Search Results (1,833)

Directive (EU) 2024/3019 on urban wastewater treatment requires municipal wastewater treatment plants (WWTPs) to achieve energy neutrality by 2045. This study assessed the energy efficiency of a WWTP in central Poland over eight years (2015–2022), considering influent variability, electricity use and cost, and biogas recovery. The facility served 41,951–44,506 inhabitants, with treated wastewater volumes of 3.08–3.93 million m³/year and a real population equivalent (PE) of 86,602–220,459. Over the study period, the specific energy demand remained stable at 0.92–1.20 kWh/m³ (average 1.04 ± 0.09 kWh/m³), equivalent to 17.4–36.3 kWh/PE∙year. Energy efficiency indicators (EEIs) per pollutant load removed averaged 1.12 ± 0.28 kWh/kgBODrem, 0.53 ± 0.12 kWh/kgCODrem, 1.18 ± 0.36 kWh/kgTSSrem, 12.1 ± 1.5 kWh/kgTNrem, and 62.3 ± 11.7 kWh/kgTPrem. EEI per cubic meter of treated wastewater proved to be the most reliable metric for predicting energy demand under variable influent conditions. Electricity costs represented 4.48–13.92% of the total treatment costs, whereas co-generation from sludge-derived biogas covered 18.1–68.4% (average 40.8 ± 13.8%) of the total electricity demand. Recommended pathways to energy neutrality include co-digestion with external substrates, improving anaerobic digestion efficiency, integrating photovoltaics, and optimizing electricity use. Despite fluctuations in influent quality and load, the ultimate effluent quality consistently complied with legal requirements, except for isolated cases of exceeded phosphorus levels. Full article

Background: Communication difficulty restricts education, healthcare, and social participation, yet population-level data for Saudi Arabia have been scarce. This study analysed the 2017 Saudi National Disability Survey to estimate prevalence, describe severity and demographic patterns, and identify factors linked to these difficulties. Objectives: We aimed to estimate national and regional prevalence, assess severity, and gender differences, and identify socio-demographic and disability-related correlates. Methods: A cross-sectional, two-stage stratified cluster sample of 33,575 households (weighted N = 20,408,362 citizens) provided self-reported data on communication difficulty and socio-demographics. Weighted frequencies described prevalence and multivariable logistic regression identified independent correlates. Results: Among all Saudi citizens, 7.1% reported at least one functional difficulty, and of this group 15.7%—equivalent to 1.1% of the total population (n = 226,510)—had a communication difficulty; within that communication difficulty stratum, (n = 185,508) (0.9% of all citizens) experienced it alongside additional impairments, whereas (n = 41,002) (0.2% of all citizens) reported communication difficulty in isolation. The communication difficulties exhibit significant regional variation, ranging from 0.45% in Najran to 1.55% in Aseer. Most cases were classified as being associated with some difficulty (72%); females were over-represented in the extreme category despite a modest male excess overall (adjusted odds ratio [AOR] = 1.09). Higher education, married status, and bilateral first-cousin marriage (AOR = 1.22) were associated with greater risk. Chronic disease (44%) and perinatal causes (13%) predominated, and 84% of cases co-occurred with at least one other disability. Independent predictors included a long duration (AOR = 4.18), disease or delivery-related cause, and consanguinity. Conclusions: Findings highlight geographically clustered need, genetic risk factors, and substantial multimorbidity, indicating the importance of region-specific screening, premarital counselling, and integrated rehabilitation within chronic disease services. Full article

