Search Results (99)

As veganism becomes more popular, the number of vegan pregnant women and children is steadily increasing. During vegan pregnancy and early childhood, there is a high risk for nutrient deficiencies that may impair child development. External factors, such as healthcare advice, social networks, and social environments, that affect the diet of vegan pregnant women, parents, and their children, as well as their approach towards dietary supplementation, have not yet been investigated. Various sources of information, combined with a lack of expertise, sparse food and nutritional health literacy, and qualitatively heterogeneous information provision by medical experts, unsettle vegan pregnant women and parents and affect their dietary choices and potentially the health of their children. The VedieS study aims to investigate potential connections between external influences and associated impacts on a vegan diet and the intake of dietary supplements (DS) of pregnant women and children. Two surveys are being conducted within the study: one targeting 1000 vegan pregnant women and parents, and another targeting 60 experts in each of five healthcare groups: gynecologists, pediatricians, general practitioners, pharmacists, and dietitians. This study is the first to examine how socio-economic, social, and further informational factors influence dietary practices during vegan pregnancy and childhood. It highlights the need for reliable, expert-led guidance, as current information sources are often inconsistent and may put these vulnerable groups at risk. Full article

This paper investigates the evolving landscape of Multi-User Conversational Interfaces, addressing the limitations of traditional systems that primarily focus on single-user interactions. As real-world applications grow more complex, there is a pressing need for conversational systems capable of facilitating dialogues among multiple participants. Our systematic survey reviews recent advancements in the field, highlighting innovative architectures, application domains, user and dialogue modelling, and evaluation metrics tailored for multi-user contexts. We conduct a comprehensive analysis of relevant literature, employing both quantitative and qualitative methodologies to identify common patterns and challenges in multi-user interactions. The findings underscore the importance of developing robust interfaces that can effectively manage overlapping dialogues, ensure collaborative group work, and enhance overall conversational quality. This work contributes to the understanding of Multi-User Conversational Interfaces and may help as a basis for future research aiming to develop more natural, user-friendly, and effective conversational interfaces. Full article

Surface soil moisture (SSM) has proven to be an important variable for the yield prediction of main crops like maize and wheat, but its value for spring barley, the third most cultivated crop in Europe, has not yet been evaluated. This study assesses how much of spring barley yield variability can be explained by the commonly used model and satellite-based global SSM products ERA5 SWVL1 and H SAF. A Feed Forward Neural Network, SSM time series, and reference yield data are used to predict spring barley yield at NUTS level for Austria, Czechia, and Germany. A random train-test split is used to assess the explained variability and a cross-validation at the NUTS level for the spatial evaluation. The results indicate the following: (1) ERA5 SWVL1 achieved an R² of 0.37, H SAF an R² of 0.33; (2) Both products achieved the lowest RMSE and MAE in Czechia, high RMSE and MAE values are observed in Eastern Germany. (3) ERA5 SWVL1 performed better in areas with low sensitivity for microwaves like the Alpine region, but both products achieved similar results in 80% of the NUTS regions. These findings contribute to better utilization of SSM and more accurate yield predictions for spring barley and similar crops. Full article

In order to improve the current standard of analysis-ready Synthetic Aperture Radar (SAR) backscatter data, we introduce a machine learning-based approach to estimate the slope of the backscatter–incidence angle relationship from several backscatter statistics. The method requires information from radiometric terrain-corrected gamma nought time series and overcomes the constraints of a limited orbital coverage, as exemplified with the Sentinel-1 constellation. The derived slope estimates contain valuable information on scattering characteristics of different land cover types, allowing for the correction of strong forward-scattering effects over water bodies and wetlands, as well as moderate surface scattering effects over bare soil and sparsely vegetated areas. Comparison of the estimated and computed slope values in areas with adequate orbital coverage shows good overall agreement, with an average RMSE value of 0.1 dB/° and an MAE of 0.05 dB/°. The discrepancy between RMSE and MAE indicates the presence of outliers in the computed slope, which are attributed to speckle and backscatter fluctuations over time. In contrast, the estimated slope excels with a smooth spatial appearance. After correcting backscatter values by normalising them to a certain reference incidence angle, orbital artefacts are significantly reduced. This becomes evident with differences up to 5 dB when aggregating the normalised backscatter measurements over certain time periods to create spatially seamless radar backscatter composites. Without being impacted by systematic differences in the illumination and physical properties of the terrain, these composites constitute a valuable foundation for land cover and land use mapping, as well as bio-geophysical parameter retrieval. Full article

