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Authors = Paulo César Stringheta

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20 pages, 2737 KiB  
Article
Development of Gelatin/Polyvinyl Alcohol Films Incorporated with Blueberry Extracts for Freshness Detection of Shrimp
by Bárbara Teixeira Gomes, Meirielly Jesus, Joana Santos, Clara Suprani Marques, Noé Mitterhofer Eiterer Ponce de Leon da Costa, Fernando Mata, Paulo Cesar Stringheta, Taila Veloso de Oliveira and Nilda de Fatima Ferreira Soares
Polymers 2025, 17(16), 2188; https://doi.org/10.3390/polym17162188 - 10 Aug 2025
Abstract
The objective of this study was to evaluate the physical, chemical, mechanical, thermal, and topological properties of polyvinyl alcohol (PVA) and gelatin (GL) films after incorporating three different fractions of blueberry extract: crude extract (EB, without purification), phenolic portion (EF), and concentrated anthocyanins [...] Read more.
The objective of this study was to evaluate the physical, chemical, mechanical, thermal, and topological properties of polyvinyl alcohol (PVA) and gelatin (GL) films after incorporating three different fractions of blueberry extract: crude extract (EB, without purification), phenolic portion (EF), and concentrated anthocyanins (EA). Additionally, the study aimed to analyze the efficiency of these colorimetric indicator films in monitoring the freshness quality of shrimp. The experiment followed a completely randomized design with one factor—different types of films—studied at six levels: film incorporated with crude blueberry extract (FB), film incorporated with phenolic extract (FF), and film incorporated with anthocyanin extract (FA), in addition to the control films: the plasticized blend containing glycerol, PVA, and GL (FC), the pristine gelatin film (FG), and the pristine PVA film (FPVA). To evaluate the colorimetric sensitivity of the indicators applied to shrimp, storage time was studied at two levels: T0 (before storage—on the day of collection) and T7 (after 7 days of storage at 6.5 ± 1 °C) for the FB and FA films. Regarding thermal properties, the degradation profile occurred in three stages, with the FC film being the most thermally stable. In terms of mechanical behavior, the isolated anthocyanin content increased the elasticity of FA, while the crude extract and other phenolic compounds contributed to the stiffness of FB (Young’s modulus, YM = 22.52) and FF (YM = 37.33). Structurally, the FC film exhibited a smooth and well-blended polymeric surface, whereas FF, FB, and FA displayed heterogeneous and discontinuous phases. The incorporation of blueberry extracts reduced water absorption, leading to decreased swelling and solubility. FF showed the lowest solubility (S = 16.14%), likely due to hydrogen bonding between phenolic compounds and the polymer matrix. Notably, FB demonstrated superior physical, chemical, and mechanical performance, as well as the highest thermal stability among the extract-containing films. It also showed a visible color change (from purple to green/brown) after 7 days of shrimp storage, corresponding with spoilage and pH values unsuitable for consumption. Both FA and FB effectively monitored shrimp freshness, offering a sustainable approach to quality assurance and food waste reduction. Among them, FB was the most practical for visual detection. Overall, these films demonstrated strong potential as pH-sensitive indicators for evaluating the freshness of shrimp. Full article
(This article belongs to the Section Polymer Membranes and Films)
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30 pages, 449 KiB  
Review
Bioactive Compounds and the Performance of Proteins as Wall Materials for Their Encapsulation
by Therys Senna de Castro Oliveira, Jhonathan Valente Ferreira Gusmão, Thaís Caroline Buttow Rigolon, Daiana Wischral, Pedro Henrique Campelo, Evandro Martins and Paulo Cesar Stringheta
Micro 2025, 5(3), 36; https://doi.org/10.3390/micro5030036 - 31 Jul 2025
Viewed by 343
Abstract
The encapsulation of bioactive compounds using proteins as wall materials has emerged as an effective strategy to enhance their stability, bioavailability, and controlled release. Proteins offer unique functional properties, including amphiphilic behavior, gel-forming ability, and interactions with bioactives, making them ideal candidates for [...] Read more.
