Search Results (102)

Machupo virus (MACV), a member of the Arenaviridae family and causative agent of Bolivian hemorrhagic fever, results in lethality rates of 25–35% in humans. Mice lacking the signal transducer and activator of transcription 1 (STAT-1^−/−) have previously been shown to succumb to MACV infection within 7–8 days via the intraperitoneal route. Despite these reports, we observed partial lethality in STAT-1^−/− mice following challenge with wild-type MACV. Serial sampling studies to evaluate the temporal progression of infection and pathologic changes after challenge revealed a two-phase disease course. The first phase was characterized by viral load and pathological lesions in the spleen, liver, and kidney followed by a second, lethal phase, defined by high viral titers and inflammation in the brain and spinal cord resulting in neurological manifestations and subsequent mortality. Tissue adaptation in the brains of challenged STAT-1^−/− mice resulted in a fully lethal model in STAT-1^−/− mice (mouse-adapted; maMACV). A similar two-phase disease course was observed following maMACV challenge, but more rapid dissemination of the virus to the brain and overall pathology in this region was observed. The outcome of these studies is a lethal small rodent model of MACV that recapitulates many aspects of human disease. Full article

Quinoa (Chenopodium quinoa) cultivation and consumption have been increasing globally for its nutritional value and agricultural adaptability, with over 120 countries involved in its production. In Canada, quinoa is cultivated as a specialty crop to increase crop diversity and support agroresilience. This study is the first to examine quinoa cultivars grown under northern Quebec conditions and to provide a nutritional and sensory characterization of two Quebec (Canada) varieties (Sweet and Bitter) in comparison to the Bolivian reference cultivar, Royal White. Analyses included proximate composition, amino acids, fatty acids, phenolics, and anti-nutrients. Sensory evaluations involved hedonic and bitterness ranking tests. Bolivian cultivar had higher omega-3 content, while the Quebec cultivars showed favorable protein and lipid profiles, with better lipid health indexes. Protein quality was comparable between the Bolivian and Sweet cultivars. The overall flavor appreciation was similar among twice-brushed Bitter cultivar and Bolivian samples. The Bolivian sample received a better score for texture. Descriptive flavor data support the development of a quinoa flavor lexicon. Notably, total saponins content, commonly used as a bitterness indicator, did not consistently correlate with perceived bitterness, emphasizing the need for a standardized quantification method for cultivar selection and further investigation into other flavor-contributing compounds. Full article

This retrospective cohort study aimed to assess clinical and epidemiological characteristics, treatment outcomes, and predictors of serological cure in patients with chronic Chagas disease in a non-endemic setting. All individuals aged ≥16 years with confirmed infection and evaluated at a tertiary hospital in Spain from 2008 to 2023 were included. Most of the 107 participants were women (78.5%) and Bolivian-born (99.1%). Digestive and cardiac involvement were identified in 32.7% and 17.8% of cases, respectively. Cardiac symptoms were significantly associated with the diagnostic findings of cardiac involvement (odds ratio [OR] 3.0, 95% confidence interval [CI] 1.1–8.2), whereas digestive symptoms did not correlate with imaging abnormalities (OR 0.7, 95% CI 0.3–1.6). Antiparasitic treatment, usually benznidazole, was initiated in 69% of patients and led to adverse events in 66.2%, with treatment discontinuation in 25.7%. Only 8.1% of treated patients achieved serological cure after a median 26 months, with obesity emerging as the only independent predictor (adjusted OR 31.0, 95% CI 3.7–261.2). Cardiac progression occurred in 9.3% of patients despite treatment. Although 59.8% were lost to follow-up, the cohort maintained a median follow-up of 27 months. These findings underscore the need for improved treatment strategies and sustained clinical monitoring in non-endemic settings. Full article