Background and Purpose: Decompressive surgery is a potentially life-saving treatment in patients with malignant space-occupying cerebellar infarction. However, there is only limited literature on functional outcomes and complications after surgery. Our aim was to establish markers which predict poor outcome. Methods: We retrospectively analyzed data of all patients who underwent surgery due to malignant swelling of a space-occupying cerebellar infarction in our hospital between 2005 and 2023. Statistical analyses were conducted on multiple parameters to identify predictors of poor functional outcome (mRS 4–6) 90 days after surgery. Complications during hospitalization were reviewed for each patient. Results: In total, 58 patients received decompressive surgery. The 90-day mortality rate was 27.6% (n = 16). A good functional outcome (mRS 0–3) 90 days after surgery was achieved in 24 patients (41.4%). Multivariable analysis revealed multiple factors associated with a poor outcome on day 90 (mRS 4–6): a higher premorbid mRS score (OR 2.715 [95% CI, 1.166–6.323]; p = 0.021), higher NIHSS score on admission (OR 1.088 [95% CI, 1.014, 1.168]; p = 0.019) and the presence of an additional brainstem infarction (OR 7.035, [95% CI, 1.255, 39.424], p = 0.027). Hyperactive delirium was associated with good clinical outcome (OR 0.020 [95%CI, 0.001–0.623]; p = 0.026). Aspiration pneumonia (n = 22, 37.9%), urinary tract infection (n = 15, 25.9%), and hyperactive delirium (n = 8, 13.8%) were the most common complications during hospitalization. Conclusions: Decompressive surgery is a safe, life-saving treatment for malignant space-occupying cerebellar infarction. Higher premorbid mRS, higher NIHSS score on admission and the presence of brainstem infarction are associated with a poor functional outcome. Full article

Periodontitis is associated with systemic chronic inflammatory diseases. There is limited evidence on the influence of anti-cytokine therapies on the periodontal condition and microbiome in the tooth pocket of such patients, so the aim of this study was to elucidate this issue. In this observational trial, the periodontal status and the gingival crevicular fluid of 13 patients with different chronic inflammatory diseases were obtained before the initiation of anti-cytokine treatment and 14 weeks after. Gingival crevicular fluid was collected for 16S rRNA gene sequencing from a clinically healthy tooth and the deepest measured pocket. The Shannon Diversity Index significantly increased in the deepest pockets of patients (p = 0.039). The data showed alterations in the diversity of the subgingival microbiome over the course of the study, implying a shift towards a healthier condition after starting anti-cytokine therapy. Additional investigations are needed to analyze whether the administration of selective biologicals can improve periodontal conditions in patients with or without chronic inflammatory diseases. Full article

