Search Results (18)

Over the last 40 years, several studies have provided evidence demonstrating that viral vectors can result in effective gene targeting/insertions in a host’s genome. The traditional approaches of gene knock-down, -out, or -in involve an intensive transgenesis process that is plagued by extensive timescales. Plant viruses have the potential to target specific genes and integrate exogenous DNA molecules at the target locus. Their ability to manipulate a host’s genetic material and become a part of it makes them remarkable agents and helpful for molecular and synthetic biology. In this review, we describe how geminivirus-based vectors can be utilized to overcome traditional transgenesis. We highlight the progress that has been made so far and also discuss the hurdles that hinder the employment of geminivirus-based vectors. Furthermore, we conclude with a comparison of geminivirus-based vectors with other plant-derived vectors. Geminivirus-based vectors stand poised to revolutionize plant genome editing by making nucleic acid manipulation cheaper and easier to deploy, thus lessening the major technical constraints, including homology-directed repair (HDR)-mediated genome editing and time-inefficient tissue culture procedures. The insights given in this review illustrate a broader picture of geminiviral vectors, with an emphasis on engineering plant viruses to ease genome editing practices for crop improvements as well as boost experimental timescales from years to months. Full article

In recent decades, the use of pesticides has become fundamental to agricultural growth. However, the persistent and toxic nature of pesticides has led to significant concerns regarding their ecological and human health consequences. Therefore, for a better understanding of pesticide contamination and its potential risks, here we assessed the levels of five emerging pesticides—acetochlor, imidacloprid, MCPA, atrazine, and allethrin—in soil samples from ponds used for irrigation and in drinking water samples from nearby areas in Lahore, Pakistan. Our findings revealed that 100% of the samples were contaminated, posing substantial ecological and human health risks. Based on the toxic units (TU_sum), all the soil samples showed higher toxic pressure, exceeding acute and chronic toxicity thresholds for earthworms, while 100% of water samples posed chronic toxicity risks to crustaceans and 10% to algae. Pollution index (PI) analysis further classified 100% of the soil samples and 10% of the water samples as highly polluted. These findings show high-pesticide residues in both soil and water and highlight immediate risk assessment and mitigation measures to protect non-target organisms. This preliminary information can be used to adopt risk assessment monitoring programmes and help higher authorities in making policies and guidelines to mitigate the escalating risk for ecology and humans. Full article

This study evaluated the subsequent effect of photoperiods during incubation on post-hatch growth and stress response of commercial broiler chickens. A total of 875 Ross 308 broiler breeder (48 weeks of age) eggs were hatched using different durations (0, 4, 8, 12, 16, 20, and 24 h a day) of dichromatic light [green and red (495 to 750 nm); 2700 K; 250 lux; SUNJIE; China] throughout the whole period of incubation. A total of 50 0-day-old hatched straight run broiler chicks from each photoperiod during incubation were used to evaluate subsequent growth performance (feed intake, body weight, and feed conversion ratio); stress parameters (physical asymmetry, tonic immobility, and vocalization,); welfare traits (feather score and gait score); carcass traits (live weight, dressed weight, carcass yield, liver weight, gizzard weight, heart weight, abdominal fat weight, breast weight, and leg weight); and serum chemistry (globulin, total protein, cholesterol, glucose, and uric acid). There were no influences of photoperiod during incubation on post-hatch growth, stress parameters, welfare, and carcass traits. Heart yield was higher in birds incubated under 20 h light than in those from the 16 h light group. Incubation under different lighting durations also altered blood biochemical profile but did not influence serum globulin and cholesterol levels. It was concluded that under experimental conditions, incubation of broiler eggs under different lighting durations did not impact subsequent post-hatch performance (21–35 d). Full article

Fractional calculus has been a concept used to obtain new variants of some well-known integral inequalities. In this study, our main goal is to establish the new fractional Hermite–Hadamard, and Simpson’s type estimates by employing a differentiable function. Furthermore, a novel class of fractional integral related to prominent fractional operator (Caputo–Fabrizio) for differentiable convex functions of first order is proven. Then, taking this equality into account as an auxiliary result, some new estimation of the Hermite–Hadamard and Simpson’s type inequalities as generalization is presented. Moreover, few inequalities for concave function are obtained as well. It is observed that newly established outcomes are the extension of comparable inequalities existing in the literature. Additionally, we discuss the applications to special means, matrix inequalities, and the q-digamma function. Full article

