Search Results (238)

Zearalenone (ZEN), a mycotoxin commonly found in cereal crops and foods, induces testicular damage and disrupts gut microbial composition. Curcumin (CUR), a bioactive compound derived from turmeric, is known to enhance intestinal microbial balance and exhibit anti-inflammatory properties. This study aimed to investigate the mechanism by which CUR alleviates ZEN-induced reductions in sperm quality through the modulation of the gut microbiota–testis axis. Forty-eight 6-week-old Balb/c male mice were randomly assigned to four treatment groups: control (CON), CUR (200 mg/kg body weight CUR), ZEN (40 mg/kg body weight ZEN), and ZEN + CUR (200 mg/kg CUR + 40 mg/kg ZEN). The degree of sperm damage was quantified by assessing both the survival rate and the morphological integrity of the spermatozoa. CUR was found to mitigate ZEN-induced reductions in the testosterone levels, testicular structural damage, and disrupted spermatogenesis. Exposure to ZEN markedly perturbed the gut microbiota, characterized by increased relative abundances of Prevotella and Bacteroides and a concomitant reduction in Lactobacillus. These alterations were accompanied by pronounced activation of the IL-17A–TNF-α signaling axis, as demonstrated by elevated transcriptional and translational expression of pathway-associated genes and proteins. Co-administration of CUR effectively reinstated microbial homeostasis and mitigated ZEN-induced IL-17A pathway activation. In conclusion, ZEN induces testicular inflammation and reduced sperm quality by lowering testosterone levels and disrupting gut microbial balance, which drives the testicular IL-17A signaling pathway. CUR alleviates ZEN-induced testicular inflammation and sperm quality reduction by restoring beneficial gut microbes and testosterone levels. Full article

Food safety concerns related to pesticide residues in fruits and vegetables have increased globally, particularly in regions where monitoring programs are scarce or inconsistent. This study provides the first multi-year evaluation of pesticide contamination and associated dietary risks in Cape Verde, an African island nation increasingly reliant on imported produce. A total of 570 samples of fruits and vegetables—both locally produced and imported—were collected from major markets across the country between 2017 and 2020 and analyzed using validated multiresidue methods based on gas chromatography coupled to Ion Trap mass spectrometry (GC-IT-MS/MS), and both gas and liquid chromatography coupled to triple quadrupole tandem mass spectrometry (GC-QqQ-MS/MS and LC-QqQ-MS/MS). Residues were detected in 63.9% of fruits and 13.2% of vegetables, with imported fruits showing the highest contamination levels and diversity of compounds. Although only one sample exceeded the maximum residue limits (MRLs) set by the European Union, 80 different active substances were quantified—many of them not authorized under the current EU pesticide residue legislation. Dietary exposure was estimated using median residue levels and real consumption data from the national nutrition survey (ENCAVE 2019), enabling a refined risk assessment based on actual consumption patterns. The cumulative hazard index for the adult population was 0.416, below the toxicological threshold of concern. However, when adjusted for children aged 6–11 years—taking into account body weight and relative consumption—the cumulative index approached 1.0, suggesting a potential health risk for this vulnerable group. A limited number of compounds, including omethoate, oxamyl, imazalil, and dithiocarbamates, accounted for most of the risk. Many are banned or heavily restricted in the EU, highlighting regulatory asymmetries in global food trade. These findings underscore the urgent need for strengthened residue monitoring in Cape Verde, particularly for imported products, and support the adoption of risk-based food safety policies that consider population-specific vulnerabilities and mixture effects. The methodological framework used here can serve as a model for other low-resource countries seeking to integrate analytical data with dietary exposure in a One Health context. Full article

Increased consumer awareness on food safety has spurred the development of detection techniques for pesticide residues. In this study, a rapid detection card on the basis of enzyme action was developed for the visual detection of pesticides, in which the thermally crosslinked and surface-decorated polyvinyl alcohol/citric acid nanofiber mat (PCNM) was employed as a novel immobilization matrix for acetylcholinesterase (AChE). The PCNM, crosslinked at 130 °C for 50 min, exhibited appropriate microstructure and water stability, making it suitable for AChE immobilization. The activation of carboxyl groups by surface decoration resulted in a 2.5-fold increase in enzyme loading capacity. Through parameter optimization, the detection limits for phoxim and methomyl were determined to be 0.007 mg/L and 0.10 mg/L, respectively. The detection card exhibited superior sensitivity and a reduced detection time (11 min) when compared to a commercially available pesticide detection card. Furthermore, the detection results of pesticide residues in fruit and vegetable samples confirmed its feasibility and superiority over commercial alternatives, suggesting its great potential for practical application in the on-site detection of pesticide residues. Full article

