Recent Advances in Nontuberculous Mycobacteria (NTM)—2nd Edition

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Bacterial Pathogens".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 825

Special Issue Editors


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Guest Editor
Department of Microbiology, National Tuberculosis Reference Laboratory, National Tuberculosis and Lung Diseases Research Institute, Płocka 26, 01-138 Warsaw, Poland
Interests: clinical microbiology; genotyping of Mycobacterium tuberculosis complex strains; TB transmission; Mycobacterium drug resistance
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E-Mail Website
Guest Editor
Department of Microbiology, National Tuberculosis Reference Laboratory, National Tuberculosis and Lung Diseases Research Institute, Płocka 26, 01-138 Warsaw, Poland
Interests: clinical microbiology; microbiological diagnostics of tuberculosis and mycobacteriosis
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Nontuberculous mycobacteria (NTM) constitute a group of over 180 Mycobacterium species, with the exception of the Mycobacterium tuberculosis complex and Mycobacterium leprae. NTM species can cause a variety of infections in humans and animals, including pneumonia, lung abscess, pleural infection, meningitis, lymphadenitis, and skin and soft tissue infection. Mycobacterioses are difficult to treat because the therapy is long, expensive, more toxic and prone to failure than tuberculosis treatment. Recently, a global increase in the number of infections caused by NTM bacilli has been recorded.

All NTM species are acid-fast; therefore, the staining of microscopic smears using fluorescence and the Ziehl–Neelsen method is not sufficient for species identification or distinguishing NTM from MTBC species. The proper diagnosis of mycobacteriosis is based on clinical features and microbiological tests, including culture, histopathology and molecular methods. Knowing the capabilities and limitations of laboratory testing is critical to making the right clinical decisions. Hence, among the most important tasks of modern microbiology laboratories are the diagnosis and species identification of NTM, while also distinguishing them from MTBC strains.

In this Special Issue, we will provide an overview of the current diagnostic options for suspected NTM infection.

Dr. Monika Kozińska
Prof. Dr. Ewa Augustynowicz-Kopeć
Guest Editors

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Keywords

  • mycobacteriosis
  • identification
  • nontuberculous mycobacteria (NTM)
  • mycobacteria other than tuberculosis (MOTT)
  • epidemiology
  • Mycobacterium spp.
  • microbiological diagnostics

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Published Papers (1 paper)

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Research

10 pages, 541 KiB  
Article
Evaluation of the Standard M10 MTB/NTM Molecular Test for the Rapid Identification of Tuberculous and Nontuberculous Mycobacteria in Liquid Cultures
by Sara Caldrer, Alberta Carrara, Andrea Ragusa, Lavinia Nicolini, Elena Pomari, Cristina Mazzi, Fabio Formenti and Francesca Perandin
Pathogens 2025, 14(6), 517; https://doi.org/10.3390/pathogens14060517 - 22 May 2025
Viewed by 554
Abstract
Since 2013, the World Health Organization has recommended the use of rapid molecular tests as the initial diagnostic step for Mycobacterium tuberculosis (MTB) infection to enhance the control of tuberculosis. In recent years, the prevalence of infections by non-tuberculous mycobacteria (NTM) in humans [...] Read more.
Since 2013, the World Health Organization has recommended the use of rapid molecular tests as the initial diagnostic step for Mycobacterium tuberculosis (MTB) infection to enhance the control of tuberculosis. In recent years, the prevalence of infections by non-tuberculous mycobacteria (NTM) in humans has also risen, particularly in countries with low tuberculosis incidence, such as Italy. Therefore, the rapid differentiation between NTM and Mycobacterium tuberculosis complex is crucial for timely therapeutic decisions. This study evaluates a new rapid molecular assay, Standard M10 MTB/NTM, designed to detect MTB, NTM, or co-detection in Mycobacteria Growth Indicator Tube cultures from different biological matrices. The assay was validated using 100 positive and 50 negative liquid mycobacteria cultures, already confirmed by specific real-time PCR and Sanger sequencing. Following optimization of assay conditions for culture sample processing and assessment of potential interference, Standard M10 demonstrated excellent sample stability, high specificity, and good sensitivity, identifying all 50 MTB and 49 NTM samples. Some limitations included the non-detection of M. celatum in one case and false positive results (MTB co-infection) in two NTB cases. Nevertheless, overall, the adoption of this test could be considered for laboratory management to enable rapid and effective sample targeting for subsequent diagnostic evaluation and treatment decision-making. Full article
(This article belongs to the Special Issue Recent Advances in Nontuberculous Mycobacteria (NTM)—2nd Edition)
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