Search Results (456)

Sequential drought and heat stress pose a growing threat to forest ecosystems in the context of climate change, yet systematic evaluation methods for woody plants remain limited. This study aimed to develop a comprehensive screening platform for identifying woody plant species tolerant to sequential drought and heat stress among 27 native species growing in Korea. A sequential stress protocol was applied: drought stress for 2 weeks, followed by high-temperature exposure at 45 °C. Physiological indicators, including relative water content (RWC) and electrolyte leakage index (ELI), were used for preliminary screening, supported by phenotypic observations, Evans blue staining for cell death, and DAB staining to assess oxidative stress and recovery ability. The results revealed clear differences among species. Chamaecyparis obtusa, Quercus glauca, and Q. myrsinaefolia exhibited strong tolerance, maintaining high RWC and low ELI values, while Albizia julibrissin was highly susceptible, showing severe membrane damage and low survival. DAB staining successfully distinguished tolerance levels based on oxidative recovery. Additional species such as Camellia sinensis, Q. acuta, Q. phillyraeoides, Q. salicina, and Ternstroemia japonica showed varied responses: Q. phillyraeoides demonstrated high tolerance, T. japonica showed moderate tolerance, and Q. salicina was relatively sensitive. The integrated screening system effectively differentiated tolerant species through multiscale analysis—physiological, cellular, and morphological—demonstrating its robustness and applicability. This study provides a practical and reproducible framework for evaluating sequential drought and heat stress in trees and offers valuable resources for urban forestry, reforestation, and climate-resilient species selection. Full article

Background: Cold feet syndrome is characterized by hypersensitivity of sympathetic nerves to cold stimuli, resulting in vasoconstriction and reduced peripheral blood flow. This condition causes an intense cold sensation, particularly in the extremities. Although hormonal changes (e.g., during childbirth or menopause) and psychological stress have been implicated, the mechanisms and effective treatments remain unclear. Methods: Ninety adult volunteers were randomized into three groups based on the type of heating mat applied to the feet, with surface temperatures gradually increased from 20 °C to 50 °C. Group A (control) used non-FIR electric mats, Group B used carbon FIR mats, and Group C used loess bio-ball FIR mats. Blood flow (mL/min/100 g) and epidermal temperature (°C) in the left big toe (LBT) and right big toe (RBT) were measured before and after heating or FIR exposure using laser Doppler flowmetry and infrared thermometers. Results: No significant changes in blood flow or skin temperature were observed in Group A. In Group B, blood flow increased by 15.07 mL/min/100 g in the LBT (from 4.12 ± 2.22 to 19.19 ± 5.44) and by 14.55 mL/min/100 g in the RBT (from 4.26 ± 2.29 to 18.81 ± 4.29). In Group C, blood flow increased by 32.86 mL/min/100 g in the LBT (from 4.23 ± 1.64 to 37.09 ± 6.04) and by 32.63 mL/min/100 g in the RBT (from 4.20 ± 1.61 to 36.83 ± 6.48). Epidermal temperature also increased significantly in Group C. All changes in Groups B and C were statistically significant (p < 0.05), with Group C showing the most prominent enhancement. Conclusions: The loess bio-ball mat significantly increased both peripheral blood flow and epidermal temperature compared to the electric and carbon mats. These findings suggest that FIR emitted from loess bio-balls may enhance peripheral circulation through hypothalamus thermogenic response and nitric oxide (NO)-dependent pathways and could serve as a complementary and non-invasive intervention for individuals with poor blood flow. Full article

Background/Objectives: This study aimed to evaluate digital markers and establish quantitative diagnostic criteria for spinal malpositions in Chuna manual therapy using lumbar X-rays. Methods: A total of 2000 X-ray images were collected from adult patients at the International St. Mary’s Hospital of Catholic Kwandong University. Five Chuna manual medicine experts annotated anatomical landmarks using a digital marker labeling program and diagnosed three types of spinal malpositions: flexion/extension, lateral bending, and rotation. Diagnostic accuracy was evaluated using weighted F1 (F1_W) scores, and the optimal threshold values for each malposition type were determined based on maximum F1_W performance. Results: The results showed high diagnostic performance, with average maximum F1_W scores of 0.76 for flexion/extension, 0.85 for lateral bending, and 0.71 for rotation. Based on this analysis, threshold angles for each type of spinal malposition in Chuna manual diagnosis were determined. Conclusions: This study demonstrates the diagnostic validity of digital marker-based X-ray analysis in Chuna manual therapy and is the first to propose quantitative diagnostic thresholds for spinal malpositions. These findings may serve as a foundation for clinical application in spinal assessment and treatment planning, with further validation studies warranted. Full article