Amaryllidaceae alkaloids from the Amaryllidoideae subfamily exhibit broad pharmacological activities, including neuroprotection and anticancer effects. Galanthamine is a key compound for Alzheimer’s therapy. The Hippeastrum genus, particularly in Bolivia, offers significant potential for novel drug discovery, emphasising the need for conservation and further phytochemical research. Twenty-seven samples from Bolivian Hippeastrum species were investigated in terms of their alkaloid profile and anticholinesterase activity. The phytochemical analysis of Bolivian Hippeastrum species via GC-MS identified 48 Amaryllidaceae alkaloids, displaying diverse structural groups with potential pharmacological significance. Lycorine- and Homolycorine-type alkaloids were predominant, particularly in H. chionedyanthum and H. haywardii, with high concentrations of lycorine, a promising anticancer compound. The species H. evansiarum and H. mollevillquense contained notable quantities of Galanthamine type alkaloids, relevant for Alzheimer’s treatment. This study also highlights variability in acetylcholinesterase and butyrylcholinesterase inhibitory activities, with H. lara-ricoi and H. haywardii demonstrating strong inhibition. These findings suggest that Hippeastrum species are a valuable source of bioactive compounds, warranting further research into their therapeutic applications. Full article

Background/Objectives: The objective of this research was to analyze the experiences and factors associated with perinatal grief in mothers in the urban context of Santa Cruz de la Sierra, Bolivia. Methods: The sample consisted of seven mothers who experienced a loss during pregnancy up to the second month after the baby’s birth, occurring between 2015 and 2020 in the city of Santa Cruz de la Sierra. The mean age of the mothers was 34.86 years (SD = 3.13), and they belonged to middle or upper-middle socioeconomic levels. Data were collected through semi-structured interviews and analyzed using descriptive qualitative analysis. Results: The identified characteristics of perinatal grief were sadness, anger, guilt, emotional numbness, social isolation, and anxiety. Factors contributing to grief processing included support from the partner and family, when they accommodated and respected the mother’s needs. Factors hindering the grieving process included social and cultural environments that often silence and minimize the loss, a history of previous losses, the desire to be pregnant, and the mother’s life expectations and projects focused on motherhood. Conclusions: In conclusion, this research suggests that perinatal losses in the Bolivian context may be influenced by factors such as knowledge of the cause of death, previous loss experiences, and their emotional effects. The limitations of the study include the lack of diversity in participants’ educational and socioeconomic backgrounds and the restriction of the sample to an urban area in Bolivia. Emotional interventions to support these bereaved mothers in those complex moments should be integrated in the Bolivian healthcare system. Full article

Medicinal plants are widely used in Bolivian folk medicine for the treatment of infectious diseases. We have selected one, Clinopodium bolivianum (Benth.) Kuntze, known as “Khoa”, to investigate its potential anti-HIV activity since, traditionally, it has been used to treat other viral infectious diseases. We have carried out an antiviral bioassay-guided fractionation of different extracts of the aerial parts of C. bolivianum. An antiviral crude polysaccharide was obtained, (SBA_S), which is rich in glucose, galactose, mannose, arabinose, xylose, and rhamnose and only has traces of galacturonic acid. SBA_S exhibited antiviral activity with a mechanism of action unrelated to the mannose–lectin DC-SIGN receptors but with a strong viral neutralization activity. In summary, a purified polysaccharide from C. bolivianum has been identified as the main compound responsible for its antiviral activity. SBAs proved to be a neutralizing agent with high antiviral capacity in vitro, so they could be part of new microbicide formulations to prevent HIV transmission. Full article

