Update on Role of Equine Microbiota in Health & Disease

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Equids".

Deadline for manuscript submissions: 30 June 2024 | Viewed by 1573

Special Issue Editor


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Guest Editor
School of Veterinary Medicine, The University of Surrey, Surrey, UK
Interests: equine

Special Issue Information

Dear Colleagues,

The equine gastrointestinal system and the bacteria that reside within it have always been inextricably linked with horse health. A better understanding of this relationship has the potential to refine clinical treatments for illness and improve horse welfare. With technologies that are often linked with analysing the equine microbiome continuously evolving, there is a constant steam of new insights into the relationship between these bacterial communities and equine illness. Therefore, there is a need to communicate new research within this area of equine research to both the wider research and clinical equine veterinary communities. We are pleased to invite the submissions of original research providing novel insights into the horse gut microbiome and how it is linked with horse health and/or disease to this Special Issue.

This Special Issue aims to update the equine research and clinical community on recent research exploring the link between the equine gut microbiome and horse health and disease. Original research articles and reviews are welcome. Research areas may include (but are not limited to) the following: equine gastroenterology, equine nutrition, equine behaviour and microbiology.

I look forward to receiving your contributions.

Dr. Joy Leng
Guest Editor

Manuscript Submission Information

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Keywords

  • equine
  • microbiome
  • DNA sequencing
  • gastrointestinal health
  • colic

Published Papers (1 paper)

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Research

18 pages, 1558 KiB  
Article
Homemade Nucleic Acid Preservation Buffer Proves Effective in Preserving the Equine Faecal Microbiota over Time at Ambient Temperatures
by Ashley B. Ward, Patricia A. Harris, Caroline McG. Argo, Christine Watson, Madalina Neacsu, Wendy R. Russell, Antonio Ribeiro, Elaina Collie-Duguid, Zeynab Heidari and Philippa K. Morrison
Animals 2023, 13(19), 3107; https://doi.org/10.3390/ani13193107 - 5 Oct 2023
Viewed by 1243
Abstract
The equine faecal microbiota is often assessed as a proxy of the microbial community in the distal colon, where the microbiome has been linked to states of health and disease in the horse. However, the microbial community structure may change over time if [...] Read more.
The equine faecal microbiota is often assessed as a proxy of the microbial community in the distal colon, where the microbiome has been linked to states of health and disease in the horse. However, the microbial community structure may change over time if samples are not adequately preserved. This study stored equine faecal samples from n = 10 horses in four preservation treatments at room temperature for up to 150 h and assessed the resulting impact on microbial diversity and the differential abundance of taxa. Treatments included “COLD” (samples packaged with a cool pack), “CLX” (2% chlorhexidine digluconate solution), “NAP” (nucleic acid preservation buffer), and “FTA” (Whatman FTA™ cards). The samples were assessed using 16S rRNA gene sequencing after storage for 0, 24, 72, and 150 h at room temperature under the different treatments. The results showed effective preservation of diversity and community structure with NAP buffer but lower diversity (p = 0.001) and the under-representation of Fibrobacterota in the FTA card samples. The NAP treatment inhibited the overgrowth of bloom taxa that occurred by 72 h at room temperature. The COLD, CLX, and NAP treatments were effective in preserving the faecal microbiota for up to 24 h at room temperature, and the CLX and NAP treatments improved the yield of Patescibacteria and Fibrobacterota in some cases. The cold and CLX treatments were ineffective in preventing community shifts that occurred by 72 h at room temperature. These findings demonstrate the suitability of the COLD, NAP, and CLX treatments for the room temperature storage of equine faeces for up to 24 h and of NAP buffer for up to 150 h prior to processing. Full article
(This article belongs to the Special Issue Update on Role of Equine Microbiota in Health & Disease)
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