Search Results (22)

Nabatean thyme (Thymbra nabateorum (Danin & Hedge) Bräuchler) is a perennial herb species under the Lamiaceae family, first described in 1998. The species is thought to be endemic to Jordan with only six known records. However, recent fieldwork has uncovered new patches of Nabatean thyme in northwestern Saudi Arabia. This study aimed to determine the global conservation status and update the distribution of Nabatean thyme. To achieve this, we conducted extensive fieldwork and used the collected occurrences to calculate species Extent of Occurrence (EOO) and Area of Occupancy (AOO). We recorded Nabatean thyme in sandstone grooves within open plains at altitudes of 850 to 1350 m, with its largest population occurring outside its historical range, rendering it endemic to northwest Arabia. The primary threats to Nabatean thyme across its range include overgrazing, excessive harvesting for medicinal purposes, and habitat fragmentation. Nabatean thyme has an EOO of 47,585 km² and an AOO of 136 km². Accordingly, we recommend considering Nabatean thyme as an Endangered species under the B2ab(iii) and C2a(ii) IUCN criteria. We recommend integrating both in situ and ex situ conservation programs to improve the conservation status and ensure the sustainability of Nabatean thyme. Full article

The impoundment of Aoos springs was created in 1990 to facilitate hydroelectric production, and fish fauna has been enriched through the years due to deliberate or accidental fish stockings, with certain invasive alien fish species arising (i.e., Lepomis gibbosus, Carassius gibelio), some of which are ranked among the most dangerous species for global biodiversity. A three-year monitoring survey was conducting to map the nesting areas of Pumpkinseed, L. gibbosus, to assess the effectiveness of electrofishing as an active method to reduce the spread of the corresponding species and to identify the impact of the L. gibbosus on native species. The largest percentage of nests was found in areas with silty-sand bottoms (53.4%) and low vegetation. The potential nesting area was estimated at 1.97 km² and the area of confirmed nesting was 0.33 km². The species appeared to nest in a small area, compared to the potential nesting area. The results also indicate that a significant percentage of the target species (71.6% of the total number of species) was removed, with an average time per sampling of 1.2 ± 1 h, whereas the impact on the other native species was minimal. These results are important for designing and implementing programs for the eradication or reduction of L. gibbosus in the impoundment of Aoos springs. Full article

Effects of pulsed electromagnetic fields (PEMF) on immunological factors in a 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) model were investigated. Hairless mice were randomly assigned to control, acetone and olive oil solution-treated (AOO), PEMF 15 Hz, PEMF 75 Hz, and sham groups (n = 5 each). AOO solution was used to dissolve DNCB. Both PEMF and sham groups were exposed to similar DNCB doses, causing similar AD symptoms. After AD induction for five weeks, only the PEMF groups were exposed to PEMF stimulations (15 Hz, 75 Hz, and 15 mT) inside the solenoid coil, for two weeks. In both groups, splenomegaly was observed, as AD was induced by hyperimmune reactions caused by DNCB sensitization. However, splenomegaly did not occur in the PEMF-exposed groups, and spleen weight decreased similarly to that of the control. Hence, the total splenocytes in the PEMF group were similar to those in the control group, whereas the sham group showed three times the number of splenocytes compared with the PEMF group. The serum immunoglobulin E levels did not significantly change in the PEMF group; however, they increased more than fourfold in the sham group. These results demonstrate that PEMF stimulation ameliorated the abnormal symptoms caused by hyperimmune reactions. Full article

Leucine-rich repeat kinase 2 (LRRK2) c.6055G>A (p.G2019S) is a frequent cause of Parkinson’s disease (PD), accounting for >30% of Tunisian Arab-Berber patients. LRRK2 is widely expressed in the immune system and its kinase activity confers a survival advantage against infection in animal models. Here, we assess haplotype variability in cis and in trans of the LRRK2 c.6055G>A mutation, define the age of the pathogenic allele, explore its relationship to the age of disease onset (AOO), and provide evidence for its positive selection. Full article

The bridge of Konitsa over the Aoos river, in Epirus, Greece, was built in 1869. It is one of the most important stone arched bridges in the Balkans, listed by the Hellenic Ministry of Culture (1982). The bridge, damaged by the Ottoman Army in 1913, was restored the same year by French engineers using reinforced concrete. Structural deterioration, located mainly in the area damaged by the explosion and subsequently repaired, led to the study of the bridge by the National Technical University of Athens (NTUA), in the framework of a Contract among the Prefecture of Epirus, the Municipality of Konitsa (owner of the bridge), the Ministry of Culture and NTUA. The entire study includes the exhaustive documentation of the bridge, its numerical modelling and assessment at its current state, the selection of adequate interventions and the numerical investigation of the efficiency of the proposed interventions. During this process, one of the main issues was the treatment of the concrete intervention of 1913. For the choice and for the design of the restoration measures, a calculation methodology was adopted, based on the findings of the documentation of the bridge, while taking into account the critical phases (construction, damage and repair) over its lifetime. This work has proven the available safety of the bridge under its self-weight, as well as the need for reconstruction of the RC jacket at the intrados of the arch, which was applied as a repair measure to the bridge in 1913. Full article