Urinary tract infections (UTIs) are common and are typically treated empirically, based on local antimicrobial resistance (AMR) data, which are often scarce in low- and middle-income countries. This study examines the AMR patterns of pathogens causing community-onset (CO) UTIs in the Bolivian Chaco. Urine samples were collected from subjects with suspected CO-UTIs and analyzed by culture techniques. Significant isolates were tested for their antimicrobial susceptibility. Additionally, bla_CTX-M and mcr genes were searched for using real-time PCR. A total of 361 CO-UTI episodes were diagnosed among 731 subjects from February 2020 to November 2021. The cases included uncomplicated and complicated UTIs (58.2% and 41.8%, respectively), with females accounting for the majority (85.3%) of cases. Escherichia coli was the most prevalent pathogen (86.6%), followed by Klebsiella pneumoniae (5.4%) and Proteus spp. (2.2%). Very high resistance rates (>50%) were observed for ampicillin, trimethoprim–sulfamethoxazole and fluoroquinolones, high resistance rates (>20%) for amoxicillin–clavulanate, third-generation cephalosporins and gentamicin, while lower resistance rates (<10%) were observed for nitrofurantoin and fosfomycin. The prevalence of bla_CTX-M among E. coli was high (26.7%). Colistin resistance was detected in 3.4% of E. coli, mostly associated with mcr genes. CO-UTIs from this area were characterized by high resistance rates to commonly used antibiotics (trimethoprim–sulfamethoxazole, amoxicillin–clavulanic acid and ciprofloxacin), highlighting the importance of knowledge of the local epidemiology to inform the selection of appropriate empirical antibiotic regimens. Full article

This paper explores the intersection of race, religion, and colonial legacies through the lens of the Oruro Carnival, examining its role in shaping Bolivian identity. Critical religion scholars argue that the entanglement of race and religion is a product of Western modernity and colonialism, which has influenced both historical and contemporary power relations. This framework is applied to analyse the Carnival, where religious practices and festive performances intersect, reflecting colonial efforts at religious conversion and racial categorisation. By focusing on the ethnography of Oruro’s embodied festive practices, this study investigates how the Carnival contributes to the construction of difference amid Bolivia’s socio-political transformations. This paper also examines how, by the 20th century, colonial religious frameworks intertwined with secular racial categories, particularly through the rise of mestizaje as a nation-building discourse. A historical analysis of Carnival performances reveals how race, religion, and power have continually shaped the celebration, tracing its evolution from a segregated religious practice to a national spectacle, particularly after the 1952 revolution. The mutually configuring relationship between race and religion in Carnival highlights its role in both reinforcing and challenging dominant power structures. Full article

Quinoa (Chenopodium quinoa Willd.), as one of the quasi-cereal crop plants with high nutritional value and yield potential, especially in stressful environments, has recently been proposed as a suitable alternative plant for sustainable nutrition of the world’s growing population. In Iran, this plant has been considered as a valuable crop for several years, but since quinoa is native to the South American region, therefore, while assessing the compatibility of different imported cultivars, it is necessary to introduce stable high-yielding cultivars for different regions of the country. The objective of the current study was to investigate the GEI and the adaptability and stability of grain yield of 20 Bolivian and Peruvian quinoa genotypes. The experiment layout was a randomized complete block design with three replications in Kuhdasht and Poldokhtar counties, Lorestan province, Iran, during two cropping years, 2020 and 2021. To evaluate the stability of genotypes, the methods of Roemer’s environmental variance, Francis and Kannenberg’s coefficient of variation, Shukla’s stability variance, Wricke’s equivalence, the regression coefficient of Finlay and Wilkinson, the deviation from regression line of Eberhart and Russell, the intra-location variance of Lin and Binns and the GGE-Biplot were used. The results of combined analysis of variance showed a significant difference between genotypes and environments as well as the genotype × environment interaction at 1% probability level. The results of stability analysis of the genotypes using different methods were also very different, but in total, using all studied stability criteria along with grain yield, four genotypes 7, 10, 14, and 15 were identified as the most stable and productive genotypes. In addition to low-yield fluctuations and mean grain yield of more than 3000 kg.ha⁻¹, these genotypes had other suitable characteristics such as dwarfism, early maturity and low saponin content, and are introduced as the superior genotypes of this experiment for cultivation in the studied areas. Full article