This study investigates the combustion behavior of H₂–CH₄ mixtures with oxygen-enriched air, achieved through injecting ozone (O₃) into the air intake of the burner fan. The motivation for this approach lies in the high reactivity of hydrogen compared to methane, with the aim of promoting a more favorable oxidizing environment for overall combustion. The research combines theoretical analysis with experimental validation using a diffusion-type burner operating at a fuel flow rate of 1.2 Nm³/h. For this flow rate, the ozone injection led to an equivalent O₂ concentration of approximately 21.7%. At this enrichment level, flame temperature was calculated to increase by 70–90 °C. The burner was specifically designed for the diffusion combustion of H₂–CH₄ mixtures and features three fuel injection nozzles, each surrounded by five air inlets. Experiments employed premixed H₂-CH₄ gas cylinders (Linde) with hydrogen concentrations of 20% and 30%, respectively. The results confirmed slight combustion intensification due to elevated oxygen concentration, with no issues related to flame stability or pulsations observed. Core flame temperature and flue gas emissions, including CO₂, were measured. The results support the further development of this combustion technology by increasing the allowable oxygen concentration limit. Full article

Although laboratory testing to assess aerobic capacity has been a ‘gold standard’ in sports science, its high costs and time-consuming protocols may not be feasible for monitoring and tracking progress in limited conditions. In dancesport athletes, several field-based aerobic tests have been proposed, but the majority of them have been developed for ballet or contemporary dancers at the individual level, while the data among dance couples engaging in standard dance styles is lacking. Therefore, the main purpose of this study was to validate a newly developed Ballroom Aerobic Test (BAT) protocol against objective methods. Twelve standard dancesport couples (age: 20.4 ± 3.9 years; height: 172.1 ± 8.7 cm; weight: 60.1 ± 9.4 kg) with 8.2 ± 3.4 years of training and competing experience participated in this study. Ventilatory and metabolic parameters were generated using the MetaMax^® 3B portable gas analyzer (the BAT), while the KF1 (an increase in speed by 0.5 km * h⁻¹ by every minute) and Bruce protocols were followed in laboratory-based settings on the running ergometer. Large to very large correlations were obtained between the BAT and KF1/Bruce protocols for the absolute maximal oxygen uptake (VO₂max; r = 0.88 and 0.87) and relative VO₂max (r = 0.88 and 0.85), respiratory exchange ratio (RER; r = 0.78 and 0.76), expiratory ventilation (VE; r = 0.86 and 0.79), tidal volume (VT; r = 0.75; 95% CI = 0.57–0.87; p < 0.001), ventilatory equivalent for O₂ (VE/VO₂; r = 0.81 and 0.80) and CO₂ (VE/VCO₂; r = 0.78 and 0.82), and dead space (VD/VT; r = 0.70 and 0.74). The Bland–Altman plots indicated no systematic and proportional biases between the BAT and KF1 protocols (standard error of estimate; SEE = ± 3.36 mL * kg⁻¹ * min⁻¹) and the BAT and Bruce protocols (SEE = ± 3.75 mL * kg⁻¹ * min⁻¹). This study shows that the BAT exhibits satisfactory agreement properties against objective methods and is a valid dance protocol to accurately estimate aerobic capacity in dancesport athletes participating in standard dance styles. Full article

To explore strategies for the safe utilization of farmland co-contaminated with cadmium (Cd) and lead (Pb), this field study systematically evaluated the impacts of humic acid (HA) and potassium fulvate (PF) at different application rates (0, 1500, 3000, and 4500 kg·ha⁻¹) on the growth, yield, and translocation of Cd and Pb within the soil–plant system of maize (Zea mays L.). The results showed that while HA and PF did not significantly alter total soil Cd and Pb concentrations, they markedly reduced their bioavailable fractions. This mitigation of heavy metal phytotoxicity significantly promoted maize growth and yield, with the high-dose HA treatment increasing yield by a maximum of 32.9%. Both amendments dose-dependently decreased Cd and Pb concentrations, bioconcentration factors (BCF), and translocation factors (TF) in all maize tissues, particularly in the grains. At equivalent application rates, PF was slightly more effective than HA in reducing heavy metal concentrations in the grains. Notably, a significant positive correlation was observed between Cd and Pb concentrations across all plant parts, confirming a synergistic accumulation and translocation mechanism. This synergy provides a physiological explanation for the broad-spectrum immobilization efficacy of these humic substances. In conclusion, applying HA and PF presents a dual-benefit strategy for increasing yield and reducing risks in Cd- and Pb-contaminated farmlands. This study proposes a differentiated application approach: PF is the preferred option when ensuring food-grade safety is the primary goal, whereas high-dose HA is more advantageous for maximizing yield in soils with low-to-moderate contamination risk. Full article