The encapsulation of bioactive compounds using proteins as wall materials has emerged as an effective strategy to enhance their stability, bioavailability, and controlled release. Proteins offer unique functional properties, including amphiphilic behavior, gel-forming ability, and interactions with bioactives, making them ideal candidates for encapsulation. Animal-derived proteins, such as whey and casein, exhibit superior performance in stabilizing lipophilic compounds, whereas plant proteins, including soy and pea protein, demonstrate greater affinity for hydrophilic bioactives. Advances in protein modification and the formation of protein–polysaccharide complexes have further improved encapsulation efficiency, particularly for heat- and pH-sensitive compounds. This review explores the physicochemical characteristics of proteins used in encapsulation, the interactions between proteins and bioactives, and the main encapsulation techniques, including spray drying, complex coacervation, nanoemulsions, and electrospinning. Furthermore, the potential applications of encapsulated bioactives in functional foods, pharmaceuticals, and nutraceuticals are discussed, highlighting the role of emerging technologies in optimizing delivery systems. Understanding the synergy between proteins, bioactives, and encapsulation methods is essential for developing more stable, bioavailable, and sustainable functional products. Full article
(This article belongs to the Section Microscale Biology and Medicines)
21 pages, 1223 KiB  
Review
Strategies for Improving the Techno-Functional and Sensory Properties of Bean Protein
by Juliana Eloy Granato Costa, Paula Zambe Azevedo, Jessica da Silva Matos, Daiana Wischral, Thaís Caroline Buttow Rigolon, Paulo César Stringheta, Evandro Martins and Pedro Henrique Campelo
Processes 2025, 13(2), 371; https://doi.org/10.3390/pr13020371 - 29 Jan 2025
Cited by 1 | Viewed by 1095
Abstract
This review aims to understand the techno-functional and structural properties of bean proteins, highlighting their strengths and weaknesses while presenting them as a robust alternative protein source with high potential to become a competitive ingredient in the protein market. For this purpose, ScienceDirect [...] Read more.
This review aims to understand the techno-functional and structural properties of bean proteins, highlighting their strengths and weaknesses while presenting them as a robust alternative protein source with high potential to become a competitive ingredient in the protein market. For this purpose, ScienceDirect and Scopus were used as databases with the keywords “bean proteins”, “protein modifications + beans”, and “techno-functional properties + beans” to consult the relevant literature. This could reduce global dependence on soy and pea proteins. The study compiles various current articles that address desirable techno-functional properties and potential modifications for a wide range of food industry applications. Based on the gathered findings, bean-derived proteins exhibit a more hydrophobic nature and a more compact structure compared to soy and pea proteins. Consequently, they demonstrate superior emulsifying properties and an excellent oil absorption capacity, making them promising ingredients for emulsified products and baked goods. On the other hand, soy and pea proteins perform better in meat-based products and confectionery due to their higher water absorption capacity and good stability. Full article
(This article belongs to the Section Food Process Engineering)
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22 pages, 3967 KiB  
Article
Techno-Functionalities of White Bean Protein Concentrate: A Comparative Study with Soy and Pea Proteins
by Paula Zambe Azevedo, Juliana Eloy Granato Costa, Jessica da Silva Matos, Breno Rodrigues de Souza, Sueli Rodrigues, Fabiano André Narciso Fernandes, Vanelle Maria Silva, Márcia Cristina Teixeira Ribeiro Vidigal, Paulo Cesar Stringheta, Evandro Martins and Pedro Henrique Campelo
Macromol 2025, 5(1), 3; https://doi.org/10.3390/macromol5010003 - 15 Jan 2025
Cited by 1 | Viewed by 2132
Abstract
The study of the techno-functional properties of novel plant-based proteins has gained importance due to their as alternatives to conventional proteins in food systems. This work evaluated the techno-functional and structural properties of white bean protein concentrate (WBPC) in comparison with commercial soy [...] Read more.