This article describes a new genus and species of cyanobacteria isolated from Laguna Pastos Grandes in the Bolivian Altiplano. This discovery marks the first described species of this phylum from this extreme environment. Additionally, the phylogenetic status of the genus Capilliphycus is reassessed. The analyses are based on 16S rRNA gene maximum likelihood and Bayesian phylogenies, identity (p-distance), morphology and habitat comparisons. The new genus is a Lyngbya-like type from a mat at the margin of a brackish–alkaline lake with borax. It is phylogenetically close to Dapis, Tenebriella and Okeania, but compared to these genera, the maximum 16S rRNA gene identity values reached only 90.9%, 91.1% and 92.5%, respectively. The genus Capilliphycus was found to be polyphyletic. The type species Capilliphycus salinus is clustered with C. guerandensis. However, “C. tropicalis” and “C. flaviceps” form a distinct clade, distant from the Capilliphycus type species. Between the two “Capilliphycus” clades, Sirenicapillaria and Limnoraphis are found. Full article

This study investigates the medicinal potential of cultivated plants and weeds in Aymara communities around Lake Titicaca. It highlights the intricate connection between horticultural diversity and traditional healing practices. Through ethnobotanical research involving 228 informants across multiple locations in Bolivia, we documented 239 medicinal plant species, focusing on the diversity within cultivated landscapes. Among these, 56 species are cultivated crops, 17 are agricultural weeds, and 19 species have dual status, serving as both wild and cultivated plants depending on environmental conditions. Women are repositories of knowledge for 81% (193) of total medicinal plant species, while men know 47% (113) of species. Women display dominant knowledge of cultivated species (89%, or 50 species) and purchase medicinal plants from local markets; women know 92% (24 species) vs. men’s 15% (4 species). Our results suggest men may use a smaller set of species more frequently, while women know and use a broader range of species. The analysis of plant life forms revealed the significant medicinal roles of perennials, annuals, subshrubs, and shrubs, which together account for over 67% of the documented species and 73% of the use reports. Arboreal species are present but have a comparatively smaller role in traditional medicine. In total, we recorded 1477 use reports addressing 260 pathologies across 28 major health categories. Notably, cultivated plants and weeds represent 38.8% of the medicinal species, highlighting their essential role in local healthcare practices. By assessing the contributions of native and introduced species, this study sheds light on the complex botanical resources integrated into Aymara agricultural systems. These findings deepen our understanding of medicinal plant diversity and underscore the importance of agrobiodiversity as a cornerstone of community health and cultural resilience in the Andean region. This research also emphasizes the often-overlooked medicinal value of cultivated landscapes and agricultural margins. Full article

Climate prediction is of fundamental importance to various sectors of society and the economy, as it can predict the likelihood of droughts or excessive rainfall in vulnerable regions. Climate models are useful tools in producing reliable climate forecasts, which have become increasingly vital due to the rising impacts of climate change. As global temperatures rise, changes in precipitation patterns are expected, increasing the importance of reliable seasonal forecasts to support planning and adaptation efforts. In this study, we evaluated the performance of NOAA/GFDL models from CMIP6 simulations in representing the climate of South America under three configurations: atmosphere-only, coupled ocean-atmosphere, and Earth system. Our analysis revealed that all three configurations successfully captured key climatic features, such as the South Atlantic Convergence Zone (SACZ), the Bolivian High, and the Intertropical Convergence Zone (ITCZ). However, coupled models exhibited larger errors and lower correlation (below 0.6), particularly over the ocean and the South American Monsoon System, which indicates a poor representation of precipitation compared with atmospheric models. The coupled models also overestimated upward motion linked to the southern Hadley cell during austral summer and underestimated it during winter, whereas the atmosphere-only models more accurately simulated the Walker circulation, showing stronger vertical motion around the Amazon. In contrast, the coupled models simulated stronger upward motion over Northeast Brazil, which is inconsistent with reanalysis data. Moreover, we provided insights into how model biases may evolve under climate change scenarios. Future climate projections for the mid-century period (2030–2060) under the SSP2-4.5 and SSP5-8.5 scenarios indicate significant changes in the global energy balance, with an increase of up to 0.9 W/m². Additionally, the projections reveal significant warming and drying in most of the continent, particularly during the austral spring, accompanied by increases in sensible heat flux and decreases in latent heat flux. These findings highlight the risk of severe and prolonged droughts in some regions and intensified rainfall in others. By identifying and quantifying the biases inherent in climate models, this study provides insights to enhance seasonal forecasts in South America, ultimately supporting strategic planning, impact assessments, and adaptation strategies in vulnerable regions. Full article

