Search Results (95)

Background and Objective: Dysregulated tyrosine kinase signaling is a central driver of tumorigenesis, metastasis, and therapeutic resistance. While tyrosine kinase inhibitors (TKIs) have revolutionized targeted cancer treatment, identifying compounds with optimal bioactivity remains a critical bottleneck. This study presents a robust machine learning framework—leveraging deep artificial neural networks (dANNs), convolutional neural networks (CNNs), and structural molecular fingerprints—to accurately predict TKI bioactivity, ultimately accelerating the preclinical phase of drug development. Methods: A curated dataset of 28,314 small molecules from the ChEMBL database targeting 11 tyrosine kinases was analyzed. Using Morgan fingerprints and physicochemical descriptors (e.g., molecular weight, LogP, hydrogen bonding), ten supervised models, including dANN, SVM, CatBoost, and CNN, were trained and optimized through a randomized hyperparameter search. Model performance was evaluated using F1-score, ROC–AUC, precision–recall curves, and log loss. Results: SVM achieved the highest F1-score (87.9%) and accuracy (85.1%), while dANNs yielded the lowest log loss (0.25096), indicating superior probabilistic reliability. CatBoost excelled in ROC–AUC and precision–recall metrics. The integration of Morgan fingerprints significantly improved bioactivity prediction across all models by enhancing structural feature recognition. Conclusions: This work highlights the transformative role of machine learning—particularly dANNs and SVM—in rational drug discovery. By enabling accurate bioactivity prediction, our model pipeline can effectively reduce experimental burden, optimize compound selection, and support personalized cancer treatment design. The proposed framework advances kinase inhibitor screening pipelines and provides a scalable foundation for translational applications in precision oncology. By enabling early identification of bioactive compounds with favorable pharmacological profiles, the results of this study may support more efficient candidate selection for clinical drug development, particularly in regards to cancer therapy and kinase-associated disorders. Full article

This study underscores how perceived social support, loneliness, and mental well-being shape the emotional bonds that Spanish cat and dog owners form with their pets. A total of 298 participants completed an online survey, including 109 cat owners (36.5%) and 189 dog owners (63.4%). Standardized and validated instruments were used: the Medical Outcomes Study Social Support Survey (MOS-SSS), the UCLA Loneliness Scale, the Warwick–Edinburgh Mental Well-Being Scale (WEMWBS), and the Lexington Attachment to Pets Scale (LAPS). The results confirmed that the attachment to dogs tends to be stronger than to cats, with demographic factors such as gender and age also playing a significant role in the attachment levels. While psychosocial variables did not directly predict the overall attachment scores, lower perceived social support was significantly associated with higher scores on the person substitution subscale among dog owners, indicating that dogs may serve as emotional substitutes for human companionship in socially vulnerable individuals. No comparable association was found among cat owners. These findings highlight species-specific differences in the human–pet bond and emphasize the multifaceted nature of attachment. This study underscores the importance of considering both demographic and psychosocial factors to better understand how pets contribute to human emotional well-being. Full article

This study investigated the effects of taxifolin (Tax), quercetin (Que), (+)-catechin (Cat) and luteolin (Lute) on the advanced Maillard reaction stage in the methylglyoxal-lysine (MGO-Lys) system. Since the four flavonoids share identical A- and B-ring structures, the inhibitory effects and molecular mechanisms of flavonoids with different C-ring structures on N^ε-(carboxyethyl)lysine (CEL) formation were revealed. The results demonstrated that Cat exhibited the best inhibitory effect on CEL with an inhibition rate of 53.78%, while Lute showed the lowest inhibition rate of 3.97%. The flavonoids (i.e., Tax, Que, Cat and Lute) inhibited the formation of non-fluorescent CEL, where hydroxylation at C³ on the C-ring favored the enhancement of the inhibitory effect of the flavonoids on CEL, while the C²-C³ double bond and the carbonyl group at the C⁴ position reduced their inhibitory ability. The alkaline environment favored the enhancement of the inhibition of CEL by Tax, Que, Cat and Lute. Notably, Tax, Que, Cat and Lute can inhibit CEL formation by competitively capturing MGO to form mono- or di-adducts and reducing lysine consumption. This study provides innovative strategies and a theoretical foundation for developing effective CEL inhibitors in food thermal processing. Full article

