Search Results (57)

This study aimed to reveal intraspecific variations in two Dryomys species distributed in Türkiye, based on mitochondrial DNA cytochrome b gene sequences, and to discuss the factors driving these variations in the context of phylogeography and genetic species concepts. As a result of Maximum Likelihood, Bayesian Inference, and Network analyses, which included haplogroups or lineages from Italy, Russia, the Caucasus, and Iran identified in previous studies, along with Turkish haplotypes, three major clades (MC1, MC2, and MC3) were identified within Dryomys nitedula. These clades began to diverge evolutionarily in the middle of the Late Miocene (8.82 million years ago) and exhibit significant genetic differences from one another. The Turkish haplotypes were divided into five distinct lineages (N1–N5), each within five subclades (SC1–SC5), which were nested within these MCs. These lineages, their geographical distributions, and the subspecies defined in previous studies that correspond to these lineages are as follows: N1 from the Thrace region (Dryomys nitedula wingei), N2 from the Black Sea region (potentially a new subspecies), N3 from western and central Anatolia (Dryomys nitedula phrygius), N4 from northeastern Anatolia (Dryomys nitedula tichomirowi), and N5 from eastern Anatolia (Dryomys nitedula pictus). The N2 lineage, distributed in areas close to the coastal side of the Eastern Black Sea region and with a range close to both N3 (D. n. phrygius) and N4 (D. n. tichomirowi), exhibited high genetic differentiation from these two lineages and was a candidate to be treated as a new subspecies of Dryomys nitedula in Türkiye. The N5 lineage, which includes haplotypes from the distribution areas of the populations initially classified as Dryomys pictus and later as Dryomys nitedula pictus in previous studies, was found to be more closely related to Dryomys nitedula kurdistanicus from the Zagros Mountains than to D. n. pictus from the central regions of Iran. Combining the results of this study with previous research, it is clear that the D. nitedula lineages in Türkiye, along with haplogroups or subspecies in neighboring regions diverged between the middle Late Miocene and Middle Pleistocene. This divergence is believed to have been driven by climatic cycles and geomorphological processes that shaped the topography of their distribution range. The high genetic diversity observed in the lineages of Anatolia suggests that the region may have served as a glacial refuge for D. nitedula. Similarly to the processes and factors shaping the evolution of D. nitedula, Dryomys laniger was found to have diverged into two lineages, western (L1) and eastern (L2 or Dryomys anatolicus), within its distribution range during the Late Pliocene (2.94 Mya). To make a more accurate taxonomic assessment of D. laniger, a larger number of samples is needed, and the distribution limits should be more clearly defined. Full article

Sediment transportation on coasts can be significantly affected by rivers, wave–wind effects, and human activities. As a result, undesirable effects such as shoaling or erosion may occur in fishery shelters. This study examines the “Sandıktaş a Fishery Shelter”, a coastal structure in the Eastern Black Sea region of Turkey, and its susceptibility to shoaling. Bathymetric measurements were performed within the nearshore and onshore, and sediment samples were taken periodically from selected points and analyzed in the laboratory. The characteristic grain diameters of the sedimentation were obtained. It was revealed that the average grain diameter was d₅₀ = 0.30–0.91, caused by an increase of 11,611 m³ in shoaling, which caused the decrease of 8 cm water depth that occurred between 2019 and 2022. The entrance of the fishery shelter has become progressively shallower, making it difficult for boats to navigate. Existing breakwater configurations played a role in trapping sediments, requiring optimized breakwater designs/modifications for improved sediment control. The Mann–Kendall test showed an increasing trend in sediment accumulation, particularly in coarser fractions. The findings highlight the necessity of periodic dredging and potential structural modifications to mitigate shoaling and ensure the long-term sustainability of the fishery shelter. Moreover, they emphasize the critical challenges caused by sedimentation in fishery shelters and provide data-driven recommendations for enhancing coastal engineering practices and maintenance strategies. Full article

