Search Results (65)

Adaptive reuse of post-industrial heritage is often studied through technical performance, formal intervention strategies, or decision-support models. While these approaches clarify important aspects of reuse, they give limited attention to how projects evolve through the combined effects of architectural decisions, governance arrangements, financing mechanisms, policy instruments, social programs, and inherited fabric. This paper examines adaptive reuse as a time-structured project trajectory. It applies a hybrid methodology combining within-case reconstruction and comparative cross-case analysis to seven European projects in Brussels, Essen, Rotterdam, San Sebastián, Florence, Vienna, and Barcelona. The cases are analyzed across six dimensions: Asset & Context, Governance & Finance, Circularity, Social & Cultural, Policy & Design, and Outcomes & Transfer. The comparison shows that adaptive capacity depends on the alignment of governance, project time, and intervention strategy. Governance determines who can revise decisions and under what conditions; adaptation time is produced through funding horizons, approval procedures, institutional continuity, and civic or public stewardship; and strategies of retention, replacement, reversible insertion, and incremental occupation distribute future risk differently across project phases. From this synthesis, the paper extracts ten conditional lessons that frame adaptive reuse as configuration knowledge: transferable insights whose relevance depends on the interaction among governance capacity, temporal sequencing, inherited fabric, financing, policy support, and social objectives. The paper argues that knowledge transfer in adaptive reuse should be understood as disciplined translation across comparable constraints, not as the replication of models, rankings, or best-practice templates. Full article

This article revisits the debate on whether the Brussels Agreement and the Ohrid Agreement, including its Implementation Annex, are legally binding. It develops a three-test framework that separates international-law binding force from EU-law legal effects. Tests A and B adapt the International Court of Justice (ICJ) indicators of animus contrahendi and acceptance through subsequent conduct, acquiescence and silence. Test C examines whether the agreements produce legal effects through EU enlargement conditionality, monitoring and reporting. The analysis finds that the treaty status of both instruments remains contestable. The Brussels Agreement is textually specific, yet intent signals are mixed, practice remains reversible and treaty-type obligation structures are weak. The Ohrid Agreement is drafted in a more treaty-like register, but references to a future “legally binding agreement” and the politics of non-signature leave inter se binding force unsettled. Nonetheless, both agreements can produce EU legal effects. They operate as enlargement benchmarks that shape assessments of Serbia’s and Kosovo’s progress, while Commission reporting and standardised compliance indicators may indirectly bind EU institutions through consistency, equal treatment and legitimate expectations. Full article

Cancer therapy is increasingly shaped by the need for agents that are both mechanistically precise and clinically tolerable. Sulforaphane (SFN), a dietary isothiocyanate enriched in cabbage-family vegetables such as cauliflower and Brussels sprouts, has emerged as a pleiotropic modulator of tumor biology. This review synthesizes current evidence that SFN regulates diverse cancer-relevant processes, including redox homeostasis, cell-cycle progression, apoptosis, autophagy and epigenetic remodeling, largely through coordinated effects on transcriptional (for example, Nrf2, MAPK, NF-κB and AP-1), post-transcriptional (microRNAs and messenger RNAs) and epigenetic (DNA methyltransferases and histone deacetylases) networks. We then examine how functional nucleic acids, including aptamers, small interfering RNAs, microRNAs and tetrahedral DNA nanostructures, can be engineered to guide SFN to tumor cells, amplify pathway-specific effects and overcome resistance. Particular emphasis is placed on nanotechnology-enabled delivery platforms that enhance SFN stability, bioavailability and tumor selectivity. Finally, we outline key challenges, such as context-dependent Nrf2 activity, inter-individual variability in metabolism and incomplete clinical validation, and propose priorities for translating SFN-based functional nucleic acid systems into rational, combination-ready strategies for precision oncology. Full article