Probiotics have recently gained attention as modulators of the gut–brain axis in neurodegenerative diseases such as Alzheimer’s disease. In this study, we identified probiotic strains with neuroprotective effects and investigated their mechanisms and safety. We screened strains based on their ability to inhibit acetylcholinesterase (AChE) activity and protect cells against H₂O₂-induced damage. The cell-free supernatants (CFS) of Lactobacillus gasseri MG4247 and Lacticaseibacillus rhamnosus MG4644 inhibited AChE activity and reduced cell damage and reactive oxygen species generation. These effects were mediated through inhibition of the MyD88/NF-κB pathway and modulation of the JNK/Bax-dependent apoptotic pathway in neuronal cells treated with H₂O₂. Whole-genome sequencing and antibiotic susceptibility testing confirmed the identity and safety of both strains. These findings suggest that MG4247 and MG4644, as probiotics, may help protect neuronal cells from oxidative stress and inflammation. Full article

Bioinspired morphing offers a powerful route to higher aerodynamic and hydrodynamic efficiency. Birds reposition feathers, bats extend compliant membrane wings, and fish modulate fin stiffness, tailoring lift, drag, and thrust in real time. To capture these advantages, engineers are developing airfoils, rotor blades, and hydrofoils that actively change shape, reducing drag, improving maneuverability, and harvesting energy from unsteady flows. This review surveys over 296 studies, with primary emphasis on literature published between 2015 and 2025, distilling four biological archetypes—avian wing morphing, bat-wing elasticity, fish-fin compliance, and tubercled marine flippers—and tracing their translation into morphing aircraft, ornithopters, rotorcraft, unmanned aerial vehicles, and tidal or wave-energy converters. We compare experimental demonstrations and numerical simulations, identify consensus performance gains (up to 30% increase in lift-to-drag ratio, 4 dB noise reduction, and 15% boost in propulsive or power-capture efficiency), and analyze materials, actuation, control strategies, certification, and durability as the main barriers to deployment. Advances in multifunctional composites, electroactive polymers, and model-based adaptive control have moved prototypes from laboratory proof-of-concept toward field testing. Continued collaboration among biology, materials science, control engineering, and fluid dynamics is essential to unlock robust, scalable morphing technologies that meet future efficiency and sustainability targets. Full article

Background: This retrospective, multi-reader, blinded, pivotal trial assessed the performance of artificial intelligence (AI)-based clinical decision support system used to improve the clinician detection of emergent large vessel occlusion (ELVO) using brain non-contrast computed tomography (NCCT) images. Methods: We enrolled 477 patients, of which 112 had anterior circulation ELVO, and 365 served as controls. First, patients were evaluated by the consensus of four clinicians without AI assistance through the identification of ELVO using NCCT images. After a 2-week washout period, the same investigators performed an AI-assisted evaluation. The primary and secondary endpoints in ELVO prediction between unassisted and assisted readings were sensitivity and specificity and AUROC and individual-level sensitivity and specificity, respectively. The standalone predictive ability of the AI system was also analyzed. Results: The assisted evaluations resulted in higher sensitivity and specificity than the unassisted evaluations at 75.9% vs. 92.0% (p < 0.01) and 83.0% vs. 92.6% (p < 0.01) while also resulting in higher accuracy and AUROC at 81.3% vs. 92.5%, (p < 0.01) and 0.87 [95% CI: 0.84–0.90] vs. 0.95 [95% CI: 0.93–0.97] (p < 0.01). Furthermore, the AI system improved sensitivity and specificity for three and four readers, respectively, and had a standalone sensitivity of 88.4% (95% CI: 81.0–93.7) and a specificity of 91.2% (95% CI: 87.9–93.9). Conclusions: This study shows that an AI-based clinical decision support system can improve the clinical detection of ELVO using NCCT. Moreover, the AI system may facilitate acute stroke reperfusion therapy by assisting physicians in the initial triaging of patients, particularly in thrombectomy-incapable centers. Full article