The Mediterranean fruit fly (medfly), Ceratitis capitata (Wiedemann 1824; Diptera, Tephritidae), is considered one of the most important pests, infesting more than 300 species of fresh fruit and vegetables worldwide. The medfly is an important invasive species, which has spread from the eastern part of sub-Saharan Africa to all of the world’s continents in recent centuries. Currently, the medfly is expanding its geographical range to cooler, temperate areas of the world, including northern areas of Mediterranean countries and continental areas of Central Europe. We collected and analysed all the available information, including in historical records, on the phenology of the medfly in the Balkan Peninsula, to map and understand the path of invasion and spread dynamics on the northern Mediterranean coast and in Central Europe. The medfly was first recorded in the Balkan Peninsula in 1915, in the Aegean area on the island of Aigina, followed by a few records on its presence in the Peloponnese in the early 1930s and throughout the Adriatic coastal area in the 1950s; it was first detected on the Croatian coast in 1947. By 2010, the medfly had been detected along the entire Ionian coast, while the first record of its presence on the Balkan coast of the Black Sea was made in 2005. Since 2000 to date, there has been a significant increase in the frequency of medfly detections in the interior of the Balkan Peninsula, including occasional detections in areas with unfavourable climatic conditions for overwintering, which seems to be favourable for reproduction during the summer and lead to significant infestation of late ripening fruits (late summer and autumn). In the last 20 years, the medfly has spread to more northerly areas (43 to 45 degrees latitude) and has been detected at higher altitudes (>200 to 600 m). Along the Balkan Peninsula, the infestation of fruits from 25 host plant species, from 14 genera and 10 plant families, has been reported. Considering the extremely high invasiveness of the medfly and its wide distribution in several Balkan regions with different climatic conditions, we can assume that it is adapting to new climatic conditions and infesting new host plants. Full article

The Black Sea, a unique semi-enclosed marine ecosystem, is the eastern maritime boundary of the European Union and holds significant ecological importance. The present study investigates anthropogenic noise pollution in the context of the Marine Strategy Framework Directive’s Descriptor 11, with a particular emphasis on the criteria for impulsive sound (D11C1) and continuous low-frequency sound (D11C2) in Romanian ports, which handle a substantial share of regional cargo traffic, and impact maritime activities and associated noise levels. The noise levels from shipping activity vary across Romanian waters, including territorial waters, the contiguous zone, and the Exclusive Economic Zone. These areas are classified by high, medium, and low ship traffic density. Ambient noise levels at frequencies of 63 Hz and 125 Hz, dominated by shipping noise, were established, along with their hydrospatial distribution for the 2019–2020 period. Furthermore, predictive modeling techniques are used in this study to assess underwater noise pollution from human sources. This modeling effort represents the first initiative in the region and utilizes the BELLHOP ray-tracing method for underwater acoustic channel modeling in shallow-water environments. The model incorporates realistic bathymetry, oceanography, and geology features for environmental input, allowing for improved prediction of acoustic variability due to time-varying sea variations in shallow waters. The study’s findings have important implications for understanding and mitigating anthropogenic noise pollution’s impact on the Black Sea marine ecosystem. Full article

In mid-March 2022, a Siberian High brought intense cold air masses, leading to severe weather conditions across southern Europe, including the Black Sea region. This study investigates the spatial and temporal evolution of cold intermediate water (CIW) masses in the Black Sea, with a particular focus on the successive anomalously cold episodes that occurred in March 2022. The research underscores the significance of the northwestern continental slope and cyclonic gyres, especially as the only cold-water mass observations during the warm winters of 2020 and 2021 were concentrated in these areas. Following two warm winters, the cold episodes of March 2022 revealed notable convection and simultaneous cooling, particularly in the cyclonic interior and the Rim Current periphery, excluding the northeastern periphery. Subsequently, cold waters spreading isopycnally throughout the summer months were transported laterally and reached these regions. Argo float measurements provided clear evidence of widespread replenishment of the CIW, indicating that it is not confined to specific areas. The study also highlights regional variability in the characteristics of CIW formation, which is influenced by local dynamics and preconditioning temperatures. The temperatures of CIW increased from west to east, in line with the sea surface temperature gradient. Notably, thicker and colder CIW was found in the western cyclonic gyre compared to the eastern cyclonic area. Furthermore, the study confirms that the warming trend in CIW, identified in previous research, not only continues but has intensified during the recent period analyzed. These findings, observed under the extreme conditions analyzed in this research, offer valuable insights into the widespread occurrence of CIW formation in the Black Sea. Additionally, the study confirms that the warming trend in CIW, identified in previous studies, continued in the region throughout the warm winter period and after the cold spell in 2022. These insights contribute to a deeper understanding of CIW dynamics and their response to extreme weather events in the Black Sea. Full article

