Search Results (14)

Nipah virus (NiV) is a severe zoonotic pathogen that substantially threatens public health. Pigs are the natural hosts of NiV and can potentially transmit this disease to humans. Establishing a rapid, sensitive, and accurate point-of-care detection method is critical in the timely identification of infected pig herds. In this study, we developed an NiV detection method based on reverse transcription–recombinase polymerase amplification (RT-RAA) and the clustered regularly interspaced short palindromic repeats (CRISPR)-associated protein 13a (Cas13a) system for the precise detection of NiV. The highly conserved region of the NiV gene was selected as the detection target. We first designed eleven pairs of RT-RAA primers, and the optimal primer combination and reaction temperature were identified on the basis of RT-RAA efficiency. Additionally, the most efficient crRNA sequence was selected on the basis of the fluorescence signal intensity. The results revealed that the optimal reaction temperature for the developed method was 37 °C. The detection limit was as low as 1.565 copies/μL. Specificity testing revealed no cross-reactivity with nucleic acids from six common swine viruses, including Seneca virus A (SVA), foot-and-mouth disease virus (FMDV), classical swine fever virus (CSFV), porcine epidemic diarrhea virus (PEDV), African swine fever virus (ASFV), and pseudorabies virus (PRV). A validation test using simulated clinical samples revealed a 100% concordance rate. The detection results can be visualized via a fluorescence reader or lateral flow strips (LFSs). Compared with conventional detection methods, this RT-RAA-CRISPR/Cas13a-based method is rapid and simple and does not require scientific instruments. Moreover, the reagents can be freeze-dried for storage, eliminating the need for cold-chain transportation. This detection technology provides a convenient and efficient new tool for the point-of-care diagnosis of NiV and for preventing and controlling outbreaks. Full article

This study used polyacrylonitrile (PAN) and heat-treated polyacrylonitrile (H-PAN) membranes to enrich nutmeg essential oils, which have more complex compositions compared with common oils. The oil rejection rate of the H-PAN membrane was higher than that of the PAN membrane for different oil concentrations of nutmeg essential oil-in-water emulsions. After heat treatment, the H-PAN membrane showed a smaller pore size, narrower pore size distribution, a rougher surface, higher hydrophilicity, and higher oleophobicity. According to the GC-MS results, the similarities of the essential oils enriched by the PAN and H-PAN membranes to those obtained by steam distillation (SD) were 0.988 and 0.990, respectively. In addition, these two membranes also exhibited higher essential oil rejection for Bupleuri Radix, Magnolia Officinalis Cortex, Caryophylli Flos, and Cinnamomi Cortex essential oil-in-water emulsions. This work could provide a reference for membrane technology for the non-destructive separation of oil with complex components from oil-in-water emulsions. Full article

Plastic film mulching, widely used in agriculture, leads to microplastic (MP) pollution in soils. While biodegradable polybutylene adipate terephthalate (PBAT) films may offer a solution, their impacts on subsurface soils and microorganisms remain unclear. To investigate the effects of conventional non-biodegradable polyethylene (PE) and biodegradable PBAT MPs on the properties of sub-surface soils and microbial communities, MPs were added at varying doses in a field experiment and incubated for 160 days. Physicochemical characteristics, nutrient dynamics, and microbial composition, diversity, and networks of soils were analyzed using standard techniques and 16S rRNA/ITS gene sequencing. Correlations between soil properties and microbes were assessed. Both MP types significantly altered soil characteristics, with PBAT-MP elevating pH and the levels of available phosphorus and potassium more than PE-MP. Microbial composition shifts occurred, with low-addition PBAT-MP promoting plastic-degrading genera. The assessment of α/β-diversity indicated that PBAT-MP predominantly influenced fungi while PE-MP impacted bacteria. An examination of microbial co-occurrence networks highlighted that PE-MP primarily disrupted fungal interactions, whereas PBAT-MP streamlined network complexity. Correlation analyses revealed that PBAT-MP promoted fungal diversity/network resilience correlating to nutrients. PE-MP and PBAT-MP significantly altered native soil/microbe relationships. PBAT-MP may exert greater, yet unknown, impacts over time through its biodegradation into newer and smaller fragments. Future research needs to integrate multi-omics and stable isotope science to elucidate the deep mechanistic impacts of degraded film-derived MPs on microbial ecological functions and biogeochemical cycles. Attention should also be paid to the long-term accumulation/transport of MPs in agricultural soils. Overall, this work deepens the impact and understanding of MPs from plastic film on sub-surface soil ecology. Furthermore, it provides a theoretical foundation for managing ‘white pollution’ in the film-covered farmlands of arid and semi-arid regions in China. Full article