Quinoa (Chenopodium quinoa Willd.) is a new, promising non-conventional useful crop; however, its susceptibility to downy mildew, caused by Peronospora variabilis, is a key obstacle limiting its productivity in Egypt. Identifying and utilizing resistant quinoa cultivars appear to be reliable and cost-efficient strategies for controlling downy mildew, particularly in resource-limited farmers’ fields. This study aimed to evaluate the differential resistance of the Peruvian “Hualhuas” and Bolivian “Real” quinoa cultivars to P. variabilis infection under laboratory conditions to provide precise insight into their basic defense mechanism(s). Inoculated “Hualhuas” plants displayed complete resistance against P. variabilis, with no visible symptoms (incompatible reaction), while those of “Real” plants revealed high susceptibility (compatible reaction), with typical downy mildew lesions on their leaf surfaces. Disease incidence reached about 66% in the inoculated “Real” plants, with most inoculated leaves having lesions of grades 4 and 5 covering up to 90% of their leaf surfaces. Susceptibility indices reached up to 66% in the inoculated “Real” plants. Resistance to P. variabilis observed in the “Hualhuas” plants may have been largely attributed to elevated endogenous H₂O₂ levels, increased peroxidase (POX) activity and abundance, enhanced phenylalanine ammonia-lyase (PAL) activity and expression, as well as the upregulation of the pathogen-related protein 10 gene (PR-10). The results of this study indicate that the quinoa cultivar “Hualhuas” not only is a promising candidate for sustainable control of quinoa downy mildew but also, through a deep understanding of its molecular resistance mechanisms, would provide a possible route to enhance downy mildew resistance in other genotypes. Full article

Heritage encompasses features integral to the culture of a particular society, such as traditional foods that have historical significance and continue to be part of the community’s identity. These culinary traditions must be preserved to sustain cultural identity and community well-being. This study aims to document the role of qarasiña, along with other ancestral foods, in establishing quinoa (Chenopodium quinoa) as a cultural keystone species in the Jach’a Puni community. Primary data were gathered through in-depth interviews with local experts and community observations, supplemented by a comprehensive literature review. Findings reveal that in Jach’a Puni, all food is considered sacred. Three main types of qarasiña are identified: jupha qarasiña, ajara qarasiña, and kita qarasiña. The primary ingredients are domesticated quinoa (jupha) flour (Chenopodium quinoa var. quinoa) and wild quinoa (ajara) flour (Chenopodium quinoa var. melanospermum and C. hircinum). Qarasiña is integral to community banquets and social events. Although quinoa is celebrated globally as the “golden grain”, there is a risk that qarasiña may disappear and quinoa could be replaced by wheat or maize, threatening its status as a cornerstone species in the Bolivian Altiplano. Full article

This paper aims to explore how four Bolivian small and medium-sized enterprises’ business has overcome the gaps in reliance on traditional small and medium-sized enterprises’ business models, i.e., to extract and sell raw unrefined natural resources in a local area, and instead make productive use of innovation inputs (technology, higher-educated people) by innovating their business models. We were particularly interested in how the small and medium-sized enterprises could manage to develop their business models in relation to the socio-cultural, economic, and technological contexts in a lower middle-income country such as Bolivia. We employ an exploratory multiple case study. The study’s results show that the four selected small and medium-sized enterprises’ business model innovation processes followed two different business model innovation patterns, a technology-driven pattern and market-driven pattern shaped by the macro-level factors of availability of natural resources, the informally organized economy, regulations, and access to higher education resources. The paper ends with presenting the managerial, policy, and theoretical implications of the study. Full article