Search Results (99)

Artificial gynogenesis is an essential technique for aquaculture breeding. Fertile offspring of the WuLi carp (Cyprinus carpio var. Quanzhounensis, 2n = 100, WLC) were successfully produced via gynogenesis using ultraviolet-irradiated sperm from the blunt snout bream (Megalobrama amblycephala, 2n = 48, BSB). As anticipated, gonadal section examination confirmed that all gynogenetic WuLi carp (2n = 100, GWB) were female. To investigate whether paternal DNA fragments from BSB were integrated into the GWB genome, comparative analyses of morphological traits, DNA content, chromosomal numbers, 5S rDNA sequences, microsatellite DNA markers, fluorescence in situ hybridization (FISH), growth performance and nutritional composition were systematically conducted between GWB and maternal WLC. The results revealed pronounced maternal inheritance patterns across morphological characteristics, DNA quantification, chromosomal configurations, 5S rDNA sequences and FISH signals, while microsatellite detection unequivocally confirmed paternal BSB DNA fragment integration into the GWB genome. Remarkably, GWB demonstrated significantly superior growth performance and elevated unsaturated fatty acid content relative to the maternal line. This approach not only addressed germplasm degradation in WLC but also provided valuable theoretical foundations for breeding programs in this commercially significant species. Full article

Based on the new high-resolution multibeam bathymetry data collected by the “Dongfanghong 3” vessel in 2023 in the Parece Vela Basin (PVB) and previous magnetic anomaly data, we systematically analyze the seafloor topographical changes of abyssal hills and oceanic core complexes (OCCs) in the “Chaotic Terrain” region, and the revised seafloor spreading model is constructed in the PVB. Using detailed analysis of the seafloor topography, we identify typical geomorphological features associated with seafloor spreading, such as regularly aligned abyssal hills and OCCs in the PVB. The direction variations of seafloor spreading in the PVB are closely related to mid-ocean ridge rotation and propagation. The formation of OCCs in the “Chaotic Terrain” can be explained by links to the continuous and persistent activity of detachment faults and dynamic adjustments controlled by variations of deep magma supply in the different segments in the PVB. We use 2D discrete Fourier image analysis of the seafloor topography to calculate the aspect ratio (AR) values of abyssal hills in the western part of the PVB. The AR value variations reveal a distinct imbalance in magma supply across various regions during the basin spreading process. Compared to the “Chaotic Terrain” area, the region with abyssal hills indicates a higher magma supply and greater linearity on seafloor topography. AR values fluctuated between 2.1 and 1.7 of abyssal hills in the western segment, while in the “Chaotic Terrain”, they dropped to 1.3 due to the lower magma supply. After the formation of the OCC-1, AR values increased to 1.9 in the eastern segment, and this shows the increase in magma supply. Based on changes in seafloor topography and variations in magma supply across different segments of the PVB, we propose that the seafloor spreading process in the magnetic anomaly linear strip 9-6A of the PVB mainly underwent four formation stages: ridge rotation, rift propagation, magma-poor supply, and the maturation period of OCCs. Full article

A low-profile dual-polarized shared-aperture phased array antenna is proposed for Ku-band satellite communications in this paper. The stacked octagonal patches loaded with Via-rings are proposed as dual-polarized shared-aperture radiation elements, with the characteristics of wide impedance bandwidth, high gain, and weak coupling. Furthermore, innovative minimized three-port ring couplers are utilized for the differential-fed antenna array, further suppressing the cross-polarization component. Substrate integrated coaxial line (SICL) and microstrip line (MS) feed networks are employed for the excitation of transmitting band (Tx) horizontal polarization and receiving band (Rx) vertical polarization, respectively. The non-uniform subarray architecture is optimized to minimize the sidelobe levels with the reduced number of transmitter and receiver (T/R) radio frequency phase-shifting modules. As proof-of-concept examples, 16 × 24 and 32 × 24 array antennas are demonstrated and fabricated. The measured impedance bandwidths of the proposed phased array antennas are around 21.1%, while the in-band isolations are above 36.7 dB. Gains up to 29 dBi and 32.4 dBi are performed by two prototypes separately. In addition, the T/R phase-shifting modules are utilized to validate the beam-scanning characteristic, which is of value for dynamic satellite communications. Full article

