Detection of Pesticide Residues in Agricultural Products

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Agricultural Product Quality and Safety".

Deadline for manuscript submissions: 10 July 2026 | Viewed by 900

Special Issue Editors


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Guest Editor
Department of Applied Biology, Dong-A University, Busan 49315, Republic of Korea
Interests: pesticide multiresidue analytical method development; residue monitoring & dietary/occupational risk assessment; pre-harvest residue limits & safe-spraying SOPs; occupational pesticide exposure studies (agricultural workers); xenobiotic biotransformation by metabolic enzymes & soil microbes; environmental fate & degradation pathways of pesticides/xenobiotics; molecular toxicology—docking, mode-of-action, metabolomics & proteomics; bioactive natural-product isolation & characterization; pesticide chemistry & toxicology
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Guest Editor
Department of Biological Environment, School of Natural Resources and Environmental Science, Kangwon National University, Chuncheon 24341, Gangwon-do, Republic of Korea
Interests: pesticide degradation dynamics & predictive modeling; pesticide metabolic pathways & plant effects; environmental pesticide exposure & toxicological risk assessment; mechanisms of pesticide action (insecticidal/fungicidal/herbicidal); natural-product isolation & bioactivity screening; analytical-method development (LC-MS/MS, GC-MS/MS); omics-based mechanistic studies; pesticide chemistry & toxicology

Special Issue Information

Dear Colleagues,

The widespread use of agrochemicals has safeguarded global food supply, yet trace-level residues in agricultural commodities, soils, and irrigation waters remain a critical concern for regulators, producers, and consumers. This Special Issue of Agriculture on the “Detection of Pesticide Residues in Agricultural Products” seeks to serve as a forum for cutting-edge research that strengthens the analytical, technological, and policy foundations of residue monitoring across the entire farm-to-fork continuum. Building on more than a decade of progress—from early GC/LC-MS workflows to today’s high-resolution MS platforms, refined QuEChERS protocols, and UAV-driven residue mapping—this Special Issue aims to showcase innovations that enhance sensitivity, throughput, and ecological relevance. Suitable contributions will include those focused on (i) the development or validation of rapid multiresidue methods (e.g., LC-MS/MS, LC-QTOF, GC-MS/MS, or portable MS); (ii) the design of targeted high-sensitivity assays for difficult-to-analyze (“challenging”) pesticides, such as highly polar or thermolabile compounds; (iii) the characterization of residue dynamics, drift, and dissipation following conventional or drone spraying; (iv) the establishment/suggestion of the maximum residue level (MRL), pre-harvest interval (PHI), pre-harvest residue limits (PHRLs), or improved dietary risk assessment models; and (v) the integration of non-target screening, metabolomics, or machine learning for holistic exposure assessment. We particularly welcome interdisciplinary studies that link analytical advances with agronomic practices, regulatory frameworks, or toxicological and consumer risk perspectives, spanning crops, agricultural soils, irrigation waters, livestock feed, and edible insects.

Dr. Yongho Shin
Prof. Dr. Jiho Lee
Guest Editors

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Keywords

  • pesticide analysis
  • QuEChERS
  • chromatography
  • mass spectrometry
  • matrix effect
  • UAV spraying
  • pesticide residue
  • agricultural environment
  • food safety monitoring

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Published Papers (1 paper)

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Research

15 pages, 1047 KB  
Article
Desorption-Enhanced QuEChERS Extraction of Tebufenpyrad from Soil and Its Greenhouse Dissipation
by Yoon-Hee Lee, Jae-Woon Baek, Tae-Gyu Min, Da-Geon Lee, Yong-Won Cho, Won-Guen Oh and Yongho Shin
Agriculture 2026, 16(1), 91; https://doi.org/10.3390/agriculture16010091 - 31 Dec 2025
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Abstract
A method for quantifying tebufenpyrad residues in greenhouse sandy loam soils was developed and validated. Given the strong sorption (high Koc) of tebufenpyrad to mineral–organic domains in soils, desorption-limited and partially bound residues may occur, so sample preparation methods should actively [...] Read more.
A method for quantifying tebufenpyrad residues in greenhouse sandy loam soils was developed and validated. Given the strong sorption (high Koc) of tebufenpyrad to mineral–organic domains in soils, desorption-limited and partially bound residues may occur, so sample preparation methods should actively promote desorption to minimize underestimation. The QuEChERS extraction procedure was optimized by adjusting pre-wetting volume and aqueous medium to enhance desorption prior to salt-induced acetonitrile partitioning. Pre-wetting volume markedly affected phase separation and recovery: acceptable ranges were 80.2–82.0% at 5–10 mL, 94.6% at 15 mL, and 99.1% at 20 mL, while a supra-quantitative value of 119.6% was observed at 25 mL, likely due to salt-induced contraction of the acetonitrile layer, which artificially concentrates tebufenpyrad. Among pre-wetting reagents, 15 mL of 0.05% HCl yielded the highest desorption in field soil (0.20 mg/kg), compared with distilled water (0.13 mg/kg), formic acid (0.16 mg/kg), and EDTA (0.14–0.17 mg/kg). The final method employed 15 mL of 0.05% HCl for pre-wetting, followed by acetonitrile extraction and MgSO4/NaCl partitioning. Linearity (r2 = 0.9990) was achieved over 1.25 to 100 ng/mL, with an LOQ of 0.005 mg/kg and average recoveries of 86.7%, 99.8%, and 98.5% at 0.01, 0.1, and 30 mg/kg, respectively (RSD ≤ 6.2%), satisfying SANTE criteria. In greenhouse soil, residues declined from 1.9 to 0.3 mg/kg at the recommended rate (1×) and from 4.8 to 0.7 mg/kg at the doubled rate (2×) within 46 d (DT50 ≈ 20 d). This validated QuEChERS method provides a reliable analytical basis for evaluating tebufenpyrad dissipation in soil. Full article
(This article belongs to the Special Issue Detection of Pesticide Residues in Agricultural Products)
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