The study of the techno-functional properties of novel plant-based proteins has gained importance due to their as alternatives to conventional proteins in food systems. This work evaluated the techno-functional and structural properties of white bean protein concentrate (WBPC) in comparison with commercial soy and pea proteins. The WBPC exhibited a higher foaming capacity (FC) at neutral pH and excellent foam stability (FS) at both tested pH levels, outperforming the commercial proteins. Although the WBPC’s gelation occurred only at concentrations above 16% and its water-holding capacity (WHC) was lower than that of the soy and pea proteins, the WBPC showed a high binding capacity for nonpolar molecules, excelling in its oil-holding capacity (OHC) and forming stable emulsions, which are relevant for stabilization in food products. Additionally, WBPC can form more rigid gel networks, suitable for systems requiring greater mechanical strength. These techno-functional properties indicate that WBPC is a promising alternative source for the plant-based food industry, helping to meet the demand for innovative, sustainable products and contributing to the diversification of protein sources. Full article
(This article belongs to the Collection Advances in Biodegradable Polymers)
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17 pages, 1732 KiB  
Article
Roasted and Unroasted Cocoa Nibs: Bioactive Compounds Analysis and Application in Cereal Bars
by Mariane Sampaio da Silveira de Souza, Larissa Lorrane Rodrigues Borges, Hélia de Barros Kobi, Valdeir Viana Freitas, Thaís Caroline Buttow Rigolon, Lary Souza Olegário, Eliana Alviarez Gutiérrez, Pedro Henrique Campelo Felix, Marcia Cristina Teixeira Ribeiro Vidigal and Paulo Cesar Stringheta
Foods 2024, 13(21), 3510; https://doi.org/10.3390/foods13213510 - 2 Nov 2024
Cited by 3 | Viewed by 2082
Abstract
Cocoa beans contain a variety of nutritional compounds and are rich in biologically active substances. The aim of this study was to utilize cocoa nibs (roasted and unroasted) as the main ingredient in the development of an attractive and convenient product. The produced [...] Read more.
Cocoa beans contain a variety of nutritional compounds and are rich in biologically active substances. The aim of this study was to utilize cocoa nibs (roasted and unroasted) as the main ingredient in the development of an attractive and convenient product. The produced nibs were analyzed for total phenolics, flavonoids, and antioxidant capacity using DPPH, ABTS, and FRAP methods. The primary phenolic compounds and methylxanthines were analyzed by LC/MS. Subsequently, cereal bars were developed, including a control sample (without nibs) and five formulations containing 41% nibs, using various proportions of roasted and unroasted nibs. The influence of the origin of the beans on the product characteristics was also evaluated. The results showed that the roasting process led to a reduction in epicatechin, caffeine, and caffeic acid. Furthermore, the reduction in total phenolics, flavonoids, and antioxidant capacity after roasting was more pronounced in beans from Bahia compared to those from Espírito Santo. Regarding the cereal bars, the results demonstrated that using cocoa from Bahia, the formulation with a higher proportion of unroasted nibs (F80) significantly increased the total phenolic content (1968.85 mg of gallic acid/100 g) and total flavonoids (39.26 mg of quercetin/100 g). This initial study suggests that the use of cocoa nibs as a functional ingredient in cereal bars may be a viable and advantageous option for creating a product with greater antioxidant potential. Full article
(This article belongs to the Section Nutraceuticals, Functional Foods, and Novel Foods)
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13 pages, 446 KiB  
Review
Ohmic Heating in Food Processing: An Overview of Plant-Based Protein Modification
by Israel Felipe dos Santos, Tatiana Colombo Pimentel, Adriano Gomes da Cruz, Paulo César Stringheta, Evandro Martins and Pedro Henrique Campelo
Processes 2024, 12(9), 1800; https://doi.org/10.3390/pr12091800 - 24 Aug 2024
Cited by 5 | Viewed by 4465
Abstract
This review provides an analysis of ohmic heating in food processing and its effect on plant proteins. This study explores the effect of this technology on protein denaturation and aggregation, affecting both non-covalent and covalent bonds. These structural and chemical changes have significant [...] Read more.