The nominal compositions of Fe₆₅Co_10−xNi_10−xCr₁₅Si_2x (x = 1, 2, and 3 at.%) medium-entropy alloys (MEAs) were designed and fabricated by vacuum arc melting. Their microstructure, hardness, and mechanical properties were systematically characterized. Corrosion behavior was evaluated in 3.5 wt.% NaCl solution by potentiodynamic polarization and electrochemical impedance spectroscopy. The investigated MEAs exhibit a dual-phase microstructure composed of face-centered cubic (FCC) and body-centered-cubic (BCC) phases. With increasing Si content, yield strength and ultimate tensile strength increase, while uniform elongation decreases. Hardness also increases with increasing Si content. For the x = 3 MEA, the yield strength, ultimate tensile strength, and hardness of are ~518 MPa, ~1053 MPa, and 262 ± 4.8 HV, respectively. The observed strengthening can be primarily attributed to solid solution strengthening effect by Si. Polarization curves indicate that the x = 3 MEA exhibits the best corrosion resistance with the lowest corrosion current density ((0.401 ± 0.19) × 10⁻⁶ A × cm⁻²) and corrosion rate ((4.65 ± 0.19) × 10^–2 μm × year⁻¹)). Equivalent electric circuit analysis suggests the formation of a stable passive oxide film on the MEAs. This conclusion is supported by the capacitive behavior, high impedance values (> 10⁴ Ω cm²) at low frequencies, and phase angles within a narrow window of 80.05°~80.64° in the medium-frequency region. The passive-film thickness was calculated and the corrosion morphology was analyzed by SEM. These results provide a reference for developing high-strength, corrosion-resistant, medium-entropy alloys. Full article

Background/Objectives: Our objective was to evaluate the clinical feasibility and sustainability of an awake breast conserving surgery (BCS) protocol integrating opioid-free anesthesia, telemedicine, and environmental sustainability compared to standard care. Methods: A prospective, monocentric, non-inferiority randomized controlled trial (133.24 CET2 ptv; NCT06624917) named Breast Green Surgery (BuGS) was planned. Women aged 18–75 years eligible for level I BCS were randomized to either the BuGS group—comprising opioid-free monitored anesthesia care (MAC), a telehealth follow-up, and intraoperative waste reduction—or the control. The primary endpoint was postoperative pain at rest (PPR) and during movement (PPD) both at 24 h, measured with the Numeric Pain Rating Scale (NPRS). Secondary exploratory endpoints included SF-36, PSQ-18, TSQ test, LOS, and KgCO₂ equivalent (KgCO₂e) from surgical waste, patient transport, and complications. The interim analysis included patients completing a 30-day follow-up by 31 December 2024. Results: A total of 45 patients were enrolled (BuGS n = 18, control n = 27). While disproportionate, no significant differences were observed in PPR/PPD at 24 h (4.75 (3.725–5.875) vs. 4.5 (4.15–5.35); p = 0.626; 4 (3.10–4.75) vs. 4.6 (3.90–5.2); p = 0.130), confirmed using group ANOVA analysis (p = 0.515; p = 0.779, respectively). The BuGS group reported a reduced surgical room occupation (80.03 (64.84–101.87) vs. 133.23 (95.47–144.25) min; p = 0.002) and length of stay (0 (0–1) vs. 1 (1–2); p = 0.0001), without hospital unplanned admissions. Reduced KgCO₂e emissions from waste disposal were reported, with no difference in SF-36, PSQ-18, and complication rates. Conclusions: If confirmed after complete accrual, BuGS could potentially promote a clinically equivalent, environmentally sustainable, and hospital efficient surgery without affecting the QoL of our patients. Further multicentric validation is warranted. Full article

