Search Results (92)

Bothus podas (wide-eyed flounder) is a benthic flatfish likely exposed to microplastic (MP) pollution. We investigated MP ingestion and associated physiological effects in wild B. podas collected from Mallorca (Balearic Islands), Spain. Markers of oxidative stress, detoxification, and immunity were quantified in intestinal, hepatic, and splenic tissues. MPs were observed in the gastrointestinal tracts of 87.5% of the 24 specimens analyzed, with an average of 3.8 ± 0.6 items per fish. Fiber-type MPs predominated in both the gastrointestinal tract (69.6%) and sediment samples (97%). Additionally, micro-Fourier transform infrared spectroscopy analysis confirmed that the majority of ingested MPs were composed of polyethylene, polypropylene, and polyester. Fish were categorized into low (<3 items) and high (≥3 items) MP groups based on the median number of plastic items found in the gastrointestinal tract to assess sublethal impacts. In the gut, high-MP fish exhibited significantly elevated activities of detoxification enzymes: ethoxyresorufin-O-deethylase (phase I) and glutathione s-transferase (phase II), along with increased antioxidant enzyme superoxide dismutase and inflammatory myeloperoxidase. Gut catalase and malondialdehyde (MDA) were not significantly different between groups. In liver tissues, no biomarkers differed significantly with MP exposure. In the spleen, lysozyme and alkaline phosphatase activities were significantly higher in high-MP fish, while splenic MDA remained unchanged. These results indicate that gastrointestinal MP exposure triggers local oxidative stress responses and systemic immune activation in B. podas. Overall, ingestion of environmentally relevant MP levels elicited detoxification and inflammatory responses without significant increases in MDA, an indicator of oxidative damage, highlighting the physiological stress imposed by plastic pollution on benthic fish. Full article

Indoors, the infection risk of diseases transmitted through the airborne route is estimated from indoor carbon dioxide (CO₂) levels. However, the approaches to assess this risk do not account for the airborne concentration of pathogens, among other limitations. In this study, we analyzed the relationship between airborne SARS-CoV-2 levels and environmental parameters. Bioaerosols were sampled (n = 40) in hospital corridors of two wards differing in the COVID-19 severity of the admitted patients. SARS-CoV-2 levels were quantified using droplet digital PCR. SARS-CoV-2 was detected in 60% of the total air samples. The ward where the mildly ill patients were admitted had a higher occupancy, transit of people in the corridor, and CO₂ levels, but there were no significant differences in SARS-CoV-2 detection between wards. The mean CO₂ concentration in the positive samples was 569 ± 35.6 ppm. Considering all samples, the CO₂ levels in the corridor were positively correlated with patient door openings but inversely correlated with SARS-CoV-2 levels. In conclusion, airborne SARS-CoV-2 can be detected indoors with optimal ventilation, and its levels do not scale with CO₂ concentration in hospital corridors. Therefore, CO₂ assessment should not be interpreted as a surrogate of airborne viral presence in all indoor spaces. Full article

Plastic pollution poses a massive problem to the environment, particularly seas and oceans. Microplastics (MPs) ingestion by marine species can generate many adverse effects, including causing oxidative stress. This study evaluated the effects of anthropic activity-related MP presence in two coastal fish species—Serranus scriba (more related to rocky bottoms) and Lithognathus mormyrus (more related to sandy bottoms)—in two areas of Mallorca Island (Western Mediterranean) with varying anthropic pressures with similar mixed rocky/sandy bottoms. A total of eight fish samples per species and per area (total n = 32), as well as three water samples (500 mL each) and three sediment samples per area, were collected and analyzed. The results showed that despite plastic presence in both areas, the area with higher tourism affluence was also the most polluted. Fourier transform infrared spectroscopy analysis confirmed that the majority of recovered polymers were polyethylene and polypropylene. The pattern of MPs presence was reflected in the biomarker analysis, which showed higher values of antioxidants, namely catalase (CAT) and superoxide dismutase (SOD); detoxification, namely glutathione s-transferase (GST); and inflammation, namely myeloperoxidase (MPO)—enzymes in the gastrointestinal tract of fish from the more polluted area. However, no statistical differences were found for malondialdehyde (MDA) as a marker of lipid peroxidation. As for differences between species, S. scriba presented a higher presence of MPs and measured biomarkers than in L. Mormyrus, suggesting higher exposure. In conclusion, these results showed that increased anthropic activity is associated with a higher presence of MPs which, in turn, induces an adaptative response in exposed fish. Moreover, species living in the same area could be differentially affected by MPs, which is probably associated with different behavioural and feeding habits. Full article