This review provides an analysis of ohmic heating in food processing and its effect on plant proteins. This study explores the effect of this technology on protein denaturation and aggregation, affecting both non-covalent and covalent bonds. These structural and chemical changes have significant implications for the techno-functional properties of proteins, contributing to their use in food processing. This article emphasizes the need to adjust processing conditions to maximize the benefits of ohmic heating, distinguishing it from other traditional thermal techniques due to its direct and controllable impact. By highlighting these contributions, this review serves as a resource for researchers and professionals interested in innovation and efficiency in food processing through the use of emerging technologies. Full article
(This article belongs to the Special Issue Feature Papers in the "Food Process Engineering" Section)
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22 pages, 382 KiB  
Review
Nut Proteins as Plant-Based Ingredients: Emerging Ingredients for the Food Industry
by Jessica da Silva Matos, Juliana Eloy Granato Costa, Debora Raquel Gomes Castro Krichanã, Paula Zambe Azevedo, Amanda Lais Alves Almeida Nascimento, Paulo Cesar Stringheta, Evandro Martins and Pedro Henrique Campelo
Processes 2024, 12(8), 1742; https://doi.org/10.3390/pr12081742 - 19 Aug 2024
Cited by 1 | Viewed by 2478
Abstract
This review explores the growing interest in and potential applications of proteins extracted from nuts in the food industry. With an increasing shift toward plant-based diets and sustainable food sources, the nutritional value and functional properties of nut proteins have gained significant attention. [...] Read more.
This review explores the growing interest in and potential applications of proteins extracted from nuts in the food industry. With an increasing shift toward plant-based diets and sustainable food sources, the nutritional value and functional properties of nut proteins have gained significant attention. The composition, digestibility, and bioavailability of these proteins are discussed, emphasizing their role as high-quality substitutes for animal-based proteins. The text further delves into the technological applications of nut proteins, focusing on their ability to stabilize emulsions, enhance texture, and contribute to the development of innovative food products. This review highlights the diverse range of nuts and their unique protein profiles, underscoring the importance of combining different plant protein sources to achieve a well-balanced amino acid composition. As the food industry seeks novel and sustainable protein alternatives, the utilization of nut proteins emerges as a promising avenue with considerable nutritional benefits. Full article
(This article belongs to the Special Issue Feature Papers in the "Food Process Engineering" Section)
16 pages, 2893 KiB  
Article
Design, Optimization, and Modeling Study of Ultrasound-Assisted Extraction of Bioactive Compounds from Purple-Fleshed Sweet Potatoes
by Bárbara Avancini Teixeira, Eliana Alviarez Gutiérrez, Mariane Sampaio da Silveira de Souza, Thaís Caroline Buttow Rigolon, Evandro Martins, Fernando Luiz Pellegrini Pessoa, Márcia Cristina Teixeira Ribeiro Vidigal and Paulo Cesar Stringheta
Foods 2024, 13(10), 1497; https://doi.org/10.3390/foods13101497 - 12 May 2024
Cited by 5 | Viewed by 2036
Abstract
This study focuses on optimizing the ultrasound-assisted extraction (UAE) of bioactive compounds from purple-fleshed sweet potatoes (PFSP) for potential use as natural colorants. Factors such as time, temperature, and solid-to-liquid ratio were varied using a Box–Behnken Design. The optimal conditions were determined as [...] Read more.
This study focuses on optimizing the ultrasound-assisted extraction (UAE) of bioactive compounds from purple-fleshed sweet potatoes (PFSP) for potential use as natural colorants. Factors such as time, temperature, and solid-to-liquid ratio were varied using a Box–Behnken Design. The optimal conditions were determined as 75 min, 70 °C, and a 1:15 m/v solid-to-liquid ratio, resulting in 18.372 mg/100 g total anthocyanin (TA) and 151.160 mg GAE/100 g total phenolic content (TPC). The validation yielded 18.822 mg/100 g for total anthocyanin and 162.174 mg GAE/100 g for total phenolic content, showing a 7% difference from predictions. UAE significantly increased TA extraction by 81% and TPC by 93% compared with the conventional method, with a notable reduction in process time from 24 h to 75 min. Additionally, three kinetic models were tested to compare extraction mechanisms, confirming the efficiency of UAE for PFSP bioactive compound recovery. This study proposes the UAE technique as a highly effective means of extracting bioactive compounds from PFSP, offering promising applications across multiple industries. Full article
(This article belongs to the Section Food Engineering and Technology)
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13 pages, 14225 KiB  
Article
Anthocyanins of Açaí Applied as a Colorimetric Indicator of Milk Spoilage: A Study Using Agar-Agar and Cellulose Acetate as Solid Support to Be Applied in Packaging
by Samiris Côcco Teixeira, Taila Veloso de Oliveira, Lais Fernanda Batista, Rafael Resende Assis Silva, Matheus de Paula Lopes, Alane Rafaela Costa Ribeiro, Thaís Caroline Buttow Rigolon, Paulo César Stringheta and Nilda de Fátima Ferreira Soares
Polysaccharides 2022, 3(4), 715-727; https://doi.org/10.3390/polysaccharides3040041 - 3 Nov 2022
Cited by 8 | Viewed by 3493
Abstract
Food that is still fit for consumption is wasted in the domestic environment every day, so food packaging technologies are being developed that will monitor the quality of the products in real time. Highly perishable milk is currently one of the products that [...] Read more.