In recent years, the influence of extreme weather patterns has led to an alarming increase in the frequency and severity of sub-surface forest fires in boreal forests. The Ledum palustre-Larix gmelinii forests of the Daxing’an Mountains of China have emerged as a hotspot for sub-surface fires, and terrain slope has been recognized as a pivotal factor shaping forest fire behavior. The present study was conducted to (1) study the effect of terrain slope on the smoldering temperature and spread rate using simulated smoldering experiments and (2) establish occurrence probability prediction model of the sub-surface fires’ smoldering with different slopes based on the random forest model. The results showed that all the temperatures with different slopes were high, and the highest temperature was 947.91 °C. The spread rates in the horizontal direction were higher than those in the vertical direction, and the difference increased as the slope increased. The influence of slope on the peak temperature was greater than that of spread rate. The peak temperature was extremely positively correlated with the slope, horizontal distance and vertical depth. The spread rate was extremely positively correlated with the slope. The spread rate in the vertical direction was strongly positively correlated with the depth, but was strongly negatively correlated with the horizontal distance; the horizontal spread rate was opposite. The prediction equations for smoldering peak temperature and spread rate were established based on slope, horizontal distance, and vertical depth, and the model had a good fit (p < 0.01). Using random forest model, we established the occurrence prediction models for different slopes based on horizontal distance, vertical depth, and combustion time. The models had a good fit (AUC > 0.9) and high prediction accuracy (accuracy > 80%). The study proved the effect of slope on the characteristics of sub-surface fire smoldering, explained the variation in peak temperature and spread rate between different slopes, and established the occurrence prediction model based on the random forest model. The selected models had a good fit, and prediction accuracy met the requirement of the sub-surface fire prediction. Full article

With the emergence of drug resistance and the consequential high morbidity and mortality rates, there is an urgent need to screen and identify new agents for the effective treatment of cancer. Terphenyls—a group of aromatic hydrocarbons consisting of a linear 1,4-diaryl-substituted benzene core—has exhibited a wide range of biological activities. In this study, we discovered a terphenyllin derivative—CHNQD-00824—derived from the marine compound library as a potential anticancer agent. The cytotoxic activities of the CHNQD-00824 compound were evaluated against 13 different cell lines with IC₅₀ values from 0.16 to 7.64 μM. Further study showed that CHNQD-00824 inhibited the proliferation and migration of cancer cells, possibly by inducing DNA damage. Acridine orange staining demonstrated that CHNQD-00824 promoted apoptosis in zebrafish embryos. Notably, the anti-cancer effectiveness was verified in a doxycin hydrochloride (DOX)-induced liver-specific enlargement model in zebrafish. With Solafinib as a positive control, CHNQD-00824 markedly suppressed tumor growth at concentrations of 2.5 and 5 μM, further highlighting its potential as an effective anticancer agent. Full article

