Proteomics for understanding disease condition in veterinary science

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Veterinary Clinical Studies".

Deadline for manuscript submissions: closed (28 February 2022) | Viewed by 3284

Special Issue Editor


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Guest Editor
Department of Animal Medicine and Surgery, BioVetMed research group, University of Murcia, 30100 Murcia, Spain

Special Issue Information

Proteomics is more than a technology for large-scale protein analysis. The first widely accepted use of proteomics was the identification of proteins from a cell, tissue, or body fluid. Nowadays, it may also be considered the optimal platform for biomarker discovery. However, changes in the amount of specific proteins may not always explain the studied condition, since post-translational modifications are critical for a protein's function. Therefore, the use of proteomics to identify the main post-translational protein modifications such as phosphorylation and glycosylation has become very important.

Proteomics is also the last step for the proper interpretation of gene expression differences by validation on the protein level. It is of great value for the functional annotation and determination of the enrichment of a set of proteins that could be altered in specific conditions.

The use of proteomics in veterinary science has exponentially increased in the last decade. On one hand, this increase has been produced by the use of animals to model human conditions. On the other hand, proteomics has been extensively applied in the analysis of animal products to improve protein-based foods. Moreover, the use of proteomics to examine animal health issues is also increasing. However, there is still a low number of studies that use proteomics techniques to understand veterinary diseases. This Special Issue is dedicated to proteomics in veterinary science, to better understand the host response to disease in the different veterinary species.

With the present Special Issue, we would like to provide updated knowledge about the possible proteomics protocols and tools that could be used to understand disease in veterinary science and offer examples about the interpretation of the data obtained

Dr. Ana María Gutiérrez
Guest Editor

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Keywords

  • Proteomics tools
  • animal science
  • disease condition
  • proteomics data
  • clinical use

Published Papers (1 paper)

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Research

14 pages, 12143 KiB  
Article
Serum Proteomic Changes in Dogs with Different Stages of Chronic Heart Failure
by Ahmet Saril, Meric Kocaturk, Kazumi Shimada, Akiko Uemura, Emel Akgün, Pinar Levent, Ahmet Tarik Baykal, Alberto Muñoz Prieto, Carlos Fernando Agudelo, Ryou Tanaka, Jose Joaquin Ceron, Jorgen Koch and Zeki Yilmaz
Animals 2022, 12(4), 490; https://doi.org/10.3390/ani12040490 - 16 Feb 2022
Cited by 6 | Viewed by 2719
Abstract
MMVD, the most common cause of CHF in dogs, is a chronic disease with variable clinical signs, with some patients remaining asymptomatic while others develop CHF. Here, we aimed to evaluate serum proteins by proteomic analysis in dogs at different stages of CHF [...] Read more.
MMVD, the most common cause of CHF in dogs, is a chronic disease with variable clinical signs, with some patients remaining asymptomatic while others develop CHF. Here, we aimed to evaluate serum proteins by proteomic analysis in dogs at different stages of CHF due to MMVD, and proteome behaviors after conventional treatment. A total of 32 dogs were divided equally into four groups—stage A (healthy/controls), stage B2 (asymptomatic), stage C and stage D (symptomatic)—according to the ACVIM consensus. Serum proteomes were evaluated using LC/MS-based label-free differential proteome analysis. The study revealed 157 different proteins; 11 were up- and 21 down-regulated in dogs with CHF compared to controls. In stage B2 dogs, angiotensinogen (AGT) was up-regulated, but immunoglobulin iota chain-like, lipopolysaccharide-binding protein, and carboxypeptidase (CPN) were down-regulated. In stage C dogs, complement C3 (C3) and inter-alpha-trypsin inhibitor heavy chain were up-regulated, but hemopexin, and actin-cytoplasmic-1 (ACT-1) were down-regulated. In stage D dogs, AGT was up-regulated, whereas tetranectin, paraoxonase-1, adiponectin and ACT-1 were down-regulated. A decrease in CPN, C3 and AGT and an increase in ACT-1 were observed after treatment of dogs in stage C. This pilot study identified that dogs at different stages of CHF show different serum protein composition which has potential to be biomarker for diagnose and treatment monitorization. Full article
(This article belongs to the Special Issue Proteomics for understanding disease condition in veterinary science)
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