Contemporary Health Issues of Aquatic Organisms-Special Issue Dedicated to Dr. Rocco Cipriano

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Veterinary Microbiology".

Deadline for manuscript submissions: 31 July 2026 | Viewed by 1921

Special Issue Editors


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Guest Editor
Department of Pathobiology and Diagnostic Investigation, College of Veterinary Medicine and Department of Fisheries and Wildlife, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI, USA
Interests: diseases of aquatic animals; host-pathogen interaction; diagnosis of diseases; pathogenic mechanisms of infectious diseases
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Aquatic Animal Health Laboratory, Aquatic Animal Disease Ecology Program, Michigan State University, East Lansing, MI 48824, USA
Interests: aquatic animal health; pathogenesis, ecology, and epidemiology of infectious aquatic animal diseases; microbiology

Special Issue Information

Dear Colleagues,

We are humbled for the opportunity to be the Guest Editor of this Special Issue in the highly regarded journal Microorganisms dedicated to Dr. Rocco C. Cipriano of the United States Geological Service, Leetown Science Center-Fish Health Laboratory, Leetown, West Virginia. Dr. Cipriano dedicated his very productive career to studying diseases of aquatic animals, developing enhanced diagnostics, and designing biosecurity strategies to mitigate their effects. His passion to go beyond political borders to gather aquatic animal health professionals from allover the world created an unparallel platform to exchange ideas and experiences and was an inspiration to scientists, seniors, and juniors alike. We, his students, friends, colleagues, and collaborators, are assembling this Special Issue on emerging infectious diseases of aquatic organisms as a tribute to Dr. Cipriano outstanding career.

As you are aware, all aquatic organisms, regardless of their taxonomic affiliations, have recently been faced with challenges of unprecedented magnitude that can threaten their existence. Among these challenges, ongoing climate change occupies a central position due to its cascading physical, chemical, and biological effects. Such adverse effects may facilitate the emergence of novel pathogens and compromise host defense mechanisms thereby leading to devastating ecological and economic consequences. Antimicrobial resistant strains, the white spot syndrome virus in shrimp, Perkinsosis in shellfish, bleaching of coral organisms, tilapia lake virus of cichlids, chytridiomycosis of amphibians, paramyxoviruses of marine mammals, and wasting disease of sea grass, are just examples of emerging diseases and pathogens that have devastated communities of aquatic organisms worldwide. Though not proven, climate change, habitat loss, anthropogenic activities, trade of live organisms, and aquaculture practices have contributed to the emergence of novel pathogens and resurgence of old ones. To face these challenges, scientists are encouraged to unravel the underlying mechanisms of pathogen emergence and develop adaptive strategies that managers implement for conserving aquatic communities at risk.

This Special Issue with your valuable collection of research papers and critical reviews and case studies will provide a panoramic platform through which scientists and managers communicate their research and strategies for prevention and control of emerging pathogens under the One Health concept. Your manuscripts addressing prokaryote and eucaryote disease ecology, conservation medicine, epidemiology, and underlying molecular mechanisms of host-pathogen interactions will fill an important gap of knowledge.

Dr. Mohamed Faisal
Dr. Thomas Loch
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

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Keywords

  • antimicrobial resistance
  • emerging pathogens of aquatic organisms
  • epidemiology
  • pathogenesis
  • immunology
  • disease ecology
  • role of climatic change in pathogen emergence
  • prevention and control
  • vaccine
  • metagenomic and metatranscriptomic analyses

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Published Papers (2 papers)