Background: Far infrared rays (FIR) can promote microcirculation of blood in capillaries and reduce inflammation and edema by improving lymphatic circulation. Although the short-term therapeutic use of FIR is increasingly common in clinical settings, its effects on inflammatory fluids during sleep are not yet well established. Methods: This was a small-scale exploratory study conducted on patients exhibiting early symptoms of edema or swelling, or participants who reported discomfort possibly due to such symptoms. Changes in impedance parameters related to inflammatory body fluids were measured in subjects (n = 55) lying lay on either a conventional electric mat (non-FIR) or a loess bio-ball mat (FIR) set at 40 °C for 30 min. To assess the effects of heat and FIR exposure during sleep, additional impedance measurements were taken in subjects (n = 60) who used either on an electric mat or a loess bio-ball mat set at 30 °C during sleep. Results: A total of 115 participants were included in four experimental conditions. In subjects exposed to conductive heat or FIR for 30 min while lying on an electric mat and a loess bio-ball mat set at 40 °C, only minimal changes were observed in impedance parameters and inflammatory fluid-related values. However, significant changes were seen in subjects (n = 30) who slept for 7 h on a loess bio-ball mat set at 30 °C. These changes are presumed to results from the deeper sleep and FIR emitted from the loess bio-balls. In contrast, no significant changes were observed in the group (n = 30) that used the electric mat at 30 °C under the same sleep conditions. Conclusions: Sleeping on a FIR-emitting loess bio-ball mat may stimulate biological tissues and lymphatic circulation, gradually reducing inflammatory fluid accumulation. This suggest potential benefits for improving the physiological condition and function of cell membranes and muscles. Full article

Exercise-induced hypertension (EIH) has increasingly been observed among middle-aged long-distance runners, raising concerns about cardiovascular risk. This study aimed to investigate acute changes in cardiovascular biomarkers associated with vascular inflammation, oxidative stress, antioxidant defense, endothelial function, and myocardial burden in runners with EIH. Thirty-seven middle-aged male runners (aged 40–65 years) were categorized into a normal blood pressure group (NBPG; systolic blood pressure <210 mmHg, n = 23) and an EIH group (EIHG; ≥210 mmHg, n = 14) based on maximal systolic blood pressure during a graded exercise test (GXT). Participants performed a 30 min treadmill run at 80% heart rate reserve, and blood samples were collected before and after exercise. The biomarkers analyzed included high-sensitivity C-reactive protein (hs-CRP), derivatives of reactive oxygen metabolites (d-ROMs), biological antioxidant potential (BAP), nitric oxide (NO), superoxide dismutase (SOD), and N-terminal pro-brain natriuretic peptide (NT-proBNP). The results show that the EIHG exhibited increased NT-proBNP and SOD levels, along with a reduced NO response, indicating elevated myocardial stress and impaired vasodilation. hs-CRP was positively correlated with multiple hemodynamic indices, and SOD levels were associated with maximal systolic pressure and myocardial burden. These findings highlight the need for individualized monitoring and cardiovascular risk management in runners with EIH. Full article

Fabry disease (FD) is a lysosomal storage disorder caused by pathogenic variants in the gene encoding alpha-galactosidase A (GLA). Deficiency of GLA results in the progressive accumulation of glycosphingolipids in virtually all organs, resulting in a progressive multisystem disease. Due to multi-organ involvement in FD, a comprehensive, multidisciplinary approach to diagnosis and treatment with regular follow-ups is essential. The Pusan National University Yangsan Hospital (PNUYH) multidisciplinary care model of FD aims to provide detailed practice guidelines and evidence-based recommendations for the diagnosis, screening, and treatment of FD according to specialty. This guideline focuses on the “quarterback” type of multidisciplinary team (MDT) operation and is limited in its applicability to the Korean insurance system. However, it reflects our team’s extensive experience and insights into optimizing MDT operations within these constraints and is expected to be highly beneficial for centers initiating MDTs for the effective treatment of FD. Full article

