Molecular Biology of Cancer: From Biomarkers to Targeted Therapy

A special issue of Biomedicines (ISSN 2227-9059). This special issue belongs to the section "Cell Biology and Pathology".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 167

Special Issue Editor

Special Issue Information

Dear Colleagues,

Current research continues to unravel the molecular background of carcinogenesis and cancer progress. In particular, advances in molecular biology technologies contribute to better tumor profiling, thus paving the way for the investigation of novel, specific, prognostic, and predictive tumor biomarkers. These advances promote the development of new, personalized, and effective therapies, greatly contributing to improved patient outcomes in an era of precise medicine.

This Special Issue aims to present cutting-edge research focusing on the investigation of the molecular mechanisms involved in tumor evolution and progress, as well as exploring potential diagnostic, prognostic, or predictive biomarkers, working towards a more personalized molecular-based therapeutic intervention in cancer.

Original research articles and reviews in this field are welcome.

I look forward to receiving your contributions.

Dr. Georgia Levidou
Guest Editor

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Keywords

  • molecular biology
  • carcinogenesis
  • cancer
  • biomarkers
  • prognostic markers
  • predictive markers
  • precision medicine
  • targeted therapy

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Published Papers (1 paper)

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Research

22 pages, 1928 KB  
Article
Subtype-Independent Dysregulation of the Notch Signaling Pathway and Its miRNA Regulators in Breast Cancer
by Elżbieta Mitka-Krysiak, Katarzyna Król-Jatręga, Piotr Ossowski, Nikola Zmarzły, Krzysztof Bereza, Paweł Ordon, Wojciech Kulej, Tomasz Sirek, Agata Sirek, Kacper Boroń, Maciej Boroń, Dariusz Boroń and Beniamin Oskar Grabarek
Biomedicines 2025, 13(12), 3065; https://doi.org/10.3390/biomedicines13123065 - 12 Dec 2025
Abstract
Background/Objectives: The Notch signaling pathway regulates cell fate, proliferation, and differentiation, and its dysregulation has been implicated in various cancers, including breast cancer. MicroRNAs (miRNAs) are critical post-transcriptional regulators that can modulate Notch pathway components. The aim of this study was to [...] Read more.
Background/Objectives: The Notch signaling pathway regulates cell fate, proliferation, and differentiation, and its dysregulation has been implicated in various cancers, including breast cancer. MicroRNAs (miRNAs) are critical post-transcriptional regulators that can modulate Notch pathway components. The aim of this study was to identify miRNAs that may potentially regulate the expression of Notch pathway-related genes across five molecular subtypes of breast cancer in Polish women. Methods: Tumor and adjacent normal tissue samples were collected from 405 patients with five breast cancer subtypes: luminal A (n = 130), HER2-negative luminal B (n = 100), HER2-positive luminal B (n = 96), non-luminal HER2-positive (n = 36), and triple-negative breast cancer (n = 43). Gene expression was profiled using mRNA microarrays and validated with RT-qPCR and ELISA. Candidate regulatory miRNAs were identified by miRNA microarrays and confirmed using the miRDB database. Results: APH1A, CTBP1, DTX1, HEY1, HEY2, JAG2, NOTCH4, TLE2, and TLE4 were consistently dysregulated across all breast cancer subtypes. Overexpression of HEY1 and JAG2 may be driven by decreased levels of miR-145, miR-98, and miR-381. Conversely, downregulation of TLE4 may be associated with elevated expression of miR-196a and miR-155. No regulatory miRNAs meeting the selection criteria were identified for APH1A, CTBP1, DTX1, HEY2, NOTCH4, or TLE2. Conclusions: The consistent alterations suggest the presence of a shared Notch-driven oncogenic signature in breast cancer, potentially driving cell proliferation, stemness, and resistance to therapy. These findings enhance our understanding of Notch signaling in breast cancer and propose novel miRNA–Notch interactions as candidate targets for therapeutic intervention. Full article
(This article belongs to the Special Issue Molecular Biology of Cancer: From Biomarkers to Targeted Therapy)
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