Cytoprotective Effect of Epigallocatechin Gallate (EGCG)-5′-O-α-Glucopyranoside, a Novel EGCG Derivative
Abstract
:1. Introduction
2. Results
2.1. Antioxidant Effect of EGCG-5′Glu in Cell and Cell-free Systems
2.2. Cell Protective Effect of EGCG-5′Glu from SNP-Induced Radicals
2.3. Cytoprotective Effect of EGCG-5′Glu Against UVB-Induced Damage
2.4. Cell Proliferative Effect of EGCG-5′Glu
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Culture
4.3. Cell Viability and Cell Proliferation Assay
4.4. DPPH assay
4.5. ABTS Assay
4.6. ROS Generation
4.7. NO Production and Griess Assay
4.8. Immunoblot Assay
4.9. UVB Irradiation
4.10. Reporter Gene Assay
4.11. Statistical Analysis
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Han, S.Y.; Kim, E.; Hwang, K.; Ratan, Z.A.; Hwang, H.; Kim, E.-M.; Kim, D.; Park, J.; Cho, J.Y. Cytoprotective Effect of Epigallocatechin Gallate (EGCG)-5′-O-α-Glucopyranoside, a Novel EGCG Derivative. Int. J. Mol. Sci. 2018, 19, 1466. https://doi.org/10.3390/ijms19051466
Han SY, Kim E, Hwang K, Ratan ZA, Hwang H, Kim E-M, Kim D, Park J, Cho JY. Cytoprotective Effect of Epigallocatechin Gallate (EGCG)-5′-O-α-Glucopyranoside, a Novel EGCG Derivative. International Journal of Molecular Sciences. 2018; 19(5):1466. https://doi.org/10.3390/ijms19051466
Chicago/Turabian StyleHan, Sang Yun, Eunji Kim, Kyeonghwan Hwang, Zubair Ahmed Ratan, Hyunsik Hwang, Eun-Mi Kim, Doman Kim, Junseong Park, and Jae Youl Cho. 2018. "Cytoprotective Effect of Epigallocatechin Gallate (EGCG)-5′-O-α-Glucopyranoside, a Novel EGCG Derivative" International Journal of Molecular Sciences 19, no. 5: 1466. https://doi.org/10.3390/ijms19051466