Search Results (11)

Three new phenanthridine peptide derivatives (19, 22, and 23) were synthesized to explore their potential as spectrophotometric probes for DNA and RNA. UV/Vis and circular dichroism (CD) spectra, mass spectroscopy, and computational analysis confirmed the presence of intramolecular interactions in all three compounds. Computational analysis revealed that compounds alternate between bent and open conformations, highlighting the latter’s crucial influence on successful polynucleotide recognition. Substituting one glycine with lysine in two regioisomers (22, 23) resulted in stronger binding interactions with DNA and RNA than for a compound containing two glycines (19), thus emphasizing the importance of lysine. The regioisomer with lysine closer to the phenanthridine ring (23) exhibited a dual and selective fluorimetric response with non-alternating AT and ATT polynucleotides and induction of triplex formation from the AT duplex. The best binding constant (K) with a value of 2.5 × 10⁷ M⁻¹ was obtained for the interaction with AT and ATT polynucleotides. Furthermore, apart from distinguishing between different types of ds-DNA and ds-RNA, the same compound could recognize GC-rich DNA through distinct induced CD signals. Full article

Cellulose derivatives, as edible coating for fruits and vegetables, have been broadly applied due to their availability, stability, solubility, safety, and low price. Therefore, this study was conducted to (1) extract cellulose from the banana plant rachis, (2) convert it into carboxymethyl (CMC), and (3) use the produced CMC as an edible coating to retard senescence and prolong the storage life of strawberry fruit. Preparation of CMC was accomplished by an etherification process, utilizing sodium hydroxide and monochloroacetic acid (MCA), with ethanol as a supporting medium. Characterization of CMC was done by analyzing the spectra of FTIR, degree of substitution (DS), ash content, CMC yield, water and oil holding capacity, in addition to physical characteristics. A storage study with CMC as an edible coating was conducted to investigate its impact on the shelf life of stored strawberry fruits. High purity food-grade CMC was successfully produced. CMC showed a yield of 156.25% with a DS of 0.78, a water holding capacity of 11.24 g/g, and an oil holding capacity of 1.60 g/g. The resulted CMC was well suited for edible coating preparation and was used effectively to prolong the shelf life of stored strawberry fruits at 22 °C to 6 days and to 16 days for that stored at 4 °C. Weight loss, total soluble solids (TSS), decay percentage, pH, anthocyanin content, ascorbic acid content, firmness, and sensory characteristics of CMC coated strawberry fruit stored at 4 °C were better than those stored at 22 °C. Thus, CMC edible coating prepared from banana rachis could be recommended as a potential postharvest treatment to delay postharvest senescence and maintain the quality of ambient and cold stored fruits. Full article

In this study, Hausa potato starch was subjected to single and dual modifications by HMT and citric acid. The parameters such as relative crystallinity, pasting, functional properties, and in vitro digestibility were studied. The XRD analysis showed an A-type diffraction pattern for the native starch and was unaffected by all modifications. The relative crystallinity and the gelatinization enthalpy significantly decreased (p ≤ 0.05) on dual modification. A new peak at 1724 cm⁻¹ was observed in the FT-IR spectra of citric acid-modified starch. The peak intensity became stronger in HMT followed by citric acid-modified starch, which was consistent with the results of degree of substitution. The native starch showed a peak viscosity of 3343 cP and significantly decreased on citric acid modification, and HMT treatment increased the effectiveness of the citric acid modification. The in vitro digestibility of Hausa potato starch was significantly affected by both single and dual modifications. The increased DS and RS content of the dual-modified starch suggests that the HMT served as a pre-treatment and favored the production of the citrate starch. The HMT-citric acid dual modification method was shown to modify the starch properties and could be used as a substitute for producing low glycaemic index foods. Full article

A water-soluble hydrolysate of silk fibroin (SF) (~30 kDa) was esterified with tocopherol, ergocalciferol, and testosterone to form SF aggregates for the controlled delivery of the anticancer drug camptothecin (CPT). Elemental analysis and ¹H NMR spectroscopy showed a degree of substitution (DS) on SF of 0.4 to 3.8 mol %. Yields of 58 to 71% on vitamins- and testosterone-grafted SF conjugates were achieved. CPT was efficiently incorporated into the lipophilic core of SF aggregates using a dialysis–precipitation method, achieving drug contents of 6.3–8.5 wt %. FTIR spectra and DSC thermograms showed that tocopherol- and testosterone-grafted SF conjugates predominantly adopted a β-sheet conformation. After the esterification of tyrosine residues on SF chains with the vitamin or testosterone, the hydrodynamic diameters almost doubled or tripled that of SF. The zeta potential values after esterification increased to about −30 mV, which favors the stability of aggregates in aqueous medium. Controlled and almost quantitative release of CPT was achieved after 6 days in PBS at 37 °C, with almost linear release during the first 8 h. MCF-7 cancer cells exhibited good uptake of CPT-loaded SF aggregates after 6 h, causing cell death and cell cycle arrest in the G2/M phase. Substantial uptake of the CPT-loaded aggregates into MCF-7 spheroids was shown after 3 days. Furthermore, all CPT-loaded SF aggregates demonstrated superior toxicity to MCF-7 spheroids compared with parent CPT. Blank SF aggregates induced no hemolysis at pH 6.2 and 7.4, while CPT-loaded SF aggregates provoked hemolysis at pH 6.2 but not at pH 7.4. In contrast, parent CPT caused hemolysis at both pH tested. Therefore, CPT-loaded SF aggregates are promising candidates for chemotherapy. Full article