Despite the high prevalence of BK polyomavirus (BKPyV) and the associated risk for BKPyV-associated nephropathy (BKPyVAN) in kidney transplant (KTX) recipients, many details on viral processes such as replication, maturation, assembly and virion release from host cells have not been fully elucidated. VP1 is a polyomavirus-specific protein that is expressed in the late phase of its replicative cycle with important functions in virion assembly and infectious particle release. This study investigated the localization and time-dependent changes in the distribution of VP1-positive viral particles and their association within the spectrum of differing cell morphologies that are observed in the urine of KTX patients upon active BKPyV infection. We found highly differing recognition patterns of two anti-VP1 antibodies with respect to intracellular and extracellular VP1 localization, pointing towards independent binding sites that were seemingly associated with differing stages of virion maturation. Cells originating from single clones were stably cultured out of the urine sediment of KTX recipients with suspected BKPyVAN. The cell morphology, polyploidy, virus replication and protein production were investigated by confocal microscopy using both a monoclonal (mAb 4942) and a polyclonal rabbit anti-VP1-specific antibody (RantiVP1 Ab). Immunoblotting was performed to investigate changes in the VP1 protein. Both antibodies visualized VP1 and the mAb 4942 recognized VP1 in cytoplasmic vesicles exhibiting idiomorphic sizes when released from the cells. In contrast, the polyclonal antibody detected VP1 within the nucleus and in cytoplasm in colocalization with the endoplasmic reticulum marker CNX. At the nuclear rim, VP1 was recognized by both antibodies. Immunoblotting revealed two smaller versions of VP1 in urinary decoy cell extracts, potentially from different translation start sites as evaluated by in silico analysis. Oxford Nanopore sequencing showed integration of BKPyV DNA in chromosomes 3, 4 and 7 in one of the five tested primary cell lines which produced high viral copies throughout four passages before transcending into senescence. The different staining with two VP1-specific antibodies emphasizes the modification of VP1 during the process of virus maturation and cellular exit. The integration of BKPyV into the human genome leads to high virus production; however, this alone does not transform the cell line into a permanently cycling and indefinitely replicating one. Full article

Primary focal segmental glomerulosclerosis (FSGS) is a disease of the podocytes and glomerulus, leading to nephrotic syndrome and progressive loss of renal function. One of the most serious aspects is its recurrence of disease in over 30% of patients following allogeneic kidney transplantation, leading to early graft loss. This research investigates the individual genetic predispositions and differences in the immune responses leading to recurrence of FSGS after transplantation. We performed exome sequencing on six patients with recurrent FSGS to identify variants in fifty-one genes and found significant variations in the alpha-2-macroglobulin (A2M). Immunoblotting was used to investigate effects of specific gene variants at the protein level. Further expression analysis identified A2M, exophilin 5 (EXPH5) and plectin (PLEC) as specific proteins linked to podocytes, endothelial cells, and the glomerulus. Subsequent protein array screening revealed the presence of non-HLA-specific antibodies, including TRIM21, after transplantation. Using Metascape for pathway and process enrichment analysis, we focused on the IL-17 signaling and chemotaxis pathways. ELISA measurements showed significantly elevated IL-17 levels in patients with recurrent FSGS (32.30 ± 9.12 pg/mL) compared to individuals with other glomerular diseases (23.16 ± 2.49 pg/mL; p < 0.01) and healthy subjects (22.28 ± 0.94 pg/mL; p < 0.01), with no significant difference in plasma CCL2/MCP-1 levels between groups. This study explores the molecular dynamics underlying recurrence of FSGS after transplantation, offering insights into potential biomarkers and therapeutic targets for the future development of individualized treatments for transplant patients. Full article

Lactic acid bacteria (LAB) should not only survive, but also perform under increased osmotic pressure in the process of ensiling, which results from the best practice of wilting forage. Simple laboratory protocols are needed to select suitable LAB strains as inoculants for high dry matter (DM) conditions. The aim of this study was to simulate conditions of high osmolality without inducing salt stress and to select a suitable indicator of LAB performance. For that, an MRS medium was enriched with increasing concentrations of glucose and fructose plus a maximum of 28 g KCl/L until achieving an osmolality of 2.4 osmol/kg. Both, growth in the inoculated medium and pH decline, were then compared to the LAB performance in the basic medium. The latter was clearly delayed in the new medium. Finally, the method was validated by comparing the pH of small-scale grass silages of 30–35 and 45–49% target DM after 3–5 days of ensiling to the pH values of the microbiological growth medium. The pH levels of treatments with the homofermentative LAB were clearly attributable to the dry matter or the sugar concentration, respectively. The developed liquid growth medium sufficiently approximates high DM conditions to select for the osmotolerant homofermentative LAB. Full article