Eating disorders and excessive attachment to social media are a matter of great concern among youths. This study assessed the prevalence of eating disorders and their association with social media addiction among youths. A descriptive cross-sectional study was conducted on 350 participants aged 14–25 years. Two pre-validated tools were used, i.e., the Eating Attitude Test and the Social Networking Addiction Scale. SPSS was used to analyze the data. Out of the 350 students, 42% had probable eating disorders, and 41.7% had social media addictions. The findings revealed that the chances of having eating disorders were significantly higher among youths who lived in separate places, smoked, and had a family history of eating disorders (p ≤ 0.05). Furthermore, the dieting domain displayed notably higher scores for youths living separately (p ≤ 0.05) and smokers (p ≤ 0.01). Moreover, the scores for bulimia and food preoccupation were significantly higher among participants who were married (p = 0.038), were smokers (p = 0.027), and had a family history of eating disorders (p = 0.001). Higher scores in the oral control domain were reported by females (p ≤ 0.05) and severely obese youths (p ≤ 0.01). Moreover, social media addiction was significantly higher among students aged 18–21 (p ≤ 0.01). Spearman’s correlation revealed that social media addiction has a weak positive relationship with eating disorders (r = 0.133, p ≤ 0.01), particularly bulimia and food preoccupation (r = 0.173, p ≤ 0.001). This reflects the need to address the harmful consequences of social media addiction that might raise the likelihood of developing eating disorders, particularly bulimia nervosa. Full article

Responsible sustainable consumer behavior might serve as a complex pattern of social and environmental issues, consistent with the perspective of sustainability as a construct with both social and environmental pillars. Anchored on signaling theory and attribution theory, this study seeks to unearth a hitherto unexplored mechanism that might translate sustainability marketing into elevated responsible sustainable consumer behavior through the mediating role of brand image. Besides, this study aims to examine the impact of corporate social responsibility, as an intervening variable, to underpin the association between sustainability marketing and brand image. This study analyzes data from five-star and four-star hotel customers using SmartPLS SEM (v 4.0). The findings support our hypotheses that a hotel’s brand image mediates the relationship between sustainability marketing and responsible sustainable consumer behavior. In addition, hotels’ engagement in corporate social responsibility activities moderates the link between sustainability marketing and brand image, such that the association is stronger (weaker) at higher (lower) levels of corporate social responsibility. This study explores the boundary conditions of the sustainability marketing–responsible sustainable consumer behavior link. Hotels can foster responsible sustainable consumer behavior by executing sustainability marketing and engaging in corporate social responsibility activities. Full article

Advances in genome engineering (GE) tools based on sequence-specific programmable nucleases have revolutionized precise genome editing in plants. However, only the traditional approaches are used to deliver these GE reagents, which mostly rely on Agrobacterium-mediated transformation or particle bombardment. These techniques have been successfully used for the past decades for the genetic engineering of plants with some limitations relating to lengthy time-taking protocols and transgenes integration-related regulatory concerns. Nevertheless, in the era of climate change, we require certain faster protocols for developing climate-smart resilient crops through GE to deal with global food security. Therefore, some alternative approaches are needed to robustly deliver the GE reagents. In this case, the plant viral vectors could be an excellent option for the delivery of GE reagents because they are efficient, effective, and precise. Additionally, these are autonomously replicating and considered as natural specialists for transient delivery. In the present review, we have discussed the potential use of these plant viral vectors for the efficient delivery of GE reagents. We have further described the different plant viral vectors, such as DNA and RNA viruses, which have been used as efficient gene targeting systems in model plants, and in other important crops including potato, tomato, wheat, and rice. The achievements gained so far in the use of viral vectors as a carrier for GE reagent delivery are depicted along with the benefits and limitations of each viral vector. Moreover, recent advances have been explored in employing viral vectors for GE and adapting this technology for future research. Full article