While pet ownership is sometimes associated with better mental health, research on this relationship has been inconsistent. Some researchers have considered what factors may impact this relationship, such as owners’ attachment to their pets. The aim of this systematic review was to investigate how attachment to pets relates to owner mental health and wellbeing. This review followed PRISMA guidelines. Searches were conducted using PsychInfo, Scopus and Google Scholar. Any peer-reviewed empirical studies, in English, investigating attachment to pets and mental health and wellbeing outcomes, were included. Of the 116 included studies, 15 studies found that higher attachment was associated with better mental health, 22 studies found that higher attachment was associated with worse mental health, 36 studies found mixed results and 33 studies found no significant relationship. Having a stronger attachment to one’s pet tended to be associated with better mental health when investigating outcomes in children, and when investigating outcomes related to wellbeing. Stronger attachment tended to be associated with worse mental health when investigating mental health symptomology (e.g., depression and anxiety). It may also be influenced by the owner’s relationships with other people. Due to the cross-sectional design used by studies in this review, we cannot infer the direction of causality for the relationship between attachment to pets and mental health. Results indicate that having a strong attachment to one’s pet is not necessarily associated with better mental health and wellbeing. Future longitudinal research is needed to better understand how attachment to pets affects mental health, and what factors may influence this relationship. Full article

Traditional methods for managing free-roaming cats in Australia primarily depend on legislation and enforcement to achieve compliance. State laws and local regulations mandate confinement, sterilization, registration, and identification and limit the number of cats kept, with penalties for breaches. However, these strategies fail to address underlying issues like financial constraints in low-income areas and the prevalence of semi-owned cats. Containment mandates often result in increased complaints, shelter intake, and euthanasia, without effectively reducing cat-related problems. Research shows that these approaches are expensive, difficult to enforce, and place a disproportionate burden on disadvantaged communities. Moreover, they negatively affect the mental health of shelter staff and animal management officers, who are frequently exposed to euthanasia and ongoing challenges. An alternative “One Welfare” framework, which recognizes the interconnectedness of animal, human, and environmental welfare, has proven more effective. Programs that provide support and resources, particularly for cat sterilization and microchipping, while fostering the human–animal bond improve outcomes for both cats and caregivers. Shifting from punitive measures to collaborative, community-driven strategies is crucial for managing free-roaming cats in a way that benefits animals, people, and the broader community, while protecting wildlife. Full article

Evidence suggests that autistic children spend less time engaging in social interactions than their neurotypical peers which can negatively impact their wellbeing. Researchers, educators, and parents must consider how we address this. A possible facilitator of autistic children’s social skills and a protective factor for their psychological health is the role of pets and the human–pet bond. The study examined parental reports of autistic children’s attachment to their pet (dog or cat), positive and negative behaviours with that pet, and how this relates to prosocial behaviour, peer problems, and psychological health (emotional difficulties, conduct problems, hyperactivity/inattention). Sixty-five parents with an autistic child completed quantitative measures to assess these variables. Participants also completed qualitative questions aimed at understanding their perceptions of the impact of pets on their child. A regression analysis showed that children’s positive behaviour towards the pet predicted their prosocial behaviour (β = 0.40 p = 0.006). No other regression models were statistically significant. A thematic analysis of the qualitative responses highlighted four themes in relation to parents’ perceptions of the positive impacts of pets on their child. These were (1) Anxiety, Emotion Regulation, and Sleep; (2) Understanding of Self and Other; (3) Communication, Friendships, and Social Interactions; and (4) Comfort and Psychological Health. One theme was identified in relation to the negative impact of pets: (5) Pet-Related Anxiety and Concerns. The findings have implications which can inform guidelines to help parents make decisions about pet ownership and how to foster meaningful relationships between autistic children and their pets. Full article