The European chickpea market raises concerns about health risks for consumers due to contamination by mycotoxins. Contamination levels can vary depending on the farming system, and rapid and reliable screening tools are desirable. In this study, marketed chickpea seed samples from organic and non-organic farming systems were analyzed for fungal and mycotoxin contamination. Aspergillus and Penicillium were the most frequently identified mycotoxigenic genera. Significant differences in fungal detection were observed among the three isolation methods used, whose combined application is proposed to enhance detection efficiency. The number of Aspergillus and Penicillium colonies was significantly higher in the organic samples. Molecular analysis identified different species within each genus, including several not previously reported in chickpea, as well as potentially aflatoxigenic species such as A. flavus/oryzae and A. parasiticus. LC-MS/MS analysis revealed aflatoxin production only by A. parasiticus, which was present in low amounts. However, the presence of potentially aflatoxigenic Aspergillus species suggests that chickpeas should be monitored to detect their safety and subsequently protect consumer health. A qPCR protocol targeting the omt-1 gene, involved in aflatoxin biosynthesis, proved to be a promising rapid tool for detecting potentially aflatoxigenic Aspergillus species. Full article

Rat hepatitis E virus (rat HEV) is an emerging zoonotic virus detected in rodents worldwide, with increasing evidence of presence in environmental sources such as surface water, wastewater and bivalves. This systematic review compiles and analyzes all the published research on rat HEV contamination in these matrices, as well as its implications for human health. A comprehensive literature search was conducted using databases such as PubMed, Scopus, Web of Science, and Mendeley, including studies published up until 27 May 2025. Studies were included if they evaluated rat HEV in water- or food-related matrices using molecular detection. The risk of bias was not assessed. The certainty of evidence was not formally evaluated. Limitations include reliance on PCR methods without infectivity confirmation. Following PRISMA inclusion and exclusion criteria, eight eligible studies were analyzed. The results show high detection rates of rat HEV RNA in influent wastewater samples from several high-income European countries, namely Sweden, France, Italy, Spain and Portugal. Lower detection rates were found in effluent wastewater and surface waters in Sweden. In bivalve mollusks sampled in Brazil, rat HEV RNA was detected in 2.2% of samples. These findings show the widespread environmental presence of rat HEV, particularly in urban wastewater systems. While human infections by rat HEV have been documented, the true extent of rat HEV zoonotic potential remains unclear. Given the risks associated with this environmental rat HEV contamination, enhanced surveillance, standardized detection methods, and targeted monitoring programs in food production and water management systems are essential to mitigate potential public health threats. Establishing such programs will be crucial for understanding the impact of rat HEV on human health. Full article

Fruits and vegetables are crucial components of a healthy diet, which are susceptible to pests. Therefore, the application of pesticides is a basic manner of crop chemical protection. The aim of this study was a comprehensive analysis of pesticide occurrence in 1114 samples of fruits and vegetables. A unified multi-analytical protocol was used composed of primary–secondary amine/graphitized carbon black/magnesium sulfate to purify samples with diversified profile of interfering substances. Moreover, the obtained analytical data were used to evaluate the critical acute health risk in subpopulations of children and adults within European limits criteria. Out of 550 pesticides analyzed, 38 and 69 compounds were noted in 58.6% of fruits and 44.2% of vegetables, respectively. Acetamiprid (14.1% of all detections) and captan (11.3%) occurred the most frequently in fruits, while pendimethalin (10.6%) and azoxystrobin (8.6%) occurred the most frequently in vegetables. A total of 28% of vegetable and 43% of fruit samples were multiresidues with up to 13 pesticides in dill, reaching a final concentration of 0.562 mg kg⁻¹. Maximum residue level (MRL) was exceeded in 7.9% of fruits and 7.3% of vegetables, up to 7900% MRL for chlorpyrifos in dill (0.79 mg kg⁻¹). Notably, 8 out of 38 pesticides found in fruits (21%; 1.2% for carbendazim) and 24 out of 69 compounds in vegetables (35%, 7.4% for chlorpyrifos) were not approved in the EU. Concentrations of pesticides exceeding MRL were used to assess acute health risk for children and adults. Moreover, the incidence of acute health risk was proved for children consuming parsnip with linuron (156%). In other cases, it was below 100%, indicating that Polish food is safe. The work provides reliable and representative scientific data on the contamination of fruits and vegetables with pesticides. It highlights the importance of legislative changes to avoid the occurrence of not approved pesticides in the EU, increasing food and health safety. Full article