Food that is still fit for consumption is wasted in the domestic environment every day, so food packaging technologies are being developed that will monitor the quality of the products in real time. Highly perishable milk is currently one of the products that suffers most from this waste, due to its short shelf life. Active use-by date (AUBD) indicators have been shown to discriminate between fresh and spoiled milk. Colorimetric indicators undergo characteristic changes in their chemical structure, causing abrupt color changes. Among the polymeric materials studied that may function as solid support are cellulose acetate (CA) and agar-agar (AA). The AA colorimetric indicator proved to be more suitable as a solid support due to its ability to maintain the color change properties of the anthocyanin and its high colorimetric performance. The technique was shown to be capable of indicating, in real time, changes in milk quality. Full article
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15 pages, 4416 KiB  
Article
Ionic Strength of Methylcellulose-Based Films: An Alternative for Modulating Mechanical Performance and Hydrophobicity for Potential Food Packaging Application
by Rafael Resende Assis Silva, Clara Suprani Marques, Tarsila Rodrigues Arruda, Samiris Cocco Teixeira, Taíla Veloso de Oliveira, Paulo Cesar Stringheta, Ana Clarissa dos Santos Pires and Nilda de Fátima Ferreira Soares
Polysaccharides 2022, 3(2), 426-440; https://doi.org/10.3390/polysaccharides3020026 - 20 May 2022
Cited by 12 | Viewed by 2918
Abstract
The growing environmental concern with the inappropriate disposal of conventional plastics has driven the development of eco-friendly food packaging. However, the intrinsic characteristics of polymers of a renewable origin, e.g., poor mechanical properties, continue to render their practical application difficult. For this, the [...] Read more.
The growing environmental concern with the inappropriate disposal of conventional plastics has driven the development of eco-friendly food packaging. However, the intrinsic characteristics of polymers of a renewable origin, e.g., poor mechanical properties, continue to render their practical application difficult. For this, the present work studied the influence of ionic strength (IS) from 0 to 500 mM to modulate the physicochemical properties of methylcellulose (MC). Moreover, for protection against biological risks, Nisin-Z was incorporated into MC’s polymeric matrices, providing an active function. The incorporation of salts (LiCl and MgCl2) promoted an increase in the equilibrium moisture content in the polymer matrix, which in turn acted as a plasticizing agent. In this way, films with a hydrophobic surface (98°), high true strain (85%), and low stiffness (1.6 mPa) can be manufactured by addition of salts, modulating the IS to 500 mM. Furthermore, films with an IS of 500 mM, established with LiCl, catalyzed antibacterial activity against E. coli, conferring synergism and extending protection against biological hazards. Therefore, we demonstrated that the IS control of MC dispersion presents a new alternative to achieve films with the synergism of antibacterial activity against Gram-negative bacteria in addition to flexibility, elasticity, and hydrophobicity required in various applications in food packaging. Full article
(This article belongs to the Special Issue Polysaccharides for Application in Packaging)
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11 pages, 255 KiB  
Article
Preparation of Dry Extract of Mikania glomerata Sprengel (Guaco) and Determination of Its Coumarin Levels by Spectrophotometry and HPLC-UV
by Luciana Soares e Silva, Luciane Santos da Silva, Larissa Brumano, Paulo César Stringheta, Miriam Aparecida de Oliveira Pinto, Leticia Oliveira Moreira Dias, Camila De Sá Martins Muller, Elita Scio, Rodrigo Luiz Fabri, Helena C. Castro and Maria Da Penha Henriques do Amaral
Molecules 2012, 17(9), 10344-10354; https://doi.org/10.3390/molecules170910344 - 29 Aug 2012
Cited by 19 | Viewed by 10722
Abstract
Guaco (Mikania glomerata Sprengel) syrup is one of the most popular herbal medicines used to treat the symptoms of asthmatic bronchitis, cough and hoarseness. The coumarin 2H-1-benzopyran-2-one, is one of the major constituents of Guaco and contributes to its pharmacological [...] Read more.