Hijiki (Hizikia fusiformis) is a seaweed native to warm-temperate and subtropical regions that has a high edible value and economic value, with a production of about 2 × 10⁵ tons/year. Current research has clearly shown that the pharmacological activities of active ingredients from hijiki have covered a broad spectrum of areas, including antioxidant, hypoglycemic, antiviral, anticoagulant, anti-inflammatory, intestinal flora modulation, anti-aging, antineoplastic and antibacterial, and anti-Alzheimer’s disease areas. However, no studies have reported on the production of antioxidant peptides from hijiki proteins. The objectives of this study were to optimize the preparation process and explore the cytoprotective function and mechanisms of antioxidant peptides from hijiki protein. The results indicated that papain is more suitable for hydrolyzing hijiki protein than pepsin, trypsin, alkaline protease, and neutral protease. Under the optimized parameters of an enzyme dosage of 3%, a material–liquid ratio of 1:30, and an enzyme digestion time of 5 h, hijiki hydrolysate with a high radical scavenging activity was generated. Using ultrafiltration and serial chromatographic methods, ten antioxidant oligopeptides were purified from the papain-prepared hydrolysate and identified as DGPD, TIPEE, TYRPG, YTPAP, MPW, YPSKPT, YGALT, YTLLQ, FGYGP, and FGYPA with molecular weights of 402.35, 587.61, 592.64, 547.60, 532.53, 691.77, 523.57, 636.73, 539.58, and 553.60 Da, respectively. Among them, tripeptide MPW could regulate the Keap1/Nrf2 pathway to significantly ameliorate H₂O₂-induced oxidative damage of A549 cells by increasing cell viability and antioxidant enzyme (SOD, CAT, and GSH-Px) activity, decreasing ROS and MDA levels, and reducing the apoptosis rate. Molecular docking experiments show that HFP5 (MPW) exerts its inhibitory effect mainly through hydrogen bonds and hydrophobic interactions with the Kelch domain of the Keap1 protein, eventually facilitating the translocation of Nrf2 to the nucleus. Therefore, antioxidant peptides from hijiki can be applied to develop algae-derived health foods for treating diseases associated with oxidative stress. Full article

The relationship between humans and their pets has long fascinated researchers, particularly in exploring how attachment varies according to the type of pet. Cats and dogs exhibit unique behavioral and social traits that influence the dynamics of human–pet relationships. Moreover, specific human characteristics have been found to affect this attachment. Our study examines the human factors that influence pet attachment among cat and dog owners in the Basque Country, located in northern Spain. By investigating these elements, our research aims to enhance the understanding of how human factors shape the human–animal bond. The study included a total of 202 participants, of whom 66.8% were dog owners, and 74.8% identified as women, with ages ranging from 18 to 74 years. Consistent with many previous studies, our results indicate that attachment is generally stronger with dogs compared to cats and that owner’s characteristics such as being female, younger, not living with children, and the amount of time spent with pets on weekends are linked to stronger attachments to pets. Full article

The health of adult dogs and cats is affected positively or negatively by their development in utero and throughout postnatal growth. Preventive veterinary care is particularly important when animals are physiologically immature and sensitive to modifiable environmental factors. Veterinary nurses/technicians are often at the forefront of promoting healthy development, reinforcing the work of veterinarians and using their knowledge, experience, and passion to lead initiatives with breeders and pet guardians. This opinion article considers the role of qualified veterinary nurses/technicians in the care of puppies and kittens throughout their developmental life stages—gestation, the suckling period, growth post-weaning to puberty or neutering, and late growth to adulthood. Much of their influence is through the education of pet carers; they provide trustworthy information relevant to the individual pet and focused on the practicalities of best husbandry practices. These include practical advice on recognizing dystocia, neonatal care, weaning, introduction to the new home, the prevention and management of infectious diseases, socialization with conspecifics and humans, habituation to potential environmental stresses, nutrition, oral hygiene, and grooming. The veterinary nurse’s goal is not only to see young pets develop into healthy, well-adjusted adults but also to see pet guardians developing sustainable human–animal bonds. Full article

