Special Issue "CRISPR-Cas: Interactions with Genome and Physiological Maintenance"

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Molecular Genetics and Genomics".

Deadline for manuscript submissions: 15 November 2019.

Special Issue Editors

Dr. Ed Bolt
E-Mail Website1 Website2
Guest Editor
The University of Nottingham Medical School, Queen's Medical Centre, Nottingham NG7 2UH, UK
Interests: molecular biology; protein biochemistry; CRISPR-cas immunity; homologous recombination; DNA Repair
Dr. Christian Rudolph
E-Mail Website1 Website2
Guest Editor
Department of Life Sciences, Brunel University, London, UK
Interests: cell biology, DNA replication, replication termination, chromosome dynamics
Dr. Ivana Ivancic-Bace
E-Mail Website
Guest Editor
Horvatovac 102a, Department of Biology, University of Zagreb, 10000 Zagreb, Croatia
Interests: Genetic analysis; CRISPR-Cas immunity; homologous recombination; DNA repair

Special Issue Information

Dear Colleagues,

CRISPR-Cas enzymes provide a growing smorgasbord of tools for genetic engineering of DNA and RNA, and in genome editing to alter cell physiology in bacteria, plants and mammals. Interactions between CRISPR-Cas and host DNA repair enzymes are important for successful genome editing because editing enzymes generate DNA damage sites. These trigger DNA repair systems and can provoke wider genomic stress with potential to disrupt DNA replication and cell cycle progression. In their native cells CRISPR-Cas adaptive immunity systems functionally interact with DNA repair and genome stability systems, factors that promote building of the DNA-based CRISPR immunity system. CRISPR-Cas enzymes also impact on other physiological systems in interesting ways by mechanisms unknown, for example in bacterial biofilm formation. Understanding interplay between CRISPR-Cas enzymes and other host physiologies is a frontier for improving efficacy of gene-editing protocols, furthering understanding of DNA repair in healthcare, and for understanding prokaryotic biology. This collection of articles will evaluate current knowledge, for example:

  • Developments in understanding off-target effects of class II CRISPR-Cas systems; Cas9, Cas12a, Cas13a.
  • Interactions of DNA repair, replication and recombination with native CRISPR-Cas systems.
  • Interactions of DNA repair, replication and recombination with heterologous CRISPR-Cas systems used in genome editing.
  • Genome editing in plants.
  • Genome editing for growth, sustainability and development projects and in human disease.
  • DNA editing using adaptation enzymes Cas1-Cas2.
  • Class III CRISPR systems and cell signaling.
  • Class I CRISPR systems and biofilm formation.

Dr. Ed Bolt
Dr. Christian Rudolph
Dr. Ivana Ivancic-Bace
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • CRISPR-Cas
  • DNA repair
  • genome editing
  • bacterial physiology
  • microbiology
  • homologous recombination
  • helicases
  • nucleases

Published Papers (1 paper)

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Research

Open AccessArticle
The Effect of DNA Topology on Observed Rates of R-Loop Formation and DNA Strand Cleavage by CRISPR Cas12a
Genes 2019, 10(2), 169; https://doi.org/10.3390/genes10020169 - 22 Feb 2019
Cited by 2
Abstract
Here we explored the mechanism of R-loop formation and DNA cleavage by type V CRISPR Cas12a (formerly known as Cpf1). We first used a single-molecule magnetic tweezers (MT) assay to show that R-loop formation by Lachnospiraceae bacterium ND2006 Cas12a is significantly enhanced by [...] Read more.
Here we explored the mechanism of R-loop formation and DNA cleavage by type V CRISPR Cas12a (formerly known as Cpf1). We first used a single-molecule magnetic tweezers (MT) assay to show that R-loop formation by Lachnospiraceae bacterium ND2006 Cas12a is significantly enhanced by negative DNA supercoiling, as observed previously with Streptococcus thermophilus DGCC7710 CRISPR3 Cas9. Consistent with the MT data, the apparent rate of cleavage of supercoiled plasmid DNA was observed to be >50-fold faster than the apparent rates for linear DNA or nicked circular DNA because of topology-dependent differences in R-loop formation kinetics. Taking the differences into account, the cleavage data for all substrates can be fitted with the same apparent rate constants for the two strand-cleavage steps, with the first event >15-fold faster than the second. By independently following the ensemble cleavage of the non-target strand (NTS) and target strand (TS), we could show that the faster rate is due to NTS cleavage, the slower rate due to TS cleavage, as expected from previous studies. Full article
(This article belongs to the Special Issue CRISPR-Cas: Interactions with Genome and Physiological Maintenance)
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