This study investigated the effects of alternating current (AC) interference on pipeline steel under cathodic protection (CP). In a simulated solution, real-time electrochemical measurements and corrosion rate analysis were conducted on two steel types (C1018 and X60) under various levels of AC interference with CP. Due to the complexity of AC-induced corrosion, relying on the shift in DC potential alone cannot accurately demonstrate the corrosion behavior in the presence of AC interference. In fact, such an approach may mislead the predictions of corrosion performance. It is observed that AC interference reduced the effectiveness of CP and increased the corrosion rate of the steel, both in weight loss and Tafel Extrapolation (Tafel) measurements. The study concluded that conventional CP standards used in the field were inadequate in the presence of high AC-level interference. Furthermore, this study found that a more negative CP current density (−0.75 A/m²) could reduce the effect of AC interference by 46–93%. This is particularly shown in the case of low-level AC interference, where the reduction can reach up to 93%. Utilizing the experimental data obtained by the two measurement methods, probabilistic models to predict the corrosion rate were developed with consideration of the uncertainty in the measurements. The sensitivity analysis showed how AC interference impacts the corrosion rate for a given CP level. Full article

The density of SLMed (Selective Laser Melting) Ti-22Al-25Nb alloy was improved through hot isostatic pressing (HIP) treatment, and the influence of HIP and solution aging on the microstructure of Ti-22Al-25Nb alloy in the as-deposited state was examined. The results indicate that following (1100 °C + 300 MPa)/3 h-HIP, the specimen densities have risen to 99.71%, porosity has markedly decreased, and internal flaws have been eradicated. Microstructural analysis reveals a significant presence of GBα₂ (GB, Grain Boundary) along grain boundaries, with GBLO + α₂ (GBL, Grain Boundary Lath; O, Orthorhombic) laths extending parallel from the grain boundaries into the intragranular region. Additionally, a limited number of cross or snowflake O + α₂ lath clusters and acicular O phases are precipitated within the B2 (B, Body-centered cubic) phase in the HIPed state, characterized by isotropic and linear grain boundaries. The GBLα₂ and GBLO exhibit two growth modes: sympathetic nucleation and interfacially unstable nucleation. During the solid solution treatment following HIP, as the solid solution temperature rises, the acicular O phase, GBLO, lath O phase, lath α_2, and GBα₂ sequentially dissolve, increasing the volume fraction of the B2 phase. After HIP, the aging microstructure is primarily characterized by the proliferation of the acicular O phase precipitated from the B2 phase and retaining the lath O phase in a solid solution. The precipitation of GBLO in the original solid solution is suppressed, and the GBLα₂ in the original solid solution partially decomposes into rimO, resulting in coarse grain size and significant internal decomposition of α₂. Following solution treatment and aging at 920 °C, the proliferation of the acicular O phase enhances ductility, resulting in ideal overall characteristics with a yield strength (YS) of 760.81 MPa, ultimate tensile strength (UTS) of 869.32 MPa, and elongation (EL) of 2.683%. This study demonstrates that the HIP treatment and the modification of solution aging parameters can substantially increase the density and refine the microstructure of Ti-22Al-25Nb alloy, hence enhancing its mechanical properties. Full article

Zn-Ag alloys are deemed extremely promising materials for manufacturing biodegradable medical implants. Nonetheless, their practical applications are still constrained by inferior mechanical properties. To tackle this issue, Zn-0.5Ag alloy was alloyed with Mg (0.2 wt.%) and processed by combined equal-channel angular pressing (ECAP) and rolling, with different rolling reductions (40%, 60%, and 75%). ECAP-processed Zn-0.5Ag-0.2Mg alloy exhibited superior mechanical properties to its as-cast counterpart. Subsequent rolling of 40% further enhances the mechanical performance of ECAP-processed Zn-0.5Ag-0.2Mg alloy, with yield strength (YS), ultimate tensile strength (UTS), and elongation (EL) reaching 255 MPa, 309 MPa, and 52%, respectively, surpassing the application requirements. As the rolling reduction increased to 60% and further to 75%, YS and UTS declined, whereas EL rose continuously. The underlying mechanisms for the variation in strength and ductility were elucidated based on microstructure evolution analysis through optical microscopy (OM), scanning electron microscopy (SEM), and electron backscatter diffraction (EBSD) characterizations. Full article

