Advanced Optical Imaging Technologies and Fluorescent Probes

A special issue of Chemosensors (ISSN 2227-9040). This special issue belongs to the section "Optical Chemical Sensors".

Deadline for manuscript submissions: 30 November 2026 | Viewed by 156

Special Issue Editor


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Guest Editor
School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China
Interests: organic luminescent dyes; fluorescent probe; biomedical imaging

Special Issue Information

Dear Colleagues,

Optical imaging technology continues to revolutionize our ability to visualize and understand biological processes in real time at, enabling us to acquire critical insights into disease mechanisms, diagnostics, and therapeutic monitoring. Many advanced imaging modalities (e.g., super-resolution microscopy, two-photon imaging, photoacoustic imaging and fluorescence lifetime imaging microscopy) have recently emerged, driven by breakthroughs with regard to powerful molecular probes. However, major challenges remain in terms of improving sensitivity, selectivity, biocompatibility and clinical translation.

The Special Issue will provide a forum for the latest research activities in the field of optical imaging technologies and molecular probes and their applications. We welcome both review articles and original research papers in areas including, but not limited to, the following:

  1. Small molecular fluorescent probes;
  2. Nano-fluorescent probes;
  3. Macromolecular fluorescent probes;
  4. NIR-I and NIR-II fluorescence imaging;
  5. Photoacoustic imaging;
  6. Fluorescence lifetime imaging microscopy;
  7. Biomedical imaging applications of probes.

Prof. Dr. Baoli Dong
Guest Editor

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Keywords

  • fluorescent probes
  • living cell imaging
  • in vivo imaging
  • biomedical imaging applications

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Published Papers (1 paper)

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Review

21 pages, 4971 KB  
Review
Fluorogenic Probe-Coupled Single-Molecule Fluorescence Imaging for Photocatalytic Mechanism Research
by Zeqi Yu, Xinyu Sun, Yanan Niu, Chaoyu Song, Yukang Sun and Yuguang Lv
Chemosensors 2026, 14(6), 126; https://doi.org/10.3390/chemosensors14060126 - 1 Jun 2026
Abstract
Elucidating structure–activity relationships in semiconductor photocatalysis has been significantly impeded by the inherent limitations of ensemble-averaged characterization techniques, which obscure the spatiotemporal heterogeneity intrinsic to catalytic surfaces. Single-molecule fluorescence microscopy (SMFM) surmounts this bottleneck by offering nanometer-scale spatial resolution coupled with the capacity [...] Read more.
Elucidating structure–activity relationships in semiconductor photocatalysis has been significantly impeded by the inherent limitations of ensemble-averaged characterization techniques, which obscure the spatiotemporal heterogeneity intrinsic to catalytic surfaces. Single-molecule fluorescence microscopy (SMFM) surmounts this bottleneck by offering nanometer-scale spatial resolution coupled with the capacity to resolve single-turnover events. Herein, we provide a comprehensive overview of the State-of-the-Art applications of fluorogenic probe-coupled SMFM in deciphering the microscopic mechanisms governing photocatalysis. We begin by delineating the operational principles of total internal reflection fluorescence (TIRF) microscopy and categorizing the response mechanisms of three distinct classes of fluorogenic probes: oxidative (e.g., Amplex Red, APF), reductive (e.g., Resazurin, DN-BODIPY), and acidic (e.g., furfuryl alcohol, thiophene) reporters. Subsequently, we highlight seminal studies wherein SMFM has been leveraged to visualize facet-dependent charge separation on model photocatalysts—including TiO2, BiOBr, and InSe—to map the dynamic activity associated with surface defects and to precisely locate active sites during photoelectrochemical water splitting. Finally, we critically assess the prevailing technical challenges, such as limitations in probe specificity and background interference, while offering a perspective on prospective avenues for methodological refinement. This review is intended to serve as a methodological cornerstone for advancing mechanistic understanding in photocatalysis and for guiding the rational design of high-performance catalysts. Full article
(This article belongs to the Special Issue Advanced Optical Imaging Technologies and Fluorescent Probes)
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