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Advances and Pitfalls in the Capillary Electrophoresis Analysis of Aggregates of Beta Amyloid Peptides

1
BioRaNTeam, Lyon Neuroscience Research Center, INSERM U1028; CNRS UMR5292; Université de Lyon, F-69000 Lyon, France
2
NeuroDialytics Unit, Lyon Neuroscience Research Center, INSERM U1028; CNRS UMR5292; Université de Lyon, F-69000 Lyon, France
*
Author to whom correspondence should be addressed.
Separations 2018, 5(1), 2; https://doi.org/10.3390/separations5010002
Received: 20 October 2017 / Revised: 1 December 2017 / Accepted: 14 December 2017 / Published: 22 December 2017
(This article belongs to the Special Issue Analytical Tools to Study Biomarkers)
Alzheimer’s disease is characterized by the accumulation of brain amyloid plaques composed of aggregates of amyloid β (Aβ) peptides. The present paper describes a novel and easy-to-run capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) method for the specific analysis of fibrillar forms of Aβ aggregates obtained after in vitro incubation of Aβ 1-40 monomer. For that purpose, an affinity CE-LIF approach in which the ligand thioflavine T was added to the running buffer has been used, leading to the separation and detection of various fibrillar aggregates which migrated as spikes. The procedure has been optimized to get spikes only corresponding to Aβ aggregates, through the careful elimination of interfering factors and the electrophoretic validation of the link between spikes and particulate material. This method exhibited semi-quantification capabilities, led to the separation of Aβ fibrillar aggregates of different sizes and showed that highly concentrated solutions of Aβ peptides led to the formation of aggregates of larger size than lower-concentrated solution did. Advances brought by this method as well as future development needed to overcome its present limitations are discussed. View Full-Text
Keywords: amyloid β peptides; peptides aggregates; capillary electrophoresis; laser-induced fluorescence detection; thioflavine T; Alzheimer’s disease amyloid β peptides; peptides aggregates; capillary electrophoresis; laser-induced fluorescence detection; thioflavine T; Alzheimer’s disease
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Denoroy, L.; Parrot, S. Advances and Pitfalls in the Capillary Electrophoresis Analysis of Aggregates of Beta Amyloid Peptides. Separations 2018, 5, 2.

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