Microplastic (MP) pollution is a growing environmental concern, particularly in marine ecosystems. This study investigated the presence and effects of MPs in the common dentex (Dentex dentex), a commercially and ecologically important species in the Mediterranean Sea. Fish (n = 22) were collected from Ibiza’s coastal waters (western Mediterranean, Spain), and their gastrointestinal tracts were analysed to determine MP ingestion rates and potential physiological impacts. MPs were detected in 90.9% of the specimens, with an average of 6.6 ± 1.2 MPs per individual. Fibres accounted for 78.9% and fragments for 21.1%. Stress and detoxification biomarkers were assessed by categorizing fish into two groups based on MP load: low (<6 MPs) and high (≥6 MPs). Fish with higher MP loads showed significantly increased detoxification and antioxidant enzyme activities in the digestive tract, while malondialdehyde levels remained similar between groups. No significant differences were observed in liver biomarkers. These findings indicate that MP ingestion triggers physiological responses in D. dentex, activating antioxidant and detoxification defences in the digestive tract. However, the lack of response in the liver suggests that the MP load is not sufficient to induce systemic changes. Further research is needed to assess long-term consequences on fish health and ecosystem sustainability. Full article

(1) Background: Respiratory viral infections, including those caused by SARS-CoV-2, respiratory syncytial virus (RSV), influenza viruses, rhinovirus, and adenovirus, are major causes of acute respiratory infections (ARIs) in children. Symptom-based predictive models are valuable tools for expediting diagnoses, particularly in primary care settings. This study assessed the effectiveness of machine learning-based models in estimating infection probabilities for these common pediatric respiratory viruses, using symptom data. (2) Methods: Data were collected from 868 children with ARI symptoms evaluated across 14 primary care centers, members of COPEDICAT (Coronavirus Pediatria Catalunya), from October 2021 to October 2023. Random forest and boosting models with 10-fold cross-validation were used, applying SMOTE-NC to address class imbalance. Model performance was evaluated via area under the curve (AUC), sensitivity, specificity, and Shapley additive explanations (SHAP) values for feature importance. (3) Results: The model performed better for RSV (AUC: 0.81, sensitivity: 0.64, specificity: 0.77) and influenza viruses (AUC: 0.71, sensitivity: 0.70, specificity: 0.59) and effectively ruled out SARS-CoV-2 based on symptom absence, such as crackles and wheezing. Predictive performance was lower for non-enveloped viruses like rhinovirus and adenovirus, due to their nonspecific symptom profiles. SHAP analysis identified key symptoms patterns for each virus. (4) Conclusions: The study demonstrated that symptom-based predictive models effectively identify pediatric respiratory infections, with notable accuracy for those caused by RSV, SARS-CoV-2, and influenza viruses. Full article

Organic waste treatment, including its many strategies and objectives, is one of the most rapidly changing sectors of environmental technology. It is closely related to sustainability and other critical issues, such as global warming. The first years of this century were the time of a transition from traditional technologies for the “disposal of” waste, such as incineration (with poor energy recovery) or landfill (more or less controlled), to biotechnologies that are more profitable, such as composting and anaerobic digestion. However, recent developments are focused on advanced technologies in the framework of a circular bioeconomy, maximizing the production of biomaterials and renewable energy using raw organic waste or digested materials. This perspective paper delves into the second transition in the field of technologies for treating and valorizing organic waste, highlighting emerging technologies such as anaerobic digestion enhanced with nanomaterials or biochar to substitute fossil natural gas, solid-state fermentation to obtain bioproducts that have a “chemical twin” with a high environmental impact, and pyrolysis as a predominant thermal treatment due to the production of biochar, probably the most promising biomaterial in today’s research. All these technologies exploit the potential of organic waste for bioenergy production and material utilization, in line with circular principles. Full article

Background: In 1994, the first Spanish study on patients with self-inflicted burns (SIB) was published, showing a prototypical profile of a patient with SIB: adult male, unmarried and, in 75% of the cases, with a psychiatric background. In addition, SIB accounted for 1.98% of the total admissions in a Burns Unit between 1983 and 1991, a lower percentage than other European studies. The present study aims to replicate this work, updating this profile and comparing it with the current profile. Methods: We compared the clinical and socio-demographic characteristics of 67 patients admitted during 1983–1991 (Study I) with those of 36 patients admitted during 2010–2015 (Study II). Results: It was observed that the percentage of patients with SIB admitted to the Burns Unit was lower in Study II than in Study I (1.45% vs. 1.98%). Significant age differences were identified (t₍₁₀₁₎ = −2.074, p = 0.041, 95% CI [−11.739, −0.261]). Similarly, there were statistically significant differences in several clinical characteristics, such as psychiatric history (X² = 11.591, p = 0.001), the occurrence of previous autolytic attempts (X² = 7.714, p = 0.007), the place where the incident occurred (X² = 11.647, p = 0.020), the etiology of the burn (X² = 13.142, p = 0.004), and triggers (X² = 6.420, p = 0.036). Conclusions: Several differences have arisen between the two studies, mainly related to the specific characteristics of SIB (e.g., etiology, triggering cause, and place of the incident), possibly attributable to the social changes that have occurred in the last 20 years. These results will add to our knowledge and will stress various precipitating factors that may lead to SIB, with the final goal of designing preventive strategies. Full article