The Earth’s climate is an open nonlinear system, sustained far from thermodynamic equilibrium by solar radiation and energy and matter exchange among its four major subsystems: atmosphere, ocean, land, and cryosphere. Among these four subsystems, the ocean significantly influences and sustains Earth’s climate over decadal to millennial timescales. Although modern numerical models increasingly capture intricate dynamical details, the fundamental concepts of large-scale ocean variability are less frequently explored. This study revisits ocean circulation through the lens of self-organization theory and synergetics. The key synergetic concepts of mode competition, order parameters, and the slaving principle are interpreted within the framework of general ocean circulation and the Atlantic Meridional Overturning Circulation (AMOC). The Brusselator, a simplified model of a nonlinear dynamical system initially developed in chemical kinetics, serves as a conceptual analog for ocean circulation energy conversion. Despite its high abstraction, this proxy model effectively captures essential bifurcation behaviors, such as Hopf bifurcation transitions and limit-cycle behaviors. This clarifies feedback regulation, instability, and potential regime transitions in the AMOC. The synthesis in this study is intended for an interdisciplinary readership and highlights the broader applicability of synergetic principles to the complex Earth climate system maintained far from equilibrium. Full article

This study investigates the spatial exposure of Brazil’s Legal Amazon (BLA) as the deforestation frontier, operationalized as Brazil’s legally defined Amazon Legal administrative region, in Brazil’s commodity exports to its two largest partners: the European Union (EU) and China. Focusing on agricultural, forestry and mining commodity groups, a destination-specific Relative Concentration Ratio (RCR) and Compound Annual Growth Rate (CAGR) on physical trade data (2002–2024) were used to examine whether contrasting trade governance logics—the regulatory “Brussels Effect” and the scale-driven “Beijing Effect”—are associated with different sourcing geographies from the BLA frontier. We test three competing expectations: EU spatial avoidance, higher Chinese frontier dependence, and compliance-driven consolidation. The results reveal a counterintuitive paradox: despite stricter sustainability governance, the EU displays persistently higher frontier dependence than China in key commodity groups, with RCR trajectories indicating stabilization rather than spatial avoidance. In contrast, China’s frontier dependence declines over time in selected sectors even as import volumes expand substantially, highlighting that changes in frontier exposure cannot be inferred from trade scale alone. CAGR patterns further show strong growth in China-related trade at the national level across commodity groups, alongside sector-specific frontier dynamics within BLA. Overall, the findings provide the strongest support for the consolidation hypothesis: compliance and traceability requirements—public and private—may concentrate EU-linked sourcing among highly auditable, capitalized producers embedded in established frontier zones. These results imply that without explicit spatial targeting, demand-side regulations such as the EUDR may improve product-level assurances yet fail to induce a geographic shift away from deforestation frontiers, potentially reinforcing trade links with established producers in high-risk regions. Full article

The main aim of this study was to evaluate the effect of space-like environment on the chemopreventive activity of Brassica sprouts against thyroid cancer cells in vitro. For this purpose the sprouts of broccoli, kale, kohlrabi, and Brussels sprouts were cultivated in darkness and in microgravity for 5–7 days. Then, the sprouts’ extracts were examined for cytotoxic and antiproliferative activity against thyroid cancer and normal cells. The tested microgravity environment stimulated the cytotoxic activity of kohlrabi sprouts, causing approximately 50% reduction in thyroid cancer cells’ viability, while at the same time increasing the viability of normal thyroid cells. Broccoli sprouts showed the strongest antiproliferative activity against normal thyroid cells, with the best effect visible for darkness conditions, which may contribute to the reduction of thyroid hyperplasia. Microgravity and darkness significantly enhanced the antiproliferative activity of kale, especially in 7-day-old sprouts (inhibition approximately 90%). The tested conditions also increased the antiproliferative activity of kohlrabi sprouts, but in the case of Brussels sprouts the effect was unfavorable. The study showed that microgravity and darkness conditions may have significant influence on the chemopreventive role of Brassica sprouts against thyroid cancer cells in vitro, especially in the case of broccoli and kohlrabi sprouts. Full article