In pursuing innovation, eco-friendly deliberate practice will inevitably elevate eco-innovation performance without creative self-efficacy and perceived eco-innovation importance to organizations. This eco-friendly deliberate practice–eco-innovation link is essential because it extends current thinking, treating creative self-efficacy as a causal mechanism and perceived eco-innovation importance as the magnifier of eco-innovation performance. Anchored in social cognitive theory, this study aims to investigate the role of eco-friendly deliberate practice in fueling eco-innovation performance through the mediating role of creative self-efficacy and the moderating role of perceived eco-innovation importance. This study collected 367 responses from the service employees in tourism firms in Pakistan using a time-lagged, i.e., three-wave, research design. The authors analyzed data using a variance-based structural equation model processed in SmartPLS (v 4.0). The findings support the hypothesized relationships, for example, that eco-friendly deliberate practice has a significant positive relationship with eco-innovation performance. In addition, creative self-efficacy significantly mediates the association between eco-friendly deliberate practice and eco-innovation performance. Besides, employees’ perceived eco-innovation importance moderates the eco-friendly deliberate practice–eco-innovation link, such that at high(low) levels of perceived eco-innovation importance, the relationship between eco-friendly deliberate practice and eco-innovation performance is more(less) pronounced. The study examines a hitherto unexplored moderated mediation model to explain under which conditions eco-friendly deliberate practice promotes eco-innovation performance through creative self-efficacy and perceived eco-innovation importance. Full article

Ovarian cancer has the highest mortality rate among gynecologic malignancies, owing to its misdiagnosis or late diagnosis. Identification of its genetic determinants could improve disease outcomes. Conventional Protein Kinase C-γ (PKCγ) dysregulation is reported in several cancers. Similarly, its variant rs1331262028 is also reported to have an association with hepatocellular carcinoma. Therefore, the aim of the present study was to analyze the variant rs1331262028 association with ovarian cancer and to determine its impact on PKCγ’s protein interactions. Association of variation was determined through genotyping PCR (cohort size:100). Protein–protein docking and molecular dynamic simulation were carried out to study the variant impact of PKCγ interactions. The study outcome indicated the positive association of variant rs1331262028 with ovarian cancer and its clinicopathological features. Molecular dynamics simulation depicted the potential influence of variation on PKCγ molecular signaling. Hence, this study provided the foundations for assessing variant rs1331262028 as a potential prognostic marker for ovarian cancer. Through further validation, it can be applied at the clinical level. Full article

The incorporation of communication technology with Smart Grid (SG) is proposed as an optimal solution to fulfill the requirements of the modern power system. A smart grid integrates multiple energy sources or microgrids and is supported by an extensive control and communication network using the Internet of Things (IoT) for a carbon-free, more reliable, and intelligent energy system. Along with many benefits, the system faces novel security challenges, data management, integration, and interoperability challenges. The advanced control and communication network in the smart grid is susceptible to cyber and cyber-physical threats. A lot of research has been done to improve the cyber security of the smart grid. This review aims to provide an overview of the types of cyber security threats present for smart grids with an insight into strategies to overcome the challenges. As the selection of techniques and technologies may vary according to the threats faced, therefore the adoption of researched methods is compared and discussed. As cyber-security is the greatest challenge in smart grid implementation, this review is beneficial during the planning and operation of smart grids for enhanced security. Full article

In recent years, the synthesis of ammonia (NH₃) has been developed by electrocatalytic technology that is a potential way to effectively replace the Haber–Bosch process, which is an industrial synthesis of NH₃. Industrial ammonia has caused a series of problems for the population and environment. In the face of sustainable green synthesis methods, the advantages of electrocatalytic nitrogen reduction for synthesis of NH₃ in aqueous media have attracted a great amount of attention from researchers. This review summarizes the recent progress on the highly efficient electrocatalysts based on 2D non-metallic nanomaterial and provides a brief overview of the synthesis principle of electrocatalysis and the performance measurement indicators of electrocatalysts. Moreover, the current development of N₂ reduction reaction (NRR) electrocatalyst is discussed and prospected. Full article

