Search Results (22)

Background/Objectives: Hepatocellular carcinoma (HCC) is a major cause of cancer-related mortality and often develops in the context of liver cirrhosis (LC). Its detection remains a clinical challenge, particularly with limited sensitivity of the current serum biomarkers and qualitative imaging tools. The aim of this pilot study is to evaluate the application of a combined model based on the use of Liver to Spleen Volume Ratio (LSVR), a score of regional liver remodeling, and Liver Vein to Cava Attenuation (LVCA), a computed tomography (CT)-based perfusion-related parameter, to diagnose HCC in patients with LC. Methods: In this observational retrospective pilot study, 36 patients with LC, with or without HCC, were enrolled from a single tertiary care center between 2021 and 2024. Demographic, clinical, biochemical, and imaging data were collected. LSVR and LVCA were calculated from contrast-enhanced CT scans. Predictors of HCC were assessed using conditional inference trees and multivariate logistic regression. Model performance was evaluated using the area under the receiver operating characteristic curve (AUC). A p-value < 0.05 was considered statistically significant. Results: LVCA and LSVR levels were significantly higher in the HCC group (p < 0.001). In multivariate analysis, LVCA was significantly associated with HCC onset (Odds Ratio = 2.88, p = 0.0075). The final model incorporating both LVCA and LSVR achieved excellent discrimination (AUC = 0.967), with 91% sensitivity and 88% specificity. The combined model outperformed LSVR alone (p = 0.030), though not LVCA alone. Conclusions: Our pilot study suggests the utility of LVCA and LSVR as potential non-invasive imaging tools for HCC diagnosis. External validation in multicenter cohorts and longitudinal studies assessing the temporal evolution of LSVR and LVCA are necessary to better evaluate their application in clinical practice. Full article

The SIDDHARTA-2 collaboration has developed a novel X-ray detection system based on cadmium-zinc-telluride (CZT, CdZnTe), marking the first application of this technology at the DA$\Phi$NE electron-positron collider at INFN-LNF. This work aims to demonstrate the stability of the detectors’ performance in terms of linearity and resolution over short and long periods, thereby establishing their suitability for precise spectroscopic measurements within a collider environment. A reference calibration spectrum is presented in association with findings from assessments of linearity and resolution stability. Additionally, this study introduces a validated model of the response function of the detector. The relative deviations from the nominal values for the source transitions, obtained by fitting the entire spectrum with a background function and the previously introduced response function, are reported. Finally, a comparison of the calibration performance with and without beams circulating in the collider’s rings is presented. These promising results pave the way for applying CZT detectors in kaonic atom studies and, more generally, in particle and nuclear physics spectroscopy. Full article

The Pauli exclusion principle (PEP), a cornerstone of quantum mechanics and whole science, states that in a system, two fermions can not simultaneously occupy the same quantum state. Several experimental tests have been performed to place increasingly stringent bounds on the validity of PEP. Among these, the series of VIP experiments, performed at the Gran Sasso Underground National Laboratory of INFN, is searching for PEP-violating atomic X-ray transitions in copper. In this paper, the upgraded VIP-3 setup is described, designed to extend these investigations to higher-Z elements such as zirconium, silver, palladium, and tin. We detail the enhanced design of this setup, including the implementation of cutting-edge, 1 mm thick, silicon drift detectors, which significantly improve the measurement sensitivity at higher energies. Additionally, we present calculations of expected PEP-violating energy shifts in the characteristic lines of these elements, performed using the multi-configurational Dirac–Fock method from first principles. The VIP-3 realization will contribute to ongoing research into PEP violation for different elements, offering new insights and directions for future studies. Full article