The deep-space (DS) view spectra are used as a cold reference to calibrate the Hyperspectral Infrared Atmospheric Sounder (HIRAS) Earth scene (ES) observations. The DS spectra stability in the moving average window is crucial to the calibration accuracy of ES radiances. While in the winter and spring seasons, the HIRAS detector-3 DS view is susceptible to solar stray light intrusion when the satellite flies towards the tail of every descending orbit, and as a result, the measured DS spectra are contaminated by the stray light pseudo spectra, especially in the short-wave infrared (SWIR) band. The solar light intrusion issue was addressed on 13 December 2019 when the DS view angle of the scene selection mirror (SSM) was adjusted from −77.4° to −87°. As for the historic contaminated data, a correction method is applied to detect the anomalous data by checking the continuity of the DS spectra and then replace them with the proximate normal ones. The historic ES observations are recalibrated after the contaminated DS spectra correction. The effect of the correction is assessed by comparing the recalibrated HIRAS radiances with those measured by the Cross-track Infrared Sounder onboard the Suomi National Polar-orbiting Partnership Satellite (SNPP/CrIS) via the extended simultaneous nadir overpasses (SNOx) technique and by checking the consistency among the radiance data from different HIRAS detectors. The results show that the large biases of the radiance brightness temperature (BT) caused by the contamination are ameliorated greatly to the levels observed in the normal conditions. Full article

Starches rich in amylose are promising functional ingredients for calory-reduced foods. In this research, a high-amylose Japonica rice starch (amylose content 33.3%) was esterified with octenyl succinic anhydride (OSA) to improve the functional properties. The OSA-modified derivatives were evaluated for structure and functional properties, with OSA-modified normal Japonica rice starch (amylose content 18.8%) used as control. Fourier transform infrared spectra confirmed the introduction of OSA groups to starch. OSA modification made little change to morphology and particle size of high-amylose starch, but decreased the relative crystallinity and pasting temperature and increased the pasting viscosity, swelling power, emulsifying stability, and resistant starch (RS) content. The changes of properties were related to the degree of substitution (DS). Typically, OSA-modified high-amylose starch at DS of 0.0285 shows polyhedral-shape granules, with a volume-average particle diameter of 8.87 μm, peak viscosity of 5730 cp, and RS content of 35.45%. OSA-modified high-amylose starch had greater peak viscosity and RS content and lower swelling power than OSA-modified normal starch of similar DS, but the two kinds of derivatives did not have a significant difference in emulsifying stability. The OSA-modified high-amylose Japonica rice starch could be used as an emulsifier, thickener, and fat replacer in food systems. Full article

The effect of dual modification of corn starch, including hydrolysis and succinylation, were evaluated through peak viscosity (PV) analysis, differential scanning calorimetry (DSC), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR) and Raman spectroscopy. This dual modification was shown to increase the reaction efficiency (RE) and degree substitution (DS) compared with starches that were not subjected to acid hydrolysis pretreatment with a 44% and 45% increase respectively. After acid hydrolysis pretreatment, the surface of the corn starch granules exhibited exo-erosion and whitish points due to the accumulation of succinyl groups. The peak viscosity was reduced significantly with the acid hydrolysis pretreatment (between 3 and 3.5-fold decrease), which decreased the pasting temperature and peak time to 20 °C and 100 s respectively. In addition, the dual modification of corn starch altered certain thermal properties, including a reduction in the enthalpy of gelatinization (ΔH) and a higher range of gelatinization (around 6 °C), which may effectively improve industrial applications. Modifications on the FTIR spectra indicated that the dual modification affected the starch crystallinity, while the Raman spectra revealed that the dual modification disrupted the short-range molecular order in the starch. Rearrangement and molecular destabilization of the starch components promoted their granular amphiphilic properties. Full article