Background/Objectives: This Phase 3, randomised, double-blind, multicentre trial evaluated the efficacy and safety of a novel hyaluronic acid hydrogel cross-linked with hexamethylenediamine (HMDA-HA) compared to a conventional 1,4-butanediol diglycidyl ether cross-linked HA (BDDE-HA) in patients with mild-to-moderate knee osteoarthritis (OA). Methods: A total of 223 adults (mean age 63.5 years; 167 women) with Kellgren–Lawrence (KL) grade I–III knee OA were randomised 1:1 to receive two intra-articular injections of HMDA-HA or BDDE-HA at baseline and at 24 weeks. The primary endpoint was changes from baseline in weight-bearing pain (WBP) on a 100 mm visual analogue scale (VAS) at Week 12, assessed in the per-protocol population. A non-inferiority margin of 10 mm was predefined. Secondary outcomes included global assessments, Western Ontario and McMaster Universities Osteoarthritis (WOMAC) index scores, responder rates, and rescue medication use [ClinicalTrials.gov: NCT06307847]. Results: At Week 12, least squares mean change (standard error [SE]) in WBP was −23.72 (1.88) mm in the HMDA-HA group (n = 83) and −25.99 (1.76) mm in the BDDE-HA group (n = 95), yielding a difference of 2.26 mm (95% confidence interval [CI]: −2.83 to 7.34; p = 0.3825), thus demonstrating the non-inferiority of HMDA-HA to BDDE-HA. Secondary outcomes were comparable between groups. A total of 136 adverse events were reported: 44 (41.1%) in the HMDA-HA group and 32 (28.1%) in the BDDE-HA group, with no treatment-related adverse drug reactions. Conclusions: A single-injection intra-articular regimen of HMDA-HA was effective and safe for the treatment of adult patients with mild-to-moderate knee OA. Full article

Influenza A virus (IAV) poses significant public health challenges due to its rapid mutation and drug resistance, necessitating novel antiviral strategies. Rhododendron brachycarpum, traditionally employed in folk medicine to treat inflammatory conditions, contains bioactive flavonoids with potential antiviral effects. In this study, we investigated the anti-influenza activity of R. brachycarpum leaf extract and identified hyperoside (quercetin-3-O-galactoside) as the active constituent. The crude extract and its n-butanol fraction markedly reduced IAV replication in Madin–Darby canine kidney (MDCK) cells, with IC50/CC50 values of 74.51/201.09 μg/mL and 24.5/113.1 μg/mL, respectively. Hyperoside, purified via bioactivity-guided fractionation and HPLC analysis, demonstrated potent antiviral activity, with an IC50 of 66.59 μM (30.92 μg/mL) and a CC50 of 318.9 μM (148.1 μg/mL), indicating a favorable selectivity index. It significantly suppressed viral mRNA and protein expression in infected cells. Time-of-addition and hemagglutination inhibition assays suggested that hyperoside exerts antiviral effects during early infection stages, likely interfering with viral entry. In silico molecular docking analysis further supported this mechanism, revealing that hyperoside binds strongly to the receptor-binding domain of hemagglutinin (−11.5 kcal/mol), potentially blocking viral attachment. These findings reveal that hyperoside is a major antiviral component of R. brachycarpum and underscore its therapeutic potential as a natural antiviral candidate against IAV infections. Full article

The fried potato market is a high-value industry, exceeding USD 55 billion and still growing. However, the genetic mechanisms underlying key frying traits remain poorly understood. In this study, we conducted a transcriptome analysis on two types of potatoes with distinct end-use purposes to identify the gene expression profiles related to desirable frying qualities, focusing on texture and appearance after frying. Key genes encoding starch synthase 1 and 3, sucrose synthase 4, invertases, and pectin methyl-esterase inhibitors were found to be differentially regulated in waxy and mealy potatoes based on their frying characteristics. Notably, mealy potatoes exhibited a higher expression of starch synthesis-related genes and a lower expression of invertase genes. These expression patterns may enhance glucose-to-starch conversion, thereby reducing glucose levels and minimizing sugar-induced browning, which results in a lighter fried appearance. Additionally, we identified two transcription factors, StbZIP2 and StbZIP35, that are potentially co-expressed with two starch synthases. These transcription factors are responsive to abscisic acid, a key hormonal regulator involved in tuber development. This study provides transcriptomic insights for processing quality improvement and identifies key candidate genes for marker-assisted breeding. Further studies across more diverse samples with integrative multi-omics approaches will strengthen the application of these insights in breeding programs. Full article

