data collection for predictive modeling and, consecutively, for boosting decision-making in the food packaging sector. Full article

Arthrospira platensis (Ap) and Bacillus coagulans (Bc) have been successfully used to develop functional foods, but a combination of both regarding functional implications in nutritional value and antioxidant capacity has not been explored. This work aimed to develop an artisanal wheat flour pasta with egg using 5% A. platensis and 1% B. coagulans GBI 6068 (labeled as Bc+Ap). Uncooked pasta was characterized regarding nutritional value; furthermore, total phenolic content, antioxidant capacity by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and ferric reducing antioxidant power (FRAP), pigment content, colorimetry assay, textural profile analysis, buffering capacity, and probiotic viability were carried out on uncooked and cooked pasta to assess the changes induced by cooking. The Bc+Ap pasta showed enhanced nutritional value with a significant increase in protein content (30.61%). After cooking, the pasta showed increased phenolic content (14.22% mg GAE/g) and antioxidant capacity (55.59% µmol Trolox equivalents/g and 10.88% µmol Fe⁺²/g) for ABTS and FRAP, respectively, as well as pigment content (6.72 and 1.17 mg/100 g) for chlorophyll a+b and total carotenoids, respectively, but relative impacts on colorimetric parameters in contrast to control (wheat flour pasta). Furthermore, Bc+Ap showed improved firmness (59%, measured in g), buffer capacity (87.80% μmol H⁺(g × ΔpH)⁻¹), and good probiotic viability (7.2 ± 0.17 log CFU/g) after the cooking process. Full article

The influence of street spatial form on thermal comfort from urban morphology and human-centered perspectives has been underexplored. This study, utilizing multi-source data and focusing on urban central districts, establishes a refined index system for street spatial form and a thermal comfort prediction model based on extreme gradient boosting (XGBoost) and Shapley additive explanations (SHAP). The results reveal the following: (1) Thermal comfort levels display spatial heterogeneity, with areas of thermal discomfort concentrated in commercial zones and plaza spaces. (2) Compared to the human-centered perspective, urban morphology indicators correlate strongly with thermal comfort. (3) The key factors influencing thermal comfort, in descending order of importance, are distance from green and blue infrastructure (GBI), tree visibility factor (TVF), street aspect ratio (H/W), orientation, functional diversity indices, and sky view factor. All but the TVF negatively correlates with thermal comfort. (4) In local analyses, the primary factors affecting thermal comfort vary across streets with different heat-risk levels. In high heat-risk streets, thermal comfort is mainly influenced by distance from GBI, H/W, and orientation, whereas in low heat-risk streets, vegetation-related factors dominate. These findings provide a new methodological approach for optimizing urban thermal environments from both urban and human perspectives, offering theoretical insights for creating more comfortable cities. Full article

Background: α-dystroglycanopathies are congenital muscular dystrophies in which genetic mutations cause the decrease or absence of a unique and complex O-linked glycan called matriglycan. This hypoglycosylation of O-linked matriglycan on the α-dystroglycan (α-DG) protein subunit abolishes or reduces the protein binding to extracellular ligands such as laminins in skeletal muscles, leading to compromised survival of muscle cells after contraction. Methods: Surrogate molecular linkers reconnecting laminin-211 and the dystroglycan β-subunit through bispecific antibodies can be engineered to improve muscle function in the α-dystroglycanopathies. This study reports the metabolic engineering of a novel glycofusion bispecific (GBi) antibody that fuses the mucin-like domain of the α-DG to the light chain of an anti-β-DG subunit antibody. Results: Transient HEK production with the co-transfection of LARGE1, the glycoenzyme responsible for the matriglycan modification, produced the GBi antibody only with a light matriglycan modification and a weak laminin-211 binding activity. However, when a sugar feed mixture of uridine, galactose, and manganese ion (Mn²⁺) was added to the culture medium, the GBi antibody produced exhibited a dramatically enhanced matriglycan modification and a much stronger laminin-binding activity. Conclusions: Further investigation has revealed that Mn²⁺ in the sugar feeds played a critical role in increasing the matriglycan modification of the GBi antibody, key for the function of the resulting bispecific antibody. Full article