Field measurements of ground vibrations were conducted along the Paris–Brussels high-speed railway (HSR) to systematically analyze vibration characteristics generated by embankment and cutting sections. Utilizing the 2.5D finite element method (FEM), numerical models were developed for both earthworks to evaluate the influences of design parameters on ground vibration responses. Results demonstrate that train axle load dominates vibration amplitude in the near-track zone, while the superposition effect of adjacent wheelsets and bogies becomes predominant at larger distances. Vibration energy attenuates progressively with increasing distance from the track, with medium- and high-frequency components decaying more rapidly than low-frequency components. The dominant vibration frequency is determined by the fundamental train-loading frequency (f₁), which increases with train speed. Distinct attenuation patterns are identified between earthwork types: embankments exhibit a two-stage attenuation process, whereas cuttings undergo three stages, including a vibration rebound phenomenon at the slope crest. Furthermore, greater embankment height or cutting depth reduces ground vibrations, but beyond a critical threshold, further increases yield negligible benefits. A higher elastic modulus of the embankment material correlates with reduced vibrations, and steeper cutting slopes, while ensuring slope stability, contribute to additional mitigation. Full article

Differential equations have demonstrated significant practical effectiveness across diverse fields, including physics, chemistry, biological engineering, computer science, electrical power systems, and security cryptography. This study investigates the dynamics of a Caputo fractional discrete-time modified Brusselator model. Conditions for the existence and local stability of the fixed point $FP$ are established. Using bifurcation theory, the occurrence of both period-doubling and Neimark–-Sacker bifurcations is analyzed, revealing that these bifurcations occur at specific values of the bifurcation parameter. Maximum Lyapunov characteristic exponents are computed to assess system sensitivity. Two-dimensional diagrams are presented to illustrate phase portraits, local stability regions, closed invariant curves, bifurcation types, and Lyapunov exponents, while the 0-1 test confirms the presence of chaos in the model. Finally, MATLAB simulations validate the theoretical results. Full article

Background: The Spanish Interterritorial Council of the National Health System (a central government body) currently recommends vaccination against meningococcal serogroup C (MenC) at 4 and 12 months of age for prevention of invasive meningococcal disease (IMD). The Advisory Committee on Vaccines of the Spanish Association of Pediatrics (a professional medical association) and numerous Spanish regional bodies instead recommend quadrivalent vaccination against serogroups A, C, W, and Y (MenACWY) at 4 and 12 months of age. The central government and Spanish Association of Pediatrics also recommend MenACWY vaccination at 12 years of age. This study assessed the potential public health effects of replacing the MenC vaccination schedule with different MenACWY vaccination schedules in infants. Methods: Here, a static multi-cohort population model was used to evaluate potential effects on public health of IMD due to meningococcal serogroups C/W/Y, comparing MenC infant vaccination (reference strategy) against four different strategies including quadrivalent tetanus toxoid conjugate vaccine (MenACWY-TT; Nimenrix^®, Pfizer Europe MA EEIG, Brussels, Belgium) infant vaccination; all strategies included MenACWY-TT vaccination at 12 years of age. Results: The most effective strategy for infant vaccination was MenACWY-TT at 2, 4, and 12 months, preventing an estimated additional 103 IMD cases, 17 deaths, and 41 cases with long-term sequelae (LTS) versus the reference strategy in the base-case IMD incidence scenario. When strategies included a two-dose infant schedule, the earlier the infant MenACWY-TT vaccine was administered, the more additional cases, deaths, and cases with LTS were prevented (base-case and high-incidence scenarios). Conclusions: This analysis supports implementation of MenACWY-TT as a replacement for MenC vaccination. Full article

Multiple studies have found an association between ambient air quality, noise pollution, green spaces and health. The underlying mechanisms of this association remain partly unknown. In this study, we focus on subjective perception as a potential underlying factor. We assess (I) the association between objective and subjective indicators of the living environment and all-cause mortality, as well as (II) the potential modification of the relationship between objective exposures and all-cause mortality by subjective perception: The data consisted of a linkage between the 2001 census, mortality register data from 1 October 2001 to 31 December 2016 and objective indicators of the residential living environment (air and noise pollution and green spaces). We used Cox regression to investigate the impact of objective and subjective indicators of the living environment and their potential interaction effect on all-cause mortality in the Brussels Capital Region: A negative subjective perception of the residential living environment is associated with an increased risk of mortality, even when controlling for socio-demographic parameters. Similarly, objective indicators of air pollutants and green spaces are also related to mortality. When studying the interaction effect, the beneficial effect of a neutral subjective perception stands out. Subjectively satisfied individuals living in the worst objective conditions showed the highest level of mortality hazard. Noise pollution was the only exception, characterized by the lack of an interaction effect: This study showed that besides objective indicators, the subjective perception of the residential environment also matters, and both interact to influence life chances. Subjective indicators not only have a genuine independent impact but also act as an underlying factor in the relationship between the objective residential environment and health. Full article