The summer of 2024 witnessed record-high sea surface temperatures (SST) across the Aegean, Ionian, and Cretan Seas (AICS), following unprecedented air heatwaves over the sea under a long-term warming trend of 0.46 °C/decade for the mean atmospheric temperature (1982–2024). The respective mean SST trend for the same period is even steeper, increasing by 0.59 °C/decade. With mean summer surface waters surpassing 28 °C, particularly in the Ionian Sea, the southern Cretan, and northern Aegean basins, this summer marked the warmest ocean conditions over the past four decades. Despite a relatively lower number of marine heatwaves (MHWs) compared to previous warm years, the duration and cumulative intensity of these events in 2024 were the highest on record, reaching nearly twice the levels seen in 2018, which was the warmest until now. Intense MHWs were recorded, especially in the northern Aegean, with extensive biological consequences to ecosystems like the Thermaikos Gulf, a recognized MHW hotspot. The strong downward atmospheric heat fluxes in the summer of 2024, following an interannual increasing four-decade trend, contributed to the extreme warming of the water masses together with other met-ocean conditions such as lateral exchanges and vertical processes. The high temperatures were not limited to the surface but extended to depths of 50 m in some regions, indicating a deep and widespread warming of the upper ocean. Mechanisms typically mitigating SST rises, such as the Black Sea water (BSW) inflow and coastal upwelling over the eastern Aegean Sea, were weaker in 2024. Cooler water influx from the BSW decreased, as indicated by satellite-derived chlorophyll-a concentrations, while upwelled waters from depths of 40 to 80 m at certain areas showed elevated temperatures, likely limiting their cooling effects on the surface. Prolonged warming of ocean waters in a semi-enclosed basin such as the Mediterranean and its marginal sea sub-basins can have substantial physical, biological, and socioeconomic impacts on the AICS. This research highlights the urgent need for targeted monitoring and mitigation strategies to address the growing impact of MHWs in the region. Full article

Biological control of undesirable organisms and pathogens often involves the introduction of alien species into new regions. However, alien species themselves pose a potential threat to local ecosystems and economies. The Eastern mosquitofish Gambusia holbrooki is recognised as a dangerous invasive species, but despite this, it is still used for biological control of mosquito larvae, a potential vector of malaria plasmodium transmission to humans, on the Black Sea coast of the Caucasus. We focused on the species composition of helminths in this fish species. We detected adult nematodes Pseudocapillaria (Pseudocapillaria) tomentosa and cestodes Schyzocotyle acheilognathi (formely Bothriocephalus acheilognathi). The above nematode was observed for the first time in fish of the genus Gambusia. Importantly, the cestode S. acheilognathi, which we found in G. holbrooki, is the most successful invasive alien parasite species in freshwaters of the planet and is extremely hazardous to natural ecosystems and aquaculture as it can cause mass mortality of young fish. Thus, the current practice of transferring mosquitofish between water bodies on the Black Sea coast of the Caucasus to control mosquito larvae may contribute to the undesirable spread of a harmful quarantine fish parasite. Full article

Ornithogalum is a genus of wild herb species widely used as food and in traditional medicine. This study investigated some of the biologically active properties of Ornithogalum balansae grown in the eastern Black Sea region of Türkiye. The investigations were carried out using the methanolic extracts of the plant’s aerial and bulbs (B1 and B2) parts. The phenolic composition was examined as total phenolic content (TPC), total flavonoid content (TFC), and HPLC-PDA. The antioxidant capacity of the extracts was tested based on ferric reducing/antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging methods. The anti-proliferative activity was tested against metastatic cell lines H1299 and H209, non-small-cell lung A549, and fibroblast MRC-5 cell lines using MTT and trypan blue methods. Wound-healing and invasion chamber assays were used to determine the inhibitory effects of the extracts on migration and invasion, respectively. The extract of the aerial part contained a large number of phenolic substances and high antioxidant capacity. The extract exhibited a significant anti-proliferative effect on the human lung cancer cells (A549 and H209), with IC₅₀ values of 0.97 ± 0.04 and 1.06 ± 0.07 µg/mL, respectively. Moreover, the aerial part exhibited inhibition of migratory and invasive capacities in A549 cells at a concentration of 1.50 µg/mL. The findings associated with O. balansae suggest a promising therapeutic potential against lung cancer cells. Full article

The altitude of the habitat is one of the important regulators of species survival. Kaçkar Mountains National Park is located in the Eastern Black Sea region of Turkey. This is the first study on the benthic diatom flora of the high mountain lakes in Kaçkar Mountains National Park, which is situated between 2782 and 3075 m a.s.l. A total of 84 diatom species were identified from benthic communities of 15 habitats in summer (19 July, 28 August) and autumn (10 September) months of 2020. The genus Pinnularia (thirteen species) formed the basis of the taxonomic list, followed by Eunotia (five species), Navicula (five species), and Frustulia (four species) genera, respectively. The waters in all the studied lakes were fresh, low-saline, with low-alkaline or circumneutral pH and organically uncontaminated, as evidenced by prevailed bioindicator groups. Statistical methods and comparative floristic results confirm the role of the lake altitude for the diatom species distribution. The species richness of the studied lakes was higher in lakes with lower altitudes. The statistical approach also revealed the potential for an increase in the number of species in high mountain lakes if the study of the diatom flora of the Kachkar Mountains National Park is continued and the species composition of the lakes is replenished. Further studies will be needed to continue exploring this pattern. To protect studied high mountain lakes, their ecological conditions must be constantly monitored in the Kaçkar Mountains National Park. Full article

