Stem Cell Therapy and Tissue Engineering

A special issue of Biomedicines (ISSN 2227-9059). This special issue belongs to the section "Cell Biology and Pathology".

Deadline for manuscript submissions: 31 May 2026 | Viewed by 1186

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Guest Editor
1. Department of Bioengineering, U.A. Whitaker College of Engineering, Florida Gulf Coast University, 10501 FGCU Boulevard South, Fort Myers, FL 33965, USA
2. Department of Chemistry, Faculty of Sciences, Canakkale Onsekiz Mart University, Terzioglu Campus, 17100 Canakkale, Turkey
Interests: natural polymers; glycosaminoglycans; sugar alcohols; carbohydrates; polyphenolics; amino acids; peptides; nano/microparticles; antioxidant; anti-inflammatory; antimicrobial activity; ROS-scavenging effect; antiviral; antifungal; biocidal; anticancer; controlled release; drug delivery; biocompatible; blood compatible; surface; (bio)interfaces; smart surfaces; surface modification; superhydrophobic; super hydrophilic; (bio)sensor; theragnostic, biopolymeric MRI agents; injectables biopolymeric materials; hydrogel; cryogel; microgel; nanogels; super porous materials; pharmacology; wound healing; wound dressing; medical device; tissue engineering; stimuli-responsive structures; wearables; artificial skin; shape memory polymers; injectable materials; biodegradable active agent carriers; carbon particle; quantum dots, carbon dots, graphene dots, g-C3N4; lubricant, intra-articular and joints injection materials; cosmetics
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Special Issue Information

Dear Colleagues,

Recent developments in stem cell therapy (SCT) or regenerative medicine, especially in the fields of various chronic and acute disease treatments and organs associated with human health, are steadily increasing. It has been accepted that the therapeutic utilization of stem cells (SCs) or their derivatives not only enhances the repair and/or replaces the malfunctioned or damaged tissue but also improves quality of life. SCs can be engineered into specific types of cells and organs, e.g., skin cells, blood cells, nerve cells, muscle cells and so on, that can be implanted to contribute to the repair of damaged tissue and organs. SCT application is not limited to cardiovascular, ophthalmological, neurological, orthopedical, and dental therapy, but is also used in cancer therapy as well as in testing new drugs. ALthough there are many types of SCs, the two main types depending on their differential potential are pluripotent stem cells (PSCs), which are embryonic SCs and induced pluripotent SCs, and tissue-specific (adult) SCs, which are also considered multipotent SCs and can only differentiate into specific cell types within their tissue of origin.

The materials employed in SC research and therapy are a critical aspect of tissue engineering. These materials can be synthetic or natural in origin or a combination of both or composites with some inorganic components, biocompatible and/or biodegradable templates, or scaffolds that host the SCs and the required essential additives. These materials are generally hydrogels, cryogels, and porous networks that can be derived from a variety of biomaterials affording structural support and mimicking the extracellular matrix. The presence of growth factors, small proteins (cytokines), and other additives along with SCs within tissue engineering materials can steer and guide the differentiation and proliferation of SCs into the desired cell types. Therefore, advanced versatile biomaterials for tissue engineering applications of SCs are of paramount significance for the success of SCT.

This Special Issue primarily focuses on SC proliferation and differentiation for specific cell development and production within biomaterials used as scaffolds, and the nature and importance of these templates used in SC tissue engineering will be emphasized. The SCT for the treatment of various diseases, cancer therapy, and drug testing are within the scope of this Special Issue.

Dr. Nurettin Sahiner
Guest Editor

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Keywords

  • stem cells
  • stem cell therapy
  • stem cell cancer therapy
  • hydrogels/cryogels
  • porous network biomaterials
  • tissue engineering

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Published Papers (1 paper)

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Research

20 pages, 3596 KB  
Article
Effect of Allogenic Mesenchymal Stem Cell Injection on Functional Repair Outcomes Following Skeletal Muscle Laceration Injury
by Raja Elina Ahmad, Abdul Halim Mokhtar, Mohamed Zubair Mohamed Al-Fayyadh, Hui Yin Nam, Atiqah Aziz, Azura Mansor and Tunku Kamarul
Biomedicines 2025, 13(11), 2810; https://doi.org/10.3390/biomedicines13112810 - 18 Nov 2025
Viewed by 832
Abstract
Background: Skeletal muscle laceration injuries remain a clinical challenge owing to limited and often delayed functional recovery. Surgical repair often fails to fully restore injured muscle, causing fibrosis and functional impairments. Mesenchymal stem cells (MSCs) represent a potential therapy due to their [...] Read more.
Background: Skeletal muscle laceration injuries remain a clinical challenge owing to limited and often delayed functional recovery. Surgical repair often fails to fully restore injured muscle, causing fibrosis and functional impairments. Mesenchymal stem cells (MSCs) represent a potential therapy due to their regenerative and immunomodulatory properties. However, their short-term regenerative effects in laceration injuries remain under-explored. Objective: We aim to evaluate the short-term effects of allogenic bone marrow-derived MSCs on skeletal muscle regeneration following laceration injury in rats. Methods: Sprague Dawley rats underwent laceration injury to the right gastrocnemius muscle and received local injection of either saline (n = 6) or allogeneic bone marrow-derived MSCs (2 × 106 cells; n = 6) two weeks after injury. Muscle functional recovery was evaluated by measuring tetanic contraction force of the injured relative to the contralateral uninjured leg and compared among MSC-treated, saline-treated, untreated injured (n = 6), and intact control groups (n = 6) on days 7 and 14 post-treatment. Histological assessment of the treated muscle groups using Hematoxylin and Eosin and Masson’s Trichrome staining was conducted on day 7 post-treatment. Results: On day 7 post-treatment, MSC-treated muscle showed higher normalised force (96.8 ± 15.0%) than saline-treated (76.7 ± 4.6%) (p = 0.0393), but not untreated, muscle (83.1 ± 14.7%) (p = 0.2259). By day 14, the MSC-treated group exhibited significantly greater recovery of muscle force (110.8 ± 6.46%) than both the saline-treated (78.4 ± 6.47%) (p < 0.0001) and untreated groups (88.1 ± 3.41%) (p = 0.0001). Force recovery in the MSC-treated muscle was comparable to that in intact muscle (102.6 ± 10.4%) at both time points (p = 0.230). Supplementary histological analysis showed mild inflammatory cell infiltration, well-formed myoblasts, and a lower fibrosis index in MSC-treated muscle (29.30 ± 0.29%) compared with saline-treated muscle (31.77 ± 0.43%) (p < 0.0001) on day 7 post-treatment. Conclusions: Allogeneic bone marrow-derived MSC therapy is associated with enhanced repair of lacerated skeletal muscle over a short recovery period; however, larger studies with broader assessments are needed to confirm its potential clinical applicability. Full article
(This article belongs to the Special Issue Stem Cell Therapy and Tissue Engineering)
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