Conventional diagnostic methods (CDMs) for lower respiratory infections (LRIs) have limitations in detecting causative pathogens. This study evaluates the utility of shotgun metagenomic sequencing (SMS) as a complementary diagnostic tool using bronchoalveolar lavage (BAL) fluid. Sixteen BAL fluid samples from pneumonia patients with positive CDM results—including bacterial/fungal cultures; PCR for Mycobacterium tuberculosis or cytomegalovirus; and the BioFire^® FilmArray^® Pneumonia Panel (BioFire Diagnostics LLC, Salt Lake City, UT, USA)—underwent 10 Gb SMS on the Illumina NovaSeq 6000 platform (Illumina, San Diego, CA, USA). Reads were aligned to the NCBI RefSeq database; with fungal identification further supported by internal transcribed spacer (ITS) analysis. Antibiotic resistance genes (ARGs) were annotated using the Comprehensive Antibiotic Resistance Database. Microbial reads accounted for 0.00002–0.04971% per sample. SMS detected corresponding bacteria in 63% of cases, increasing to 69% when subdominant taxa were included. Fungal reads were low; however, Candida species were identified in four samples via ITS. No viral reads were detected. ARGs meeting perfect match criteria were found in two cases. This is the first real-world study comparing SMS with CDMs, including semiquantitative PCR, in BAL fluid for LRI. SMS shows promise as a supplementary diagnostic method, with further research needed to optimize its performance and cost-effectiveness. Full article

Background: Helicobacter pylori (HP) and Epstein–Barr virus (EBV) coinfection lead to chronic inflammation and contribute to the development of gastric cancer. However, studies examining the association between HP virulence factors and EBV infection in gastric cancer are limited. This study investigated the polymorphisms of HP virulence factors associated with EBV infection and their effects on clinical outcomes in EBV-associated gastric cancer (EBVaGC). Methods: A total of 96 HP isolates from 54 patients with gastric cancer were divided and analyzed based on EBV coinfection status. Polymerase chain reaction amplifications of virulence factors were conducted using DNA extracts from HP isolates cultured from gastric mucosal specimens. Results: EBV infection was significantly associated with gastric carcinoma with lymphoid stroma morphology and a proximal location in the stomach. Most HP strains from patients with gastric cancer were positive for cagA (100.0%), vacA (100.0%), and iceA1 (87.5%). Among HP isolates with EBV coinfection, the prevalence of iceA2 (21.7% vs. 0.0%, p < 0.001) and ureA (21.7% vs. 4.0%, p = 0.009) was significantly more frequent, and that of iceA1 (78.3% vs. 96.0%, p = 0.009) and vacA s1a (4.3% vs. 22.0%, p = 0.012) was less frequent than those of EBV– colonies. Multivariate analysis indicated that ureA (odds ratio, 6.148; 95% confidence interval [CI], 1.221 to 30.958; p = 0.028) was associated with EBVaGC. No significant difference in clinical outcomes was observed based on the presence of ureA expression in EBVaGC. Conclusions: In gastric cancer, regardless of EBV infection, most HP strains were highly virulent, testing positive for cagA, vacA, and iceA1. Although ureA was significantly associated with EBV infection, it did not influence the clinical outcomes of EBVaGC. Full article

This study aims to improve firestop application standards by experimentally analyzing changes in fire-resistance performance due to variations in opening size, penetrant diameter, and firestop material volume. Fire-resistance tests were conducted for 120 min in accordance with KS F ISO 10295-1, using eight specimens with systematically varied cross-sectional areas of openings and penetrants. For nonmetallic pipes, which soften or melt at elevated temperatures, the results show that the completeness of the opening seal and the amount of firestop material are the primary factors governing fire resistance. When the firestop-to-opening area ratio decreased from 95–91%, the maximum temperature on the unexposed surface was, on average, 15–30 °C lower, confirming that modest reductions in firestop material volume for small openings do not compromise performance. In contrast, metallic pipes retained structural integrity and acted as direct heat-transfer paths; fire-resistance performance was more strongly influenced by penetrant diameter and cross-sectional area than by opening size. These findings provide quantitative evidence to support flexible design criteria for firestop systems and offer a practical basis for transitioning toward performance-based approval standards. Full article