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Research

22 pages, 2731 KB  
Article
Phylogenetic and Proteomic Analyses of Segment 2 Sequence Reveals the Presence of Two Variants of a Divergent Amnoonvirus (Family: Amnoonviridae) Infecting Nile Tilapia (Oreochromis niloticus)
by Amel M. El Asely, Mohamed Shawky Khalifa, Wei Xu, Adel A. Shaheen and Mohamed Faisal
Microorganisms 2026, 14(2), 343; https://doi.org/10.3390/microorganisms14020343 - 2 Feb 2026
Viewed by 623
Abstract
Illumina sequencing of segment 2, which encodes the polymerase basic subunit 2 (PB2) of the RNA-dependent RNA polymerase of a divergent amnoonvirus recently detected in tissues of Nile tilapia farmed in Egypt, revealed the presence of two genetic variants of the same virus: [...] Read more.
Illumina sequencing of segment 2, which encodes the polymerase basic subunit 2 (PB2) of the RNA-dependent RNA polymerase of a divergent amnoonvirus recently detected in tissues of Nile tilapia farmed in Egypt, revealed the presence of two genetic variants of the same virus: AmnoonvirusEGY1F and -H. The phylogenetic and genetic analyses presented in this study support the inclusion of both variants in the genus Tilapinevirus, family Amnoonviridae, order Articulavirales. The Egyptian strains formed distinct, well-supported clades in both nucleotide- and amino acid-based trees, showing a notable divergence from unclassified amnoonviruses and clustered with members of the genus Tilapinevirus. Within the genus Tilapinevirus, both Egyptian strains were divergent from all tilapia lake virus (TiLV) strains, whose full RNA segment 2 sequences are available in public databases, as well as the newly isolated Tilapinevirus poikilos from the fancy-tailed guppy. The Egyptian strains were also divergent from TiLV strains identified in Israel and Lake Victoria. Although the PB2 proteins of AmnoonvirusEGY1F and -H exhibited striking similarity, several mutations were detected that altered the sequence of their antigenic cell epitopes. Some of these mutations in the AmnoonvirusEGY1H strain were predicted to affect PB2-encoded functions. Collectively, findings of this study aid in the growing understanding of viral diversity and PB2 evolution in emerging amnoonviruses, particularly the role of amino acid substitutions in affecting the encoded protein structure, function, and immunogenicity. Full article
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17 pages, 2568 KB  
Article
Plesiomonas shigelloides as an Emerging Pathogen in Catfish Aquaculture: A Case from a South Texas Commercial Farm
by Haitham H. Mohammed, Noha I. ElBanna, Ozgur Erdogan, Suja Aarattuthodi, Hasan C. Tekedar, Hossam Abdelhamed and Josué Díaz-Delgado
Microorganisms 2026, 14(1), 144; https://doi.org/10.3390/microorganisms14010144 - 8 Jan 2026
Viewed by 901
Abstract
During the summer of 2023, a spontaneous disease outbreak occurred in intensively stocked hybrid catfish (♀ channel catfish, Ictalurus punctatus × ♂ blue catfish, I. furcatus) in earthen ponds on a commercial aquaculture farm in South Texas. The farmer reported 50 to [...] Read more.
During the summer of 2023, a spontaneous disease outbreak occurred in intensively stocked hybrid catfish (♀ channel catfish, Ictalurus punctatus × ♂ blue catfish, I. furcatus) in earthen ponds on a commercial aquaculture farm in South Texas. The farmer reported 50 to 80 dead fish per pond daily for a month. The fish were market size (1.0 ± 0.3 kg on average), resulting in substantial economic losses. Fifteen moribund fish were submitted for laboratory examination. Grossly, the fish showed distended abdomens, erythematous fins, and inflamed vents. Autopsy demonstrated visceral congestion, distended gastrointestinal tracts, and serosanguineous peritoneal effusion. Bacterial cultures from the internal organs revealed homogeneous bacterial growth after incubation. Presumptive biochemical characterization of the isolated bacteria identified Plesiomonas shigelloides. Further molecular confirmation was achieved by species-specific PCR amplification and 16S-rRNA sequencing. Juvenile catfish were experimentally challenged with the recovered isolates to fulfill Koch’s postulates. Moreover, an antibiogram was performed to evaluate the susceptibility of the isolates to a panel of FDA-approved antimicrobials. P. shigelloides isolates were pathogenic to channel catfish and alarmingly multidrug-resistant. We report here, for the first time, P. shigelloides infection in Texas commercial catfish aquaculture, emphasizing its significance as an emerging enteric pathogen that is difficult to treat with FDA-approved antimicrobials. Full article
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