Background: Acute respiratory distress syndrome (ARDS) presents a significant challenge in critical care settings, characterized by compromised gas exchange, necessitating in the most severe cases interventions such as veno-venous extracorporeal membrane oxygenation (vv-ECMO) when conventional therapies fail. Critically ill ARDS patients on vv-ECMO may experience several complications. Limited data exist comparing complication rates between COVID-19 and non-COVID-19 ARDS patients undergoing vv-ECMO. This retrospective observational study aimed to assess and compare complications in these patient cohorts. Methods: We retrospectively analyzed the medical records of all patients receiving vv-ECMO for ARDS between March 2020 and March 2022. We recorded the baseline characteristics, the disease course and complication (barotrauma, bleeding, thrombosis) before and after ECMO cannulation, and clinical outcomes (mechanical ventilation and ECMO duration, intensive care unit, and hospital lengths of stay and mortalities). Data were compared between COVID-19 and non-COVID-19 patients. In addition, we compared survived and deceased patients. Results: Sixty-four patients were included. COVID-19 patients (n = 25) showed higher rates of pneumothorax (28% vs. 8%, p = 0.039) with subcutaneous emphysema (24% vs. 5%, p = 0.048) and longer non-invasive ventilation duration before vv-ECMO cannulation (2 [1; 4] vs. 0 [0; 1] days, p = <0.001), compared to non-COVID-19 patients (n = 39). However, complication rates and clinical outcomes post-vv-ECMO were similar between groups. Survival analysis revealed no significant differences in pre-vv-ECMO complications, but non-surviving patients had a trend toward higher complication rates and more pleural effusions post-vv-ECMO. Conclusions: COVID-19 patients on vv-ECMO exhibit higher pneumothorax rates with subcutaneous emphysema pre-cannulation; post-cannulation complications are comparable to non-COVID-19 patients. Full article

Proton beam therapy is considered a step forward with respect to electromagnetic radiation, thanks to the reduction in the dose delivered. Among unwanted effects to healthy tissue, cardiovascular complications are a known long-term radiotherapy complication. The transcriptional response of cardiac tissue from xenografted BALB/c nude mice obtained at 3 and 10 days after proton irradiation covering both the tumor region and the underlying healthy tissue was analyzed as a function of dose and time. Three doses were used: 2 Gy, 6 Gy, and 9 Gy. The intermediate dose had caused the greatest impact at 3 days after irradiation: at 2 Gy, 219 genes were differently expressed, many of them represented by zinc finger proteins; at 6 Gy, there were 1109, with a predominance of genes involved in energy metabolism and responses to stimuli; and at 9 Gy, there were 105, mainly represented by zinc finger proteins and molecules involved in the regulation of cardiac function. After 10 days, no significant effects were detected, suggesting that cellular repair mechanisms had defused the potential alterations in gene expression. The nonlinear dose–response curve indicates a need to update the models built on photons to improve accuracy in health risk prediction. Our data also suggest a possible role for zinc finger protein genes as markers of proton therapy efficacy. Full article

In this work, we report on tests performed with an experimental apparatus prototype based on a broad-energy germanium detector aimed at investigating topical, foundational issues in quantum mechanics: i.e., possible violations of the spin-statistics connection and models of dynamical wave function collapse. Our recent phenomenological analyses demonstrated the importance of pushing the research of new physics signal, predicted in these fields, to an energy range below 10 keV. We describe the development of the dedicated data acquisition system and of the pulse shape discrimination algorithm, which have already allowed us to get a factor two improvement in the lower energy threshold. Future plans are discussed to further improve the lower energy threshold to the level of a few keV. Full article

The aim of the SIDDHARTA-2 experiment is to perform the first measurement ever of the width and shift induced by the strong interaction to the $2p\to 1s$ energy transition of kaonic deuterium. This ambitious goal implies a challenging task due to the very low X-ray yield of kaonic deuterium, which is why an accurate and thorough characterization of the experimental apparatus is mandatory before starting the data-taking campaign. Helium-4 is an excellent candidate for this characterization since it exhibits a high yield in particular for the $3d\to 2p$ transition, roughly 100 times greater than that of the kaonic deuterium. The ultimate goal of the work reported in this paper is to study the performances of the full experimental setup in view of the kaonic deuterium measurement. This is carried out by measuring the values of the shift and the width for the $3d\to 2p$ energy transition of kaonic helium-4, induced by the strong interaction. The values obtained for these quantities, for a total integrated luminosity of ∼31/$\mathrm{p}$$\mathrm{b}$, are ${\epsilon }_{2p}=2.0\pm 1.2\left(\mathrm{stat}\right)\pm 1.5\left(\mathrm{syst}\right)\mathrm{eV}$ and ${\mathrm{\Gamma }}_{2p}=1.9\pm 5.7\left(\mathrm{stat}\right)\pm 0.7\left(\mathrm{syst}\right)\mathrm{eV}$. The results, compared to the value of the shift measured by the SIDDHARTA experiment ${\epsilon }_{2p}=0\pm 6\left(\mathrm{stat}\right)\pm 2\left(\mathrm{syst}\right)\mathrm{eV}$, show a net enhancement of the resolution of the apparatus, providing strong evidence of the potential to perform the challenging measurement of the kaonic deuterium. Full article