The rural landscape serves as a window to showcase regional culture and can drive the development of the rural cultural tourism industry. However, driven by the rural revitalization strategy, the construction of rural landscapes in the Yangtze River Delta region faces the challenges of homogeneity and lack of authenticity. A regional evaluation of the rural landscape and strategic suggestions are key to solving this problem. Therefore, this study selected three representative villages in the Yangtze River Delta region and established a regional evaluation model of the rural landscape in the Yangtze River Delta from the perspective of the ecological–production–living concept, utilizing the analytic hierarchy process, a tourist questionnaire survey, IPA, and Munsell color analysis. The results show that (1) the core indicator of the rural landscape regionality is the life landscape, followed by the production landscape, and finally, the ecological landscape; (2) the overall satisfaction of the rural landscape is high, and the satisfaction of the water network landscape is significantly higher than other indicators; (3) the results of IPA show that what needs to be maintained are traditional dwellings and historical relics, and what needs to be improved are sign design and rural public art design; (4) Munsell color analysis shows that the characteristics the of rural landscape in the Yangtze River Delta region are diverse and inclusive. This study is of great significance for maintaining the characteristics of the rural landscape in the Yangtze River Delta region and promoting the protection of rural landscape style under different regional conditions. Full article

The diamide insecticide tetraniliprole is a valuable tool for managing major insect pests like the invasive tomato pinworm, Tuta absoluta (Meyrick). However, the mechanisms underlying tetraniliprole resistance, as well as its associated fitness costs, remain unclear. In this study, we assessed the fitness of tetraniliprole-resistant (TetraRS) and susceptible (SS) strains of T. absoluta and conducted Illumina RNA-seq to compare their transcriptomes. We also used nanocarrier-mediated RNA interference (RNAi) to knockdown P450 genes and evaluate their role in tetraniliprole resistance. After eight generations of selection, T. absoluta developed a 20.80-fold resistance to tetraniliprole, accompanied by fitness costs. RNA-seq analysis revealed 3332 differentially expressed genes (DEGs), with 1707 upregulated and 1625 downregulated in the TetraRS compared to the SS strain. Gene Ontology (GO) annotations showed significant enrichment in categories related to metabolic processes, cellular processes, catalytic activity, cellular anatomical entity, and binding. These genes were also identified in key KEGG pathways such as cytochrome P450, drug metabolism, carbon metabolism, oxidative phosphorylation, fatty acid metabolism, and protein processing. RT-qPCR analysis confirmed that P450 genes (CYP405D1, CYP6AB269, and CYP4AU1) were upregulated in TetraRS insects, in line with the RNA-seq results. Cytochrome P450 activity was significantly higher in the TetraRS strain than in the SS strain. Notably, nano-encapsulated dsRNA targeting these overexpressed P450 genes increased the susceptibility of T. absoluta to tetraniliprole. Further, cytochrome P450 activity was significantly reduced following silencing of P450 genes. These findings suggest that multiple genes and pathways, particularly P450 genes, contribute to tetraniliprole resistance in T. absoluta. This study provides valuable insights into the molecular mechanisms underlying insecticide resistance in this key pest species. Full article

20-hydroxyecdysone (20E) is essential for insect development and diapause. Ecdysone receptor (EcR) and ultraspiracle (USP) proteins are crucial regulators of 20E signaling. To explore their potential roles in the development of Sitodiplosis mosellana, a major wheat pest that undergoes obligatory diapause as a larva, one SmEcR and two SmUSPs (SmUSP-A and SmUSP-B) from this species were isolated and characterized. The deduced SmEcR and SmUSP-A/B proteins contained a conserved DNA-binding domain with two zinc finger motifs that bind to specific DNA sequences. Expression of SmEcR and the SmUSPs was developmentally controlled, as was 20E induction. Their transcription levels increased as the larvae entered pre-diapause, followed by downregulation during diapause and upregulation during the shift to post-diapause quiescence, which is highly consistent with ecdysteroid titers in this species. Topical application of 20E to diapausing larvae also elicited a dose-dependent expression of the three genes. Expression of SmEcR and SmUSPs decreased markedly during the pre-pupal stage and was higher in adult females compared to males. These findings suggested that 20E-induced expression of SmEcR and SmUSPs has key roles in diapause initiation and maintenance, post-diapause quiescence, and adult reproduction, while the larval–pupal transformation may be associated with a decrease in their expression levels. Full article