Biogenic amines (BA) are simple organic bases of low molecular weight, formed during decarboxylation of amino acids. Ripened cheeses provide suitable conditions for the development of bacteria and production of BAs. The aim of the present study was to investigate the presence of eight BAs in ripened cheese samples (n = 125) using a high-performance liquid chromatography with diode array detector (HPLC-DAD). Furthermore, microbiological analyses towards identification of bacteria using matrix-assisted laser desorption ionisation—time of flight mass spectrometry (MALDI-TOF MS) were performed. Cadaverine and putrescine were detected in 28.0% and 20.8% of cheese samples at concentrations ranging from 6.12 to 2871 mg/kg and 5.74 to 441 mg/kg, respectively. High amounts of putrescine and cadaverine in cheeses were associated with the presence of Hafnia alvei. Tyramine was identified in 28.0% of samples in the concentration range of 5.62–646 mg/kg. High concentrations of this amine was found in cheeses containing Enterococcus faecium and Enterococcus faecalis. Histamine content, the only BA restricted in food according to Regulation 2073/2005, was observed above 100 mg/kg in 11.2% of the cheeses. Ripened cheeses available on the local retail market may contain significant levels of biogenic amines and may pose a potential health hazard to consumers. Full article

Recent studies have detected microplastics (MPs) in seafood and various food products worldwide, including poultry, fish, salt, beverages, fruits, and vegetables. This widespread contamination makes human exposure through consumption unavoidable and raises concerns for food safety and human health. MPs provide physical support to microorganisms for biofilm formation, protecting them from extreme conditions and facilitating their persistence in the environment. However, little is known about the impact of MPs in the transmission of foodborne pathogens and subsequent spread of infectious diseases like campylobacteriosis, the most common foodborne illness caused by a bacterium, Campylobacter. This review explores the sources of MP contamination in the food chain and offers a comprehensive overview of MP presence in animals, food products, and beverages. Moreover, we compile the available studies linking MPs and Campylobacter and examine the potential impact of these particles on the transmission of Campylobacter along the food chain with a particular focus on poultry, the main source and reservoir for the pathogen. While the environmental and toxicological effects of MPs are increasingly understood, their influence on the virulence of Campylobacter and the spread of antimicrobial resistance remains underexplored. Further studies are needed to develop standardized methods for isolating and identifying MPs, enabling comprehensive investigations and more effective monitoring and risk mitigation strategies. Full article

In this study, the frequency of aflatoxin M1 (AFM1) occurrence in raw milk was investigated across different seasons over a three-year period from 2022 to 2024 in Croatia. Risk assessment was conducted using estimated daily intake (EDI), hazard index (HI), and margin of exposure (MOE) for various age groups and both genders. The frequency of AFM1 detection above the maximum level (ML) ranged from 1.60% to 15.1%. The average incidence of AFM1 exceeding the ML was 5.67%, with the highest incidence recorded in autumn 2024. AFM1 levels within the limit of detection (LOD) and ML were found in 13% of the samples. The average mean value of AFM1 over the three-year period was 19.2 ng/kg. The highest mean AFM1 EDI values were determined for toddlers (0.61–0.67 ng/kg bw/day) and children (0.41–0.43 ng/kg bw/day). The lowest EDI values were observed in elderly females and males (0.058–0.074 ng/kg bw/day). The EDI values for females and males were slightly different. The risk assessment, based on the HI and MOE, indicated that toddlers and children are at the highest risk of exposure to AFM1, which raises significant health concerns. Additionally, consumers of large quantities of milk face a high risk of exposure, particularly during the spring and autumn seasons. For adults and the elderly, milk consumption does not pose a serious health risk. Full article