The ecosystem structure of a Mediterranean high-altitude artificial lake (Aoos Springs, Northwest Greece) was assessed and quantified using the Ecopath with Ecosim model. The artificial lake was created in 1990 for hydroelectric production and the fish fauna has been enriched across years due to deliberate or accidental fish stockings, as well as the introduction of the invasive pumpkinseed (Lepomis gibbosus), which may cause adverse effects on aquatic ecosystems and their biota. The model considered 20 species/taxa groups with biological data gathered during 2021–2022 from field surveys, interviews with recreational anglers and literature. The results revealed that the artificial lake of Aoos is a resilient ecosystem to unexpected events with low intensity of fisheries exploitation. The biomass of the introduced invasive species Lepomis gibbosus is low, due to the low biomass level of its diet, Chironomidae and zoobenthic organisms as well as other fish species. Full article

This study focused on assessing the impact of climate change on Cupressus funebris, one of the important endemic conifer species in China. The impact of fluctuations in temperature on the potential distribution and extinction risk of the C. funebris was evaluated using species distribution modelling. The outcomes of this current study revealed that the species was mainly distributed in mountainous forest areas, and climate variables played a major role in the distribution of this conifer tree. Under climate change, the threatened conifer will be mainly concentrated in mountainous forests and is projected to undergo contraction in distribution and shift northward. The conservation status of C. funebris is projected to be uplisted to “Near Threatened” status under the SSP5_8.5 scenario by 2040 and to the “Least Concern” status under all the other investigated climate and dispersal scenarios. Despite the high capacity of the species for adaptation to environmental changes, part of its AOO might be lost under severe climate change conditions. Key conservation areas were identified, and suggestions for redesigning some of the current natural reserves in the forested area where the species is found were proposed for the conservation of this key coniferous species. The stable area in the northwestern part of Yunnan in the Hengduan Mountain Forest can serve as a promising area for C. funebris reintroduction through afforestation programs. Full article

In Alzheimer’s disease (AD), the age of onset (AoO) exhibits considerable variability, spanning from 40 to 90 years. Specifically, individuals diagnosed with AD and exhibiting symptoms prior to the age of 65 are typically classified as early onset (EOAD) cases. Notably, the apolipoprotein E (APOE) ε4 allele represents the most extensively studied genetic risk factor associated with AD. We clinically characterized and genotyped the APOEε4 allele from 101 individuals with a diagnosis of EOAD, and 69 of them were affected carriers of the autosomal dominant fully penetrant PSEN1 variant c.1292C>A (rs63750083, A431E) (PSEN1+ group), while there were 32 patients in which the genetic cause was unknown (PSEN1− group). We found a correlation between the AoO and the APOEε4 allele; patients carrying at least one APOEε4 allele showed delays, in AoO in patients in the PSEN1+ and PSEN1− groups, of 3.9 (p = 0.001) and 8.6 years (p = 0.012), respectively. The PSEN1+ group presented higher frequencies of gait disorders compared to PSEN1− group, and apraxia was more frequent with PSEN1+/APOE4+ than in the rest of the subgroup. This study shows what appears to be an inverse effect of APOEε4 in EOAD patients, as it delays AoO and modifies clinical manifestations. Full article

This paper investigates the potential impacts of climate change and anthropogenic activities on the transboundary river of Aoos/Vjosa, flowing through Greece and Albania, and proposes strategies for its protection. The paper analyzes the current state of the river and its ecosystems, identifies the key drivers of change, and examines the potential impacts of future climate scenarios and human activities. Using climate data and GIS mapping, the study highlights the importance of sustainable development and cross-border cooperation to ensure the long-term health and sustainability of the river and its resources. Full article

In the present study, the biology of the pumpkinseed Lepomis gibbosus in the artificial lake of Aoos, located in northwestern Greece, was investigated. The samplings of the pumpkinseed were conducted from the shore using a portable electrofishing device over a 4-month period (July 2021–October 2021). A total of 581 specimens were caught, with an average length of 62 mm. The sex ratio of female to male was estimated to be 1.0:1.7, and the percentage of mature specimens was estimated for all of the months to be above 52%, matching the highest percentage in July (57.4%). The b value of the length–weight relationship ranged from 3.16 in September to 3.31 in July. The value of the L∞ and K was estimated to be equal to 119 mm and 0.36 years⁻¹, respectively, and the value of φ′ was equal to 3.707. The total mortality was estimated to be equal to 1.63 ± 0.48 y⁻¹ (R² = 0.96), and the natural and fishing mortalities were 0.83 and 0.80, respectively. The maximum age was 6 years, and the theoretical maximum age was 8 years. In the current study, the value of the L∞ was estimated to be near the European average but significantly lower than the North American one, whereas the value of the K was slightly higher than the European average. The small size of the specimens obtained in Aoos Springs was most likely owed to the combined impact of the investigated lake’s high altitude and low food availability, resulting in a limited factor for species expansion. Full article