In this study, we explore the substrate-mediated control of self-assembly behavior in diboron molecules (C₁₂H₈B₂O₄, B₂Cat₂) using scanning tunneling microscopy (STM). The structural transformation of B₂Cat₂ molecules from one-dimensional (1D) molecular chains to two-dimensional (2D) molecular arrays was achieved by changing the substrate from Au(111) to bilayer graphene (BLG), highlighting the key role of substrate interactions in determining the assembly structure. Notably, the B-B bond in the molecular arrays on BLG is distinctly pronounced, reflecting a more refined molecular resolution with distinct electronic states than that on Au(111). Density functional theory (DFT) calculations confirm the weak interaction between B₂Cat₂ molecules and the BLG substrate, which facilitates the formation of 2D molecular arrays on BLG. This work demonstrates how controlling substrate properties enables the formation of 1D chains and 2D arrays, providing valuable insights for the design of next-generation molecular electronics and catalysis systems. Full article

This study aimed to investigate differences and similarities of the pet–owner relationship and management of dogs and cats living in the same household. An online survey was completed by 1270 individuals who owned both dog(s) and cat(s). Most of the respondents owned only one dog (56.4%) but two or more cats (63.1%; p < 0.001). Moreover, cats were acquired earlier and were more likely to be neutered than dogs (p < 0.001). The neutering rate was also affected by breed (p < 0.001), age (p < 0.05), and the number of pets owned (p < 0.05). Two types of owners were identified according to how they managed their pets: owners who keep cats both indoors and outdoors and dogs indoors (65.3%) and owners who keep cats indoors and dogs outdoors (34.7%). The former group was characterized by a higher percentage of owners younger than 25 years (p < 0.01), owners with more than one dog or cat, large dogs (p < 0.001), and intact (p < 0.04) and mix-breed cats (p < 0.001). Finally, most respondents described the relationship with their cats (93.0%) and dogs (97.0%) as “loving” or “friendly”. Keeping cats indoors (p < 0.05) and allowing dogs and cats to sleep in the owners’ bedroom were associated with a stronger perceived pet–owner bond. Implications for cat and dog welfare are discussed. Full article

Prion diseases are fatal neurodegenerative disorders caused by the misfolding of the normal cellular prion protein (PrP^C) into its infectious isoform (PrP^Sc). Although prion diseases in humans, sheep, goats, and cattle have been extensively studied, feline spongiform encephalopathy (FSE) remains poorly understood. Genetic factors, particularly polymorphisms in the prion protein gene (PRNP) and prion-like protein gene (PRND), have been linked to prion disease susceptibility in various species. However, no studies have yet investigated the PRND gene in cats with respect to prion diseases. Therefore, we investigated polymorphisms in the feline PRND gene and analyzed their genetic characteristics. We sequenced the coding region of the PRND gene using samples from 210 domestic cats and determined the genotype and allele frequencies of PRND polymorphisms. We identified thirteen novel single nucleotide polymorphisms (SNPs), including six non-synonymous variants and one insertion/deletion (InDel) in the feline PRND gene. Four of the non-synonymous SNPs were predicted to have deleterious effects on the Doppel protein’s structure and function. Notably, the SNP c.97A>G (I33V) showed potential structural clashes, and the others formed additional hydrogen bonds. The LD analysis revealed strong genetic associations between the PRND SNPs and the PRNP InDel, suggesting linkage between these loci in cats. This study identifies novel PRND polymorphisms in domestic cats and provides new insights into the genetic factors underlying feline susceptibility to prion diseases. The strong genetic linkage between PRND and PRNP polymorphisms, coupled with predictions of detrimental effects on Doppel protein structure, suggests that PRND gene variants could influence prion disease progression in cats. These findings provide a foundational framework for future studies on the functional implications of PRND polymorphisms in FSE. To the best of our knowledge, this study is the first report on the genetic characteristics of PRND polymorphisms in cats. Full article