The main mycotoxin found in apples is patulin (PAT), mostly produced by Penicillium expansum, during the storage of fruits. It is very difficult to control the quality of every fruit that enters the processing line, so there is a high probability that apple juice, applesauce, apple cider, even products intended for babies, contain moldy fruits, with PAT content. This review paper provides detailed information about the extrinsic and intrinsic factors that affect PAT prevalence in apples. Extrinsic factors, such as temperature, air composition in the storage room or packaging material, play a key role in infection with P. expansum and PAT accumulation. Lower temperatures often prevent fungal growth and the production of the mycotoxin, whereas higher or unstable temperatures can promote the buildup of the toxin in infected fruits. Controlled atmosphere storage appears to inhibit the accumulation of PAT in apples. In terms of internal composition, variations in the pH of the fruits and flesh firmness significantly impact fungal growth and PAT production in the fruits. The presence of ethylene, sucrose and polyphenols are some of the decisive chemical components that regulate PAT buildup. Susceptibility of different cultivars is also genetically driven, but the size of the decay area and the toxin-producing capacity of the fungal strain have noteworthy influence as well. Knowledge of these elements helps to understand the mechanisms of PAT production. Full article

This work proposes an insight into the mycotoxins detected in wheat from the 2023 and 2024 harvests in Belgium and highlights the link between agronomic conditions and mycotoxin contamination. The study utilized samples from a Belgian trial network, covering nine locations in 2023 and eight in 2024, ensuring diverse pedoclimatic contexts and including 11 different varieties. Sowing and harvest dates, previous crops and meteorological data were collected for these locations. A validated UPLC-MS/MS multi-mycotoxin method able to detect 20 mycotoxins, regulated or not, was used. Deoxynivalenol, zearalenone, and enniatins B and B1 were detected in the 2023 and 2024 samples. Enniatin A1 was only detected in the 2024 samples. Mycotoxin contamination was higher in 2024 compared to 2023, in terms of both the number of contaminated samples and the contamination levels. Enniatins B and B1, non-regulated mycotoxins, were widely detected in the 2024 wheat samples, with enniatin B detected in 68 out 88 samples ranging from 12 to 488 µg/kg. Differences between the wheat varieties were observed, with some varieties showing significantly higher contamination. Additionally, geographic location appeared to influence contamination levels, which could be related to previous crops or meteorological events. In conclusion, this research provides a comprehensive analysis of mycotoxin co-contamination in wheat samples from diverse pedoclimatic contexts in Belgium based over 2 years. It shows the importance of weather conditions on mycotoxin contamination. It also emphasizes the importance of variety selection to manage mycotoxin contamination. Full article

Due to its nutritional value and versatility, milk is recognized to be one of the most widely consumed beverages worldwide, with a forecasted global consumption of 356 million tons in 2025. Recent updates in legislation and the assessment of their toxicological effects increased attention to the determination of per- and polyfluoroalkyl substances (PFASs), classified as “forever pollutants” for their stability and environmental spread in foods. The present review aims to collect all the information related to milk contamination reported by the scientific literature over the last decade and address the PFAS contamination levels around the globe. Twenty-two studies were found to be published about the detection of PFASs in milk in the period considered in this review, with a total of 824 analyzed samples on a global scale and a maximum of 60 investigated analytes. This review confirms that PFASs, including PFOA and PFOS, are detected in milk worldwide. However, the wide variability in reported concentration levels highlights a critical lack of consistent data and underscores the absence of coordinated, large-scale monitoring programs. This gap in surveillance significantly limits our understanding of actual exposure risks and reinforces the urgent need for comprehensive, systematic research across different regions and production systems. Full article

