Antibodies in Laboratory Diagnostic Techniques

A special issue of Antibodies (ISSN 2073-4468).

Deadline for manuscript submissions: closed (25 September 2024) | Viewed by 1998

Special Issue Editor

Special Issue Information

Dear Colleagues,

Laboratory diagnostics is currently a dynamically developing field of medical science. Modern laboratory techniques are very sensitive and specific. They can measure tens of thousands of identical substances, even at very low concentrations. This would not be possible without the use of diagnostic antibodies. In this Special Issue, we want to focus on current laboratory diagnostic techniques using diagnostic antibodies. Currently, there are no collected studies that cover this subject across a wide range. We also want to go back to the old days, when the introduction of immunodiagnostic methods to medical laboratories revolutionized modern laboratory diagnostics and opened the possibility for patients and doctors to diagnose various diseases by determining very specific markers of various pathologies using ultrasensitive laboratory tests. All this would not be possible without the use of an immunologist and diagnostic antibodies.

Dr. Kinga Lis
Guest Editor

Manuscript Submission Information

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Keywords

  • immunodiagnostics
  • immunochemistry
  • laboratory diagnostic

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Published Papers (1 paper)

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Research

12 pages, 1011 KB  
Article
Comparison of Antigen Conjugation to a Peptidic Carrier or to Bovine Serum Albumin in the Serodiagnosis of Canine Visceral Leishmaniasis via Suspension Array Technology
by Thais Stelzer Toledo, Pauline Martins Cunha, Josué da Costa Lima-Junior, Monique Paiva De Campos, Alinne R. S. Renzetti, Fabiano Borges Figueiredo, Fernanda Nazaré Morgado, Renato Porrozzi, Fatima da Conceição-Silva, Marta de Almeida Santiago and Paula Mello De Luca
Antibodies 2025, 14(4), 103; https://doi.org/10.3390/antib14040103 - 4 Dec 2025
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Abstract
Backgroud/Objectives: Canine Visceral Leishmaniasis (CVL), caused by Leishmania infantum, is a significant public health concern due to dogs serving as reservoirs for human infection. An accurate and rapid diagnostic method to distinguish symptomatic and asymptomatic CVL from healthy and vaccinated animals [...] Read more.
Backgroud/Objectives: Canine Visceral Leishmaniasis (CVL), caused by Leishmania infantum, is a significant public health concern due to dogs serving as reservoirs for human infection. An accurate and rapid diagnostic method to distinguish symptomatic and asymptomatic CVL from healthy and vaccinated animals is essential for controlling canine and human disease. Developing innovative antibody detection techniques and exploring new antigens are essential for enhancing CVL testing efficiency. Our study focuses on a multiplex flow cytometry technique to detect Leishmania-specific antibodies in canine serum. This involved conjugating small peptides with carrier proteins or peptide tags, sequences designed to facilitate bead coupling. Methods: A peptide from the L. infantum A2 protein was coupled to beads in three forms: unconjugated, conjugated with BSA, and conjugated with a C-terminal β-alanine–lysine (x4)–cysteine TAG. This TAG was previously designed to enhance peptide solubility, improve binding efficiency, and provide functional groups for covalent attachment to the beads, ensuring stable immobilization in the multiplex assay. Results: Our results suggest that the multiplex approach shows promise as a rapid serological test for CVL, particularly with TAG-conjugated peptides, which optimize bead coupling. However, peptide/BSA conjugation revealed anti-BSA antibodies in samples from healthy and CVL dogs. Conclusions: In conclusion, our findings highlight the potential of multiplex methodologies to enhance CVL diagnostics and caution against using BSA as a bead coupling agent in serological tests for canine samples due to its impact on test specificity and sensitivity. Full article
(This article belongs to the Special Issue Antibodies in Laboratory Diagnostic Techniques)
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