Glucosinolates (GSLs) are sulfur-containing compounds predominantly found in cruciferous vegetables such as broccoli, kale, and Brussels sprouts, and are recognized for their health-promoting properties. Upon consumption, GSLs undergo hydrolysis by the enzyme myrosinase, resulting in bioactive compounds like isothiocyanates and specific indole glucosinolate degradation products, such as indole-3-carbinol (I3C) and 3,3′-diindolylmethane (DIM), which contribute to a range of health benefits, including anti-cancer, anti-inflammatory, and cardioprotective effects. This review explores the structure, metabolism, and bioavailability of GSLs. Recent evidence supports the protective role of GSLs in chronic diseases, with mechanisms including the modulation of oxidative stress, inflammation, and detoxification pathways. Furthermore, the innovative strategies to enhance GSL bioactivity, such as biofortification, genetic introgression, and optimized food processing methods, have been examined. These approaches seek to increase GSL content in edible plants, thereby maximizing their health benefits. This comprehensive review provides insights into dietary recommendations, the impact of food preparation, and recent advances in GSL bioavailability enhancement, highlighting the significant potential of these bioactive compounds in promoting human health and preventing chronic diseases. Full article

Transitioning to Positive Energy Districts (PEDs) is essential for achieving carbon neutrality in urban areas by 2050. This study presents a multi-objective optimization framework that balances energy, environmental, and economic performance, addressing the diverse priorities of multiple stakeholders. The framework enhances PED design by systematically evaluating technical solutions, including renewable-based electrification, demand-side management (DSM), energy storage, and retrofitting. The framework is applied to the Usquare district in Brussels, Belgium, as a case study. The results indicate that expanding photovoltaic (PV) capacity is crucial for achieving PED targets, with renewable-based electrification potentially reducing carbon emissions by up to 79%. The incorporation of demand-side management (DSM) and battery storage improves system flexibility, reduces grid dependency, and enhances cost-effectiveness. Although slightly more costly, retrofitting existing buildings provides the most balanced approach, offering the lowest CO2 emissions and the highest self-consumption ratio. This study presents a comprehensive decision-making support framework for optimizing PED design and operation, offering practical guidance for urban energy planning and contributing to global efforts toward carbon neutrality. Full article

Background: Brussels chicory affluent in phenolic acids could inhibit atherosclerosis; however, its effects on exercise performance and post-exercise recovery are unknown. We hypothesized that Brussels chicory could enhance exhaustive aerobic exercise performance and post-exercise recovery by promoting lactate oxidation. Methods: This is a single-blind, randomized, placebo-controlled two-way cross-over trial involving 32 untrained college students (men 18) who consumed either Brussels chicory juice (100 g of Brussels chicory containing ~130 mg phenolic acids and 180 mL fresh milk) or placebo (180 mL fresh milk) for 7 days with a 2-week washout period. On the 7th day, participants received a short-term, progressive workload, high-intensity, exhaustive aerobic exercise with the Bruce protocol. Time to exhaustion and blood lactate were evaluated after exercise. C2C12 myotubes were treated with Brussels chicory phenolic acids (0.625–10 μM) to evaluate these effects on lactate metabolism and lactate dehydrogenase A (LDHA) and B (LDHB), two enzymes responsible for lactate biosynthesis and oxidation, respectively. Results: Brussels chicory consumption increased time to exhaustion by 8.3% and 12.2% for men and women participants, respectively. This administration also promoted post-exercise recovery, evidenced by a reduction in blood lactate (14.5% for men and 10.6% for women). In C2C12 myotubes, Brussels chicory protocatechuic acid and caffeic acid did not affect LHDA-mediated lactate production, whereas these compounds dose-dependently promoted LDHB-mediated lactate oxidation through an enrichment of mitochondria LDHB. Conclusions: Dietary supplementation with Brussels chicory may enhance short-term, progressive workload, high-intensity, exhaustive aerobic exercise performance and post-exercise recovery in humans, possibly by accelerating LDHB-mediated lactate oxidation. Full article