Heavy metal contamination in food has become a significant global food safety concern. This study assessed the concentrations of As, Ca, Cd, Co, Cr, Cu, Fe, Hg, Mn, K, Mg, Na, Ni, Se, Pb, and Zn in 13 locally grown vegetables using microwave-assisted acid digestion and ICP-MS. The potential human health risks associated with their consumption were also evaluated. Vegetable samples were collected from the local farmer’s market in Grand Forks, North Dakota. The mean levels (μg/g) of Na, Mg, K, Ca, Fe, Se, Mn, Cu, Zn, Co, Hg, Cr, Ni, As, Cd, and Pb were 1001, 2935, 30474, 686.0, 52.90, 0.171, 37.63, 4.936, 21.33, 0.069, 0.0030, 0.049, 0.736, 0.083, 0.298, and 0.019, respectively, having the following decreasing trend: K > Mg > Na > Ca > Fe > Mn > Zn > Cu > Ni > Cd > Se > As > Co > Cr > Pb > Hg. The highest total metals level was found in spinach, with the following decreasing order: spinach > tomato > sugar beet > white eggplant > cucumber ~ kale > green chili > green bean > dill ~ potato > capsicum > onion > corn. Spinach exhibited the highest concentrations of Cd, Cr, Pb, and Hg, which suggests a higher risk of metal exposure from its consumption. Toxic metals except Cd were found to be lower than the maximum allowable concentrations set by international agencies among the analyzed vegetables, while Cd levels were higher than maximum allowable levels in most of the vegetables. Health risks associated with metal intake by vegetable consumption were evaluated in terms of estimated daily intake (EDI), non-carcinogenic risks were evaluated by the target hazard quotient (THQ) and Hazard Index (HI), and carcinogenic risks were evaluated by target cancer risk (TCR). The EDI values of all the metals were found to be below the maximum tolerable daily intake (MTDI). The highest EDI value for Mn, Zn, Hg, Cr, Cd, and Pb was noted in spinach. THQ values for Cd, Co, and As were higher than 1 in most of the vegetable species analyzed, indicating non-carcinogenic health effects to consumers. HI results also posed a non-carcinogenic health risk associated with the intake of these vegetables. Mean TCR values of Cr, Ni, As, and Cd indicated carcinogenic risk for consumers. This study showed that there are potential health risks with consumption of these vegetables. Lastly, regular monitoring of metal levels in vegetables is suggested/recommended to minimize health risks and support pollution control efforts. Full article

Arsenic is a human carcinogen present in drinking water and food, especially rice, rice products and seafood. It can be found in both organic and inorganic forms, the latter being the most toxic. In addition to the carcinogenic effect, exposure to inorganic arsenic can cause numerous disorders in different organs/systems of the human body, such as the skin, cardiovascular, neurological, endocrine, immune, and reproductive systems. The risk assessment associated with dietary arsenic is mainly based on the margin of exposure, i.e., the ratio between the dose at which a small but measurable adverse effect may occur and the estimated daily intake of the target substance. It is mainly influenced by arsenic concentrations and consumption data of average or 95th percentile consumers. This review focuses on the toxicity of arsenic, its sources and routes of human exposure, with particular attention to the ingestion of contaminated water and food, considering the differences between age groups and dietary habits. Full article

Aflatoxin contamination poses a significant food safety risk, particularly during the storage of dried chili peppers. This study evaluated the efficacy of formic acid treatment, ultraviolet (UV) treatment, and combined UV-formic acid treatment in both preventing and controlling Aspergillus flavus in dried red chili powder. Efficacy was assessed by measuring the growth diameter of A. flavus colonies on un-colonized and already colonized dried red chili powder. The optimal treatment conditions for the UV-formic acid combination were determined through single-factor experiments, orthogonal experiments, and quality assessment. Finally, the effects of the UV-formic acid combination on the cell membrane, antioxidant system, and energy metabolism of A. flavus were investigated. The results revealed that fumigation of un-colonized dried red chili powder with 5% formic acid for 24 h inhibited A. flavus growth by 93.29% and toxin synthesis by 99.41%. In contrast, treatment of already colonized chili powder with 10% formic acid inhibited A. flavus colony growth by 50%. Through a three-factor, three-level orthogonal experiment followed by quality testing, the optimal conditions were determined to be 8% formic acid concentration, a UV irradiation distance of 15 cm, and a treatment time of 75 min. This optimized combined treatment reduced the required fumigation time from 24 h to 1.25 h. This technique achieved complete suppression of aflatoxin B₁ synthesis on un-colonized dried red chili powder. On already colonized chili powder, the mycelial growth inhibition rate was 48.05 ± 6.68%, and aflatoxin B₁ synthesis was inhibited by 91.32 ± 3.15%. Quality assessment revealed that the UV-formic acid co-treatment parameters did not significantly affect key quality indicators including color, capsaicin content, total phenolic content (p > 0.05). Furthermore, UV-formic acid treatment disrupt the cell membrane structure of A. flavus, impairs its antioxidant and energy metabolism systems, and induces mitochondrial dysfunction. The study confirmed the synergistic antifungal effect of formic acid and UV, providing a potential industrialized solution for enhancing the safety and storage stability of dried chili products. Full article