Function of the ERFL1a Transcription Factor in Wheat Responses to Water Deficiency
Abstract
:1. Introduction
2. Results and Discussion
2.1. Identification of TaERFL1a
2.2. Expression Profiles of TaERFL1a during the Responses of Wheat to Abiotic Stresses and Abscisic Acid
2.3. Function of TaERFL1a in the Response of Wheat to Abiotic Stresses
2.4. Subcellular Localization of TaERFL1a
2.5. Interacting Proteins of TaERFL1a
3. Materials and Methods
3.1. Identification and Sequence Analysis of TaERFL1a
3.2. Plant Materials and Abiotic Stresses
3.3. Transcript Level of TaERFL1a
3.4. Transient Silencing of TaERFL1a
3.5. Subcellular Localization of TaERFL1a
3.6. Transcription Activation of, and Proteins Interacting with TaERFL1a
3.7. Transcript Levels of the Genes Encoding Six Identified Proteins
3.8. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Treatments | Fresh Weights (g/Plant) | Dry Weights (g/Plant) | Absolute Water Contents (%) | MDA Concentrations (nmol·L−1 FW) |
---|---|---|---|---|
BSMV-GFP | 0.49 ± 0.024 a | 0.08 ± 0.007 a | 84.42 ± 0.650 a | 18.98 ± 1.144 a |
BSMV-TaERFL1a | 0.22 ± 0.006 b | 0.06 ± 0.020 b | 74.56 ± 0.912 b | 31.80 ± 0.639 b |
No. | Homology Accession No. | Homologous Protein | Plant Species | Clone Numbers | Homology |
---|---|---|---|---|---|
1 | JQ269674 | Cu/Zn Superoxide dismutase | Triticum aestivum | 1 | 97.8% |
2 | KJ907387.1 | SGT1 | Triticum aestivum | 1 | 99.3% |
3 | GU564291 | Defender against cell death 2 | Triticum aestivum | 1 | 100.0% |
4 | XM_020309713 | Pyrrolidone-carboxylate peptidase | Aegilops tauschii | 1 | 85.6% |
5, 6, 7 | AM087943 | Avenin-like a precursor | Aegilops tauschii | 3 | 93.2% |
8, 9, 10 | XM_020326125 | MOB kinase activator-like | Aegilops tauschii | 3 | 95.3% |
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Gao, T.; Li, G.-Z.; Wang, C.-R.; Dong, J.; Yuan, S.-S.; Wang, Y.-H.; Kang, G.-Z. Function of the ERFL1a Transcription Factor in Wheat Responses to Water Deficiency. Int. J. Mol. Sci. 2018, 19, 1465. https://doi.org/10.3390/ijms19051465
Gao T, Li G-Z, Wang C-R, Dong J, Yuan S-S, Wang Y-H, Kang G-Z. Function of the ERFL1a Transcription Factor in Wheat Responses to Water Deficiency. International Journal of Molecular Sciences. 2018; 19(5):1465. https://doi.org/10.3390/ijms19051465
Chicago/Turabian StyleGao, Tian, Ge-Zi Li, Chuan-Ren Wang, Jie Dong, Sha-Sha Yuan, Yong-Hua Wang, and Guo-Zhang Kang. 2018. "Function of the ERFL1a Transcription Factor in Wheat Responses to Water Deficiency" International Journal of Molecular Sciences 19, no. 5: 1465. https://doi.org/10.3390/ijms19051465