A sub-surface forest fire is a type of smoldering combustion with a slower spread rate, longer combustion time, and lower combustion temperature compared with flame combustion. Sub-surface fires are usually accompanied by surface fires, and the surface fires’ conversion from sub-surface fires has great uncertainty. Therefore, there are considerable difficulties in monitoring and fighting sub-surface fires. However, there are few studies on the conversion from sub-surface fires to surface fires, and the mechanism and influencing factors of the conversion remain unclear. This study focuses on Larix gmelinii forests, which are representative of the boreal forest of China and hot spots of sub-surface fires, studies the moisture content limit of sub-surface fires’ conversion to surface fires by simulating a smoldering experiment, and establishes a monitoring model of sub-surface fires and an occurrence probability prediction model of sub-surface fires’ conversion to surface fires. The results showed that the moisture content limit of the conversion was 25% in the grass–Larix gmelinii forest and Ledum palustre–Larix gmelinii forest and 20% in Rhododendron dauricum–Larix gmelinii forest. There was a significant positive correlation between the time and temperature caused by the smoldering. The monitoring model of the sub-surface fires based on the surface temperature and moisture content had a good fitting effect (p < 0.01). The occurrence probability prediction model of the sub-surface fires’ conversion to surface fires, based on a logistic regression model, had high prediction accuracy (AUC = 0.987). The lower the moisture content of the humus, the closer the smoldering came to the surface and the higher the probability of conversion. This research could contribute to the study of the mechanism of sub-surface fires’ conversion into surface fires. Full article

With its excellent electrical and mechanical properties and the rapid development of its device fabrication technologies, laser-induced graphene (LIG) has played an important role in the field of wearable technologies since its discovery in 2014. In recent years, with the relentless development of wearable devices, newly developed LIG-based wearable devices also possess multifunction and intelligence characteristics. This review is aimed toward two of the most important fields related to the development of LIG, namely, health care and human–computer interaction (HCI). We introduce multifunctional and intelligent LIG-based wearable systems for health care and HCI developed over the recent years, sorting out their design ideas, preparation process, performance, and application. Furthermore, we discuss the future development direction of LIG-based wearable systems for health care and HCI. Full article

Smouldering combustion can emit a large amount of CO₂, CO and particulate matter (PM). Moisture content is an important factor of the emission characteristics. As the hot spot of forest smouldering combustion, the gas and particulate emissions of the Huzhong National Nature Reserve with different moisture contents are discussed herein. The emission factors (EF) of CO₂ and CO were 100.71 ± 39.14 g/kg and 11.76 ± 3.89 g/kg, respectively. The EF of PM_2.5, PM₄ and PM₁₀ were 87.11 ± 19.47 g/kg, 353.37 ±159.25 g/kg and 602.59 ± 276.80 g/kg, respectively. PM_2.5 accounted for 16.59 ± 5.25% of the PM, and PM₄ and PM₁₀ were 54.03 ± 13.46% and 91.00 ± 10.81%, respectively. There was no significant difference in the EF of CO₂ and CO with different moisture contents, nor in the EF of PM_2.5, but there was a significant difference in the EF of PM₄ and PM₁₀ with different moisture contents. In addition, the peak of CO₂ and CO appeared at 2~3 h; the peak of PM_2.5 lagged behind that of PM₄ and PM₁₀. According to the regression analysis, experimental expressions were obtained for the modified combustion efficiency (MCE) and the EF of PM. Full article

