Innovative Prevention and Technologies for Zoonotic Diseases and Severe Exotic Diseases

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Veterinary Clinical Studies".

Deadline for manuscript submissions: closed (30 November 2024) | Viewed by 1673

Special Issue Editor


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Guest Editor
State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
Interests: flavivirus; diagnose; vaccine; epidemiology

Special Issue Information

Dear Colleagues,

We are delighted to invite you to submit your next publication to the journal Animals for a Special Issue entitled “Innovative Prevention and Technologies for Zoonotic Diseases and Severe Exotic Diseases”.

With the globalization of trade and the increase in people traveling across regions, as well as global climate change and other factors, many zoonotic diseases have emerged and re-emerged, and some zoonotic diseases have broken through the original epidemic range and become a threat to wider regions. There are urgent needs for innovative diagnostic tests for zoonotic diseases, as well as prophylactic vaccines and therapeutic drugs. More than 60% of human pathogens are zoonotic in origin. This includes a wide variety of bacteria, viruses, fungi, protozoa, parasites, and other pathogens This Special Issue will focus on innovative diagnostic methods, as well as the latest advances in vaccines and therapeutic drugs for viral zoonotic diseases and severe exotic diseases. It also includes the latest epidemiological studies of emerging and re-emerging viral zoonotic diseases.

We look forward to receiving your contributions. Both original research articles and comprehensive reviews are welcome.

Dr. Rong-Hong Hua
Guest Editor

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Keywords

  • zoonotic diseases
  • diagnose
  • vaccine
  • epidemiology

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Published Papers (1 paper)

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Research

14 pages, 3102 KiB  
Article
Identification of Two Linear Epitopes on MGF_110-13L Protein of African Swine Fever Virus with Monoclonal Antibodies
by Shu-Jian Zhang, Bei Niu, Shi-Meng Liu, Yuan-Mao Zhu, Dong-Ming Zhao, Zhi-Gao Bu and Rong-Hong Hua
Animals 2024, 14(13), 1951; https://doi.org/10.3390/ani14131951 - 1 Jul 2024
Cited by 3 | Viewed by 1165
Abstract
African swine fever caused by African swine fever virus (ASFV) is an acute, highly contagious swine disease with high mortality. To facilitate effective vaccine development and find more serodiagnostic targets, fully exploring the ASFV antigenic proteins is urgently needed. In this study, the [...] Read more.
African swine fever caused by African swine fever virus (ASFV) is an acute, highly contagious swine disease with high mortality. To facilitate effective vaccine development and find more serodiagnostic targets, fully exploring the ASFV antigenic proteins is urgently needed. In this study, the MGF_110-13L was identified as an immunodominant antigen among the seven transmembrane proteins. The main outer-membrane domain of MGF_110-13L was expressed and purified. Two monoclonal antibodies (mAbs; 8C3, and 10E4) against MGF_110-13L were generated. The epitopes of two mAbs were preliminary mapped with the peptide fusion proteins after probing with mAbs by enzyme-linked immunosorbent assay (ELISA) and Western blot. And the two target epitopes were fine-mapped using further truncated peptide fusion protein strategy. Finally, the core sequences of mAbs 8C3 and 10E4 were identified as 48WDCQDGICKNKITESRFIDS67, and 122GDHQQLSIKQ131, respectively. The peptides of epitopes were synthesized and probed with ASFV antibody positive pig sera by a dot blot assay, and the results showed that epitope 10E4 was an antigenic epitope. The epitope 10E4 peptide was further evaluated as a potential antigen for detecting ASFV antibodies. To our knowledge, this is the first report of antigenic epitope information on the antigenic MGF_110-13L protein of ASFV. Full article
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