Omics Research in Dairy Sciences

A special issue of Dairy (ISSN 2624-862X). This special issue belongs to the section "Dairy Systems Biology".

Deadline for manuscript submissions: closed (30 November 2022) | Viewed by 2562

Special Issue Editors


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Guest Editor
Institute of Medical Biochemistry, University of Veterinary Medicine Vienna, 1210 Vienna, Austria
Interests: proteomics; two-dimensional electrophoresis; DIGE; animal proteins

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Guest Editor
LEAF—Linking Landscape, Environment, Agriculture and Food Research Center, Associated Laboratory TERRA, Instituto Superior de Agronomia, Universidade de Lisboa, Tapada, Lisboa, Portugal
Interests: animal and dairy science; proteomics as a tool in animal science research
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
USDA / ARS / National Animal Disease Center, Washington, DC, USA
Interests: proteomics; mastitis; pathogensis; immune function

Special Issue Information

Dear Colleagues,

Omics technologies, specifically proteomics and metabolomics, have become important tools during the last decade, also in the field of animal research, animal health, and product quality. In this Special Issue, we want to span a wide range of aspects, all illustrating the benefits and achievements of applying omics and systems biology in dairy science, including animal health (including metabolic diseases and mastitis, but also reproduction) and welfare and their influence on milk quality; the characterization of milk and dairy products and ensuring their quality; the influence of nutrition; compositional changes and influence on offspring or consumer; and region-specific dairy products and specialities. The Special Issue is directed to all dairy species, i.e., cattle, sheep, goat, and water buffalo; however, it will also be open to investigations on milk of non-ruminants if covering new aspects.

Thus, we strongly encourage the submission of review articles, research papers, and technical notes dealing with omics in all aspects related to dairy sciences.

Dr. Ingrid Miller
Dr. Andre M. Almeida
Dr. John Lippolis
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Dairy is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • milk and dairy products
  • species differences
  • mastitis
  • animal welfare
  • reproduction
  • glycosylation
  • lipidomics
  • vesicles
  • bioactive peptides
  • immunology
  • allergenicity

Published Papers (1 paper)

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Research

17 pages, 3559 KiB  
Article
The Impact of Low-Temperature Inactivation of Protease AprX from Pseudomonas on Its Proteolytic Capacity and Specificity: A Peptidomic Study
by Chunyue Zhang, Sjef Boeren, Liming Zhao, Etske Bijl and Kasper Hettinga
Dairy 2023, 4(1), 150-166; https://doi.org/10.3390/dairy4010011 - 3 Feb 2023
Viewed by 1924
Abstract
The destabilization of UHT milk during its shelf life can be promoted by the residual proteolytic activity attributed to the protease AprX from Pseudomonas. To better understand the hydrolysis patterns of AprX, and to evaluate the feasibility of using low-temperature inactivation (LTI) [...] Read more.
The destabilization of UHT milk during its shelf life can be promoted by the residual proteolytic activity attributed to the protease AprX from Pseudomonas. To better understand the hydrolysis patterns of AprX, and to evaluate the feasibility of using low-temperature inactivation (LTI) for AprX, the release of peptides through AprX activity on milk proteins was examined using an LC-MS/MS-based peptidomic analysis. Milk samples were either directly incubated to be hydrolyzed by AprX, or preheated under LTI conditions (60 °C for 15 min) and then incubated. Peptides and parent proteins (the proteins from which the peptides originated) were identified and quantified. The peptides were mapped and the cleavage frequency of amino acids in the P1/P1′ positions was analyzed, after which the influence of LTI and the potential bitterness of the formed peptides were determined. Our results showed that a total of 2488 peptides were identified from 48 parent proteins, with the most abundant peptides originating from κ-casein and β-casein. AprX may also non-specifically hydrolyze other proteins in milk. Except for decreasing the bitterness potential in skim UHT milk, LTI did not significantly reduce the AprX-induced hydrolysis of milk proteins. Therefore, the inactivation of AprX by LTI may not be feasible in UHT milk production. Full article
(This article belongs to the Special Issue Omics Research in Dairy Sciences)
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