Agrivoltaics (APV) mitigates land-use competition between photovoltaic installations and agricultural activities, thereby supporting multifaceted policy objectives in energy transition and sustainability. The availability of organic residuals from agrifood practices may also open the way to their energy valorization. This paper examines a small-scale farm in the Basilicata Region, southern Italy, to investigate the potential installation of an APV plant or a combined APV and bioenergy system to meet the electrical needs of the existing processing machinery. A dynamic numerical analysis is performed over an annual cycle to properly size the storage system under three distinct APV configurations. The panel shadowing effects on the underlying crops are quantified by evaluating the reduction in incident solar irradiance during daylight and the consequent agricultural yield differentials over the life period of each crop. The integration of APV and a biomass-powered cogenerator is then considered to explore the possible off-grid farm operation. In the sole APV case, the single-axis tracking configuration achieves the highest performance, with 45.83% self-consumption, a land equivalent ratio (LER) of 1.7, and a payback period of 2.77 years. For APV and bioenergy, integration with a 20 kW cogeneration unit achieves over 99% grid independence by utilizing a 97.57 kWh storage system. The CO₂ emission reduction is 49.6% for APV alone and 100% with biomass integration. Full article

Despite numerous research efforts and several effective vaccines and therapies developed against coronavirus disease 2019 (COVID-19), drug repurposing remains an attractive alternative approach for treatment of SARS-CoV-2 variants and other viral infections that may emerge in the future. Cellular polyamines support viral propagation and tumor growth. Here we tested the antiviral activity of two polyamine metabolism-targeting drugs, an irreversible inhibitor of polyamine biosynthesis, α-difluoromethylornithine (DFMO), and a non-steroidal anti-inflammatory drug (NSAID), Sulindac, which have been previously evaluated for colon cancer chemoprevention. The drugs were tested as single agents and in combination in the human Calu-3 lung adenocarcinoma and Caco-2 colon adenocarcinoma cell lines and the K18-hACE2 transgenic mouse model of severe COVID-19. In the infected human cell lines, the DFMO/Sulindac combination significantly suppressed SARS-CoV-2 N1 Nucleocapsid mRNA by interacting synergistically when cells were pretreated with drugs and additively when treatment was applied to the infected cells. The Sulindac alone and DFMO/Sulindac combination treatments also suppressed the expression of the viral Spike protein and the host angiotensin-converting enzyme 2 (ACE2). In K18-hACE2 mice, the antiviral activity of DFMO and Sulindac as single agents and in combination was tested as prophylaxis (drug supplementation started 7 days before infection) or as treatment (drug supplementation started 24 h post-infection) at the doses equivalent to patient chemoprevention trials (835 ppm DFMO and 167 ppm Sulindac). The drugs’ antiviral activity in vivo was evaluated by measuring the clinical (survival rates and clinical scores), viral (viral load and virus infectivity), and biochemical (plasma polyamine, Sulindac, and Sulindac metabolite levels) endpoints. Prophylaxis with DFMO and Sulindac as single agents significantly increased survival rates in the young male mice (p = 0.01 and p = 0.027, respectively), and the combination was effective in the aged male mice (p = 0.042). Young female mice benefited the most from the prophylaxis with Sulindac alone (p = 0.001) and the DFMO/Sulindac combination (p = 0.018), while aged female mice did not benefit significantly from any intervention. Treatment of SARS-CoV-2-infected animals with DFMO or/and Sulindac did not significantly improve their survival rates. Overall, our studies demonstrated that DFMO and Sulindac administration as the prophylaxis regimen provided strong protection against the lethal outcome of SARS-CoV-2 infection and that male mice benefited more from the polyamine-targeted antiviral treatment than female mice. Our findings underscore the importance of evaluation of the antiviral activity of the drugs in the context of sex and age. Full article