Background: The COVID-19 pandemic has had a significant impact on the epidemiology of respiratory viruses. Non-pharmaceutical interventions (NPIs) led to a dramatic reduction in respiratory infections. However, the long-term effects on respiratory virus epidemiology remain unclear. Materials and Methods: We conducted a comparative study on hospitalized pediatric patients with respiratory illness during two seasons: 1 October 2021 to 15 March 2022 and 1 October 2022 to 15 March 2023. We compared the type of virus, mean duration of hospitalization, and disease severity. Results: In the first season, 47.1% of patients (65/138) tested positive for at least one respiratory virus, with respiratory syncytial virus (RSV) being the most frequent (23.2%). In the second season, 82.9% of patients (102/123) tested positive, with RSV and Rhinovirus being the most prevalent (28.38% and 27.03%, respectively). Other viruses, such as Influenza A/B, Metapneumovirus, and Adenovirus, also showed increased prevalence. Disease severity and mean duration of hospitalization were similar between the two seasons. Conclusions: Our study highlights increased prevalence in respiratory viruses, including RSV and Rhinovirus, following the easing of NPIs. The prevalence in respiratory viruses, including RSV and Rhinovirus, increased in the second season compared to the first one. Interestingly, RSV’s peak incidence shifted from February to November. The emergence of rhinovirus as the most prevalent respiratory virus during certain months suggests viral competition and dynamic changes in viral circulation. The overall severity of respiratory infections remained relatively stable between the seasons. Full article

Kaonic atom X-ray spectroscopy is a consolidated technique for investigations on the physics of strong kaon–nucleus/nucleon interaction. Several experiments have been conducted regarding the measurement of soft X-ray emission (<20 keV) from light kaonic atoms (hydrogen, deuterium, and helium). Currently, there have been new research activities within the framework of the SIDDHARTA-2 experiment and EXCALIBUR proposal focusing on performing precise and accurate measurements of hard X-rays (>20 keV) from intermediate kaonic atoms (carbon, aluminum, and sulfur). In this context, we investigated cadmium–zinc–telluride (CdZnTe or CZT) detectors, which have recently demonstrated high-resolution capabilities for hard X-ray and gamma-ray detection. A demonstrator prototype based on a new cadmium–zinc–telluride quasi-hemispherical detector and custom digital pulse processing electronics was developed. The detector covered a detection area of 1 cm² with a single readout channel and interesting room-temperature performance with energy resolution of 4.4% (2.6 keV), 3% (3.7 keV), and 1.4% (9.3 keV) FWHM at 59.5, 122.1, and 662 keV, respectively. The results from X-ray measurements at the DAΦNE collider at the INFN National Laboratories of Frascati (Italy) are also presented with particular attention to the effects and rejection of electromagnetic and hadronic background. Full article

This study is aimed to set severe constraints on a whole class of non-commutative space-times scenarios as a class of universality for several quantum gravity models. To this end, slight violations of the Pauli exclusion principle—predicted by these models—are investigated by searching for Pauli forbidden K${}_{\alpha }$ and K${}_{\beta }$ transitions in lead. The selection of a high atomic number target material allows to test the energy scale of the space-time non-commutativity emergence at high atomic transition energies. As a consequence, the measurement is very sensitive to high orders in the power series expansion of the Pauli violation probability, which allows to set the first constraint to the “triply special relativity” model proposed by Kowalski-Glikman and Smolin. The characteristic energy scale of the model is bound to $\Lambda >5.6·{10}^{-9}$ Planck scales. Full article

Modern physics lays its foundations on the pillars of Quantum Mechanics (QM), which has been proven successful to describe the microscopic world of atoms and particles, leading to the construction of the Standard Model. Despite the big success, the old open questions at its very heart, such as the measurement problem and the wave function collapse, are still open. Various theories consider scenarios which could encompass a departure from the predictions of the standard QM, such as extra-dimensions or deformations of the Lorentz/Poincaré symmetries. At the Italian National Gran Sasso underground Laboratory LNGS, we search for evidence of new physics proceeding from models beyond standard QM, using radiation detectors. Collapse models addressing the foundations of QM, such as the gravity-related Diósi–Penrose (DP) and Continuous Spontaneous Localization (CSL) models, predict the emission of spontaneous radiation, which allows experimental tests. Using a high-purity Germanium detector, we could exclude the natural parameterless version of the DP model and put strict bounds on the CSL one. In addition, forbidden atomic transitions could prove a possible violation of the Pauli Exclusion Principle (PEP) in open and closed systems. The VIP-2 experiment is currently in operation, aiming at detecting PEP-violating signals in Copper with electrons; the VIP-3 experiment upgrade is foreseen to become operative in the next few years. We discuss the VIP-Lead experiment on closed systems, and the strong bounds it sets on classes of non-commutative quantum gravity theories, such as the $\theta$–Poincaré theory. Full article

