Special Issue "Human ECM in Homeostasis and Pathology"

A special issue of Biomedicines (ISSN 2227-9059). This special issue belongs to the section "Cell Biology and Pathology".

Deadline for manuscript submissions: 31 March 2023 | Viewed by 595

Special Issue Editors

Dr. Larisa M. Haupt
E-Mail Website
Guest Editor
Centre for Genomics and Personalised Health, Genomics Research Centre, School of Biomedical Sciences, Max Planck Queensland Centre, Queensland University of Technology (QUT), Queensland, 60 Musk Ave., Kelvin Grove, Brisbane, QLD 4059, Australia
Interests: extracellular matrix; proteoglycans; lineage specification; adult neurogenesis; microenvironment; initiation and progression of disease
Dr. Amaia Cipitria
E-Mail Website
Guest Editor
Ikerbasque Research Associate, Leader of the Bioengineering in Regeneration and Cancer Group, Biodonostia Health Research Institute, Paseo Doctor Beguiristain s/n, 200014 San Sebastián, Spain
Interests: biomaterials; extracellular matrix; tissue engineering; regenerative medicine; cancer and bone metastasis

Special Issue Information

Dear Colleagues,

It is well established that complex intercellular interactions, as well as biomolecular and biomechanical cues from the extracellular matrix, dictate the growth and maintenance of all tissues through bidirectional signaling, mediated by the physical properties of the ECM along with its biochemical composition. Many cell culture models, including ex vivo organoids, have improved our understanding of ECM development and homeostasis; however, they do not replicate the complex microenvironment of the native ECM across individual tissue types. This Special Issue aims to explore current efforts used to bridge this gap in knowledge across multiple disciplines, including cell biology, genomics, biomechanical and biophysical characterisation, 3D cell culture, and bioengineered smart materials.

Dr. Larisa M. Haupt
Dr. Amaia Cipitria
Guest Editors

Manuscript Submission Information

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  • extracellular matrix
  • biomechanical and biophysical characterisation
  • organoids
  • 3D culture
  • smart matrices
  • humanized models
  • proteoglycans

Published Papers (1 paper)

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Bioactive Cell-Derived ECM Scaffold Forms a Unique Cellular Microenvironment for Lung Tissue Engineering
Biomedicines 2022, 10(8), 1791; https://doi.org/10.3390/biomedicines10081791 - 26 Jul 2022
Cited by 1 | Viewed by 440
Chronic lung diseases are one of the leading causes of death worldwide. Lung transplantation is currently the only causal therapeutic for lung diseases, which is restricted to end-stage disease and limited by low access to donor lungs. Lung tissue engineering (LTE) is a [...] Read more.
Chronic lung diseases are one of the leading causes of death worldwide. Lung transplantation is currently the only causal therapeutic for lung diseases, which is restricted to end-stage disease and limited by low access to donor lungs. Lung tissue engineering (LTE) is a promising approach to regenerating a replacement for at least a part of the damaged lung tissue. Currently, lung regeneration is limited to a simplified local level (e.g., alveolar–capillary barrier) due to the sophisticated and complex structure and physiology of the lung. Here, we introduce an extracellular matrix (ECM)-integrated scaffold using a cellularization–decellularization–recellularization technique. This ECM-integrated scaffold was developed on our artificial co-polymeric BETA (biphasic elastic thin for air–liquid interface cell culture conditions) scaffold, which were initially populated with human lung fibroblasts (IMR90 cell line), as the main generator of ECM proteins. Due to the interconnected porous structure of the thin (<5 µm) BETA scaffold, the cells can grow on and infiltrate into the scaffold and deposit their own ECM. After a mild decellularization procedure, the ECM proteins remained on the scaffold, which now closely mimicked the cellular microenvironment of pulmonary cells more realistically than the plain artificial scaffolds. We assessed several decellularization methods and found that 20 mM NH4OH and 0.1% Triton X100 with subsequent DNase treatment completely removed the fibroblasts (from the first cellularization) and maintains collagen I and IV as the key ECM proteins on the scaffold. We also showed the repopulation of the primary fibroblast from human (without chronic lung disease (non-CLD) donors) and human bronchial epithelial (16HBE14o) cells on the ECM-integrated BETA scaffold. With this technique, we developed a biomimetic scaffold that can mimic both the physico-mechanical properties and the native microenvironment of the lung ECM. The results indicate the potential of the presented bioactive scaffold for LTE application. Full article
(This article belongs to the Special Issue Human ECM in Homeostasis and Pathology)
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