Heavy metal contamination in soil poses significant ecological risks, particularly within agricultural and forest ecosystems. This study evaluates the bioaccumulation of heavy metals (Cr, Co, Ni, Cu, Zn, As, Mo, Pb) by the ground beetle Carabus coriaceus Linnaeus, 1758, across contrasting Croatian ecosystems, with a focus on the role of soil pH in shaping metal dynamics. Concentrations in soils (0–30 and 30–60 cm) and beetle tissues were measured using portable X-ray fluorescence (pXRF), which provides total concentrations; inferences on bioavailability were based on soil properties such as pH and organic matter. Orchard soils showed higher Cu (49.9 mg/kg), Mo (10.3 mg/kg), and Ni (32.5 mg/kg), whereas forest soils contained elevated Zn (105.6 mg/kg), Pb (84.5 mg/kg), As (29.7 mg/kg), and Co (16.3 mg/kg). Beetles accumulated up to 481.0 mg/kg Zn at the orchard and 90.0 mg/kg Cu at the forest site. Bioaccumulation factors exceeded 1.0 for Co, Cu, and Zn, with particularly high values for Zn (2.20–5.75) suggesting both site-specific availability and possible physiological regulation. Soil and beetle analyses were complementary rather than equivalent: soils indicated total load, while beetles reflected biologically relevant fractions. C. coriaceus, therefore, represents a sensitive bioindicator, suitable for biodiversity-based soil contamination monitoring. Full article

The increasing generation of agricultural waste poses both environmental and economic challenges, particularly in rural areas with limited infrastructure. Anaerobic digestion has emerged as a sustainable alternative, enabling the valorization of waste and the production of biogas and biofertilizer. This study evaluated the economic and environmental gains of mono- and co-digestion of equine manure and vegetable waste using biodigesters of different capacities across four simulated projects—Project 1 (15 m² biodigester with monodigestion), Project 2 (15 m² biodigester with co-digestion), Project 3 (20 m² biodigester with monodigestion), and Project 4 (20 m² biodigester with co-digestion). Economic feasibility was assessed through indicators such as Net Present Value (NPV), Internal Rate of Return (IRR), Modified IRR (MIRR), Profitability Index (PI), Benefit-Cost Ratio (B/C), Discounted Payback Period, sensitivity analysis, and Monte Carlo simulation, adopting a Minimum Attractiveness Rate (MAR) of 6.43% per year. Environmental benefits were estimated based on the annual reduction of CO₂ equivalent emissions. The results showed that all projects were economically viable and had the potential to mitigate up to 36 tons of CO₂eq per year. Additionally, an eco-efficiency indicator (NPV per CO₂eq avoided) was calculated to enable an integrated assessment of economic performance and environmental impact. Projects using 20 m³ biodigesters achieved the best results, with Project 3 being the most eco-efficient (USD256.05/tCO₂eq), while Project 4 yielded the highest absolute return in all economic analysis tools: NPV (USD 9063.81), IRR (25.10%), MIRR (10.95%), PI (USD 1.65), B/C (USD 1.65) and DPP (4.56 years). The integrated analysis underscores the significance of co-digestion and economies of scale in encouraging the adoption of this technology by small rural producers. Full article