To assess changes in treatment modalities for supracondylar humerus fractures (SCHFs) at a large pediatric university hospital, we analyzed patient data from 2014 to 2022. A total of 233 SCHFs treated surgically at our hospital were included. To evaluate postoperative outcome and quality of life, DASH and EuroQol-5D-Y questionnaires were sent to patients. In addition to a significant fluctuation in fracture severity, we found an increase in training interventions (more surgeries were performed by trainees) and a significant decrease in surgery times after 2016. From 2020, there was a significant shift in the type of surgical method away from closed reduction with elastic stable intramedullary nailing (ESIN) and towards closed reduction and crossed K-wire osteosynthesis (CRK). Surgeries performed in the morning and evening hours increased, while those performed in the afternoon and after midnight decreased. After a mean follow-up of 4 years, there was no difference in elbow function between ESIN and open reduction and K-wires (ORK). Treatment with ESIN was equivalent to ORK in terms of function, at least in the medium-term follow-up. In summary, the combination of shifting treatment from SCHF to daytime hours, increasing trainee participation and using cross K-wire fixation instead of ESIN had no negative impact on surgery times. In our setting, these measures have reduced resource utilization and increased efficiency without compromising patient care. Full article

Polyomaviruses are widespread, with BK viruses being most common in humans who require immunosuppression due to allotransplantation. Infection with BK polyomavirus (BKV) may manifest as BK virus-associated nephropathy and hemorrhagic cystitis. Established diagnostic methods include the detection of polyomavirus in urine and blood by PCR and in tissue biopsies via immunohistochemistry. In this study, 79 patients with pathological renal retention parameters and acute kidney injury (AKI) were screened for BK polyomavirus replication by RNA extraction, reverse transcription, and virus-specific qPCR in urine sediment cells. A short fragment of the VP2 coding region was the target of qPCR amplification; patients with (n = 31) and without (n = 48) a history of renal transplantation were included. Urine sediment cell immunofluorescence staining for VP1 BK polyomavirus protein was performed using confocal microscopy. In 22 patients with acute renal injury, urinary sediment cells from 11 participants with kidney transplantation (KTX) and from 11 non-kidney transplanted patients (nonKTX) were positive for BK virus replication. BK virus copies were found more frequently in patients with AKI stage III (n = 14). Higher copy numbers were detected in KTX patients having experienced BK polyoma-nephropathy (BKPyVAN) in the past or diagnosed recently by histology (5.6 × 10⁹–3.1 × 10¹⁰). One patient developed BK viremia following delayed graft function (DGF) with BK virus-positive urine sediment. In nonKTX patients with BK copies, decoy cells were absent; however, positive staining of cells was found with epithelial morphology. Decoy cells were only found in KTX patients with BKPyVAN. In AKI, damage to the tubular epithelium itself may render the epithelial cells more permissive for polyoma replication. This non-invasive diagnostic approach to assess BK polyomavirus replication in urine sediment cells has the potential to identify KTX patients at risk for viremia and BKPyVAN during AKI. This method might serve as a valuable screening tool for close monitoring and tailored immunosuppression decisions. Full article