The intimate bonds between humans and their pets create favourable conditions that support the mutual transmission of pathogens in either direction. In this context, veterinarians are essential in informing and educating pet owners about health risks linked to zoonotic pathogens and antimicrobial resistance (AMR). To effectively convey this information, veterinarians should have strong communication skills. To gather insights on pet ownership, veterinary consultations, and risk communication, an online questionnaire was used to survey dog and cat owners in Germany. The survey evaluated the frequency and perception of communication concerning zoonotic pathogens and AMR, deriving a communication score. The findings showed that pet owners rated veterinarian communication with a high average score, reflecting a high satisfaction level. The longer pet owners had been clients, the more frequently they received information on zoonoses and AMR, and the better they rated the communication. However, the results also indicated that the amount of information on zoonoses and/or AMR provided by veterinarians was still lower than desired by pet owners. Risk factors, including pathogen detection, vulnerable individuals, owning imported animals, and feeding them raw meat, fish, offal, or uncooked bones, were regularly present. These findings underscore the critical role of risk communication in preventing zoonoses and AMR. Full article

Aiming at evaluating the bond strength of fiber-reinforced polymer (FRP) rebars in ultra-high-performance concrete (UHPC), boosting machine learning (ML) models have been developed using datasets collected from previous experiments. The considered variables in this study are rebar type and diameter, elastic modulus and tensile strength of rebars, concrete compressive strength and cover, embedment length, and test method. The dataset contains two test methods: pullout tests and beam tests. Four types of rebar, including carbon fiber-reinforced polymer (CFRP), glass fiber-reinforced polymer (GFRP), basalt, and steel rebars, were considered. The boosting ML models applied in this study include AdaBoost, CatBoost, Gradient Boosting, XGBoost, and Hist Gradient Boosting. After hyperparameter tuning, these models demonstrated significant improvements in predictive accuracy, with XGBoost achieving the highest R² score of 0.95 and the lowest Root Mean Square Error (RMSE) of 2.21. Shapley values analysis revealed that tensile strength, elastic modulus, and embedment length are the most critical factors influencing bond strength. The findings offer valuable insights for applying ML models in predicting bond strength in FRP-reinforced UHPC, providing a practical tool for structural engineering. Full article

Dog and cat preference has been associated with a few factors, like owner personality traits, but data regarding other aspects of preference ontogeny and the impact of preferences on pet wellbeing have yet to be examined. In this exploratory study, several of these characteristics, such as exposure to pets when young and as adults and current pet interactions and diet were analyzed from internet survey data. We found that more people identified as dog people (63.3%) versus cat people (36.7%) and preference for dogs remained consistent from childhood to adulthood compared with cats. In individuals who changed species preference, a lack of childhood exposure to cats (47.2%) was significantly associated with the group that changed preferences from dogs to cats from childhood to adulthood, compared with dog ownership as a child in the group that changed preferences from cats to dogs (24.4%). The number of cats and dogs in the home directly correlated with species preference (p < 0.001). Dwelling location was also significantly associated with species preference, with cat people being more likely to live in an urban area and dog people in a rural area (p = 0.002). More time was spent in both active and passive interactions with pets of the preferred species. Cats owned by cat people were more likely to be fed prescription diets compared with cats owned by dog people (p < 0.001). Interestingly, dog people were more likely to feed both their cats (p = 0.012) and dogs (p < 0.001) a raw diet compared with cat people. Additional research is needed to understand the development and impact of owner species preferences on pets to identify risks of suboptimal wellbeing. Full article