Agro-industrial activities generate significant amounts of organic waste and a variety of effluents thus posing environmental challenges. Viticulture in Argentina, which covered 204,847 ha in 2023, faces water scarcity as a limiting factor conditioning its production. This industry produces large volumes of grape marc, sediments, and stalks, which can be valorised into products like alcohol, tartaric acid, and compost. However, these valorisation processes generate effluents with high organic load and salinity, further stressing water resources. This study explores the potential of utilising these effluents to cultivate plant biomass in arid regions (sorghum or perennial pasture), which could serve as bioenergy, animal feed, or composting co-substrates, contributing to circular bioeconomy principles. The combined use of effluent as a water resource and the sowing of sorghum and pasture increased soil organic matter content and led to a slight reduction in pH (depth: 0.30–0.60 m) compared to the control treatment. The sorghum plots showed better establishment and higher dry biomass yield (32.6 Tn/ha) compared to the pasture plots (6.5 Tn/ha). Sorghum demonstrated better tolerance to saline soils and high salinity effluents, aligning with previous studies. Although pasture had a lower biomass yield, it was more efficient in nutrient uptake, concentrating more NPK, ash, and soluble salts. Sorghum’s higher yield compensated for its lower nutrient concentration. For biomass production, sorghum is preferable, but if nutrient capture from effluents is prioritised, summer polyphytic pastures are more suitable. These results suggest that the final selection between plant biomass alternatives highly depends on whether the goal is biomass generation or nutrient capture. Full article

In urban logistics, effective maintenance is crucial for maintaining the reliability and efficiency of energy supply systems, impacting both asset performance and operational stability. This paper addresses the scheduling and routing plans for maintenance of power generation assets over a multi-period horizon. We model this problem as a multi-period team orienteering problem. To address this multi-period challenge, we propose a dual approach: a novel reinforcement learning (RL) framework and a biased-randomized heuristic algorithm. The RL-based method dynamically learns from real-time operational data and evolving asset conditions, adapting to changes in asset health and failure probabilities to optimize decision making. In addition, we develop and apply a biased-randomized heuristic algorithm designed to provide effective solutions within practical computational limits. Our approach is validated through a series of computational experiments comparing the RL model and the heuristic algorithm. The results demonstrate that, when properly trained, the RL-based model is able to offer equivalent or even superior performance compared to the heuristic algorithm. Full article

The packed-bed bioreactor is among the most promising reactor configurations for solid-state fermentation. However, the bed thickness poses several limitations involving mass and energy transfer, heat generation, and the homogeneity of the material, hampering its development at the industrial scale. Fungi are among the most promising microorganisms used in this configuration; however, only polypropylene bags and trays are used at the industrial scale. In this work, Beauveria bassiana is used to demonstrate the potential of solid-state fermentation for conidia production. A scale-up from 0.5 L to 22 L is presented, starting with substrate selection, optimization via design of experiments and 22 L batches. The optimized parameters were 70% moisture, 6.5 × 10⁶ conidia mL⁻¹ inoculum concentration, 20 mL min⁻¹ airflow, 25 °C; temperature, and 40 C/N ratio. After optimization, beer draff was chosen as the preferred substrate for scale-up. Air-filled porosity was found to be the key parameter in fungal solid-state fermentation scale-up, establishing values of around 80% as necessary for fungal conidia production when working in a 22 L packed-bed bioreactor. When compared with the tray bioreactor, the packed bed obtained higher conidia production due to its better use of the total reactor volume. Our study harnesses the potential of the packed-bed bioreactor and serves as a base for further scale-up to industrial scale. Full article

The increasing emission of carbon dioxide into the atmosphere has urged the scientific community to investigate alternatives to alleviate such emissions, being that they are the principal contributor to the greenhouse gas effect. One major alternative is carbon capture and utilization (CCU) toward the production of value-added chemicals using diverse technologies. This work aims at the study of the catalytic potential of different cobalt-derived nanoparticles for methanol synthesis from carbon dioxide hydrogenation. Thanks to its abundance and cost efficacy, cobalt can serve as an economical catalyst compared to noble metal-based catalysts. In this work, we present a systematic comparison among different cobalt and cobalt oxide nanocomposites in terms of their efficiency as catalysts for carbon dioxide hydrogenation to methanol as well as how different supports, zeolites, MnO₂, and CeO₂, can enhance their catalytic capacity. The oxygen vacancies in the cerium oxide act as carbon dioxide adsorption and activation sites, which facilitates a higher methanol production yield. Full article