Background: Patients with Crohn’s disease (CD) undergoing bowel resection often suffer from depression and acute pain, which severely impairs their recovery. We aimed to investigate the effects of S-ketamine preconditioning on postoperative depression in patients with CD undergoing a bowel resection with mild to moderate depression and to observe whether it can relieve postoperative pain and anti-inflammation. Methods: A total of 124 adult patients were randomized into one of the two groups. Patients in the S-ketamine group received a 0.25 mg/kg S-ketamine intravenous drip under general anesthesia induction, followed by a continuous infusion of S-ketamine with 0.12 mg/kg/h for more than 30 min through target-controlled infusion. Patients in the placebo group received 0.9% saline at an identical volume and rate. The primary outcome measure was the 17-item Hamilton depression Scale (HAMD-17). The secondary outcomes were scores on the following questionnaires: a nine-item patient health questionnaire (PHQ-9); a quality of recovery (QoR-15) form; and a numeric rating scale (NRS). Additional secondary outcomes included the levels of C-reactive protein (CRP) and interleukin 6 (IL-6) on postoperative days (PODs) 1, 3, and 5, the length of hospital stay, and opioid use throughout the hospital stay. Results: The scores of PHQ-9 and HAMD-17 in the S-ketamine group were lower than those in the placebo group on postoperative days (PODs) 1, 2, and 7 (p < 0.05). The scores of QoR-15 in the S-ketamine group were higher than those in the placebo group on postoperative days (PODs) 3 and 5 (p < 0.05). The NRS scores of PACU, postoperative days 1 and 2 in the S-ketamine group were lower than those in the placebo group (p < 0.05). There was no significant difference in the CRP and IL-6 levels on postoperative days (PODs) 1, 3, and 5, postoperative complications, and hospital stay between the two groups (p > 0.05). Conclusions: The trial indicated that the intraoperative administration of low-dose S-ketamine could alleviate mild-to-moderate depressive symptoms and postoperative pain in patients with Crohn’s disease undergoing bowel resection without worsening their safety. Full article

A sub-surface forest fire is a kind of fire that spreads slowly with no flames and lower temperatures, and threatens the ecosystem and human life. The moisture content of humus is considered to be an important factor in determining fire occurrence and sustaining. The humus of the Larix gmelinii in the Daxing’an Mountains was selected for the experiment, the limit moisture content condition of sub-surface forest fires was determined by an experiment simulating smoldering, and the prediction model of the probability of sub-surface forest fire occurrence was established. The results will be of great significance for the prevention, monitoring, and fighting of sub-surface forest fires in the boreal forest. The results showed that when the moisture content of humus in the upper layer was low, the smoldering process could be self-sustaining at 20%. For deeper layers of a depth of 18 cm, this increased to 30% moisture content of the humus and was the critical depth for sub-surface fires. The moisture content of 40% was a limit to burning where smoldering can only last for a short duration and is then extinguished. When the moisture content of the humus was 20%, the smoldering temperature was higher and the rate of spread was faster, with smoldering being maintained for longer periods at 30% moisture content. The regression prediction model of the highest temperature and vertical rate of spread in a column of humus was correlated to moisture content and depth, and the model significance was good at p < 0.01. Based on moisture content and depth, the occurrence probability prediction model of sub-surface fires has a good correlation (R² = 0.93) and high prediction accuracy (AUC = 0.995). The effect of moisture content (Or = 4.008) on the occurrence probability of sub-surface fires is higher than that of depth (Or = 2.948). The results point out that it is necessary to prevent and monitor the occurrence of sub-surface fires when the humus moisture content is less than 40%. In order to reduce the risk of sub-surface fires, the monitoring time of the fire field should be extended after the fire is extinguished due to the slow-burning process of the sub-surface fire. Increasing the moisture content of the humus is an important method to reduce the probability and restrain the spread of sub-surface fires. Full article

To resolve problems caused by the accumulation of flue gas desulfurization gypsum (FGDG) in the environment, a polymer material was prepared using FGDG, granulated blast furnace slag (GBFS), fly ash (FA), and solid sodium silicate (SSS). The compressive strength of these polymer specimens cured for 3, 28, and 60 d was regularly measured, and their condensation behavior was analyzed. Both the formation behavior of mineral crystals and microstructure characteristics were analyzed further using X-ray diffraction and scanning electron microscopy. The compressive strength of pure FGDG polymer specimen (whose strength is generated by particle condensation crystallization) is insufficient and the condensation is slow. The addition of appropriate amounts of GBFS, FA, and SSS can continuously and considerably improve the compressive strength and shorten the setting time. The optimal proportions of FGDG, GBFS, and FA are 50%, 20%, and 30%, respectively, with the SSS addition amount of 20 g. The incorporation of GBFS, FA, and SSS can promote the polymerization of calcium, silicon, and aluminum in FGDG to form silicate and aluminosilicate minerals. Their formation is the main reason for the increased compressive strength and accelerated coagulation. Full article