Resistant starch has gradually become a popular food component due to its beneficial physiological effects and heat resistance during processing. In this study, the structure, reaction mechanism, and digestibility of wrinkled pea starch with malic acid and heat–moisture treatment (HMT) are investigated. The degree of substitution (DS) of malate starch, HMT-malate starch, and malate-HMT starch was 0.164, 0.280, and 0.146, respectively. Malate starch remained in its complete particle form and pronounced birefringence was displayed. However, the malate-HMT starch sample was almost completely broken into pieces and lost the polarized cross. All modified starch samples had a decreased swelling power and a new peak at 1731–1741 cm⁻¹ shown by FTIR. From the ¹³C CP/MAS NMR (Cross Polarizatio/Magic Angle Spinning Nuclear Magnetic Resonance) spectra, all the modified starches had extra peaks at 38.5 ppm and 172.8 ppm. After esterification treatment, the resistant starch (RS) and slowly digestible starch (SDS) content of starch samples increased dramatically. The higher content of RS and lower enzymatic hydrolysis rate of the malate starch could be used to produce low-calorie foods and have potential health benefits. Full article

Cellulose was premodified by short-chain fatty acid anhydrides, such as acetic anhydride (CA), propionic anhydride (CP), and butyric anhydride (CB), followed by grafting of polyoxyethylene (2) hexadecyl ether (E₂C₁₆) using toluene-2,4-diisocyanate as a coupling agent. The feeding molar ratio of E₂C₁₆ and the anhydroglucose unit (AGU) was fixed at 4:1, and then a series of CA-g-E₂C₁₆, CP-g-E₂C₁₆, and CB-g-E₂C₁₆ copolymers were successfully prepared. The structures and properties of the copolymers were characterized using FTIR (fourier transform infrared spectra), ¹H-NMR (Proton nuclear magnetic resonance), DSC (Differential scanning calorimeter), POM (polarized light microscopy), TGA (thermogravimetric analysis) and WAXD (wide-angle X-ray diffraction). It was shown that with the anhydride/AGU ratio increasing, the degree of substitution (DS) value of E₂C₁₆ showed a trend of up first and then down. With the carbon chain length increasing, the DS value of E₂C₁₆ continuously increases. The phase transition temperature and thermal enthalpy of the copolymers increased with an increasing DS value of E₂C₁₆. When the ratio of CB/AGU was 1.5:1, the DS of E₂C₁₆ was up to the maximum value of 1.02, and the corresponding melting enthalpy and crystallization enthalpy were 32 J/g and 30 J/g, respectively. The copolymers showed solid–solid phase change behavior. The heat resistant temperature of cellulose-based solid–solid phase change materials was always higher than 270 °C. After the grafting reaction, the crystallinity of E₂C₁₆ decreased, while the crystal type was still hexagonal. Full article

A novel arginine-rich chitosan (CS) derivates mimicked cell penetration peptides; N-Arginine chitosan (N-Arg-CS) was prepared by two reaction methods involving activated L-arginine and the amine group on the chitosan. FTIR spectra showed that arginine was chemically coupled with CS. Elemental analysis estimated that the degrees of substitution (DS) of arginine in CS were 6%, 31.3% and 61.5%, respectively. The drug adefovir was chosen as model and its permeation flux across excised mice skin was investigated using a Franz diffusion cell. The results showed that the most effective enhancer was 2% (w/v) concentration of 10 kDa N-Arg-CS with 6% DS. At neutral pH, the cumulative amount of adefovir permeated after 12 hours was 2.63 ± 0.19 mg cm⁻² which was 5.83-fold more than adefovir aqueous solution. Meanwhile N-Arg-CS was 1.83, 2.22, and 2.45 times more effective than Azone, eucalyptus and peppermint, respectively. The obtained results suggest that N-Arg-CS could be a promising transdermal enhancer. Full article

Complexes of phosphated cornstarch and waxy cornstarch with casein were prepared and characterised. They were prepared from casein in defatted milk and corn and waxy corn starches phosphated to degree of substitution values (DS) of 0.0637 and 0.0968, respectively. The components were blended in starch to casein ratios of 2:1, 1:1, and 1:2, then precipitated with hydrochloric acid. Aqueous solubility, water binding capacity, IR spectra, and thermal analysis (thermogravimetry, TG, and differential thermogravimetry, DTG) of the precipitates revealed that they were not simple physical mixtures of the components. The components interact with one another electrostatically with involvement of the starch phosphate groups and the peptide bonds of casein as documented by the IR spectra. Because of their insolubility in 7 M aqueous urea solution they might also be considered as complexes in which the components were chemically bound. Enzymatic studies showed that they are biodegradable materials. Full article