Novel Strategies in the Research of the Human Gut Microbiota

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Gut Microbiota".

Deadline for manuscript submissions: closed (31 October 2025) | Viewed by 822

Special Issue Editor


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Guest Editor
Centro de Investigacion y de Estudios Avanzados del Instituto Politécnico Nacional, Mexico City, Mexico
Interests: transcription factor 7; single nucleotide polymorphism; gestational diabetes

Special Issue Information

Dear Colleagues,

The human gut microbiota is a complex system of microorganisms that live mainly in the colon. The aim of this Special Issue is to provide a collection of articles on microbiota composition and human health. I invite you to submit research articles, review articles, and short communications related to novel strategies in the research of the human gut microbiota.

Dr. Carlos Hoyos Vadillo
Guest Editor

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Keywords

  • gut
  • microbiota
  • human health

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Published Papers (1 paper)

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Research

19 pages, 1979 KB  
Article
The Effect of Cefazolin on the Gut Microbiome of Female Rats After Spinal Cord Injury
by Luis H. Pagán-Rivera, Filipa Godoy-Vitorino, Natalie M. Meléndez-Vázquez, Samuel E. Ocasio-Rivera, María E. Santiago-Gascot, Jose M. Santiago, Iris Salgado, Viviana González, Osmarie Martínez-Guzmán, Mauricio Cáceres-Chacón, Aranza Torrado-Tapias and Jorge D. Miranda
Microorganisms 2025, 13(10), 2324; https://doi.org/10.3390/microorganisms13102324 - 7 Oct 2025
Viewed by 709
Abstract
Spinal cord injury (SCI) is a devastating neurological state that could lead to motor, sensory, and autonomic dysfunction. In addition to its direct impact on the central nervous system, SCI exerts systemic effects, including disruption of gut homeostasis and alterations in the gut [...] Read more.
Spinal cord injury (SCI) is a devastating neurological state that could lead to motor, sensory, and autonomic dysfunction. In addition to its direct impact on the central nervous system, SCI exerts systemic effects, including disruption of gut homeostasis and alterations in the gut microbiota, which can contribute to sustained inflammation and hinder functional recovery. While antibiotic administration during the acute phase of SCI is clinically indicated, it may exacerbate microbial dysbiosis. In this study, we investigate the combined effects of SCI and cefazolin treatment on the gut microbiome of female rats. Animals were assigned to three groups: NAÏVE (no intervention), SHAM (cefazolin only), and INJURY (T10 spinal cord contusion plus cefazolin). Cefazolin was administered for seven days after the injury, fecal samples were collected at baseline (day 0), and on days 7, 14, 21, and 28 post-SCI. DNA was extracted and subjected to 16S rRNA gene amplicon sequencing, followed by bioinformatic analysis. Our findings revealed significant microbial dysbiosis in the INJURY group, including reduced alpha diversity and distinct shifts in microbial composition. These changes were most prominent during the acute phase post-SCI. Our findings highlight the compounding effects of spinal trauma and antibiotic exposure on the gut microbiome and suggest that maintaining microbial stability may represent a promising avenue to support recovery after SCI. Full article
(This article belongs to the Special Issue Novel Strategies in the Research of the Human Gut Microbiota)
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