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It is estimated that 30% of diseases caused by mutations can be linked to splicing. Mutation of a splice site can result in site loss of function and specificity, causing insertion or deletion of aminoacids or a disruption of the reading frame. The importance of understanding and properly decoding roles governing splicing have become a fact for the research community as demonstarted by the increasing number of manuscripts devoted to this subject. Splicing 2016 intends to join in a friendly academic environment all of us working or interested in moving to this area of research.