Search Results (46)

Plants have played a key role in natural therapies for centuries, and their impact on the nervous system and the treatment of neurological disorders is of growing interest to scientists. Modern research confirms that plant substances can modulate neurotransmitters, reduce oxidative stress and support cognitive functions. Like humans, dogs also respond to plant compounds, which opens up new perspectives in veterinary medicine. The most well-known adaptogen is ginseng, and others include Siberian ginseng, Chinese magnolia vine, maral root, and golden root. These plants support the regulation of cortisol levels, neurotransmission and neuroplasticity. Although research on adaptogens in humans is advanced, there is still a lack of data on their effects on dogs. Further research is necessary to confirm their effectiveness and safety in animal therapy. Full article

With increasing concentrations of atmospheric greenhouse gases, the interaction between climate change and agriculture is receiving widespread attention as part of food security and sustainable human development. However, a comprehensive summary of knowledge in the field of climate change and agriculture from a scientometric perspective is still lacking. Here, we selected 25,872 papers related to climate change and agriculture from the Web of Science Core Collection database for the period 1985 to 2023 and used VOSviewer software to reveal the research status and trends. The main results were as follows: (1) the number of papers in this field showed a rapidly increasing trend after 2007, with a clear interdisciplinary characteristic; (2) The United States was the most influential country in this field with 6819 papers and 363,109 citations. China had the second highest number of papers (3722 papers), but the Chinese Academy of Sciences was the most influential institution with the most papers. On an author level, Pete Smith was the most influential; (3) All keywords were divided into four different research topics, such as the impact of climate change on agriculture, climate change mitigation and adaptation in agriculture, and crop growth in response to climate change. Among them, some keywords related to climate change adaptation were the most recent topics of interest in this field. These findings provide effective scientific references for relevant scientists and practitioners to better conduct future theoretical and practical research on climate change and agriculture. Full article

Popular photoluminescent (PL) nanomaterials, such as carbon dots, have attracted substantial attention from scientists due to their photophysical properties, biocompatibility, low cost, and diverse applicability. Carbon dots have been used in sensors, cell imaging, and cancer therapy. Leek seeds with anticancer, antimicrobial, and antioxidant functions serve as traditional Chinese medicine. However, leek seeds have not been studied as a precursor of carbon dots. In this study, leek seeds underwent a supercritical fluid extraction process. Leek seed extract was obtained and then carbonized using a dry heating method, followed by hydrolysis to form carbon dot micelles (CD-micelles). CD-micelles exhibited analyte-induced PL quenching against Co²⁺ through the static quenching mechanism, with the formation of self-assembled Co²⁺-CD-micelle sphere particles. In addition, CD-micelles extracted metal ion through liquid–liquid extraction, with removal efficiencies of >90% for Pb²⁺, Al³⁺, Fe³⁺, Cr³⁺, Pd²⁺, and Au³⁺. Moreover, CD-micelles exhibited ABTS•⁺ radical scavenging ability and cytotoxicity for cisplatin-resistant lung cancer cells. CD-micelles killed cisplatin-resistant small-cell lung cancer cells in a dose-dependent manner with a cancer cell survival rate down to 12.8 ± 4.2%, with a similar treatment function to that of cisplatin. Consequently, CD-micelles functionalized as novel antioxidants show great potential as anticancer nanodrugs in cancer treatment. Full article

Gene editing contributes to enhancing food security through the creation of novel foods. However, public perception of gene-edited (GE) foods is crucial to their acceptance and adoption. This study expanded the knowledge–attitude–practice model and constructed an integrated framework comprising four dimensions: demographic factors, scientific literacy and beliefs, social trust, and perceptions of gene technology, aimed at explaining the public’s attitudes toward GE foods. A questionnaire survey was conducted (N = 649), revealing a positive attitude toward GE foods, with over 80% expressing a certain willingness to pay (WTP) for them. Factors such as income level, subjective knowledge, scientific beliefs, trust in scientists, trust in government, and trust in national technological capabilities and perceived benefits positively correlated with WTP. Conversely, objective knowledge, perceived risks, and perceived ethical concerns were negatively correlated with WTP. The impact of objective knowledge on attitudes toward GE foods demonstrated a significant, nonlinear relationship. Additionally, it is noteworthy that the Chinese public currently exhibits relatively low trust in national technological capabilities, necessitating vigilance against the emergence of conspiracy theories akin to those surrounding genetically modified foods. This research contributes theoretical insights into the public communication of GE foods. Full article