Alcohol content, proanthocyanins and anthocyanins influence wine quality. The composition of wine depends on the type of cultivar, location, environmental conditions, and management practices. Phenolic compounds have attracted considerable research interest due to their antioxidant properties and potential beneficial effects on human health. However, the low bioavailability of anthocyanins creates a major bottleneck in their ability to exert beneficial effects. Despite extensive research on the effects of wine on human health, no clear evidence has been obtained on the benefits of wine quality or geographic area of production on health conditions, such as metabolic syndrome. Five climatically and geologically distinct wines were evaluated. Based on recent studies, meta-analyses, and pooled analyses of wine composition, along with the predicted low bioavailability of polyphenol compounds, we estimated the efficacy of five geographically distinct wines according to gastrointestinal absorption and the effects of alcohol intake on both men and women, with a view to ascertaining whether geographical origin influences the antioxidant serum composition of wine. Data on the estimated consumption of wine suggest that the polyphenol contents are similar regardless of choice of wine/area, while different alcohol compositions affect the level of alcohol and calorie intake. Thus, moderate wine drinkers should be advised to control the habit, but without exceeding the dose considered a healthy threshold (up to 30–40 g of alcohol/day in men and 10–20 g of alcohol/day in women), given no medical contraindications are present. These results will add value to the framework of the last reform of the Common Agricultural Policy (CAP) adopted in December 2021, where the European Parliament and the Council introduced new labeling rules for the wine sector and aromatized wine products. Full article

Climate variability in Mediterranean viticultural areas, primarily attributed to climate change, will significantly impact water requirements, consequently leading to changes in irrigation management. The primary aim of this study was to assess the response of the Tempranillo and Cabernet Sauvignon grape varieties when subjected to deficit drip irrigation (RDI), in comparison to non-irrigation (NI), during various climatic years. The defined irrigation strategies involved water application equivalent to 35% and 80% of the (ET0 (reference crop evapotranspiration) × Kc (crop coefficient)). The ecophysiology of both grapevines was evaluated through the measurement of stomatal conductance (gs), sap flow, transpiration, leaf water potential (LWP), and CO₂ assimilation (A). Additionally, essential parameters including the crop coefficient (Kc), transpiration, and intrinsic water use efficiency were calculated. The information gathered from the pressure–volume curves of Cabernet Sauvignon and Tempranillo encompassed the osmotic potential at full turgor (γπ100), osmotic potential at turgor loss or 0 turgor (γπ0), water content at turgor loss (CHR0), modulus of elasticity (ε), and water potential at turgor loss 0 (γ_H0). The results enable a precise estimation of the water requirements for irrigation, contributing to a deeper understanding of the physiological responses of both varieties. This comprehension aids in assessing the sustainability of these vineyards amidst unexpected changes in the global mean surface temperature. Full article

Global change produces substantial modification to the distribution and rhythm of infection of diseases in fish, favouring the introduction of new pathogens. Recently, the presence of black spot disease, associated with a digenean fluke of the genus Scaphanocephalus, has been observed in specimens of Xyrichthys novacula on the island of Ibiza (Balearic Islands). The aim of the present study was to evaluate the antioxidant and immune response in both the skin mucus and spleen of X. novacula depending on the degree of infection by Scaphanocephalus sp. The specimens were captured in a control area, without the presence of the parasite, and in an affected area, classifying the fish as low infection (1–15 spots) and high infection (>15 spots). As the degree of infection increased, a decrease in the body condition index was observed. The activity of the antioxidant enzymes in the mucus—catalase, superoxide dismutase and glutathione peroxidase—increased progressively with the degree of infection. This activation of antioxidant defences was not enough to prevent an increase in malondialdehyde levels, an indicator of oxidative damage, in the group with the highest infection. Similarly, an increase in immunological parameters—lysozyme, alkaline phosphatase, myeloperoxidase and immunoglobulins—was observed in mucus as infection increased. Regarding the spleen, only an increase in lysozyme activity and alkaline phosphatase in fish with a greater severity of infection was observed. In conclusion, as the severity of Scaphanocephalus sp. infection increased, it induced an immune and oxidative stress response in skin mucus, leading to a decrease in overall body condition. The potential health effects that the ectoparasite may have on X. novacula populations will require follow-up studies. Full article