Analytical Tools to Study Biomarkers

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (30 November 2017) | Viewed by 4617

Special Issue Editor


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Guest Editor
Lyon Neuroscience Research Center, Inserm U1028, CNRS UMR5292, University of Lyon, France
Interests: brain; neurotransmitters; microsampling; capillary electrophoresis; liquid chromatography; in vivo; ex vivo

Special Issue Information

Dear Colleagues,

Analytical tools for the detection and quantification of biomarkers are, nowadays, very challenging since these compounds, present in accessible body fluids or tissues, can be used to reveal and diagnose alterations or dysfunctions in many physio-pathological states. In this context, sampling methods, pre-analytical treatments, separation and detection approaches are crucial to relevantly study biomarkers in either human diseases or experimental models.

We would like to invite colleagues to contribute with original research articles and reviews to the present Special Issue that aims to show the broad spectrum of analytical possibilities in biomarker monitoring. We are greatly interested in papers describing new methods with a focus on robustness in case of highly original approaches. The development of methods dealing with new potential biomarkers in preclinical or diagnosis studies in any field are also welcome.

Dr. Sandrine Parrot
Guest Editor

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Keywords

  • biomarkers
  • separation
  • sampling
  • pretreatment
  • separation
  • detection
  • diagnosis
  • body fluids
  • tissues

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Published Papers (1 paper)

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Article
Advances and Pitfalls in the Capillary Electrophoresis Analysis of Aggregates of Beta Amyloid Peptides
by Luc Denoroy and Sandrine Parrot
Separations 2018, 5(1), 2; https://doi.org/10.3390/separations5010002 - 22 Dec 2017
Cited by 1 | Viewed by 4314
Abstract
Alzheimer’s disease is characterized by the accumulation of brain amyloid plaques composed of aggregates of amyloid β (Aβ) peptides. The present paper describes a novel and easy-to-run capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method for the specific analysis of fibrillar forms of [...] Read more.
Alzheimer’s disease is characterized by the accumulation of brain amyloid plaques composed of aggregates of amyloid β (Aβ) peptides. The present paper describes a novel and easy-to-run capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method for the specific analysis of fibrillar forms of Aβ aggregates obtained after in vitro incubation of Aβ 1-40 monomer. For that purpose, an affinity CE-LIF approach in which the ligand thioflavine T was added to the running buffer has been used, leading to the separation and detection of various fibrillar aggregates which migrated as spikes. The procedure has been optimized to get spikes only corresponding to Aβ aggregates, through the careful elimination of interfering factors and the electrophoretic validation of the link between spikes and particulate material. This method exhibited semi-quantification capabilities, led to the separation of Aβ fibrillar aggregates of different sizes and showed that highly concentrated solutions of Aβ peptides led to the formation of aggregates of larger size than lower-concentrated solution did. Advances brought by this method as well as future development needed to overcome its present limitations are discussed. Full article
(This article belongs to the Special Issue Analytical Tools to Study Biomarkers)
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