Inflammatory bowel disease (IBD) is a chronic and recurrent intestinal inflammatory disease, mainly including Crohn’s disease (CD) and ulcerative colitis (UC). In recent years, the incidence and prevalence of IBD have been on the rise worldwide and have become a significant concern of health and a huge economic burden on patients. The occurrence and development of IBD involve a variety of pathogenic factors. The changes in short-chain fatty acids (SCFAs) are considered to be an important pathogenic mechanism of this disease. SCFAs are important metabolites in the intestinal microbial environment, which are closely involved in regulating immune, anti-tumor, and anti-inflammatory activities. Changes in metabolite levels can reflect the homeostasis of the intestinal microflora. Recent studies have shown that SCFAs provide energy for host cells and intestinal microflora, shape the intestinal environment, and regulate the immune system, thereby regulating intestinal physiology. SCFAs can effectively reduce the incidence of enteritis, cardiovascular disease, colon cancer, obesity, and diabetes, and also play an important role in maintaining the balance of energy metabolism (mainly glucose metabolism) and improving insulin tolerance. In recent years, many studies have shown that numerous decoctions and natural compounds of traditional Chinese medicine have shown promising therapeutic activities in multiple animal models of colitis and thus attracted increasing attention from scientists in the study of IBD treatment. Some of these traditional Chinese medicines or compounds can effectively alleviate colonic inflammation and clinical symptoms by regulating the generation of SCFAs. This study reviews the effects of various traditional Chinese medicines or bioactive substances on the production of SCFAs and their potential impacts on the severity of colonic inflammation. On this basis, we discussed the mechanism of SCFAs in regulating IBD-associated inflammation, as well as the related regulatory factors and signaling pathways. In addition, we provide our understanding of the limitations of current research and the prospects for future studies on the development of new IBD therapies by targeting SCFAs. This review may widen our understanding of the effect of traditional medicine from the view of SCFAs and their role in alleviating IBD animal models, thus contributing to the studies of IBD researchers. Full article

The 2023 International African Swine Fever Workshop (IASFW) took place in Beijing, China, on 18–20 September 2023. It was jointly organized by the U.S.-China Center for Animal Health (USCCAH) at Kansas State University (KSU) and the Chinese Veterinary Drug Association (CVDA) and sponsored by the United States Department of Agriculture Foreign Agricultural Service (USDA-FAS), Harbin Veterinary Research Institute, and Zoetis Inc. The objective of this workshop was to provide a platform for ASF researchers around the world to unite and share their knowledge and expertise on ASF control and prevention. A total of 24 outstanding ASF research scientists and experts from 10 countries attended this meeting. The workshop included presentations on current ASF research, opportunities for scientific collaboration, and discussions of lessons and experiences learned from China/Asia, Africa, and Europe. This article summarizes the meeting highlights and presents some critical issues that need to be addressed for ASF control and prevention in the future. Full article

Plastic waste management has emerged as an urgent global challenge, that has attracted significant attention from researchers, policymakers, and stakeholders. Conducting a bibliometric study on this issue aims to provide a holistic and in-depth bibliometric mapping and analysis of plastic waste management, carefully traced through the Web of Science. Expansive data comprising scientific production by researchers, country/region contributions, affiliations, author references, and topical trends were meticulously scrutinized using the VOSviewer software (version 1.6.15). The exploration of bibliometric studies in plastic waste management is still limited, and this forms the basis of our commitment to explore this field in more depth. The findings in this study showed a progressive increase in the number of published works from 1992 to 2022, using the main keyword “plastic waste management” and 13,103 documents were found that were further analyzed. This demonstrates the profound impact of Chinese scientists on plastic waste management research, as evidenced by their prevalence as top affiliated authors. Given the increasing concern and heightened government and societal attention towards plastic pollution and water contamination, effective waste management, with a particular focus on plastic waste, emerged as the most influential keyword. This comprehensive analysis contributes to the existing literature by highlighting key trends and the urgent need for concerted efforts to address the challenges posed by plastic waste. Full article

Continuous cropping is the primary cultivation method in Chinese facility agriculture, and the challenge of it stands as a global issue in soil remediation. Growing tomatoes continuously on the same plot for an extended period can result in outbreaks of tomato bacterial wilt. It is caused by the soil-borne bacterium Ralstonia solanacearum, a widespread plant pathogen that inflicts considerable damage on economically significant crops worldwide. Simultaneously, this plant pathogen proves extremely resilient, as it can adhere to plant residues and persist through the winter, continuing to infect plants in subsequent years. Scientists have dedicated considerable efforts towards finding effective methods to manage this disease. This article delineates the characteristics of tomato bacterial wilt and the various types of pathogenic bacteria involved. It systematically reviews the progress in research aimed at controlling tomato bacterial wilt, encompassing both physical and biological aspects concerning soil and plants. Emphasis is placed on the principles and current applications of these control measures, alongside proposed improvements to address their limitations. It is anticipated that the future of tomato bacterial wilt control will revolve around the development of a novel environmental protection system and efficient control strategies, focusing on microecological management and enhancing tomato resistance against bacterial wilt through breeding. Full article