In deep coal mining, grouting reinforcement and water blockage are the most effective means for reinforcing the rock mass of extremely broken coal. However, traditional cement grouting materials are not suitable for use in complex strata because of their insufficient early mechanical strength and slow setting time. This study innovatively proposes using alkali-activated grouting material to compensate for the shortcomings of traditional grouting materials and strengthen the reinforcement of extremely unstable broken coal and rock mass. The alkali-activated grouting material was prepared using slag as raw material combined with sodium hydroxide and liquid sodium silicate activation. The compressive strength of specimens cured for 1 d, 3 d, and 28 d was regularly measured and the condensation behavior was analyzed. Using X-ray diffraction and scanning electron microscopy, formation behavior of mineral crystals and microstructure characteristics were further analyzed. The results showed that alkali-activated slag grouting material features prompt and high strength and offers the advantages of rapid setting and adjustable setting time. With an increase in sodium hydroxide content, the compressive strength first increased (maximum increase was 21.1%) and then decreased, while the setting time continued to shorten. With an increase in liquid sodium silicate level, the compressive strength increased significantly (and remained unchanged, maximum increase was 35.9%), while the setting time decreased significantly (and remained unchanged). X-ray diffraction analysis identified the formation of aluminosilicate minerals as the main reason for the excellent mechanical properties and accelerated coagulation rate. Full article

Bovine mastitis is an important disease affecting dairy farming, and it causes large economic losses to the dairy industry. Escherichia coli (E. coli) is considered to be a causative environmental pathogen and frequently enters into mammary glands, causing inflammation. Artemisinin is a highly effective malaria remedy and is not easy to develop drug resistance to. In recent years, other effects of artemisinin (including antitumor, anti-inflammatory, antifungal, etc.) have been increasingly discovered and applied. The current study aimed to investigate whether artemisinin could attenuate E. coli–induced inflammation. Through the E. coli mastitis model in MAC-T cells and mice, the protective effects of artemisinin were analyzed by CCK-8 (Cell Counting Kit-8), Western blot, and RT-qPCR. The results showed that artemisinin reversed the decrease of cell viability and upregulated TLR4 (toll-like receptor 4)/NF-κB (nuclear factor κB) and MAPK (mitogen activated protein kinase)/p38 signaling pathways, as well as restrained the expression of TNF-α, IL-6, and IL-1β mRNA caused by E. coli. Meanwhile, artemisinin also alleviated mammary tissue damage, reduced inflammatory cells’ infiltration, and decreased the levels of inflammatory factors in a mice mastitis model. This study demonstrated that artemisinin alleviated the inflammatory response of mouse mastitis and MAC-T cells induced by E. coli, thus providing a practical approach for the clinical control of mastitis. Full article

Alzheimer’s disease (AD) is pathologically characterized by excessive accumulation of amyloid-beta (Aβ) within extracellular spaces of the brain. Aggregation of Aβ has been shown to trigger oxidative stress, inflammation, and neurotoxicity resulting in cognitive dysfunction. In this study, we use models of cerebral Aβ amyloidosis to investigate anti-amyloidogenic effects of scutellarin in vitro and in vivo. Our results show that scutellarin, through binding to Aβ42, efficiently inhibits oligomerization as well as fibril formation and reduces Aβ oligomer-induced neuronal toxicity in cell line SH-SY5Y. After nine months of treatment in APP/PS1 double-transgenic mice, scutellarin significantly improves behavior, reduces soluble and insoluble Aβ levels in the brain and plasma, decreases Aβ plaque associated gliosis and levels of proinflammatory cytokines TNF-α and IL-6, attenuates neuroinflammation, displays anti-amyloidogenic effects, and highlights the beneficial effects of intervention on development or progression of AD-like neuropathology. Full article