Background and Objectives: Identifying carriers of Pathogenic/Likely Pathogenic Variants in patients with dementia is crucial for risk stratification, particularly in individuals with a family history. This study developed and validated a clinical prediction model using whole-exome sequencing-confirmed cohorts. Methods: A total of 601 Chinese patients with dementia and a family history were enrolled at Peking Union Medical College Hospital, with 476 in a retrospective derivation cohort and 125 in a temporal validation cohort. Predictive factors included age at onset, APOE ε4 status, and family history characteristics. Model performance was assessed using discrimination and calibration metrics. Results: In the derivation cohort (median age at onset 66 years), 10.3% carried Pathogenic/Likely Pathogenic Variants. Among patients with dementia, those with age at onset < 55 years (OR 2.56, p = 0.0098), more than two affected relatives (OR 3.32, p = 0.0039), parental disease history (OR 4.72, p = 0.015), and early-onset cases in the family (OR 2.61, p = 0.0096) were positively associated with Pathogenic/Likely Pathogenic Variant carriage, whereas APOE ε4 carriage was inversely associated (OR 0.36, p = 0.0041). The model achieved an area under the curve of 0.776 (95% CI, 0.701–0.853) in the derivation cohort and 0.781 (95% CI, 0.647–0.914) in the validation cohort (median age at onset 58 years), with adequate calibration. Conclusions: This model demonstrated strong predictive performance for Pathogenic/Likely Pathogenic Variant carriage, supporting its clinical utility in guiding genetic testing. Further research is needed to refine the model. Full article

In the casting and stamping process of automobile, ship, aerospace, and other fields, improving the atomization quality of the spray release agent can effectively solve the problems of difficult film removal, low efficiency, and poor surface finish, and greatly improve the efficiency of production and manufacturing. The geometric model of the external mixing nozzle was constructed, and the calculation domain and grid were divided. The atomization flow field velocity, liquid film thickness, particle distribution, and cooling amount were calculated using fluid simulation software. Finally, an experimental platform was set up for verification. With the increase in the distance between the iron plate and the nozzle, the velocity of the flow field decreases from the nozzle exit to the periphery, and the frequency distribution of D_60–70 increases gradually. With the increase in the pressure ratio (K), the particle velocity increases gradually, the liquid film thickness increases first, and then gently decreases, and the D_60–70 frequency distribution decreases. The influence of gas pressure on atomized particle velocity and liquid film thickness is greater than that of liquid phase pressure, and the ion velocity reaches the peak value when K = 2. When K = 1.5, the average thickness increment of absolute liquid film is small, the atomized particle diameter changes the least, the frequency distribution of D₆₅ particles is approximately the same, and the atomization effect is the most stable. When the spraying time is 1 s, the K value is larger, and the smaller the temperature drop will be. In 2–4 s, the change in K value has little influence on the cooling amount. Full article

The gut–liver axis is essential in animal disease and health. However, the role of the gut–liver axis in the anti-disease mechanism of disease-resistant grass carp (DRGC) derived from the backcross of female gynogenetic grass carp (GGC) and male grass carp (GC) remains unclear. This study analyzed the changes in gut histopathology, fecal intestinal microflora and metabolites, and liver transcriptome between GC and DRGC. Histological analysis revealed significant differences in the gut between DRGC and GC. In addition, microbial community analyses indicated that hybridization induced gut microbiome variation by significantly increasing the proportion of Firmicutes and Bacteroidota in DRGC. Metabolomic data revealed that the hybridization-induced metabolic change was probably characterized by being related to taurocholate and sphinganine in DRGC. Transcriptome analysis suggested that the enhanced disease resistance of DRGC was primarily attributed to immune-related genes (SHMT2, GOT1, ACACA, DLAT, GPIA, TALDO1, G6PD, and FASN). Spearman’s correlation analysis revealed a significant association between the gut microbiota, immune-related genes, and metabolites. Collectively, the gut–liver axis, through the interconnected microbiome–metabolite–gene pathway, may play a crucial role in the mechanism of greater disease resistance in DRGC, offering valuable insights for advancing the grass carp cultivation industry. Full article