The growing negative anthropogenic impact on the environment causes scientific interest in the problems of water management. The increasing number of publications in this scientific field requires their intellectual systematization. The purpose of this study is to conduct a bibliometric review of scientific publications related to water management issues in the context of solving the problems of increasing the sustainability of the socio-economic development of a region for better understanding of current research trends. To achieve this goal, bibliometric analysis using the VOSviewer software product (Manual for VOSviewer version 1.6.17) was used. The international database Scopus was taken as the source of information. This study examined 10,208 articles on water management issues from 2012 to 2022. The basic criterion for including a publication in the selection was that the topic of the work belongs to the subject areas of economics, econometrics and finance and business, management and accounting. As a result of the analysis, it was determined that the problems of water resources management have not lost their popularity in the global research community and the research methodology is evolving towards the concept of “water–energy–food”. The centers of knowledge forming the vector of scientific research are the USA and the Netherlands; however, in recent years, the research of Chinese scientists has become increasingly important. It is concluded that the potential for the development of research in the field of water resources management in the context of solving the problems of the sustainable development of regions is associated with the search for opportunities for revealing the synergy of intersectoral interactions while taking into account their sectoral and regional specifics. Full article

RNA viruses are often cited as a significant factor affecting the populations of both domestic honey bees and wild pollinators. To expedite the development of effective countermeasures against these viruses, a more comprehensive understanding of virus biology necessitates extensive collaboration among scientists from diverse research fields. While the infectious virus clone is a robust tool for studying virus diseases, the current methods for synthesizing infectious clones of bee-infecting RNA viruses entail the in vitro transcription of the viral genome RNA in 8–10 kb, presenting challenges in reproducibility and distribution. This article reports on the synthesis of an infectious clone of the Chinese variant sacbrood virus (SBV) using a DNA plasmid containing an Autographa californica multiple nucleopolyhedrovirus (AcMNPV) immediate-early protein (IE1) promoter to trigger transcription of the downstream viral genome within hosts. The results demonstrate that the IE1-SBV plasmid can synthesize SBV clones in a widely used lepidopteran immortal cell line (Sf9) and honey bee pupae. Furthermore, the negative strand of the clone was detected in both Sf9 cells and honey bee pupae, indicating active infection and replication. However, the transfection of Sf9 cells was observed in only a limited proportion (less than 10%) of the cells, and the infection did not appear to spread to adjacent cells or form infective virions. The injection of honey bee pupae with 2500 ng of the IE1-SBV plasmid resulted in high infection rates in Apis cerana pupae but low rates in A. mellifera pupae, although the dosage was comparatively high compared with other studies using in vitro transcribed viral RNA. Our findings suggest that the synthesis of bee-infecting RNA viruses using DNA plasmids is feasible, albeit requiring additional optimization. However, this method holds substantial potential for facilitating the production of clones with various sequence modifications, enabling the exploration of viral gene functions and biology. The ease of distributing infectious clones in DNA plasmid form may foster collaboration among scientists in applying the clone to bee biology, ecology, and behavior, ultimately offering a comprehensive approach to managing virus diseases in the future. Full article

Detailed information about mangroves is crucial for ecological and environmental protection and sustainable development. It is difficult to capture small patches of mangroves from satellite images with relatively low to medium resolution. In this study, high-resolution (0.8–2 m) images from Chinese GaoFen (GF) and ZiYuan (ZY) series satellites were used to map the distribution of mangroves in coastal areas of Guangdong Province, China. A deep-learning network, U²-Net, with attention gates was applied to extract multi-scale information of mangroves from satellite images. The results showed that the attention U²-Net model performed well on mangrove classification. The overall accuracy, precision, and F1-score values were 96.5%, 92.0%, and 91.5%, respectively, which were higher than those obtained from other machine-learning methods such as Random Forest or U-Net. Based on the high-resolution mangrove maps generated from long satellite image time series, we also investigated the spatiotemporal evolution of the mangrove forest in Shuidong Bay. The results can provide crucial information for government administrators, scientists, and other stakeholders to monitor the dynamic changes in mangroves. Full article

