Live Cell Microscopy
A special issue of Biophysica (ISSN 2673-4125).
Deadline for manuscript submissions: 30 June 2025 | Viewed by 342
Special Issue Editors
Interests: biomedical optics; laser; microscopy
Special Issues, Collections and Topics in MDPI journals
2. Kirchhoff Institute for Physics, University Heidelberg, Heidelberg, Germany
Interests: advanced light microscopy methods to elucidate biological nanostructures; biophysics of the cell nucleus as the seat of genetic information and of gene regulation; development & biomedical applications of super-resolution methods to overcome the limits of conventional light microscopy, down to the nanometer range of optical and structural resolution
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
Light microscopy has emerged as one of the fundamental methods to analyse biological systems; applying super-resolution microscopy (SRM), an optical resolution down to the sub-nanometer range has recently been realized. However, most of these achievements have been made with fixed specimens, i.e. direct information about the dynamics of the biosystem studied was not possible. This stimulated the development of live cell microscopy approaches, e.g. Low Illumination Fluorescence Microscopy, Light Sheet Microscopy (LSM), Structured Illumination Microscopy (SIM), Fluorescence Correlation Spectroscopy (FCS), or Single Particle Tracking. This Special Issue is intended to provide an overview of recent developments in this field, as well as further perspectives, e.g. in terms of experimental improvements, or in the digital analysis of the typically huge data sets. The special issue includes preparation techniques of 2D and 3D specimens as well as various methods of transillumination, scattering and fluorescence microscopy. Label-free methods, e.g. phase or interference microscopy, Raman or autofluorescence microscopy may be addressed as well as the application of fluorescent probes or proteins. A further topic are micromanipulation techniques, e.g. laser tweezers or laser-assisted optoporation.
Prof. Dr. Herbert Schneckenburger
Prof. Dr. Christoph Cremer
Guest Editors
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Keywords
- living cells
- low light exposure
- 3D microscopy
- super-resolution
- laser micromanipulation
- image acquisition and processing
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