Patient-derived xenograft (PDX) tumor models are essential for identifying new biomarkers, signaling pathways and novel targets, to better define key factors of therapy response and resistance mechanisms. Therefore, this study aimed at establishing pancreas carcinoma (PC) PDX models with thorough molecular characterization, and the identification of signatures defining responsiveness toward drug treatment. In total, 45 PC-PDXs were generated from 120 patient tumor specimens and the identity of PDX and corresponding patient tumors was validated. The majority of engrafted PDX models represent ductal adenocarcinomas (PDAC). The PDX growth characteristics were assessed, with great variations in doubling times (4 to 32 days). The mutational analyses revealed an individual mutational profile of the PDXs, predominantly showing alterations in the genes encoding KRAS, TP53, FAT1, KMT2D, MUC4, RNF213, ATR, MUC16, GNAS, RANBP2 and CDKN2A. Sensitivity of PDX toward standard of care (SoC) drugs gemcitabine, 5-fluorouracil, oxaliplatin and abraxane, and combinations thereof, revealed PDX models with sensitivity and resistance toward these treatments. We performed correlation analyses of drug sensitivity of these PDX models and their molecular profile to identify signatures for response and resistance. This study strongly supports the importance and value of PDX models for improvement in therapies of PC. Full article

Background: Early detection of acute kidney injury (AKI) is crucial for timely intervention and improved patient outcomes after cardiac surgery. This study aimed to evaluate the potential of urinary collectrin as a novel biomarker for AKI in this patient population. Methods: In this prospective, observational cohort study, 63 patients undergoing elective cardiac surgery with cardiopulmonary bypass (CPB) were studied at the Medical University of Vienna between 2016 and 2018. We collected urine samples prospectively at four perioperative time points, and urinary collectrin was measured using an enzyme-linked immunosorbent assay. Patients were divided into two groups, AKI and non-AKI, defined by Kidney Disease: Improving Global Outcomes Guidelines, and differences between groups were analyzed. Results: Postoperative AKI was found in 19 (30%) patients. Urine sample analysis revealed an inverse correlation between urinary collectrin and creatinine and AKI stages, as well as significant changes in collectrin levels during the perioperative course. Baseline collectrin levels were 5050 ± 3294 pg/mL, decreased after the start of CPB, reached their nadir at the end of surgery, and began to recover slightly on postoperative day (POD) 1. The most effective timepoint for distinguishing between AKI and non-AKI patients based on collectrin levels was POD 1, with collectrin levels of 2190 ± 3728 pg/mL in AKI patients and 3768 ± 3435 pg/mL in non-AKI patients (p = 0.01). Conclusions: Urinary collectrin shows promise as a novel biomarker for the early detection of AKI in patients undergoing cardiac surgery on CPB. Its dynamic changes throughout the perioperative period, especially on POD 1, provide valuable insights for timely diagnosis and intervention. Further research and validation studies are needed to confirm its clinical usefulness and potential impact on patient outcomes. Full article

Sentinel-1-based flood mapping works well but with well-known issues over rugged terrain. Applying exclusion masks to improve the results is common practice in unsupervised and global applications. One such mask is the height above the nearest drainage (HAND), which uses terrain information to reduce flood lookalikes in SAR images. The TU Wien flood mapping algorithm is one operational workflow using this mask. Being a Bayesian method, this algorithm can integrate auxiliary information as prior probabilities to improve classifications. This study improves the TU Wien flood mapping algorithm by introducing a HAND prior function instead of using it as a mask. We estimate the optimal function parameters and observe the performance in flooded and non-flooded scenarios in six study sites. We compare the flood maps generated with HAND and (baseline) non-informed priors with reference CEMS rapid mapping flood extents. Our results show enhanced performance by decreasing false negatives at the cost of slightly increasing false positives. In utilizing a single parametrization, the improved algorithm shows potential for global implementation. Full article

A crucial challenge in Bayesian modeling using Markov chain Monte Carlo (MCMC) estimation is to diagnose the convergence of the chains so that the draws can be expected to closely approximate the posterior distribution on which inference is based. A close approximation guarantees that the MCMC error exhibits only a negligible impact on model estimates and inferences. However, determining whether convergence has been achieved can often be challenging and cumbersome when relying solely on inspecting the trace plots of the chain(s) or manually checking the stopping criteria. In this article, we present a SAS macro called %automcmc that is based on PROC MCMC and that automatically continues to add draws until a user-specified stopping criterion (i.e., a certain potential scale reduction and/or a certain effective sample size) is reached for the chain(s). Full article