Guaco (Mikania glomerata Sprengel) syrup is one of the most popular herbal medicines used to treat the symptoms of asthmatic bronchitis, cough and hoarseness. The coumarin 2H-1-benzopyran-2-one, is one of the major constituents of Guaco and contributes to its pharmacological effects. The pharmaceutical capsule form of dry extract of Guaco is recommended by the Brazilian Program of Medicinal Plants and Herbal Medicines and used in primary health care. In order to identify a new protocol to obtain the raw material for Guaco capsule production we evaluated two methods, including a freeze-drying process (lyophilization) and the spray-dryer technique, as well as the use of two adjuvants, Maltodextrins and Aerosil®, in different concentrations. The coumarin levels of the dried extracts were analyzed by UV-spectrophotometry and HPLC-UV/DAD. The adjuvant Aerosil® 8% showed better dry powder physical appearance. Lyophilization was observed to be the best process to obtain the dry extract of Guaco based on the measured coumarin levels. Full article
(This article belongs to the Special Issue Coumarins and Xanthones)
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12 pages, 360 KiB  
Article
Pharmacological Proprieties of the Ethanol Extract of Muehlenbeckia platyclada (F. Muell.) Meisn. Leaves
by Leopoldina Leonor Fagundes, Glauciemar Del-Vechio Vieira, José de Jesus R. G. De Pinho, Célia Hitomi Yamamoto, Maria Silvana Alves, Paulo César Stringheta and Orlando Vieira De Sousa
Int. J. Mol. Sci. 2010, 11(10), 3942-3953; https://doi.org/10.3390/ijms11103942 - 15 Oct 2010
Cited by 10 | Viewed by 12135
Abstract
Antinociceptive and anti-inflammatory activities of the Muehlenbeckia platyclada leaves’ ethanol extract were investigated in animal models. The extract (p.o.) reduced the number of abdominal contortions induced by acetic acid by 21.57% (400 mg/kg). After intraplantar injection of formalin, a dose of 400 mg/kg [...] Read more.
Antinociceptive and anti-inflammatory activities of the Muehlenbeckia platyclada leaves’ ethanol extract were investigated in animal models. The extract (p.o.) reduced the number of abdominal contortions induced by acetic acid by 21.57% (400 mg/kg). After intraplantar injection of formalin, a dose of 400 mg/kg (p.o.) inhibited the time spent paw licking in the first phase (26.43%), while the second phase was inhibited by 10.90 and 36.65% at the doses of 200 and 400 mg/kg, respectively. The extract (p.o.) increased the reaction time on a hot plate at a dose of 400 mg/kg (32.68 and 40.30%) after 60 and 90 minutes of treatment, respectively. The paw edema was reduced by extract (p.o.) at doses of 100 (15.46 and 16.67%), 200 (22.68 and 25.64%) and 400 mg/kg (29.50 and 37.33%) after 3 to 4 h of carrageenan application, respectively. Doses of 100, 200 and 400 mg/kg (p.o.), administered 4 h after the carrageenan injection, reduced the exudate volume (11.28, 21.54 and 45.13%), while leukocyte migration was reduced by 21.21 and 29.70% at the doses of 200 and 400 mg/kg, respectively. These results indicate that the ethanol extract from M. platyclada may constitute a potential target for the discovery of new molecules with antinociceptive and anti-inflammatory activities that can be explored for their therapeutic use. Full article
(This article belongs to the Section Biochemistry)
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