This paper proposes a solar-powered resonant inverter fed a high-voltage DC power supply. In this converter, switching loss is controlled through zero-voltage switching and zero-current switching. This converter comprises a solar panel, boost converter, full-bridge LLC resonant tank, power transformer, and rectifier circuit. All power switches are operated with an interleaved switching cycle to ensure equal power flow from the tank. This proposed converter is designed to produce a regulated 19.5 KV at output, with an input voltage range of 300–350 V. The proposed converter was simulated in PSpice to verify the results. Full article

This paper introduces and uses a single-phase, high-power LED driver with a battery backup. The buck–boost converter and reverse converter are both combined to achieve optimal performance. In the first part of the integrated circuit, the buck–boost converter is simply used to adjust the power when operating in the non-continuous operating mode. The reverse converter provides free voltage to the LEDs when released as a remote DC–DC converter. The battery backup cycle directly charges the battery at the same power as the LED driver required and provides charging power when there is no electricity. This paper demonstrates the functionality of the entire system and proves that it is an effective solution for new lighting applications. Full article

Technological advancements in solar power systems necessitate highly reliable power inverters with a high efficiency and a small size. An LLC resonant converter-based pseudo Direct Current (DC) link inverters offer these qualities to some extent. The resonant circuits of conventional pseudo DC link inverters lack the ability to attain a zero gain and cannot handle variable frequency control which in turn requires very large filters to produce pure sinusoidal output voltages for grid. The usage of these filters consequences in the enhanced price and size of inverters; moreover, the reliability of inverters is also reduced. We propose a novel topology for a pseudo DC link inverter based on an LLCLC resonant converter. The proposed inverter does not require large filters, because it generates rectified sinusoidal output voltages. An additional parallel LC component is added in series to the resonant circuit, which makes it able to attain a zero gain through an infinite circuit impedance. The 400 W pseudo DC link inverter with a 40 V input and a 400 V output is designed and simulated on OrCAD PSpice software. The results showed that there is a significant improvement in achieving a zero gain. The possible lowest gain achieved is approximately 0.125. The proposed technique attested to be more efficient than those formerly used, subsequently contributing satisfying outcomes. Full article

The present work reports the assessment of the effectiveness of a foliar-spray of salicylic acid (SA) on growth attributes, biochemical characteristics, antioxidant activities and osmolytes accumulation in wheat grown under control (100% field capacity) and water stressed (60% field capacity) conditions. The total available water (TAW), calculated for a rooting depth of 1.65 m was 8.45 inches and readily available water (RAW), considering a depletion factor of 0.55, was 4.65 inches. The water contents corresponding to 100 and 60% field capacity were 5.70 and 1.66 inches, respectively. For this purpose, seeds of two wheat cultivars (Fsd-2008 and S-24) were grown in pots subjected to water stress. Water stress at 60% field capacity markedly reduced the growth attributes, photosynthetic pigments, total soluble proteins (TSP) and total phenolic contents (TPC) compared with control. However, cv. Fsd-2008 was recorded as strongly drought-tolerant and performed better compared to cv. S-24, which was moderately drought tolerant. However, water stress enhanced the contents of malondialdehyde (MDA), hydrogen peroxide (H₂O₂) and membrane electrolyte leakage (EL) and modulated the activities of antioxidant enzymes (superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as accumulation of ascorbic acid (AsA), proline (Pro) and glycine betaine (GB) contents. Foliar-spray with salicylic acid (SA; 0, 3 mM and 6 mM) effectively mitigated the adverse effects of water stress on both cultivars. SA application at 6 mM enhanced the shoot and root length, as well as their fresh and dry weights, and improved photosynthetic pigments. SA foliage application further enhanced the activities of antioxidant enzymes (SOD, POD, and CAT) and nonenzymatic antioxidants such as ascorbic acid and phenolics contents. However, foliar-spray of SA reduced MDA, H₂O₂ and membrane permeability in both cultivars under stress conditions. The results of the present study suggest that foliar-spray of salicylic acid was effective in increasing the tolerance of wheat plants under drought stress in terms of growth attributes, antioxidant defense mechanisms, accumulation of osmolytes, and by reducing membrane lipid peroxidation. Full article