The exploration of visible light-responsive, efficient, and durable photocatalysts is of great concern for removing organic dyes and antibiotics from wastewater. This work involved the preparation of a CoCN0.02 photocatalyst by simple thermal polymerization. The synthesized catalysts were mainly used for the photocatalytic degradation of tetracycline (TC) pollutants. The photocatalytic efficiency of one of the catalysts reached 97% in 30 min, which was much higher than that of pure g-C₃N₄ (CN). The consistency between the results of kinetic simulations and characterization supported the strong role of Co intercalation sites in photocatalysis. Additionally, using the active species capture experiments, the predominant active species were determined to be •OH, •O₂⁻, and h⁺, thereby allowing us to explore the electron transportation and redox reactions during the process of photocatalysis. This investigation establishes a basis for exploring the evolution of active species in the context of antibiotic pollutants. Full article

To achieve rapid distance estimation and tracking of moving targets in a large field of view, this paper proposes an innovative simulation method. Using a low-cost approach, the imaging and distance measurement performance of the designed cooling-type mid-wave infrared compound-eye camera (CM-CECam) is experimentally evaluated. The compound-eye camera consists of a small-lens array with a spherical shell, a relay optical system, and a cooling-type mid-wave infrared detector. Based on the spatial arrangement of the small-lens array, a precise simulation imaging model for the compound-eye camera is developed, constructing a virtual imaging space. Distance estimation and error analysis for virtual targets are performed using the principle of stereo disparity. This universal simulation method provides a foundation for spatial design and image-plane adjustments for compound-eye cameras with specialized structures. Using the raw images captured by the compound-eye camera, a scene-specific piecewise linear mapping method is applied. This method significantly reduces the brightness contrast differences between sub-images during wide-field observations, enhancing image details. For the fast detection of moving targets, ommatidia clusters are defined as the minimal spatial constraint units. Local information at the centers of these constraint units is prioritized for processing. This approach replaces traditional global detection methods, improving the efficiency of subsequent processing. Finally, the simulated distance measurement results are validated using real-world scene data. Full article

The Dof gene family represents a class of plant-specific transcription factors that play crucial regulatory roles in various biological processes, including plant growth, development, and responses to abiotic stress. However, genome-wide identification and functional characterization of the Dof gene family remain unexplored in Dendrobium officinale. In this study, we performed a genome-wide identification and functional analysis of the DoDof gene family. A total of 28 Dof family members were identified and named DoDof1–28 based on genome annotation data. Phylogenetic analysis classified these genes into four major groups (A–D) and further subdivided them into nine subfamilies. Gene structure analysis revealed that most DoDofs lack introns, with no distinct specificity observed among different subfamilies and considerable diversity within the same subfamily. Sequence alignment analysis demonstrated that all DoDof proteins contain a conserved Dof domain consisting of 52 amino acids, which includes a C2-C2 zinc finger motif and a DNA-binding domain. MEME analysis revealed that the conserved motif composition exhibits a certain degree of conservation among DoDof proteins, but significant differences exist across subfamilies. Expression pattern analysis demonstrated that DoDofs have exhibited diverse expression profiles across different developmental stages, tissues, and under abiotic stresses (such as low temperature, salinity, and drought) in D. officinale, suggesting their potential roles in plant development and stress responses. Subcellular localization analysis indicated that DoDof15, DoDof22, and DoDof24 are localized exclusively in the nucleus. Yeast one-hybrid assays revealed that DoDof22 binds to the promoter of the ABA receptor DoPYL9, while DoDof15 and DoDof24 bind to the promoter of the bHLH transcription factor DobHLH68. These results suggest that DoDof proteins may regulate the growth, development, and stress response processes of D. officinale by binding to the promoters of target genes. This study provides critical insights into the functional roles of Dof transcription factors in Orchidaceae family and establishes a theoretical foundation for molecular breeding and stress resistance improvement in D. officinale. Full article