Background/Objectives: Healthcare accounts for approximately 4.4% of global carbon emissions. Gastroenterology is a particularly heavy producer, with professional organisations outlining targets to move towards carbon neutrality. Missed hospital appointments, associated with poor medical outcomes, also represent physical and economic waste to the sector. COVID-19 expedited the shift toward virtual clinics, but tele-diagnostics have not expanded similarly. We aimed to assess the feasibility of a virtual C13 urea breath test clinic for H. pylori in Ireland. Methods: C13 urea breath test kits were provided to patients in the community, who were subsequently invited to book an online video appointment with a GI lab technician to assist them in performing the test at home. Completed tests were returned to the hospital via local GP, by post, or a specified hospital drop-off point, and analysed using our standard protocol. Results: 423 virtual appointments were reviewed. 135 (32%) were male, and the mean age was 42 years. The test positivity rate was 22%, similar to a matched in-person testing cohort (21%). In all, there were no non-attenders, and two cancellations. Virtual patients were more likely to attend their appointments (OR = 153.9, p = 0.0004) than in-person patients. Virtual UBT appointments saved 9943.5 Km of road journeys, equivalent to 254 person-hours of travel time and 1.24 metric tonnes of CO₂. Additionally, 300 (71%) patients returned a feedback questionnaire, of which 276 (92%) rated the overall home breath test experience as ‘good’ or ‘excellent’. Conclusions: Home testing for H. pylori is effective, acceptable, and reduces both reliance on invasive procedures such as endoscopy and carbon emissions. Full article

The spray drying of hard metal (WC-Co) powders is a critical step in the production of high-performance cutting and wear-resistant tools. Traditionally, organic solvents such as ethanol or acetone are employed in this process, despite posing substantial health, safety, and environmental risks. This study investigates a sustainable alternative by replacing organic solvents with water in the spray-drying process. We present a comparative analysis of granule morphology, flowability, and final mechanical properties between solvent-based and water-based routes. The water-based approach achieved a d₅₀ of 99 µm, flow time of 27.8 s, and apparent density of 3.18 g/cm³, closely matching the solvent-based values (d₅₀ = 93 µm, flow = 28.4 s, and ρ = 3.14 g/cm³). Hardness (HV30 ≈ 1650) and microstructure were equivalent across both routes, confirming that the substitution does not compromise performance. The water-based process also offers an estimated reduction of over 50% in CO₂ emissions compared to traditional methods. These findings support the feasibility of water-based granulation as a viable, scalable, and safer route for WC-Co powder production, in alignment with dematerialization, circular material use, and the broader goals of sustainable development. Full article

The utilization of carbon dioxide (CO₂) from industrial emissions as an input in microalgal biorefineries represents an integrated strategy that contributes to mitigating and transforming residual resources into value-added products. The valorization of CO₂ from gaseous effluents through biotechnological routes also contributes to the development of a bio-based circular economy. This article aims to present the carbon footprint of a microalgal biorefinery system with CO₂ recovery from exhaust gases for the 193 countries of the world. The results reveal that the tons of carbon dioxide equivalent (tCO₂e) emissions of the proposed biorefinery system can be as low as 3 tCO₂e per year and as high as 590 tCO₂e per year. Countries with emissions greater than 445.98 tCO₂e per year were considered, following a statistical approach, as having low environmental performance in terms of the implementation of the proposed technology. This study’s insights help establish benchmarks for the implementation of microalgal biorefineries that are more capable of recovering industrial emissions—environmentally. Full article

Chlorophylls, which are associated with carotenoids and photosynthetic protein complexes, acquire optical properties that enable the absorption of sunlight, necessary for the synthesis of energy and redox equivalents, necessary for photosynthetic absorption of CO₂ and the production of oxygen as an intermediate product. These processes are important for plants, but also for the biosphere. In stressful situations, when photosynthesis is limited, the production of reactive oxygen and other species increases, and the activation of various protective systems is necessary to remove the aforementioned reactive species or reduce the excessive reduction in photosynthetic electron transport, as the cause of the production of the reactive species. A review of studies where the content and physiological state of chlorophyll are monitored, using destructive and non-destructive methods, such as various optical methods for monitoring its content and physiological activity, is given. Polyphenolic compounds belong to non-enzymatic systems for quenching the reactive species. In addition to their presence in monomeric and oligomeric forms of polyphenols, polymerization of this type of compound can occur. In addition to having a protective effect on the plants that synthesize them, polyphenolic compounds also have a beneficial effect on the health of animals and humans who consume them from plants. Full article