Since 2018, the authors have been working on reconstructing the exterior of the Great Pitiunt Roman fortress in 3D. This article presents the results of the visualization of the exterior and interior of the temple complex. During the study, the dimensions and plans of the site were analyzed, revealing discrepancies in various sources. To clarify the complex’s dimensions, aerial photography using UAV was conducted, and photogrammetric models, orthophoto, and digital surface models were created. The research also uncovered previously unrecorded architectural features. During the reconstruction of the temples, much attention was paid to the structural design of the buildings, connections and load distribution. Engineering calculations have been carried out for the clarification of the structural solutions. The article presents the results of a detailed reconstruction of the exterior, interior and structural features of Temple Nos. 1–4, based on preserved archaeological evidence, excavation results, contemporaneous Early Christian sites, and an analysis of the materials and technologies used at the time. The reconstruction of the mosaic floor of Temple No. 2 allowed a realistic visualization of the interior. Full article

The global climate change, driven by natural processes and increasing human activities, is especially significant for Georgia. The region is experiencing increases in temperature, desertification, redistribution of precipitation, and a rise in the frequency and severity of extreme weather events. Georgia’s complex topography and its proximity to the Black and Caspian seas make it essential to employ high-resolution regional climate models to evaluate future climate change risks. In this study, we examine the results of a high-resolution simulation of mean and extreme precipitation and temperature using the Abdus Salam International Centre for Theoretical Physics Regional Climate Model version 4.7.1 for the period 1985–2008, providing an initial evaluation of the model’s performance for the territory of Georgia. The model domain (1524 km; 2388 km) encompasses the entirety of Georgia’s territory and surrounding regions. The simulation, conducted at a 12 km horizontal grid spacing using ERA5 data as boundary conditions, indicates that the least discrepancy between observed and modeled average annual temperatures and precipitation, falling within a −1 to 1 °C and −200 to 200 mm range, respectively, was observed at most stations of eastern Georgia. The largest disparities between the model and observed average annual precipitation totals were noted along the Black Sea coast, in the Kolkheti Lowland, and in some high mountain stations in western Georgia. The most significant differences in average annual temperatures between the model and observations were observed in Ambrolauri, Mt. Sabueti, and Dedoplistskaro. For Georgia territory, such a long run with such a high resolution using ERA5 as boundary conditions was conducted for the first time. Overall, the modeling results are quite satisfactory, providing a solid basis for the successful utilization of the regional climate model RegCM4.7.1 with the selected parameterization for modeling monthly mean and extreme temperatures and precipitation in Georgia. Full article

Species of the Berberis genus, which are widely distributed naturally throughout the world, are cultivated and used for various purposes such as food, medicinal applications, and manufacturing dyes. Model-based machine learning is a language for specifying models, allowing the definition of a model using concise code, and enabling the automatic creation of software that implements the specified model. Maximum entropy (MaxEnt 3.4.1) is an algorithm used to model the appropriate distribution of species across geographical regions and is based on the species distribution model that is frequently also used in modeling the current and future potential distribution areas of plant species. Therefore, this study was conducted to estimate the current and future potential distribution areas of Berberis vulgaris in Türkiye for the periods 2041–2060 and 2081–2100, according to the SSP2 4.5 and SSP5 8.5 scenarios based on the IPSL-CM6A-LR climate change model. For this purpose, the coordinates obtained in the WGS 84 coordinate system were marked using the 5 m high spatial resolution Google Satellite Hybrid base maps, which are readily available in the 3.10.4 QGIS program, the current version of QGIS (Quantum GIS). The CM6A-LR climate model, the latest version of the IPSL climate models, was used to predict the species’ future distribution area. The area showed a high correlation with the points representing B. vulgaris, which is generally distributed in the Mediterranean and the central and eastern Black Sea regions of Türkiye, and the very suitable areas encompassed 45,413.82 km². However, when the SSP2 4.5 scenario was considered for the period 2041–2060, the areas very suitable for Berberis vulgaris comprised 59,120.05 km², and in the SSP2 4.5 scenario, very suitable areas were found to encompass 56,730.46 km² in the 2081–2100 period. Considering the SSP5 8.5 scenario for the period 2041–2060, the area most suitable for the B. vulgaris species is 66,670.39 km². In the SSP5 8.5 scenario, very suitable areas were found to cover 20,108.29 km² in the 2081–2100 period. Careful consideration of both the potential positive and negative impacts of climate change is essential, and these should be regarded as opportunities to implement appropriate adaptation strategies. The necessary conditions for the continued existence and sustainability of B. vulgaris—that is, areas with ecological niche potential—have been determined. Full article

