Laboratory Diagnostics in Epidemiological Diseases Caused by Animal Pathogens

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Epidemiology of Infectious Diseases".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 1544

Special Issue Editors


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Guest Editor
National Veterinary Research Institute, Partyzantow Ave. 57, 24-100 Pulawy, PL, Poland
Interests: veterinary microbiology; foodborne pathogens; Clostridial diseases; Clostridium botulinum; molecular biology
Special Issues, Collections and Topics in MDPI journals

E-Mail Website
Guest Editor
National Veterinary Research Institute, Partyzantow Ave. 57, 24-100 Pulawy, PL, Poland.
Interests: veterinary microbiology; molecular biology; infectious disease; cattle and horse viruses; veterinary virology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Animal pathogens are pathogenic agents in wild and domestic animal species that can be characterized by various modes of transmission. They comprise a very diverse group of microorganisms, including viruses, bacteria, and pathogenic species of fungi and yeasts, as well as parasites. Specific species of these microorganisms, through either their entry into the host or their ability to produce a range of toxins, can cause not only the occurrence of serious disease entities that pose a deadly threat to animals but also a threat to the health or life of consumers of products of animal origin.

Laboratory diagnostics focusing on the identification of pathogens play an important role in the effective treatment of diseases in animals and in managing strategies for handling samples in many cases of epidemiological outbreaks. Currently, techniques commonly used in diagnostics include traditional and biochemical methods, as well as molecular biology methods, such as polymerase chain reaction (PCR), and more advanced techniques such as high-throughput sequencing (NGS), which is used in epidemiological investigations to identify the sources and pathways of animal diseases. However, there is an ongoing need to develop new methods to allow the fastest and most accurate diagnosis of disease entities occurring in animals.

The purpose of this Special Issue is to gather articles presenting the latest knowledge on methods used in the laboratory diagnosis of diseases caused by animal pathogens. We encourage the submission of original research and review papers in this area. Papers on the following topics are especially welcome:

-laboratory diagnostics in diseases caused by infectious animal pathogens;

-laboratory diagnostics in non-infectious diseases caused by animal pathogens;

-the laboratory management of epidemiological outbreaks;

-laboratory diagnostics in zoonoses;

-laboratory diagnostics in animal diseases caused by microbial toxins;

-new trends in laboratory procedures used for the detection of pathogens.

Dr. Tomasz Grenda
Dr. Karol Stasiak
Guest Editors

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Keywords

  • animal pathogens
  • laboratory diagnostics
  • epidemiology
  • infections
  • toxins

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Published Papers (2 papers)

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Review

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12 pages, 622 KiB  
Review
Is African Swine Fever Driven by Flying Hematophagous Insects?
by Marek Walczak, Maciej Frant, Krzesimir Szymankiewicz, Małgorzata Juszkiewicz, Katarzyna Podgórska and Marcin Smreczak
Pathogens 2025, 14(6), 563; https://doi.org/10.3390/pathogens14060563 - 5 Jun 2025
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Abstract
African swine fever (ASF) has become one of the most economically important diseases affecting swine and has a significant negative impact on the global pork production sector. In Europe, the main reservoir of the disease is the wild boar population, which poses a [...] Read more.
African swine fever (ASF) has become one of the most economically important diseases affecting swine and has a significant negative impact on the global pork production sector. In Europe, the main reservoir of the disease is the wild boar population, which poses a risk of transmitting the disease to pig farms. To date, no safe and effective vaccine is available on the market. Therefore, biosecurity measures and early recognition of the disease play a key role in preventing and combating ASF. In recent years, numerous insights into the nature of the virus have emerged; however, several knowledge gaps still need to be addressed. One of these gaps is an accurate understanding of all possible pathways through which the virus can reach a pig farm. Interrupting these pathways would significantly reduce the risk of disease outbreaks. Despite a general understanding of disease transmission, ASF can still affect farms with well-established high biosecurity measures. This article highlights the potential for mechanical transmission of ASF by flying hematophagous insects, considering several factors, including current knowledge of the putative role of insects in ASF transmission, insects’ abilities to transmit the virus, ASFV properties, the uncertainties regarding the effectiveness of indirect transmission, and the seasonality of disease outbreaks on domestic pig farms. Full article
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9 pages, 1118 KiB  
Case Report
Laboratory Diagnosis of Animal Tuberculosis in Tracing Interspecies Transmission of Mycobacterium bovis
by Ewelina Szacawa, Nina Kozieł, Sylwia Brzezińska, Ewa Augustynowicz-Kopeć, Marcin Weiner, Krzysztof Szulowski and Monika Krajewska-Wędzina
Pathogens 2025, 14(5), 459; https://doi.org/10.3390/pathogens14050459 - 7 May 2025
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Abstract
Mycobacterium is one of the most dangerous pathogens of both animals and humans. Bovine tuberculosis (BTB) is a disease caused by mycobacteria belonging to the Mycobacterium tuberculosis complex (MTBC), which spreads mainly among domestic cattle but also to mammals other than cattle. The [...] Read more.
Mycobacterium is one of the most dangerous pathogens of both animals and humans. Bovine tuberculosis (BTB) is a disease caused by mycobacteria belonging to the Mycobacterium tuberculosis complex (MTBC), which spreads mainly among domestic cattle but also to mammals other than cattle. The transmission of MTBC between different species requires research and epidemiological investigations to control its spread. When multiple species are a reservoir of infection, it poses a significant public health and veterinary concern. In this study, the diagnosis of alpaca, cattle, horses, dogs, a sheep and a cat from one farm suspected of bovine tuberculosis was performed. The animals (except for one horse, the dogs and the cat) were euthanised after the intradermal tuberculin tests. Mycobacterial isolation from animal tissue samples was performed. The obtained Mycobacterium strains were genotyped using spoligotyping and mycobacterial interspersed repetitive unit–variable number tandem repeat (MIRU-VNTR) methods. The isolates from a horse, two cows, a sheep and an alpaca were classified as Mycobacterium (M.) bovis. The single M. bovis spoligotype SB0666 pattern was isolated, and the MIRU-VNTR results presented the same 222632237401435 patterns. The molecular investigation uncovered information on the relationship of Mycobacterium bovis. Full article
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