In this study, we used genetic approaches to assess the conservation status of a protected amphibian species, the Macedonian crested newt, Triturus macedonicus, in Northern Pindos National Park (Epirus, Greece). Mitochondrial DNA sequences and multilocus genotypes of individuals from 38 breeding sites were used to infer their phylogenetic position and to detect and measure genetic variation patterns, population genetic structure, and levels of gene flow. The examined individuals fell within two major clades of the Macedonian crested newt phylogeny, being geographically separated by the Aoos River valley and Vikos Gorge. Both groups constitute separate gene pools, bearing private haplotypes and alleles, and the groups were found to be highly differentiated in both their mitochondrial and microsatellite markers. Thus, they meet all of the criteria needed to be characterized as evolutionary significant units (ESUs) that deserve a separate conservation status. Within each ESU, the particularly high inter-population differentiation and low migration rates imply a lack of connectivity between breeding sites and local isolation in favorable habitats. Our results can inform future programs and actions towards the conservation and management of the Macedonian crested newt in the area. Full article

Hydrodiplomacy is the emerging framework where legal acts, based on technical data and information, aim to support commonly accepted solutions to water-related tensions among states with transboundary waters. In this research, hydrodiplomacy components in relation to (a) policy, (b) preventive, (c) cooperative, and (d) technical aspects are considered together with climate change, which is bound to destabilize the core element of hydrodiplomacy, i.e., water. The study area is composed of the five transboundary river basins of Greece. The coupling of all these different nature elements is conducted with the use of the AHP multicriteria method, and the results of a normalized output that quantifies water transboundary cooperation in the climate crisis era are given. Full article

The westernmost European nucleus of the 5220* Habitat of Community Interest (HCI) is located in the province of Málaga (Andalusia). In this area, the 5220* HCI is characterized by the presence of scrublands of Gymnosporia senegalensis subsp. europaea. This is a relict species in Europe, with inhabits only in the southeast of the Iberian Peninsula. The westernmost Iberian nuclei of the 5220* HCI are constituted by three isolated nuclei (Málaga–Rincón de la Victoria; Torremolinos; and Pizarra). These nuclei have been only partially mapped. The objectives were: to map the 5220* HCI characterized by G. senegalensis subsp. europaea in detail; to evaluate its degree of conservation (DC); and to identify the chronosequences of the evolution of this habitat from 1957 to 2021, and its fragmentation. Our results have contributed to generating a 1:10,000 scale cartography of the habitat. In general, the DC obtained was from good to excellent. With an excellent DC value, one inland locality (Pizarra) was highlighted. However, the highest reduction in the value of DC was observed in the localities of Torremolinos and Málaga–Rincón de la Victoria which, in addition, have reduced the area of occupancy (AOO) and are fragmented. It is important to note that some areas of Málaga–Rincón de la Victoria reached excellent values of DC, indicating the need to carry out protection. Full article

Irreversible electroporation (IRE) ablation is a novel treatment option for localized prostate cancer. Here, we present a case of an abrupt and fatal arrhythmia during the IRE procedure in a prostate cancer patient with an implanted permanent pacemaker. A 78-year-old male patient with a pacemaker due to sick sinus syndrome and syncope was scheduled for IRE prostate ablation surgery under general anesthesia. He had a history of recovering from coronavirus disease 2019 (COVID-19) after having been vaccinated against it and recovered without sequalae. Pacemaker interrogation and reprogramming to asynchronous AOO mode was carried out before surgery, however, sinus pause occurred repeatedly during ablation pulse delivery. After the first sinus pause of 2.25 s there was a decrease in continuous arterial blood pressure (ABP). During the delivery of the second and third pulses, identical sinus pauses were observed due to failure to capture. However, the atrial-paced rhythm recovered instantly, and vital signs became acceptable. Although sinus pause recovered gradually, the duration thereof was increased by the delivery of more IRE pulses, with a subsequent abrupt decrease seen in blood pressure. The pacemaker was urgently reprogrammed to DOO mode, after which there were no further pacing failures and no hemodynamic adverse events. For patients with pacemakers, close cardiac monitoring in addition to the interrogation of the pacemaker during the electromagnetic interference (EMI) procedure is recommended, especially in the case of having a disease that may aggravate cardiac vulnerability, such as COVID-19. Full article