Understanding the grassland ecosystem is crucial for improving grassland ecosystem functions and services such as climate regulation, water and soil conservation, carbon sequestration, and biodiversity and gene pool maintenance. However, a systematic and comprehensive review of the relevant literature is still unclear and lacking. The VOSviewer software and cluster analysis were used to visually analyze and perform dimension reduction classification on the 27,778 studies related to grassland ecosystem research based on the Web of Science database. The number of publications targeting grassland ecosystem increased rapidly from 2006 to 2021. Ecology, agronomy, and environmental science were the most popular research categories, and the top journal sources were Remote Sensing, Journal of Ecology, and Ecology and Evolution. The leading publishing countries were the United States, China, and Germany. The top three institutions were the Chinese Academy of Sciences, the University of Chinese Academy of Sciences, and Colorado State University. Cooperation between different countries and institutions had increased. Keyword co-occurrence network analysis showed that Biodiversity, Vegetation and Conservation were the most popular study areas, grassland management, climate change, land use pattern, and ecosystem impact were the hot research topics. All studies could be divided into three categories by cluster analysis: grassland ecological characteristics including basic physicochemical properties, vegetation community characteristics, aboveground and belowground biomass, and soil structural quality of grassland; driving mechanisms that demonstrated effects of human activities and climate change on grassland ecosystem function; and grassland ecosystem services that focused the influences of different grassland management strategies on ecological services, animal welfare and human well-being. The three topic categories of reviewed studies were interrelated and consistent with each other, and the performances were progressive. This paper reviewed the trend evolution through keyword hotspots and analyzed the future research directions to provide an important reference for scientists to better respond to the balance of herbage and sustainable utilization of grassland and maintenance of ecological security. Full article

Antibacterial resistance has become one of the most serious problems threating global health. To overcome this urgent problem, many scientists have paid great attention to developing new antibacterial drugs from natural products. Hence, for exploring new antibacterial drugs from Chinese medicine, a series of experiments were carried out for verifying and elucidating the antibacterial activity and mechanisms of madecassic acid (MA), which is an active triterpenoid compound isolated from the traditional Chinese medicine, Centella asiatica. The antibacterial activity was investigated through measuring the diameter of the inhibition zone, the minimum inhibitory concentration (MIC), the growth curve, and the effect on the bacterial biofilm, respectively. Meanwhile, the antibacterial mechanism was also discussed from the aspects of cell wall integrity variation, cell membrane permeability, and the activities of related enzymes in the respiratory metabolic pathway before and after the intervention by MA. The results showed that MA had an inhibitory effect on eight kinds of pathogenic bacteria, and the MIC values for Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilis, and Bacillus megaterium were 31.25, 62.5, 250, 125, 62.5, and 62.5 µg/mL, respectively. For instance, 31.25 µg/mL MA could inhibit the growth of Staphylococcus aureus within 28 h. The antibacterial mechanism experiments confirmed that MA could destroy the integrity of the cell membrane and cell wall of Staphylococcus aureus, causing the leakage of macromolecular substances, inhibiting the synthesis of soluble proteins, reducing the activities of succinate dehydrogenase and malate dehydrogenase, and interacting with DNA, leading to the relaxation and ring opening of supercoiled DNA. Besides, the activities of DNA topoisomerase I and II were both inhibited by MA, which led to the cell growth of Staphylococcus aureus being repressed. This study provides a theoretical basis and reference for the application of MA in the control and inhibition of food-borne Staphylococcus aureus. Full article

Escherichia albertii is an emerging, enteric pathogen of significance. It was first isolated in 2003 from a pediatric diarrheal sample from Bangladesh. In this study, a comprehensive in silico strategy was followed to first list out antibiotic-resistant genes from core, accessory and unique genome fractions of 95 available genomes of E. albertii. Then, 56 drug targets were identified from the core essential genome. Finally, ZipA, an essential cell division protein that stabilizes the FtsZ protofilaments by cross-linking them and serves as a cytoplasmic membrane anchor for the Z ring, was selected for further downstream processing. It was computationally modeled using a threading approach, followed by virtual screening of two phytochemical libraries, Ayurvedic (n = 2103 compounds) and Traditional Chinese Medicine (n = 36,043 compounds). ADMET profiling, followed by PBPK modeling in the central body compartment, in a population of 250 non-diseased, 250 cirrhotic and 250 renally impaired people was attempted. ZINC85624912 from Chinese medicinal library showed the highest bioavailability and plasma retention. This is the first attempt to simulate the fate of natural products in the body through PBPK. Dynamics simulation of 20 ns for the top three compounds from both libraries was also performed to validate the stability of the compounds. The obtained information from the current study could aid wet-lab scientists to work on the scaffold of screened drug-like compounds from natural resources and could be useful in our quest for therapy against antibiotic-resistant E. albertii. Full article