Models of dynamical wave function collapse consistently describe the breakdown of the quantum superposition with the growing mass of the system by introducing non-linear and stochastic modifications to the standard Schrödinger dynamics. Among them, Continuous Spontaneous Localization (CSL) was extensively investigated both theoretically and experimentally. Measurable consequences of the collapse phenomenon depend on different combinations of the phenomenological parameters of the model—the strength $\lambda$ and the correlation length $r_C$—and have led, so far, to the exclusion of regions of the admissible ($\lambda -r_C$) parameters space. We developed a novel approach to disentangle the $\lambda$ and $r_C$ probability density functions, which discloses a more profound statistical insight. Full article

Bronchopulmonary dysplasia (BPD) still represents an important burden of neonatal care. The definition of the disease is currently undergoing several revisions, and, to date, BPD is actually defined by its treatment rather than diagnostic or clinic criteria. BPD is associated with many prenatal and postnatal risk factors, such as maternal smoking, chorioamnionitis, intrauterine growth restriction (IUGR), patent ductus arteriosus (PDA), parenteral nutrition, sepsis, and mechanical ventilation. Various experimental models have shown how these factors cause distorted alveolar and vascular growth, as well as alterations in the composition and differentiation of the mesenchymal cells of a newborn’s lungs, demonstrating a multifactorial pathogenesis of the disease. In addition, inflammation and oxidative stress are the common denominators of the mechanisms that contribute to BPD development. Vascular endothelial growth factor-A (VEGFA) constitutes the most prominent and best studied candidate for vascular development. Animal models have confirmed the important regulatory roles of epithelial-expressed VEGF in lung development and function. This educational review aims to discuss the inflammatory pathways in BPD onset for preterm newborns, focusing on the role of VEGFA and providing a summary of current and emerging evidence. Full article

Radiotherapy (RT) with accelerated beams of charged particles (protons and carbon ions), also known as hadrontherapy, is a treatment modality that is increasingly being adopted thanks to the several benefits that it grants compared to conventional radiotherapy (CRT) treatments performed by means of high-energy photons/electrons. Hence, information about the biomolecular effects in exposed cells caused by such particles is needed to better realize the underlying radiobiological mechanisms and to improve this therapeutic strategy. To this end, Fourier transform infrared microspectroscopy (μ-FT-IR) can be usefully employed, in addition to long-established radiobiological techniques, since it is currently considered a helpful tool for examining radiation-induced cellular changes. In the present study, MCF-10A breast cells were chosen to evaluate the effects of proton exposure using μ-FT-IR. They were exposed to different proton doses and fixed at various times after exposure to evaluate direct effects due to proton exposure and the kinetics of DNA damage repair. Irradiated and control cells were examined in transflection mode using low-e substrates that have been recently demonstrated to offer a fast and direct way to examine proton-exposed cells. The acquired spectra were analyzed using a deconvolution procedure and a ratiometric approach, both of which showed the different contributions of DNA, protein, lipid, and carbohydrate cell components. These changes were particularly significant for cells fixed 48 and 72 h after exposure. Lipid changes were related to variations in membrane fluidity, and evidence of DNA damage was highlighted. The analysis of the Amide III band also indicated changes that could be related to different enzyme contributions in DNA repair. Full article

The present paper proposes a new methodology to characterize the landslide susceptibility of the Reggio Calabria metropolitan area. For this purpose, various factors were used, such as land use, slope, rainfall, elevation, lithology, distance from roads and rivers, and thanks to the use of GIS devices and the AHP method, the landslide risk was defined for the whole territory. The values obtained were classified into four categories: low, moderate, high, and very high. They were then exported into the GIS environment to produce a landslide susceptibility map. The study carried out demonstrates the fragility of the Calabrian territory. From the results obtained, in fact, 66% of the metropolitan territory of Reggio Calabria appears to have a medium–high landslide risk. Full article