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The Innovations in Anaerobic Digestion Technology

A special issue of Water (ISSN 2073-4441). This special issue belongs to the section "Wastewater Treatment and Reuse".

Deadline for manuscript submissions: 31 January 2026 | Viewed by 593

Special Issue Editors


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Guest Editor
College of Environmental Science & Engineering, Beijing Forestry University, Beijing, China
Interests: wastewater treatment; resource recovery; nutrient removal; iron biochemistry; net zero upgrading

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Guest Editor
College of Environment and Resources, Xiangtan University, Xiangtan, China
Interests: water pollution; groundwater pollution; water treatment

Special Issue Information

Dear Colleagues,

Anaerobic digestion (AD) plays a crucial role in wastewater management, with its importance being widely proved by the ability to manage organic waste, reduce greenhouse gas emissions, and generate renewable energy, aligning with global sustainability goals.

In the last decade, scientists have been working on innovations in AD to improve efficiency and scalability. The achieved advancements have included enhanced pre-treatment methods, advanced digester designs, and the integration of AD with other processes, such as resource recovery and carbon capture, which have significantly increased biogas yields and operational stability. Additionally, developments in monitoring and control systems, including IoT and machine learning, have optimized the digestion process and enabled real-time data analysis for better management. Emerging trends also include the co-digestion of various feedstocks, such as food waste, manure, and agricultural residues, which further boosts biogas production while providing a sustainable solution for waste reduction and resource recovery.

With this Special Issue of Water, we provide a platform for the publication of original articles and reviews regarding the innovations in anaerobic digestion technology. The scope of this Special Issue includes, but is not limited to, the following: anaerobic treatment of municipal, agricultural, and industrial wastewater; pre- and post-treatment strategies for AD; novel and integrated anaerobic processes; biogas upgrading; recovery of value-added products from wastewaters; economic and environmental sustainability analysis in AD; and the circular bioeconomy concept in AD. This Special Issue will offer valuable insights into cutting-edge issues in AD, making this collection a pivotal resource for those seeking the latest developments in anaerobic technologies.

Prof. Dr. Xiang Cheng
Prof. Dr. Yin Xu
Guest Editors

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Keywords

  • process enhancement
  • hybrid configuration
  • pre- and post-treatment
  • co-digestion
  • biogas upgrading
  • resource recovery
  • circular economy

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Published Papers (1 paper)

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Research

16 pages, 3216 KB  
Article
Riboflavin-Functionalized Conductive Material Enhances a Pilot-Scaled Anaerobic Digester Fed with Cattle Manure Wastewater: Synergies on Methanogenesis and Methanosarcina barkeri Enrichment
by Guangdong Sun, Yiwei Zeng, Qingtao Deng, Jianyong Ma, He Dong, Haowen Zhang, Hao He, Haiyu Xu, Hongbin Wu and Yan Dang
Water 2025, 17(20), 2967; https://doi.org/10.3390/w17202967 - 15 Oct 2025
Viewed by 248
Abstract
Anaerobic digestion (AD) technology is universally acknowledged as the most economically viable and efficient approach for energy recovery from livestock manure. To validate the efficacy of riboflavin-functionalized carbon-based conductive materials (CCM-RF) in enhancing methane production at pilot scale, three pilot-scale upflow anaerobic sludge [...] Read more.
Anaerobic digestion (AD) technology is universally acknowledged as the most economically viable and efficient approach for energy recovery from livestock manure. To validate the efficacy of riboflavin-functionalized carbon-based conductive materials (CCM-RF) in enhancing methane production at pilot scale, three pilot-scale upflow anaerobic sludge blanket (UASB) reactors were constructed and separately supplemented with carbon cloth (CC), granular activated carbon (GAC), and a combination of CC and GAC. During reactor initialization, riboflavin and a concentrated inoculum of Methanosarcina barkeri (M. barkeri) were introduced to investigate the mechanistic role of CCM-RF in promoting direct interspecies electron transfer (DIET) and optimizing treatment efficiency during anaerobic digestion of cattle manure wastewater. The results showed that all reactors improved AD performance and maintained stable operation at the OLR of 15.66 ± 1.95 kg COD/(m3·d), with a maximum OLR of 20 kg COD/(m3·d) and the HRT as short as 5 days. Among the configurations, the CC reactor outperformed the others, achieving a methane volumetric yield of 6.42 m3/(m3·d), which represents an eight-fold increase compared to conventional AD systems. Microbial community analysis revealed that, although M. barkeri was initially inoculated in large quantities, Methanothrix—a methanogen with DIET capability—eventually became the dominant species. The enrichment of Methanothrix and the simultaneous enhancement in sludge conductivity collectively verified the mechanistic role of CCM-RF in promoting CO2-reductive methanogenesis through strengthened DIET pathways. Notably, M. barkeri showed progressive proliferation under conditions of high organic loading rates (OLR) and short hydraulic retention time (HRT). This phenomenon provides a critical theoretical basis for the development of future strategies aimed at the targeted enrichment of Methanosarcina-dominant microbial consortia. Full article
(This article belongs to the Special Issue The Innovations in Anaerobic Digestion Technology)
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