The aim of this study was to compare the concentration of phenolic compounds, glucosinolates, proteins, sugars and vitamin C between kohlrabi (Brassica oleracea var. acephala gongylodes), Savoy cabbage (B. oleracea sabauda), Brussels sprouts (B. oleracea gemmifera), cauliflower (B. oleracea botrytis), radish (Raphanus sativus) and garden cress (Lepidium sativum) microgreens for their antioxidant and hypoglycemic potential. In addition, we applied an in vitro-simulated system of human digestion in order to track the bioaccessibility of the selected phenolic representatives, and the stability of the microgreens’ antioxidant and hypoglycemic potential in terms of α-amylase and α-glucosidase inhibition after each digestion phase. Using spectrophotometric and RP-HPLC methods with statistical analyses, we found that garden cress had the lowest soluble sugar content, while Savoy cabbage and Brussels sprouts had the highest glucosinolate levels (76.21 ± 4.17 mg SinE/g dm and 77.73 ± 3.33 mg SinE/g dm, respectively). Brussels sprouts were the most effective at inhibiting protein glycation (37.98 ± 2.30% inhibition). A very high positive correlation (r = 0.830) between antiglycation potential and conjugated sinapic acid was recorded. For the first time, the antidiabetic potential of microgreens after in vitro digestion was studied. Kohlrabi microgreens best inhibited α-amylase in both initial and intestinal digestion (60.51 ± 3.65% inhibition and 62.96 ± 3.39% inhibition, respectively), and also showed the strongest inhibition of α-glucosidase post-digestion (19.22 ± 0.08% inhibition). Brussels sprouts, cauliflower, and radish had less stable α-glucosidase than α-amylase inhibitors during digestion. Kohlrabi, Savoy cabbage, and garden cress retained inhibition of both enzymes after digestion. Kohlrabi antioxidant capacity remained unchanged after digestion. The greatest variability was seen in the original samples, while the intestinal phase resulted in the most convergence, indicating that digestion reduced differences between the samples. In conclusion, this study highlights the potential of various microgreens as sources of bioactive compounds with antidiabetic and antiglycation properties. Notably, kohlrabi microgreens demonstrated significant enzyme inhibition after digestion, suggesting their promise in managing carbohydrate metabolism and supporting metabolic health. Full article

Petit vert (scientific name: Brassica oleracea var. gemmifera DC. × Brassica oleracea var. acephala DC.) is a new variety of vegetable created by crossbreeding kale and brussel sprouts (Brassica oleracea species). The present study aimed to identify biologically active compounds in extracts of the outer leaves of Petit vert by purification and to examine their biological activities. The dried and powdered outer leaves of Petit vert were extracted, fractionated, and purified to isolate active compounds. Mass spectrometry (MS) was used to identify the compounds, and nuclear magnetic resonance (NMR) spectroscopy was performed to elucidate their structures. The compounds isolated from Petit vert leaves were glycosides that contained kaempferol, quercetin (flavonol), or sinapic acid (phenylpropanoid). Glucose uptake in cultured C2C12 murine myoblasts in the absence of insulin was significantly increased by these compounds, kaempferol, sinapic acid, and ferulic acid, while uptake in the presence of insulin was also significantly increased by compounds 3 and 4, kaempferol, and sinapic acid. The effect was not necessarily concentration-dependent, and some agents decreased the glucose uptake at higher concentrations. The present study reports for the first time the isolation of five compounds containing sinapic acid from the outer leaves of Petit vert and their stimulation of glucose uptake in cultured C2C12 murine myoblasts. The results obtained herein suggest the potential of these compounds to effectively attenuate hyperglycemia and maintain muscle strength by promoting glucose metabolism in muscle cells. Full article