The genetic composition of 5797 white oaks assigned in forest inventories as Quercus robur (3342), Quercus petraea (2090), Quercus pubescens (170), or as unspecified Quercus. spp. (195) sampled all over Europe were genotyped at 355 nuclear SNPs and 28 maternally inherited SNPs of the chloroplast and mitochondria. The sampling had a focus on Central and Eastern Europe, as well as the Black Sea and Caucasus region. Using a sparse nonnegative matrix factorization (snmf) algorithm, the nuclear genetic information was best represented by K = 4 different genetic clusters, whereas a principal component analysis visualized three different groups. The snmf run with K = 3 corresponded, for most individuals with the assignment in the forest inventories, to the three different species. The majority of the samples (88%) had an admixture coefficient q > 0.8 for one of the three species clusters, underlining the species integrity with a minor level of admixture. In contrast to Q. petraea, Q. robur and Q. pubescens showed a clear geographic genetic substructure. These large-scale within-species genetic structures were correlated to regionally variable levels of introgression between the species. For Q. petraea, introgression from Q. robur and Q. pubescens was less focused to particular regions, and this widespread inter-specific gene flow reduced the geographic genetic differentiation. The genetic variation at the maternally inherited SNPs led to 12 different haplotypes with a clear cross-species geographic pattern, further supporting the observation of significant hybridization and introgression among the species. Full article

The problem of shoaling on coastal structures is the result of an event that occurs as part of the natural cycle. In cases where shoaling cannot be detected or prevented, various economic and operational problems may arise and may cause disruptions. In this study, the complex coastal dynamic impact of shoaling on three sequential fishery coastal structures located within the borders of Rize province in the Eastern Black Sea region of Türkiye was examined in terms of bathymetric changes and sediment transport under the influence of the incident wave climate. The effects of these structures on each other were also investigated. With this aim, bathymetric measurements were carried out to examine the impact of waves on seabed erosion and deposition. A serious shoaling problem was identified at one of the harbor launches under investigation, where approximately 13,200 m³ of deposition occurs annually in a relatively small harbor launch area. Such physical problems are thought to be the result of shoaling, the selection of sites that are not viable for fishery-related coastal structures, or the wrong positioning of the breakwater. Full article

Liquid products obtained by the fast pyrolysis process applied to biomass can be used as chemical raw materials and liquid fuels. In this study, tea bush, walnut shell, and pine cone samples selected as biomass samples were obtained from Trabzon and Rize provinces in the Eastern Black Sea Region and used. When considered in terms of our region, the available biomass waste samples are easy to access and have a high potential in quantity. To employ them in the experimental investigation, these biomass samples were first ground, sieved to a particle size of 1.0 mm, and mixed. A fast pyrolysis process was applied to this obtained biomass mixture in a fixed-bed pyrolysis reactor. The effects of temperature, heating rate, and nitrogen flow rate on the product yields of the fast pyrolysis technique used on the biomass mixture are examined. A constant particle size of 1.0 mm, temperatures of 300, 400, 500, 600, and 750 °C, heating rates of 100, 250, 400, and 600 °C.min⁻¹, and flow rates of 50, 100, 200, and 300 cm³.min⁻¹ were used in tests on fast pyrolysis. The studies showed the 500 °C pyrolysis temperature, 100 °C min⁻¹ heating rate, and 50 cm³.min⁻¹ nitrogen flow rate gave the maximum liquid product yield. The liquid product generated under the most compelling circumstances is analyzed to determine moisture, calorific value, fixed carbon, ash, raw coke, and volatile matter. Additionally, the crude bio-oil heating value, measured at 5900 cal/g and produced under the most favorable pyrolysis circumstances, rose by around 40% compared to its starting material. The liquid product obtained from rapid pyrolysis experiments can be used as liquid fuel. The evaluation of the potential of chemical raw materials can be a subject of research in a different discipline since there are many chemical raw materials (glycerine, furfurals, cellulose and derivatives, carbonaceous materials, and so forth) in fast pyrolysis liquids. Full article